Article
Evolutionary Biology
Alexandra A. Raspopova, Vladimir S. Lebedev, Jeremy B. Searle, Anna A. Bannikova
Summary: In this study, the phylogenetic relationships within the chromosomally variable Sorex araneus species group were reconstructed using nuclear and mitochondrial genes. The results showed a complex evolutionary history with high incidence of reticulation and possible ancient hybridizations. The authentic mitochondrial lineage of the Iberian shrew S. granarius was found to be closely related to S. coronatus, indicating introgression from S. araneus. This study also revealed deeper reticulations for the first time, suggesting hybrid origins for the S. arcticus and S. coronatus lineages.
Article
Zoology
Eva Ladurner, Paolo Colangelo, Gianni Amori, Federica Lazzeri, Elisa Colonna, Petra Kranebitter
Summary: The study used molecular markers to revise the specimens of the Sorex araneus group in South Tyrol, Italy, detecting the presence of both S. antinorii and S. araneus in the region. This finding underscores the importance of revising museum collections using modern technologies to accurately assess the diversity of small mammal fauna at regional and national levels.
HYSTRIX-ITALIAN JOURNAL OF MAMMALOGY
(2021)
Article
Chemistry, Analytical
Jyothi Basapathi Raghavendra, Thasshwin Mathanlal, Maria-Paz Zorzano, Javier Martin-Torres
Summary: The Earth's atmosphere is important for the transport and dispersal of biological aerosols, but monitoring changes in these communities over time is challenging due to the low microbial biomass in the air. Real-time genomic studies provide a sensitive method for monitoring bioaerosol composition changes, but the low abundance of DNA and proteins in the atmosphere poses a sampling and analyte extraction challenge. In this study, an optimized portable bioaerosol sampler using membrane filters was designed, which can operate autonomously outdoors and capture ambient bioaerosols without user contamination. The sampler demonstrated the ability to recover sufficient DNA for genomic applications, suggesting its potential for continuous environmental monitoring of microbial communities within the air.
Article
Multidisciplinary Sciences
Arielli Fabricio Machado, Camila Duarte Ritter, Cleuton Lima Miranda, Yennie Katarina Bredin, Maria Joao Ramos Pereira, Leandro Duarte
Summary: The study suggests that Amazonia and the Atlantic Forest were connected through multiple dispersion routes in the past, and assesses the potential of mammal species for investigating these connections. The present-day distribution of mammal species in both regions indicates potential for studying past connections between the two forests.
Article
Forestry
Patrick S. Fahey, Rachael M. Fowler, Frank Udovicic, David J. Cantrill, Michael J. Bayly
Summary: This study aimed to investigate the incongruent phylogeographic patterns between plastid DNA and nuclear DNA in Eucalyptus behriana, as well as the influence of chloroplast capture on these patterns. Through genome skimming, chloroplast genomes were sequenced from E. behriana populations and co-occurring species, revealing strong geographic structuring and polyphyly in plastid DNA relationships across different species. This research highlights the widespread phenomenon of chloroplast capture and its impact on phylogeographic patterns in Eucalyptus species.
TREE GENETICS & GENOMES
(2021)
Article
Genetics & Heredity
Jianhui Chen, Tiran Huang, Haili Fan, Fan Lin, Hongping Ma, Jie Cao, Tuanyao Chai, Lanqing Ma, Hong Wang
Summary: In this study, the complete chloroplast genomes of three R. japonica were sequenced and analyzed. The results showed a highly conserved structure of the chloroplast genomes, with detailed annotations of the genes. Phylogenetic and genetic structure analyses revealed the evolutionary characteristics and genetic diversity of R. japonica, and potential molecular markers were identified. This study provides important genetic resources for further development and utilization of R. japonica germplasm, and offers new insights into the evolutionary characteristics of this medicinal plant.
Article
Cell Biology
Siwen Liu, Bobo Wing-Yee Mok, Shaofeng Deng, Honglian Liu, Pui Wang, Wenjun Song, Pin Chen, Xiaofeng Huang, Min Zheng, Siu-Ying Lau, Conor J. Cremin, Chun-Yee Tam, Baiying Li, Liwen Jiang, Yixin Chen, Kwok-Yung Yuen, Honglin Chen
Summary: Host adaptive mutations in the influenza A virus PB2 protein are critical for human infection, with avian PB2 virus inducing higher autophagic flux in infected cells compared to mammalian PB2 virus. The formation of vRNP aggregates is essential for host restriction of avian PB2 IAV, with p62 and its interaction with vRNPs playing a key role in this process. This study reveals a novel strategy for host restriction of avian PB2 IAV through selective autophagic sequestration during late-stage virus replication.
