Review
Multidisciplinary Sciences
Mohammad Monirul Hasan Tipu, Md. Mostafa Masud, Raunak Jahan, Artho Baroi, A. K. M. A. Hoque
Summary: Citrus greening is a serious disease affecting citrus trees globally, caused by Candidatus Liberibacter bacteria. The disease is spread by the Asian Citrus Psyllid and has no known cure, requiring removal of affected plants to prevent bacterial spread. Detection methods such as the pen test can help farmers identify symptoms and differentiate from nutritional deficiencies.
Article
Horticulture
Ariel Singerman, Stephen H. Futch, Brandon Page
Summary: Citrus greening has significantly reduced sweet orange yield in Florida, impacting the profitability and sustainability of citrus production. Improved rootstocks can increase citrus tolerance to the disease and help growers cope better with its effects. The study's findings can aid growers in making informed decisions on rootstock selection for new groves.
Article
Microbiology
Marcelo L. Merli, Kaylie A. Padgett-Pagliai, Alexandra E. Cuaycal, Lucila Garcia, Maria Rosa Marano, Graciela L. Lorca, Claudio F. Gonzalez
Summary: The study revealed that a potential effector LotP of 'Candidatus Liberibacter asiaticus' may interact with chaperones like GroEL and Hsp40 to enhance Lon protease activity, leading to damage in citrus plant tissues. LotP may also interact with plant proteins, indicating its potential role in protease activity.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Plant Sciences
Alissar Cheaib, Lamiaa M. Mahmoud, Christopher Vincent, Nabil Killiny, Manjul Dutt
Summary: This study investigates the impact of introducing Vitis vinifera mybA1 (VvmybA1) in 'Hamlin' sweet orange trees. Results show that the transgenic plants display intense purple color due to the accumulation of anthocyanins in leaf epidermal cells. Although there is a slight reduction in photosynthetic performance, other parameters remain unaffected.
Article
Plant Sciences
Amit Levy, Taylor Livingston, Chunxia Wang, Diann Achor, Tripti Vashisth
Summary: In Florida, the majority of citrus trees are affected by Huanglongbing (HLB), caused by Candidatus Liberibacter asiaticus (CLas). Our study focused on the parameters of HLB-affected sweet orange trees and found that a nutritional treatment improved yield and maintained canopy density for over three years. We also determined that canopy density was an efficient trait for predicting fruit yield in HLB-affected trees.
Article
Plant Sciences
Rong-Zhou Qiu, Shao-Ping Chen, Mei-Xiang Chi, Rong-Bo Wang, Ting Huang, Guo-Cheng Fan, Jian Zhao, Qi-Yong Weng
Summary: In this study, a deep learning-based automatic identification model for detecting citrus greening disease was developed. The model achieved high accuracy in recognizing the five symptoms of the disease and demonstrated good generalization performance under different imaging conditions. The model also showed better detection performance for experienced users and can be used as a preliminary screening tool. Researchers developed a user-friendly app called "HLBdetector" that allows farmers to quickly detect citrus greening disease using just a mobile phone without the need for expert guidance.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemical Research Methods
Saeed Hosseinzadeh, Steven A. Higgins, John Ramsey, Kevin Howe, Michael Griggs, Louela Castrillo, Michelle Heck
Summary: Diaphorina citri, the vector of Candidatus Liberibacter asiaticus (CLas) associated with citrus greening disease, exhibits two color morphotypes, blue and non-blue, with differing characteristics in transmission efficiency and immunity-associated proteins. The proteomic profiles differ between the color morphs, with non-blue morphs showing higher mortality in response to entomopathogenic fungus but no differences in microbial load. These findings suggest that color polymorphism in D. citri may play a role in disease transmission and resistance under certain conditions.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Plant Sciences
Joon Hyuk Suh, Anirban Guha, Zhixin Wang, Sheng-Yang Li, Nabil Killiny, Christopher Vincent, Yu Wang
Summary: This study demonstrates that shading can alleviate the detrimental effects of Huanglongbing (HLB) on citrus trees. Through metabolomics and physiological approaches, the underlying metabolic mechanisms related to shade-induced morpho-physiological changes in citrus were investigated. Moderate shading prevents excessive starch accumulation, increases foliar sucrose levels, and promotes plant growth and fruit yield.
