Article
Physiology
Young-Yeon Choo, Tsuyoshi Sakai, Satoshi Komatsu, Reiko Ikebe, Ann Jeffers, Karan P. Singh, Steven Idell, Torry A. Tucker, Mitsuo Ikebe
Summary: By investigating the mechanism of contractile myofibroblast differentiation of PMCs, this study found that calponin 1 plays a central role in the formation of stress fibers and focal adhesions, which contribute to the stiffness of scar tissue in the pleura and promote pleural fibrosis (PF).
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Tsuyoshi Sakai, Young-yeon Choo, Osamu Sato, Reiko Ikebe, Ann Jeffers, Steven Idell, Torry Tucker, Mitsuo Ikebe
Summary: Pleural mesothelial cells (PMCs) play a central role in pleural fibrosis, transitioning into myofibroblasts and producing extracellular matrix proteins such as Fibronectin (FN1). This study reveals that Myosin 5b (Myo5b) is critical in the transportation and secretion of FN1 from human pleural mesothelial cells (HPMCs), with TGF-beta regulating the association between Myo5b and FN1. This finding highlights the importance of Myo5b and Rab11b in FN1 transportation and secretion, potentially contributing to the development of pleural fibrosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Shouichi Okamoto, Hiroki Ebana, Masatoshi Kurihara, Keiko Mitani, Etsuko Kobayashi, Takuo Hayashi, Yasuhito Sekimoto, Koichi Nishino, Mizuto Otsuji, Toshio Kumasaka, Kazuhisa Takahashi, Kuniaki Seyama
Summary: Birt-Hogg-Dube syndrome (BHDS) is an autosomal dominant inheritance disease caused by folliculin (FLCN) mutations, associated with lung cysts and spontaneous pneumothorax. BHDS patients' pleural mesothelial cells (PMCs) exhibit impaired cellular function, including decreased attachment, proliferation, and migration, as well as increased apoptosis compared to PMCs from primary spontaneous pneumothorax (PSP) patients. Additionally, gene expression and AMPK/LKB1 activation are significantly altered in BHDS-PMCs, suggesting the involvement of FLCN haploinsufficiency in affecting cellular function.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Li-Ling Zhou, Pei-Pei Cheng, Xin-Liang He, Li-Mei Liang, Meng Wang, Yu-Zhi Lu, Lin-Jie Song, Liang Xiong, Fei Xiang, Fan Yu, Xiaorong Wang, Jian-Bao Xin, Peter A. Greer, Yunchao Su, Wan-Li Ma, Hong Ye
Summary: Idiopathic pulmonary fibrosis (IPF) is characterized by fibrosis in the peripheral or sub-pleural area, with migration of pleural mesothelial cells (PMC) contributing to the fibrotic process. Calpain plays a key role in PMC migration and activation of calpain leads to cell migration, proliferation, and collagen synthesis. Inhibition of calpain attenuates pulmonary fibrosis and prevents PMC migration, indicating its potential as a therapeutic target for IPF.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Zihan Yang, Zhihang Zhou, Tongxu Si, Zhengdong Zhou, Li Zhou, Y. Rebecca Chin, Liang Zhang, Xinyuan Guan, Mengsu Yang
Summary: Cancer metastasis is a major cause of cancer-related death, and it is characterized by excessive extracellular matrix deposition and increased stiffness in solid tumors. The confined migration of tumor cells in these conditions plays a crucial role in metastasis, but inhibitors specifically targeting this type of migration are rare. In this study, a microfluidic chip was designed to screen for drugs that can effectively inhibit confined migration of cancer cells. By applying this chip, three novel inhibitors of confined migration, MA-5, SB-705498, and diphenyleneiodonium chloride, were discovered in multiple cancer types. The mechanisms of action of these inhibitors were found to be targeting mitochondria, actin polymerization, and cell viability, respectively. Overall, a high-throughput microfluidic platform for screening drugs targeting confined migration was established, and three effective inhibitors were identified.
Article
Chemistry, Medicinal
Wei-Lin Chen, Mei-Chuan Chen, Shang-Fu Hsu, Shih-Hsin Hsiao, Chi-Li Chung
Summary: The study found that HDACi can reduce the overproduction of PAI-1 and collagen induced by LTA, thereby alleviating pleural fibrosis by inhibiting JNK. This treatment may offer potential therapy for pleural fibrosis caused by GPB PPE.