Article
Parasitology
Gabriel Jimenez-Avalos, Alina Soto-Obando, Maria Solis, Robert H. Gilman, Vitaliano Cama, Armando E. Gonzalez, Hector H. Garcia, Patricia Sheen, David Requena, Mirko Zimic, Cysticercosis Working Grp Peru
Summary: This study identified a novel set of 31 non-synonymous mutations that differentiate the main T. solium genotypes, distributed across the genomes of the African-American genotype. Furthermore, the analysis suggested a stratification of the African-American genotypes into an East African and a West African sublineage.
PARASITES & VECTORS
(2023)
Article
Immunology
Martin Kulke, Felix Nagel, Lukas Schulig, Norman Geist, Marcel Gabor, Julia Mayerle, Markus M. Lerch, Andreas Link, Mihaela Delcea
Summary: The study identified two main binding states of the inhibitor to the complex, explicitly relating the influence of the mutation site to conformational changes in the active site of trypsin. Based on the results, a hypothesis was formulated that explains the development of chronic pancreatitis through accelerated digestion of the mutant by trypsin.
JOURNAL OF INFLAMMATION RESEARCH
(2021)
Review
Genetics & Heredity
Rodrigo P. Baptista, Garrett W. Cooper, Jessica C. Kissinger
Summary: Cryptosporidiosis ranks sixth among the most important food-borne parasites globally, contributing significantly to mortality in infants and immunosuppressed individuals. The lack of an optimal in vitro culture system for replicating the parasite's lifecycle makes it challenging to work with, hindering the cloning of isolates. The current sequencing method using oocysts as the lifecycle stage presents difficulties in accurately representing the population due to each oocyst containing multiple progeny.
Article
Parasitology
Svetlana Kornienko, Gergana P. Vasileva, Boyko B. Georgiev
Summary: Neoskrjabinolepis yanchevin is a newly described species of parasite found in common shrews from Bulgaria and Russia, characterized by unique rostellar hooks and claw-like blades, as well as a whip-shaped cirrus with needle-shaped spines. It is differentiated from the other species in the subgenus by its distinctive features.
SYSTEMATIC PARASITOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Christopher Faulkner, Nora H. de Leeuw
Summary: Fentanyl, a potent opioid analgesic, is used in combination with propofol in general anesthesia. Molecular dynamics simulations revealed that fentanyl acts as a stabilizer, helping propofol to remain in binding sites. This study provides insights into the interactions between different drugs in the anesthesia process.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Medicine, Research & Experimental
Moira A. McMahon, Meghdad Rahdar, Swagatam Mukhopadhyay, Huynh-Hoa Bui, Christopher Hart, Sagar Damle, Margo Courtney, Michael W. Baughn, Don W. Cleveland, Frank Bennett
Summary: Antisense oligonucleotides (ASOs) are synthetic nucleic acids that bind to RNA to alter gene expression. A functional genomic screen identified GOLGA8 as a novel regulator that enhances ASO activity, leading to increased uptake and improved splice modulation. GOLGA8 is localized to the trans-Golgi and plasma membrane, and overexpression enhances RNase H1-dependent ASOs as well.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2023)
Article
Biochemistry & Molecular Biology
Zabin K. Bagewadi, U. K. Aakanksha, Deepak A. Yaraguppi, T. M. Yunus Khan, Sanjay H. Deshpande, Manjunath Dammalli, Archana G. Revankar, Anudeep J. Savalagi, Shivaprakash V. Hiremath
Summary: In this study, a novel drug target against Pseudomonas aeruginosa was identified through subtractive genomics and molecular docking studies. Three potential drugs with high binding affinity were screened and their stable and compact interactions with the target protein were confirmed through molecular dynamics simulation.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Biotechnology & Applied Microbiology
Gurusamy Raman, Eun Mi Lee, SeonJoo Park
Summary: Intracellular gene transfer is common among plant genomes, but chloroplast genomes rarely incorporate foreign genetic material due to their high conservation. Convallaria, a genus of monocotyledonous plants, was found to have mitochondrial DNA sequences in the plastome. New plastid molecular genetic markers were developed to differentiate Convallaria species, revealing a separation into Eurasian and North American lineages during the middle Pliocene. Further studies are needed to understand the integration of the MCP region and gene flow in Convallaria.
