期刊
JOURNAL OF MICROMECHANICS AND MICROENGINEERING
卷 25, 期 5, 页码 -出版社
IOP PUBLISHING LTD
DOI: 10.1088/0960-1317/25/5/055015
关键词
paper; rapid prototyping; PDMS bonding; cell culture; air-liquid interface
类别
资金
- National Science Foundation under the EFRI Program [1240443]
- Directorate For Engineering
- Emerging Frontiers & Multidisciplinary Activities [1240443] Funding Source: National Science Foundation
- Biotechnology and Biological Sciences Research Council [BB/H011293/1, BB/I02643X/1] Funding Source: researchfish
- National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs) [NC/K500318/1] Funding Source: researchfish
- BBSRC [BB/I02643X/1, BB/H011293/1] Funding Source: UKRI
A commercially available Janus paper with one hydrophobic (polyethylene-coated) face and a hygroscopic/hydrophilic one is irreversibly bonded to a polydimethylsiloxane (PDMS) substrate incorporating microfluidic channels via corona discharge surface treatment. The bond strength between the polymer-coated side and PDMS is characterized as a function of corona treatment time and annealing temperature/time. A maximum strength of 392 kPa is obtained with a 2 min corona treatment followed by 60 min of annealing at 120 degrees C. The water contact angle of the corona-treated polymer side decreases with increased discharge duration from 98 degrees to 22 degrees. The hygroscopic/hydrophilic side is seeded with human lung fibroblast cells encapsulated in a methacrylated gelatin (GelMA) hydrogel to show the potential of this technology for nutrient and chemical delivery in an air-liquid interface cell culture.
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