Article
Plant Sciences
Kim D. Bowman, Greg McCollum, Danelle K. Seymour
Summary: This study aims to identify superior rootstocks for commercial use and map important traits for the next generation of outstanding rootstocks through the performance evaluation of 50 new hybrid rootstocks and commercial standards. The results suggest that there is a significant genetic component underlying rootstock-mediated tolerance to HLB and other critical traits, and marker-based breeding approaches can be used for the rapid selection of next-generation rootstocks with desired traits for commercial success.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Agriculture, Multidisciplinary
Chengcheng He, Xiaobin Li, Yunshi Liu, Biyun Yang, Zhiwei Wu, Shipei Tan, Dapeng Ye, Haiyong Weng
Summary: The study aimed to develop a handheld device that captures multicolor fluorescence and multispectral reflectance images and utilize deep learning and transfer learning technologies for citrus Huanglongbing (HLB) disease detection. The results demonstrated the effectiveness of the developed device in detecting HLB disease in different infected statuses and cultivars with high accuracy.
COMPUTERS AND ELECTRONICS IN AGRICULTURE
(2022)
Article
Horticulture
Nirmal Timilsina, Ozgur Batuman, Fernando Alferez, Davie Kadyampakeni, Ruby Tiwari, Ramdas Kanissery
Summary: The study evaluated the impact of a popular preemergence herbicide, diuron, on young Hamlin and Valencia orange trees. The results showed that diuron generally had no significant impact on citrus root growth and fruit detachment force. However, Valencia trees treated with high doses of diuron showed higher severity of Huanglongbing disease. Inconsistent results were observed for Hamlin trees treated with low and medium doses of diuron. Overall, the study suggests that diuron can be a safe option for preemergence weed suppression in citrus production when used in accordance with recommended dosage and restrictions.
Article
Plant Sciences
Bo Li, Yi Zhang, Dewen Qiu, Frederic Francis, Shuangchao Wang
Summary: The study revealed that HLB infection suppresses photosynthesis and induces starch biosynthesis in citrus plants, with defense-associated proteins being more extensively regulated in symptomatic tissues. Changes in salicylic and jasmonic acid levels are positively correlated with phytohormone biosynthesis-related proteins, and key responsive genes are identified for potential tolerance and resistance breeding against Ca. L. asiaticus.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Horticulture
Eliezer Louzada, Omar Vazquez, Sandy Chavez, Mamoudou Setamou, Madhurababu Kunta
Summary: This research found the most reliable method for detecting only live CLas cells in citrus by testing primers amplifying various amplicon sizes.
Article
Chemistry, Multidisciplinary
Ioannis E. Papadakis, Chrysovalantou Antonopoulou, Thomas Sotiropoulos, Christos Chatzissavvidis, Ioannis Therios
Summary: Magnesium is essential for the growth and development of plants, and its deficiency has become a concern for citrus orchards worldwide. This study investigated the effects of magnesium deficiency on various aspects of orange plants and emphasized the importance of magnesium management in citrus farming. It provides insights into increasing magnesium concentrations, understanding magnesium absorption mechanisms, and optimizing nutrient management in sweet orange trees.
APPLIED SCIENCES-BASEL
(2023)
Article
Agriculture, Multidisciplinary
Xin Liu, Zhixin Wang, Frederick G. Gmitter Jr, Jude W. Grosser, Yu Wang
Summary: In this study, a combined strategy of untargeted metabolomics and machine learning was used to investigate the effects of rootstocks on the metabolites of HLB-affected sweet orange juices. A total of 2531 ion features were detected using high-resolution mass spectrometry, and 54 metabolites were identified as differential markers based on the random forest algorithm. The results revealed that rootstocks might regulate the physiological response against HLB by affecting glutamate, aspartate, proline, and arginine metabolism, as well as phenylalanine and phenylpropanoid metabolism. These findings provide new insights on the effects of rootstocks on the metabolites of HLB-affected sweet orange juices.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Microbiology
Pedro L. Ramos-Gonzalez, Camila Chabi-Jesus, Aline D. Tassi, Renata Faier Calegario, Ricardo Harakava, Claudia F. Nome, Elliot W. Kitajima, Juliana Freitas-Astua
Summary: In this study, molecular and biological features of three new viruses of the family Kitaviridae were described through various analyses. These viruses are transmitted by Brevipalpus mites, which infect plants. The study also identified the Brevipalpus mite species associated with these viruses.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Aline Daniele Tassi, Pedro Luis Ramos-Gonzalez, Thais Elise Sinico, Elliot Watanabe Kitajima, Juliana Freitas-Astua
Summary: This study investigated the transmission mechanism of the plant virus CiLV-C by Brevipalpus mites. Through direct observation using an electron microscope, the researchers proposed the hypothesis of viral movement through intercellular spaces in the mites and described the presence of viral particles in the mite's body. The study also suggested potential genes that may control the circulation of the virus within the mite.
