Article
Infectious Diseases
Jin Liang, Yuanfang Hu, Mingxia Fu, Na Li, Fengxia Wang, Xiaojun Yu, Bing Ji
Summary: The study investigated the molecular epidemiology of Staphylococcus aureus (S. aureus) in patients from the Yellow River Delta region and examined antibiotic resistance profiles in different genetic backgrounds. Antibiotic susceptibility testing, spa typing, and agr typing were conducted on all 204 strains. The results identified 39 MRSA strains and 22 hVISA isolates. The majority of MRSA isolates belonged to agrI and were associated with type IV SCCmec. The most common genotypes among MRSA population were ST59-t437-agrI-IVa and ST72-t2431-agrI-IVF. The study highlights the genetic diversity and drug resistance patterns among MSSA strains exhibiting phenotypic hVISA.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Biochemical Research Methods
Yu-Ching Liu, Jang-Jih Lu, Lee-Chung Lin, Hsiao-Chuan Lin, Chao-Jung Chen
Summary: The rapid identification of methicillin-sensitive Staphylococcus aureus (MSSA), heterogeneous vancomycin-intermediate S. aureus (hVISA), and vancomycin-intermediate S. aureus (VISA) is crucial for accurate treatment, timely intervention, and outbreak prevention. Proteomics analysis of 90 S. aureus isolates led to the discovery of protein biomarkers that can discriminate among MSSA, hVISA, and VISA strains, with two VISA, two hVISA, and one MSSA protein biomarkers identified and verified for high sensitivities and specificities. Data is available via MassIVE with identifier MSV000085776.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Cell Biology
Zhimin Bai, Min Chen, Qiaofa Lin, Ying Ye, Hongmei Fan, Kaizhen Wen, Jianxing Zeng, Donghong Huang, Wenfei Mo, Ying Lei, Zhijun Liao
Summary: By extracting feature vectors from protein sequences and using classification tools, MRSA and MSSA were successfully distinguished. MRSA showed high resistance to penicillin and high pathogenic risk. Cross-infection between different types of MRSA was found in Quanzhou, and MRSA in traditional hospitals exhibited increasingly blurred molecular characteristics.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Mengli Jin, Shuyue Zhu, Yating Tang, Xiangri Kong, Xingye Wang, Yufen Li, Shuang Jiang, Lin Wei, Chunjie Hu, Bingmei Wang, Wu Song
Summary: Antimicrobial resistance (AMR) is a global health threat, and there is a need for new anti-infective agents. Ayanin, a flavonoid extracted from Callicarpa nudiflora, was found to be an inhibitor of MRSA ClpP, suggesting its potential therapeutic effect against MRSA infections.
BIOCHEMICAL PHARMACOLOGY
(2023)
Article
Multidisciplinary Sciences
Pooi Yin Chung, Pey Lin Narissa Loh, Hui-min Neoh, Ramliza Ramli
Summary: Staphylococcus aureus is a significant threat to human health and has developed resistance to conventional antimicrobials. The presence of biofilms complicates treatment for infections caused by multi-drug resistant S. aureus. Alpha-amyrin has been shown to reduce biomass and inhibit metabolic activity of S. aureus. This study demonstrates that alpha-amyrin can reduce biofilm biomass of MSSA, MRSA, and VISA isolates, suggesting its potential as a therapeutic agent for chronic S. aureus infections.
Article
Microbiology
Melissa J. Karau, Suzannah M. Schmidt-Malan, Scott A. Cunningham, Jayawant N. Mandrekar, Bobbi S. Pritt, Tiffany R. Keepers, Alisa W. Serio, Surya Chitra, Robin Patel
Summary: Omadacycline, vancomycin, and rifampin, as well as rifampin combination therapies, were evaluated in an experimental rat model of MRSA osteomyelitis. The results showed that rifampin combination therapy was effective in reducing MRSA recovery without developing resistance. The combination of omadacycline and rifampin yielded negative MRSA detection, suggesting its potential as a treatment for osteomyelitis.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Genetics & Heredity
Hongbin Chen, Yuyao Yin, Lucy van Dorp, Liam P. Shaw, Hua Gao, Mislav Acman, Jizhen Yuan, Fengning Chen, Shijun Sun, Xiaojuan Wang, Shuguang Li, Yawei Zhang, Rhys A. Farrer, Hui Wang, Francois Balloux
Summary: The study reveals that in China, the community-associated MRSA strain ST59 is gradually replacing the healthcare-associated strain ST239, mainly due to its higher virulence potential rather than antibiotic resistance or biofilm production. The chemotaxis inhibitory protein (chp) carried by ST59 may be a significant factor contributing to its increased virulence.
