Article
Food Science & Technology
Ummuhan Cakmak, Fulya Oz Tuncay, Yakup Kolcuoglu
Summary: The amylase obtained from Cladophora hutchinsiae was purified and characterized in terms of its properties and applications. The enzyme showed optimal activity at pH 6 and 10°C, and demonstrated remarkable pH stability. It could be used in the presence of certain organic solvents and cations. Additionally, the enzyme was effective in releasing reducing sugar from fruit juices and fish feed, suggesting its potential applications in various industries.
Article
Energy & Fuels
Amira A. Matrawy, Ahmed Khalil, Amira M. Embaby
Summary: This study aims to produce cold-adapted alpha-amylase (CA-AM21) using the zero-cost inducer bread waste and the marine psychrotolerant Glutamicibacter soli strain AM6 EMCCN 3074. By optimizing various factors, such as incubation temperature, incubation time, and medium composition, the production and purification of CA-AM21 were successfully improved. The purified CA-AM21 showed excellent washing efficiency with commercial laundry detergents, indicating its potential application in detergent formulations.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Physiology
Karen R. Kelly, Laura J. Arrington, Jake R. Bernards, Andrew E. Jensen
Summary: Long-duration cold water diving induces a stress response and affects thermoregulation.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Engineering, Chemical
Mauro Marengo, Davide Pezzilli, Eleonora Gianquinto, Alex Fissore, Simonetta Oliaro-Bosso, Barbara Sgorbini, Francesca Spyrakis, Salvatore Adinolfi
Summary: Alpha-amylases are enzymes with a wide distribution and high sequence identity, but their catalytic activities have diverged throughout evolution. By comparing alpha-amylases from human, Aspergillus oryzae, and pancreatic porcine, we found that despite structural homology, these enzymes exhibit strikingly different activities mediated by different ions. This highlights the need to carefully consider these functional differences and establish proper experimental conditions when using alpha-amylases from different organisms as models for human enzymes.
Article
Biotechnology & Applied Microbiology
Junhua Zhang, Xuehua Yu, Bo Guan, Youzhen Hu, Xu Li, Jun Zeng, Yongqing Ni
Summary: In this study, a cold-adapted trehalase enzyme was isolated and characterized from a psychrotolerant bacterium. The enzyme showed high catalytic activity and specificity, suggesting its potential applications in various industries.
FERMENTATION-BASEL
(2022)
Review
Microbiology
Mansi Chauhan, Ayushi Kimothi, Avinash Sharma, Anita Pandey
Summary: The cold adapted microorganisms, psychrophiles/psychrotolerants, undergo cellular and biochemical modifications to cope with low temperature stress. These microorganisms play a crucial role in various ecological processes in cold environments. The functional capabilities of microorganisms in cold environments are determined by their strategies in cell metabolism, physicochemical constraints, and stress factors. Pseudomonas spp., a predominant group of microorganisms in cold environments, have been extensively studied for their plant growth promotion, biocontrol abilities, and biotechnological applications. This review summarizes the functional versatility of Pseudomonas, with a focus on their peculiarities and ecological and biotechnological applications.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Environmental Sciences
Lijun Shen, Sitong Zhang, Guang Chen
Summary: This study summarized a series of cold response strategies for microorganisms, including regulation of cell membrane fluidity, synthesis of cold adaptation proteins, and metabolic changes, as well as the potential of biocatalysts produced by cold-adapted microorganisms. These findings provide guidance for expanding the application values of cold-adapted microorganisms.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Cong Liu, Xia Gong, Xiao Yang, Zipei Yu, Weihao Li, Guangyi Liao, Chuanquan Lin, Lelun Jiang, Changqing Yi
Summary: This study reports a POCT system for quantitation of salivary alpha-amylase (sAA) with clinically acceptable accuracy in any setting. Preliminary clinical studies using this system reveal the great potential of sAA as an early warning indicator of abnormal glucose metabolism.
Article
Chemistry, Applied
C. F. De Schepper, P. Michiels, C. Buve, A. M. Van Loey, C. M. Courtin
Summary: The study investigated the activity and inactivation kinetics of α-amylase and β-amylase during barley malt mashing, finding that temperature had an impact on the activity differences among different varieties, but these differences were not reflected in the resulting wort composition after mashing in the actual process.
CARBOHYDRATE POLYMERS
(2021)
Article
Microbiology
Marcelo Baeza, Sergio Zuniga, Vicente Peragallo, Fernando Gutierrez, Salvador Barahona, Jennifer Alcaino, Victor Cifuentes
Summary: Microorganisms have developed various strategies to adapt to cold environments, including the synthesis of protective molecules, regulation of proteins and membrane fluidity. The response to cold can vary among microorganisms and is influenced by the characteristics of the cold environment and seasonal temperature changes.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Zhengfeng Yang, Zhendi Huang, Qian Wu, Xianghua Tang, Zunxi Huang
Summary: Cold-adapted proteases have advantages such as high catalytic efficiency at low temperature and low energy input, making them economically and ecologically valuable. However, their application potential has not been fully developed, which has hindered their promotion in the industry.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Microbiology
Yehui Liu, Na Zhang, Jie Ma, Yuqi Zhou, Qiang Wei, Chunjie Tian, Yi Fang, Rongzhen Zhong, Guang Chen, Sitong Zhang
Summary: Cold-adapted enzymes are enzymes with catalytic activity at low temperatures, high temperature sensitivity, and the ability to adapt to cold stimulation. They are derived from animals, plants, and microorganisms in polar areas, mountains, and the deep sea. Cold-adapted enzymes have been widely used in areas such as human and other animal food production, environmental protection and restoration, and fundamental biological research. Among them, cold-adapted enzymes derived from microorganisms are particularly attractive due to their short production cycles, high yield, and simple separation and purification.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Pia Staric, Jure Mravlje, Miran Mozetic, Rok Zaplotnik, Barbara Setina Batic, Ita Junkar, Katarina Vogel Mikus
Summary: Cold plasma (CP) technology is a novel technique used to modify the surface properties of various materials. This study explored the effects of CP treatment on wheat seeds and found that it altered the seed morphology, decreased germination speed, and affected seedling root development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Zhou-Hua Cheng, Jie Wu, Jia-Qi Liu, Di Min, Dong-Feng Liu, Wen-Wei Li, Han-Qing Yu
Summary: In this study, we developed an optimized CRISPR-associated transposase system called ShCAST, which enables efficient transposition of ultra-long DNA sequences onto bacterial chromosomes. The engineered strain exhibited improved substrate diversity and extracellular electron transfer ability, expanding the application range and flexibility of genetic engineering techniques.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Multidisciplinary Sciences
Indeewarie H. Dissanayake, Upeka Bandaranayake, Lakshika R. Keerthirathna, Chamalika Manawadu, Rajitha M. Silva, Boudjelal Mohamed, Ali Rizwan, Dinithi C. Peiris
Summary: This study examines the potential antidiabetic and anticancer properties of the polyphenolic extract from Caulerpa racemosa, a green macroalgae. The results suggest that the extract exhibits pharmacological potential and could be used as a lead compound for developing antidiabetic and anticancer drugs.
SCIENTIFIC REPORTS
(2022)