FRONTIERS IN MICROBIOLOGY
(2022)
Editorial Material
Plant Sciences
Pedro Luis Ramos-Gonzalez, Hideki Kondo, Sergey Morozov, Nikolaos Vasilakis, Arvind Varsani, Mengji Cao, Juliana Freitas-Astua
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Peter J. Walker, Juliana Freitas-Astua, Nicolas Bejerman, Kim R. Blasdell, Rachel Breyta, Ralf G. Dietzgen, Anthony R. Fooks, Hideki Kondo, Gael Kurath, Ivan Kuzmin, Pedro Luis Ramos-Gonzalez, Mang Shi, David M. Stone, Robert B. Tesh, Noel Tordo, Nikos Vasilakis, Anna E. Whitfield
Summary: The family Rhabdoviridae consists of viruses with negative-sense RNA genomes and can infect a wide range of hosts including plants, animals, and arthropods. They are important pathogens for humans, livestock, and agricultural crops.
JOURNAL OF GENERAL VIROLOGY
(2022)
Letter
Chemistry, Analytical
Fabiola Manhas Verbi Pereira
BRAZILIAN JOURNAL OF ANALYTICAL CHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Matheus Luis Docema, Tatiana de Souza Moraes, Lisia Borges Attilio, Joao Paulo Rodrigues Marques, Liliane Cristina Liborio Stipp, Ricardo Harakava, Juliana Freitas-Astua, Jose Belasque Junior, Ricardo Antunes Azevedo, Salete Aparecida Gaziola, Francisco de Assis Alves Mourao Filho
Summary: Currently, there are no resistant sweet orange cultivars to citrus canker. Transgenic plants expressing antimicrobial peptides and resistance genes from tolerant cultivars were evaluated for resistance to Xanthomonas citri subsp. citri (Xcc). Transgenic plants showed reduced incidence and severity of citrus canker, as well as lower Xcc titers. Higher superoxide dismutase activity in transgenic plants led to increased hydrogen peroxide production. Callose deposition, an important plant defense response, was observed in transgenic plants with lower Xcc populations.
PLANT CELL TISSUE AND ORGAN CULTURE
(2023)
Review
Plant Sciences
Pedro Luis Ramos-Gonzalez, Gabriella Dias Arena, Aline Daniele Tassi, Camila Chabi-Jesus, Elliot Watanabe Kitajima, Juliana Freitas-Astua
Summary: Kitaviridae is a family of plant-infecting viruses that have unique localized infections and show deficient movement. They are transmitted by mites and infect a wide range of host plants, causing economic damage to crops.
ANNUAL REVIEW OF PHYTOPATHOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Flavio A. Carvalho, Fernando B. Oda, Luis V. S. Sacramento, Fabiola M. V. Pereira, Eduardo J. Crevelin, Antonio E. M. Crotti, Andre G. Santos
Summary: This study evaluated the chemical composition and variability of the essential oil (EO) of Casearia sylvestris leaves. Variations in chemical components were found among different varieties and within the same variety. The main compounds in the EO were beta-elemene, alpha-humulene, germacrene D, bicyclogermacrene, spathulenol, caryophyllene oxide, and humulene epoxide II. Additionally, different varieties of C. sylvestris exhibited variations in trunk fissures and leaf morphology.
Article
Food Science & Technology
Giovanna Nalhiati, Gabriel Goncalves Borges, Marco Aurelio Speranca, Fabiola Manhas Verbi Pereira
Summary: This study presents a feasible analytical method for quality control of red alcohol vinegar without the need for dilutions, filtration, or expensive instrumentation. The method eliminates the requirement for adding chemicals. A total of 30 sample solutions were prepared using a 2(2) central composite design (CCD) to produce vinegar with good quality. Analyzing the data from square images converted into color histograms, multivariate classification models were calculated, and they exhibited a reliability higher than 0.9 for accuracy, sensitivity, and specificity. This low-cost and user-friendly analytical method can be useful in identifying operational errors and out-of-specification end-products.