Article
Chemistry, Analytical
Franciele Flores Vit, Rony Nunes, Yu Tzu Wu, Marco Cesar Prado Soares, Naiara Godoi, Eric Fujiwara, Hernandes F. Carvalho, Lucimara Gaziola de la Torre
Summary: This study introduces a microfluidic technology for drug screening that enables the assessment of cellular behavior in dynamic conditions and the recovery of cells for subsequent processing and imaging.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biochemistry & Molecular Biology
Giulia Matusali, Flavia Trionfetti, Veronica Bordoni, Roberta Nardacci, Laura Falasca, Daniele Colombo, Michela Terri, Claudia Montaldo, Concetta Castilletti, Davide Mariotti, Franca Del Nonno, Maria Rosaria Capobianchi, Chiara Agrati, Marco Tripodi, Raffaele Strippoli
Summary: This study reveals that pleural mesothelial cells (MCs) in COVID-19 patients express ACE2 and may play a potential role in promoting fibrotic reactions and regulating immune responses. The findings provide insight into the pathogenesis of lung fibrosis in COVID-19 and suggest MCs as important players in the disease process.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Engineering, Electrical & Electronic
Yuheng Huang, Meng Nie, Bozhi Wu, Binghui Li, Kuibo Yin, Litao Sun
Summary: A novel dual-drive MEMS device integrated with both a thermal actuator and an electrostatic actuator is proposed for convenient in-situ electro-mechanical test of nanomaterials. The experiment results indicate that this device allows for agile selection of drive modes to analyze different mechanical properties and verify test accuracy through self-contrast.
SENSORS AND ACTUATORS A-PHYSICAL
(2022)
Article
Anatomy & Morphology
Francesca Bodega, Chiara Sironi, Luciano Zocchi, Cristina Porta
Summary: The study investigated the role of sialomucin in friction reduction by studying the sliding friction of pleural mesothelial cells under different experimental conditions. Results showed that sialomucin may play a role in reducing the friction of these cells.
Article
Chemistry, Analytical
Amirreza Malekanfard, Shayesteh Beladi-Behbahani, Tzuen-Rong Tzeng, Hui Zhao, Xiangchun Xuan
Summary: This study introduces a technique for particle/cell focusing using low-frequency AC electric fields, demonstrating its effectiveness compared to DC iDEP focusing with a second-order power relationship for improved efficiency. The AC iDEP focusing method eliminates the need for large electric fields and small constrictions, offering better performance benefits.
ANALYTICAL CHEMISTRY
(2021)
Article
Cell Biology
Ilenia Masi, Valentina Caprara, Francesca Spadaro, Lidia Chellini, Rosanna Sestito, Andrea Zancla, Alberto Rainer, Anna Bagnato, Laura Rosano
Summary: The study reveals that the endothelin A receptor (ETAR) drives invadopodia through beta-arr1, and high EDNRNILK expression is associated with poor clinical outcomes in serous ovarian cancer (SOC).
Article
Materials Science, Multidisciplinary
Michaela Welch, Deborah A. Flusberg, Peter Hsi, Nerses J. Haroutunian, Jose A. Santos, Ernest S. Kim, Stacey Markovic, Jonathan R. Coppeta, Charles A. Lissandrello, Jenna L. Balestrini, Vishal Tandon
Summary: Autologous cellular therapies have shown great success in treating hematological cancers and have potential for various indications. However, manufacturing these therapies at a rapid pace and low cost remains challenging, especially due to the genetic modification of target cells using expensive viral vectors. This study presents a microfluidic continuous-flow electroporation device that offers high throughput processing, high performance, and potential for automation, making it suitable for large-scale clinical manufacturing.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Infectious Diseases
Shuanglinzi Deng, Xinyue Hu, Lisha Luo, Wei Tang, Yuanyuan Jiang, Feifei Yin, Chengping Hu, Juntao Feng, Xiaozhao Li
Summary: The study demonstrated the crucial role of anaphylatoxins in orchestrating Th17 responses through interactions between CD16(+) monocytes and pleural mesothelial cells in tuberculous pleural effusion. Anaphylatoxins induced the secretion of cytokines promoting Th17 expansion, along with enhancing costimulatory molecule expression in CD16(+) monocytes, ultimately highlighting the potential of targeting CD16(+) monocytes to control inflammation in TPE.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Article
Cell Biology
M. Chauvin, M. -C. Meinsohn, S. Dasari, P. May, S. Iyer, N. M. P. Nguyen, E. Oliva, Z. Lucchini, N. Nagykery, A. Kashiwagi, R. Mishra, R. Maser, J. Wells, C. J. Bult, A. K. Mitra, Patricia K. Donahoe, D. Pepin
Summary: Cancer-associated mesothelial cells (CAMCs) in the tumor microenvironment promote tumor growth and immune evasion. The paracrine axis between cancer cells expressing anti-Mueurollerian hormone (AMH) and CAMCs expressing its receptor AMHR2 plays a critical role. AMHR2 expression is induced by factors secreted by cancer cells during their reprogramming into CAMCs. Overexpression of AMHR2 induces the expression of immunosuppressive cytokines and growth factors that stimulate ovarian cancer cell growth in an AMH-dependent way. The AMH/AMHR2 axis regulates the pro-tumoral function of CAMCs in ovarian cancer.