Article
Zoology
Nikolay A. Shchipanov, Svetlana V. Pavlova
Article
Biochemistry & Molecular Biology
Soraia Barbosa, Joana Pauperio, Svetlana V. Pavlova, Paulo C. Alves, Jeremy B. Searle
MOLECULAR PHYLOGENETICS AND EVOLUTION
(2018)
Article
Plant Sciences
Svetlana Pavlova, Larisa S. Biltueva, Svetlana A. Romanenko, Natalya A. Lemskaya, Anton Shchinov, Alexei Abramov, Viatcheslav V. Rozhnov
COMPARATIVE CYTOGENETICS
(2018)
Article
Zoology
Svetlana V. Pavlova, Nikolay A. Shchipanov
Article
Ecology
N. A. Shchipanov, S. V. Pavlova
RUSSIAN JOURNAL OF ECOLOGY
(2019)
Article
Genetics & Heredity
N. A. Shchipanov, A. A. Kalinin, A. V. Bobretsov, S. V. Pavlova
RUSSIAN JOURNAL OF GENETICS
(2019)
Article
Zoology
Anna A. Bannikova, Paulina D. Jenkins, Evgeniya N. Solovyeva, Svetlana Pavlova, Tatiana B. Demidova, Sergey A. Simanovsky, Boris Sheftel, Vladimir S. Lebedev, Yun Fang, Love Dalen, Alexei Abramov
Article
Zoology
Alexandra A. Raspopova, Anna A. Bannikova, Boris Sheftel, Boris Krystufek, Alexander Kouptsov, Natalia A. Illarionova, Svetlana Pavlova, Vladimir S. Lebedev
Article
Genetics & Heredity
N. A. Shchipanov, A. V. Artamonov, S. V. Titov, S. V. Pavlova
RUSSIAN JOURNAL OF GENETICS
(2020)
Article
Multidisciplinary Sciences
Svetlana A. Romanenko, Vladimir S. Lebedev, Anna A. Bannikova, Svetlana Pavlova, Natalia A. Serdyukova, Natalia Yu Feoktistova, Qu Jiapeng, Sun Yuehua, Alexey Surov, Alexander S. Graphodatsky
Summary: The genus status and phylogenetic relationship of Urocricetus are still debated, but morphological and molecular data show its distinctiveness from other hamsters. Analysis of its karyotype and molecular markers suggest that Urocricetus is a distant relative to Phodopus. The significant reorganization of Urocricetus' karyotype compared to other hamsters indicates that it belongs to a separate divergent lineage within Cricetinae.
SCIENTIFIC REPORTS
(2021)
Article
Zoology
Nikolay A. Shchipanov, Tatiana B. Demidova, Artem Artamonov, Svetlana Pavlova
Summary: A 7-year study in Central Russia found that population density and weather conditions affected the mortality rate of shrews in different habitats. Male shrews outnumber females after overwintering, but decline faster, leading to a female-biased sex ratio in late summer. Shrews that overwinter locally make a significant contribution to genetic patterns and occasional high mortality provides immigrants with opportunities to enhance genetic diversity.
Article
Multidisciplinary Sciences
Svetlana Pavlova, Vladimir S. Lebedev, Vasily D. Yakushov, Yongke Zhu, Yun Fang, Yue-Hua Sun, Boris Sheftel
Summary: Among seven species of the order Eulipotyphla studied cytogenetically in central China, four endemic species from the Qinghai-Tibet Plateau and adjacent mountain ranges were described for the first time, showing unique karyotypic characteristics. In contrast, other species showed no karyotypic polymorphism. The results are consistent with molecular genetic data and suggest differing colonization histories among the species studied.
SCIENTIFIC REPORTS
(2021)
Article
Evolutionary Biology
Sergey N. Matveevsky, Oxana L. Kolomiets, Nikolay A. Shchipanov, Svetlana V. Pavlova
Summary: This study investigated the meiotic process in male hybrid shrews with complex chromosomal configurations using immunocytochemical and electron microscopy methods. Despite abnormalities in chromosome synapsis, morphologically normal spermatozoa were observed, suggesting that the impact of long meiotic configurations on fertility may be minimal.
JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION
(2023)
Article
Plant Sciences
Svetlana V. Pavlova, Sergei A. Borisov, Alexander F. Timoshenko, Boris I. Sheftel
COMPARATIVE CYTOGENETICS
(2017)
Article
Plant Sciences
Sergey N. Matveevsky, Svetlana V. Pavlova, Maret M. Atsaeva, Jeremy B. Searle, Oxana L. Kolomiets
COMPARATIVE CYTOGENETICS
(2017)