Article
Cell Biology
Ann De Maziere, Jan van der Beek, Suzanne van Dijk, Cecilia de Heus, Fulvio Reggiori, Masato Koike, Judith Klumperman
Summary: This study presents an optimized procedure for localizing endogenous LC3 at ultrastructural resolution. It provides researchers with a tool to study the canonical and non-canonical roles of LC3 in native conditions.
Review
Biology
Biswajoy Ghosh, Krishna Agarwal
Summary: Optical microscopes have revolutionized our understanding of life by pushing the limits of speed, quality, and observable space in biological specimens. While label-based microscopy has been widely used in life science research, the use of label-free microscopy has been limited. This article discusses the integrative potential and applications of label-free optical microscopes for unperturbed analysis of biological samples.
COMMUNICATIONS BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Mei Wang, Asher C. Leff, Yue Li, Taylor J. Woehl
Summary: This study successfully demonstrated the in situ controlled synthesis of alloyed AuCu nanocrystals under LP-TEM, while maintaining the molecular structure of metal thiolate complexes. The research revealed that polymer capping ligands not only have known functions during LP-TEM synthesis, but also prevent copper oxidation and facilitate the formation of prenucleation cluster intermediates.
Review
Chemistry, Multidisciplinary
Alexander R. R. Hendricks, Bradley F. F. Guilliams, Rachel S. S. Cohen, Tony Tien, Gavin A. A. McEwen, Kanda M. M. Borgognoni, Christopher J. J. Ackerson
Summary: When a protein/peptide controls the synthesis of an inorganic nanoparticle, a cloneable NanoParticle (cNP) is created. The properties of the cNP are determined by the protein sequence encoded in DNA. Modification of the DNA encoding a cNP can alter its properties. The DNA encoding a cNP can be transferred into any organism, enabling recombinant production of cNPs with defined properties.
CHEMICAL COMMUNICATIONS
(2023)
Article
Biology
Shailey Gale Twamley, Anke Stach, Heike Heilmann, Berit Soehl-Kielczynski, Verena Stangl, Antje Ludwig, Agnieszka Munster-Wandowski
Summary: This study evaluated a method to preserve and label sensitive GCX components for high-resolution microscopy analysis. The aim was to better understand the role of the GCX in disease development and progression. High-resolution microscopy proved to be a powerful tool for visualizing the ultra-small components and biological interactions of the GCX.
Article
Multidisciplinary Sciences
Takahito Sanada, Junji Yamaguchi, Yoko Furuta, Soichiro Kakuta, Isei Tanida, Yasuo Uchiyama
Summary: In this study, researchers successfully established a proximity-labeled in-resin correlative light-electron microscopy (CLEM) method for Epon-embedded cells using a biotin ligase called miniTurbo. By labeling intracellular biotinylated proteins with fluorophore-conjugated streptavidin, they were able to preserve the ultrastructures of organelles in 100-nm ultrathin sections of Epon-embedded cells. The fluorescence signals remained stable and bright for up to 14 days, and the technique proved to be superior in performance for in-resin CLEM.
SCIENTIFIC REPORTS
(2022)
Review
Chemistry, Organic
Thorge Reiber, Oleksandr Zavoiura, Christian Dose, Dmytro A. Yushchenko
Summary: Cell analysis techniques like flow cytometry and fluorescence microscopy require bright and specific staining reagents to generate strong fluorescence signal. Significant research has been done in the field of fluorophore development, but modifying biomolecules with multiple emitters remains challenging.
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Philipp M. Pelz, Catherine Groschner, Alexandra Bruefach, Adam Satariano, Colin Ophus, Mary C. Scott
Summary: The shape-controlled synthesis of multiply twinned nanostructures is crucial in nanoscience. This study investigates the prevalence of transient structures during the growth of multiply twinned particles and employs atomic electron tomography to reveal the atomic-scale three-dimensional structure of a Pd nanoparticle undergoing a shape transition.
Article
Biochemistry & Molecular Biology
Ilzira A. Minigalieva, Yuliya V. Ryabova, Ivan G. Shelomencev, Lev A. Amromin, Regina F. Minigalieva, Yuliya M. Sutunkova, Larisa I. Privalova, Marina P. Sutunkova
Summary: We investigated the effects of Fe2O3 nanoparticles on the brain structure and function in rats. Electron microscopy revealed the presence of Fe2O3 nanoparticles in the olfactory bulb tissues but not in the brain's basal ganglia. We observed an increase in axons with damaged myelin sheaths and pathologically altered mitochondria in the exposed animals' brains. Our findings suggest that Fe2O3 nanoparticles can have a toxic effect on the central nervous system even at low doses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Materials Science, Multidisciplinary
M. Sangeetha Vidhya, Fuad Ameen, Turki Dawoud, R. Yuvakkumar, G. Ravi, P. Kumar, Dhayalan Velauthapillai
Summary: Nanostructured zinc oxide prepared by hydrothermal route exhibits morphology and optical properties that are significantly influenced by doping concentration. Oxygen vacancies in zinc oxide play a key role in anticancer mechanism, emitting green light.
Article
Chemistry, Multidisciplinary
Rebecca C. Miller, Roy H. Geiss, Amy L. Prieto
Summary: Understanding the microstructure of complex crystal structures is crucial for controlling material properties in next-generation devices. In this study, a synthetic route to controllably twinned olivine nanoparticles was reported, with microstructural characterization of Fe2GeS4 nanoparticles demonstrating the formation of triplets of twins. The work highlights the potential for future exploration in solution-phase synthetic approaches to control microstructure in compositionally complex, technologically relevant structures.