Article
Chemistry, Analytical
Peng Zheng, Sujan Kasani, Weirui Tan, Jennifer Boryczka, Xuefei Gao, Feng Yang, Nianqiang Wu
Summary: An ultrasensitive plasmonic near-infrared fluorescent biosensor substrate has been developed for the detection of the important protein biomarker GFAP in traumatic brain injury. By using a near-infrared fluorophore and a plasmonic gold nanopyramid array, the biosensor achieves enhanced fluorescence signals and overcomes interference from the blood plasma matrix.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Jianbo Sun, Changjiang Li, Yuqiang Hu, Yawen Ding, Tongbo Wu
Summary: A rapid, sensitive, and selective biosensor for UDG activity detection has been developed in this study based on the substrate preference of Lambda exonuclease. The proposed strategy exhibited high selectivity and sensitivity, and has been successfully applied to detect UDG in real biological samples and screen UDG inhibitors.
Article
Engineering, Chemical
Zhengchao Xi, Jianbo Sun, Lei Chen, Haixia Cui, Yanjun Ma, Huidi Zhou, Jianmin Chen
Summary: Natural serpentine powders were incorporated into phosphate-bonded solid coatings to enhance anti-wear performance. Through experiments, it was found that the optimal incorporation of natural serpentine in phosphate coatings was 10 wt.%, resulting in significantly elevated anti-wear performance. Furthermore, under harsher friction conditions, the strengthening effect of natural serpentine on the tribological performance of phosphate composite coatings was further stimulated.
Review
Energy & Fuels
Hui Yang, Nianqiang Wu
Summary: This review article discusses the ionic conductivity of solid-state electrolytes for lithium batteries, including the mechanisms of ion conduction and various electrolyte materials. It also highlights strategies to improve the ionic conductivity.
ENERGY SCIENCE & ENGINEERING
(2022)
Correction
Soil Science
Chimin Lai, Fei Peng, Jianbo Sun, Jun Zhou, Chengyang Li, Xingliang Xu, Xiaojie Chen, Quangang You, Hongyang Sun, Jian Sun, Xian Xue, Hans Lambers
BIOLOGY AND FERTILITY OF SOILS
(2023)
Article
Soil Science
Chimin Lai, Fei Peng, Jianbo Sun, Jun Zhou, Chengyang Li, Xingliang Xu, Xiaojie Chen, Quangang You, Hongyang Sun, Jian Sun, Xian Xue, Hans Lambers
Summary: The study found that there is niche differentiation and niche overlap between plants and microorganisms in the uptake of soil nitrogen during the degradation process of alpine meadows on the Qinghai-Tibetan Plateau. Plants and microorganisms showed different preferences for available soil nitrogen in different stages of degradation, which plays a role in maintaining the stability of community net primary productivity.
BIOLOGY AND FERTILITY OF SOILS
(2023)
Article
Environmental Sciences
Fei Peng, Chimin Lai, Chengyang Li, Chen Ji, Pengfei Zhang, Jianbo Sun, Xiaojie Chen, Quangang You, Xian Xue
Summary: This study conducted a clipping experiment on the Qinghai-Tibetan Plateau to investigate the compensatory growth and conditions for the occurrence of over-compensatory at alpine meadows with different degradation status. The results showed that competition for light and reduction of soil nitrogen are the limiting factors for plant growth after clipping at non-degraded, slightly degraded, and heavily degraded alpine meadows. Additionally, it was found that over-compensatory growth occurred mainly due to the performance of forbs at moderately degraded alpine meadow.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2023)
Article
Geochemistry & Geophysics
Haiyan Wu, Ting Liang, Chao Gao, Yanjun Wu, Hongxia Cao, Jun Yu, Fenfei Bai, Jianbo Sun, Keyong Li, Yanqin Guo
Summary: A study was conducted on the Yan'an gas field to examine the natural gas reserves, diagenetic evolution, and different diagenesis environments. The results showed the lithology of the reservoir and the filling materials, as well as the carbon and oxygen isotope values and Sr-87/Sr-86 values. The stratigraphic development indicated a cyclical trend, and the fluid inclusions revealed the sources of fluids in the sedimentation and diagenesis process.