FOOD ANALYTICAL METHODS
(2023)
Article
Biotechnology & Applied Microbiology
Silvia de Oliveira Dorta, Lisia Borges Attilio, Odimar Zanuzo Zanardi, Joao Roberto Spotti Lopes, Marcos Antonio Machado, Juliana Freitas-Astua
Summary: The Asian citrus psyllid (ACP) is the vector of the devastating disease huanglongbing (HLB) in citrus. Previous research has shown the potential of using Bt strains containing cry/cyt genes to control ACP nymphs. This study attempted to produce transgenic sweet orange plants expressing the cry11A gene from Bt, and confirmed the presence and copy number of the transgene using PCR and Southern blot analyses. Greenhouse mortality assays showed that the tested events had effects ranging from 22% to 43% on D. citri nymphs after a five-day exposure period. This is the first report of citrus plants expressing the Bt cry11A gene for the management of D. citri nymphs.
JOURNAL OF BIOTECHNOLOGY
(2023)
Article
Plant Sciences
Camila Chabi-Jesus, Pedro Luis Ramos-Gonzalez, Aline Daniele Tassi, Laura Rossetto Pereira, Marines Bastianel, Douglas Lau, Maria Cristina Canale, Ricardo Harakava, Valdenice Moreira Novelli, Elliot Watanabe Kitajima, Juliana Freitas-Astua
Summary: Citrus leprosis (CL) is a viral disease that seriously affects Brazilian citriculture. In this study, a new virus called citrus bright spot virus (CiBSV) was discovered in sweet orange trees affected by CL in small orchards in Southern Brazil. Sequence analysis revealed that CiBSV belongs to the genus Dichorhavirus and clusters with citrus leprosis virus N, which is transmitted by Brevipalpus phoenicis. Brevipalpus papayensis and B. azores were found in CiBSV-infected citrus plants, with B. azores being the only vector capable of transmitting the virus to Arabidopsis plants.
Article
Chemistry, Multidisciplinary
Dennis da Silva Ferreira, Leticia da Silva Rodrigues, Fabiola Manhas Verbi Pereira, Edenir Rodrigues Pereira-Filho
Summary: This tutorial provides a step-by-step guide on using principal component analysis (PCA) to handle big datasets. Examples of chemical elements' concentration, emission spectrum, and energy-dispersive X-ray fluorescence (EDXRF) of e-waste were used. Five routines were proposed for data processing and PCA calculation, focusing on laser-induced breakdown spectroscopy (LIBS), EDXRF, and heat maps preparation. The tutorial can be implemented in various softwares like MatLab, Octave, R, and Python. Three examples of applying PCA were given, including concentrations and spectra, as well as an example of creating heat maps for a hyperspectral image. A YouTube playlist was also created for further reference. The authors aim to contribute to research in this field.
Article
Plant Sciences
Gabriella D. Arena, Pedro L. Ramos-Gonzalez, Aline D. Tassi, Marcos A. Machado, Juliana Freitas-Astua
Summary: This study developed two TaqMan RT-qPCR assays for detecting and quantifying Citrus leprosis virus C (CiLV-C) lineage SJP, which is prevalent in the Brazilian citrus belt and the world's main sweet orange production area. The assays targeted two specific genes, p29 and RdRp, and were proven to be efficient and sensitive. By quantifying the target genes in different tissues and organisms, the study revealed the distribution and accumulation of CiLV-C molecules, the higher quantities in sweet orange fruits compared to stems and leaves, as well as the progressive increase during the early stages of infection. These findings contribute to a better understanding of the epidemiology and virus-vector interaction of citrus leprosis.
TROPICAL PLANT PATHOLOGY
(2023)
Article
Plant Sciences
Jessika Angelotti-Mendonca, Perla Novais de Oliveira, Nathalia Felipe Ansante, Liliane Cristina Liborio Stipp, Juliana Freitas-Astua, Fernando Manuel Matias Hurtado, Jose Belasque, Francisco de Assis Alves Mourao Filho
Summary: Researchers investigated the role of the CsEDS5 gene in the resistance of transgenic plants by transforming it into Micro-Tom tomato plants. They found that some transgenic plants exhibited higher resistance to X. vesicatoria, which could be significant in mitigating the impact of citrus canker.
TROPICAL PLANT PATHOLOGY
(2022)