Article
Chemistry, Multidisciplinary
Teodora Andrian, Pietro Delcanale, Silvia Pujals, Lorenzo Albertazzi
Summary: The functionalization of nanoparticles with functional moieties is important for achieving cell targeting in nanomedicine. The correlation between size and ligand number in nanoparticles is crucial for understanding structure-activity relations. A new method combining super-resolution microscopy and transmission electron microscopy, called super-resCLEM, has been introduced to accurately measure these parameters and address the issue of heterogeneity in nanoparticle populations.
Article
Nanoscience & Nanotechnology
Yuyang Wang, Paul E. D. Soto Rodriguez, Laura Woythe, Samuel Sanchez, Josep Samitier, Peter Zijlstra, Lorenzo Albertazzi
Summary: Nanoparticles have great potential for applications in nanomedicine and molecular biosensing. The protein corona formed due to nonspecific interactions between particles and proteins plays a vital role in the fate of nanoparticles in vivo and affects their performance as biosensors. In this study, a super-resolution microscopic approach called stimulated emission depletion (STED) microscopy was used to investigate the protein corona on single silica nanoparticles and their interactions with cells. The results showed that STED microscopy can reveal the structural features of the protein corona, such as its distribution on the particle surface and the extent of protein internalization in porous particles. Moreover, the composition of the protein corona at the single-particle level was determined using multicolor measurements. It was also found that the composition varies between particles and is influenced by the particle geometry. The study demonstrates the potential of STED microscopy in understanding the mechanistic aspects of protein coronas and in the design of nanoparticles for nanomedicine and biosensing.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Analytical
Alessandra Gianoncelli, Katarina Vogel-Mikus, Murielle Salome, Ernesto Pascotto, Giuseppe Ricci, Lorella Pascolo
Summary: Although cryogenic conditions are ideal for single-cell and plant analyses, it is still challenging to cryo-fix human or animal soft tissue for X-ray fluorescence analysis. In this study, we compare and discuss the analysis of bovine ovarian tissues under cryogenic or room temperature conditions, with the latter being either chemically fixed or freeze-dried.
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
(2023)
Article
Multidisciplinary Sciences
Lunda Shen, Zhaoming Su, Kailu Yang, Cheng Wu, Thomas Becker, Deborah Bell-Pedersen, Junjie Zhang, Matthew S. Sachs
Summary: The study elucidated the mechanism of action of CHX in inhibiting protein synthesis by arresting the ribosome in the classical PRE translocation state, without interfering with A-site reactivity.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cell Biology
Anand N. Rao, Aditi Falnikar, Eileen T. O'Toole, Mary K. Morphew, Andreas Hoenger, Michael W. Davidson, Xiaobing Yuan, Peter W. Baas
JOURNAL OF CELL BIOLOGY
(2016)
Article
Biochemistry & Molecular Biology
Joanna R. Brown, Cindi L. Schwartz, Johil. M. Heumann, Scott C. Dawson, Andreas Hoenger
JOURNAL OF STRUCTURAL BIOLOGY
(2016)
Review
Biochemistry & Molecular Biology
Eva A. Rog-Zielinska, Callum M. Johnston, Eileen T. O'Toole, Mary Morphew, Andreas Hoenger, Peter Kohl
PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY
(2016)
Article
Infectious Diseases
Johanna L. Hoeoeg, Sylvain Lacomble, Cedric Bouchet-Marquis, Laura Briggs, Kristin Park, Andreas Hoenger, Keith Gull
PLOS NEGLECTED TROPICAL DISEASES
(2016)
Article
Cell Biology
Chris N. Goulbourne, Peter Gin, Angelica Tatar, Chika Nobumori, Andreas Hoenger, Haibo Jiang, Chris R. M. Grovenor, Oludotun Adeyo, Jeffrey D. Esko, Ira J. Goldberg, Karen Reue, Peter Tontonoz, Andre Bensadoun, Anne P. Beigneux, Stephen G. Young, Loren G. Fong
Article
Biochemistry & Molecular Biology
Vassiliki A. Iconomidou, Aristeidis Leontis, Andreas Hoenger, Stavros J. Hamodrakas
Article
Cell Biology
Katherine C. Rank, Chun Ju Chen, Julia Cope, Ken Porche, Andreas Hoenger, Susan P. Gilbert, Ivan Rayment
JOURNAL OF CELL BIOLOGY
(2012)
Article
Biochemistry & Molecular Biology
Miguel A. Gonzalez, Julia Cope, Katherine C. Rank, Chun Ju Chen, Peter Tittmann, Ivan Rayment, Susan P. Gilbert, Andreas Hoenger
JOURNAL OF STRUCTURAL BIOLOGY
(2013)
Article
Multidisciplinary Sciences
Julia Cope, Katherine C. Rank, Susan P. Gilbert, Ivan Rayment, Andreas Hoenger
Article
Plant Sciences
Andreas Hoenger
Article
Microbiology
Kimberly D. Erickson, Cedric Bouchet-Marquis, Katie Heiser, Eva Szomolanyi-Tsuda, Rabinarayan Mishra, Benjamin Lamothe, Andreas Hoenger, Robert L. Garcea
Article
Biology
Johanna L. Hoeoeg, Sylvain Lacomble, Eileen T. O'Toole, Andreas Hoenger, J. Richard McIntosh, Keith Gull
Article
Biology
Minghua Liu, Maria B. Heimlicher, Mirjam Bachler, Chieze C. Ibeneche-Nnewihe, Ernst-Ludwig Florin, Damian Brunner, Andreas Hoenger
Article
Cell Biology
Maria B. Heimlicher, Mirjam Bachler, Minghua Liu, Chieze Ibeneche-Nnewihe, Ernst-Ludwig Florin, Andreas Hoenger, Damian Brunner
JOURNAL OF CELL SCIENCE
(2019)