Article
Automation & Control Systems
An Li, Dong Jiang, Jianbo Sun, Zicheng Liu, Christopher H. T. Lee
Summary: This article unifies the vector torque control of reluctance motor with different coil pitch in the classic vector model frameworks. The torque of the reluctance motor is decoupled into synchronous-speed reluctance force (SSRF) and double-speed reluctance force (DSRF) components. The proposed unified vector torque control method is verified by simulations and experiments.
IEEE TRANSACTIONS ON INDUSTRIAL ELECTRONICS
(2023)
Article
Immunology
Xiao Cheng, Li Chi, Tianqiong Lin, Fengyin Liang, Zhong Pei, Jianbo Sun, Wei Teng
Summary: This study found that periodontitis is closely related to the development of Alzheimer's disease (AD). Porphyromonas gingivalis (Pg), a key pathogen in periodontal disease, was found to cause immune overreaction and induce cognitive impairment. The study also showed that monocytic myeloid-derived suppressor cells (mMDSCs) may play a role in immune balance in AD patients with periodontitis, and that exogenous mMDSCs can ameliorate immune overreaction and cognitive impairment caused by Pg infection.
JOURNAL OF NEUROINFLAMMATION
(2023)
Article
Dentistry, Oral Surgery & Medicine
Hang Zou, Niu Zhou, Xiao Cheng, Yi Qiu, Wenhong Hou, Jianbo Sun
Summary: This study revealed that gingipains of Porphyromonas gingivalis are important virulence factors that downregulate B10 cells and alter immune responses.
MOLECULAR ORAL MICROBIOLOGY
(2023)
Article
Nanoscience & Nanotechnology
Zhengchao Xi, Jianbo Sun, Lei Chen, Haixia Cui, Yanjun Ma, Huidi Zhou, Jianmin Chen
Summary: Natural serpentine and nanosized magnesium silicate hydroxide (MSH) were added to phosphate-bonded solid coatings to improve their tribological properties. The study revealed that MSH can significantly enhance the antiwear performance of phosphate coatings compared to natural serpentine due to its larger specific surface area and weaker thermal stability. The structural water in MSH can easily dehydrate and generate dangling bonds on the surface under the combined effect of frictional force and friction temperature. Additionally, MSH has a strong cation replacement ability and can undergo tribochemical reactions with the metal-based worn surface, leading to the formation of a composite protective tribo-film that alleviates direct abrasion.
ACS APPLIED NANO MATERIALS
(2023)
Article
Materials Science, Biomaterials
Sizhe Huang, Sabrina Urbina Villafranca, Iyanah Mehta, Omri Yosfan, Eunji Hong, Anyang Wang, Nianqiang Wu, Qianbin Wang, Siyuan Rao
Summary: This study aims to improve the stability of chemically cross-linked poly(vinyl alcohol) (PVA) hydrogel fibers by using atomic layer deposition (ALD) to coat them with an inorganic silicon dioxide (SiO2) layer. SiO2-coated PVA hydrogel fibers showed superior stability and preserved their mechanical and optical properties compared to uncoated fibers. In vivo experiments demonstrated that these SiO2-coated PVA hydrogel fibers could be used to optically activate the motor cortex and modulate locomotor behaviors in transgenic mice.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Infectious Diseases
Dongchun Dian, Weilong Zhang, Minjun Lu, Yong Zhong, Yurong Huang, Guiling Chen, Zhangquan Chen, Luxin Yu, Jianbo Sun
Summary: This retrospective cohort study found that combining ulinastatin and azithromycin can significantly improve clinical symptoms, reduce inflammatory cytokines, and oxidative stress levels in children with severe pneumonia.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Biochemistry & Molecular Biology
Yongjian Su, Xiaoqing Zhang, Youcheng Liang, Jianbo Sun, Chengyu Lu, Zunnan Huang
Summary: This study explored the changes in immune cell proportions and cell-to-cell interactions in different stages of intestinal-type gastric cancer (IGC) using single-cell RNA-seq data and bulk transcriptome data. The results revealed that in precancerous lesions, immune cell interactions were mainly stimulated by chemokines, while in tumors, intercellular movement of upregulated molecules and amplified signals were associated with tumor necrosis factor family and immunosuppression. Additionally, novel prognostic biomarkers for IGC were identified. The study provides insights into TME heterogeneity and potential targets for precise therapy in IGC.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