Review
Microbiology
Jhih-Hang Jiang, David R. Cameron, Cara Nethercott, Marta Aires-de-Sousa, Anton Y. Peleg
Summary: This review focuses on the mechanisms that trigger the establishment and maintenance of current, dominant MRSA lineages across the globe. Factors that influence MRSA's ability to colonize and cause disease within a host are discussed, with an emphasis on colonization and niche adaptation. Understanding these mechanisms may lead to the development of targeted therapies.
CLINICAL MICROBIOLOGY REVIEWS
(2023)
Article
Microbiology
Alina Dietrich, Ursula Steffens, Mike Gajdiss, Anna-Lena Boschert, Jana Katharina Droege, Christiane Szekat, Peter Sass, Imran T. Malik, Jan Bornikoel, Laura Reinke, Boris Macek, Mirita Franz-Wachtel, Kay Nieselt, Theresa Harbig, Kirstin Scherlach, Heike Broetz-Oesterhelt, Christian Hertweck, Hans-Georg Sahl, Gabriele Bierbaum
Summary: Resistance to antibiotics is a growing problem and alternative antimicrobial therapies are needed. Cervimycin, a polyketide antibiotic, shows promising activity against multidrug-resistant staphylococci and vancomycin-resistant enterococci. This study identified the Clp system and the essential kinase WalK as mutational hotspots for cervimycin resistance in Staphylococcus aureus. Furthermore, cervimycin-resistant strains exhibited vancomycin-intermediate resistance phenotype, suggesting a role of cervimycin in perturbing the bacterial cell envelope.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Monika Kumaraswamy, Kamilla Wiull, Bishnu Joshi, George Sakoulas, Armin Kousha, Gustav Vaaje-Kolstad, Mona Johannessen, Kristin Hegstad, Victor Nizet, Fatemeh Askarian
Summary: This study revealed that MRSA-derived MVs are capable of reducing the pathogen's susceptibility to VAN, thereby increasing its resistance to treatment, particularly in interactions with human neutrophils. Additionally, the elevated expression of proteins associated with antibiotic resistance in MVs may further impact MRSA's resistance.
Article
Food Science & Technology
Martina Florianova, Kristyna Korena, Helena Juricova
Summary: This study evaluated the prevalence of Staphylococcus aureus in dry-fermented salami and found multidrug-resistant and virulent strains. The source of contamination was identified as raw pork and human carriers. Therefore, maintaining high standards of hygiene and manufacturing practices in food processing is crucial.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Veterinary Sciences
Hester Rynhoud, Brian M. Forde, Scott A. Beatson, Sam Abraham, Erika Meler, Ricardo J. Soares Magalhaes, Justine S. Gibson
Summary: This study investigated the molecular epidemiology of MRSA and MRSP isolates in dogs and cats, revealing significant differences in resistance and virulence factors between different sequence types. However, there were no differences in individual and clinical factors between predominant sequence types. The majority of isolates showed high levels of antimicrobial resistance, posing a potential threat to human and veterinary health. The phylogenetic clustering of isolates suggests potential importation of clones from countries with high volume of pet movements, warranting further investigation.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Infectious Diseases
Cheng-En Tsai, Chia-Jui Yang, Yu-Chung Chuang, Jann-Tay Wang, Wang-Huei Sheng, Yee-Chun Chen, Shan-Chwen Chang
Summary: This study aimed to determine the synergistic effects of combining CPT with DAP, VAN, or LNZ against different MRSA strains. The results showed that the CPT-DAP combination exhibited significant synergistic bactericidal effects, while the CPT-VAN and CPT-LNZ combinations did not show synergistic activity. Therefore, DAP may be a better choice for combination therapy with CPT in the treatment of MRSA infections.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Multidisciplinary Sciences
Yifan Rao, Huagang Peng, Weilong Shang, Zhen Hu, Yi Yang, Li Tan, Ming Li, Renjie Zhou, Xiancai Rao
Summary: The WalK(S221P) mutation enhances vancomycin resistance and decreases virulence in Staphylococcus aureus.
JOURNAL OF ADVANCED RESEARCH
(2022)
Article
Immunology
Philip Nikolic, Poonam Mudgil, David G. Harman, John Whitehall
Summary: This study aimed to identify and compare the general differences in protein profiles among clinical strains of S. aureus sensitive and resistant to methicillin. The proteomic analysis revealed differences in protein abundances related to virulence, metabolism, and protein synthesis between MSSA and MRSA strains.
MICROBIAL PATHOGENESIS
(2023)