期刊
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
卷 167, 期 -, 页码 51-58出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cbpb.2013.10.001
关键词
Diet energy source; Essential fatty acids; Fatty acyl desaturase; PUFA biosynthesis; Solea senegalensis
资金
- FCT (Portugal) [PTDC/MAR/67017/2006]
- European Commission Marie Curie Actions [274184]
- FCT [SFRH/BPD/49051/2008]
- INIA
- LARVANET COST Action [FA0801]
- Fundação para a Ciência e a Tecnologia [PTDC/MAR/67017/2006] Funding Source: FCT
Lipid nutrition of marine fish larvae has focused on supplying essential fatty acids (EFA) at high levels to meet requirements for survival, growth and development. However, some deleterious effects have been reported suggesting that excessive supply of EFA might result in insufficient supply of energy substrates, particularly in species with lower EFA requirements such as Senegalese sole (Solea senegalensis). This study addressed how the balance between EFA and non-EFA (better energy sources) affects larval performance, body composition and metabolism and retention of DHA, by formulating enrichment emulsions containing two different vegetable oil sources (olive oil or soybean oil) and three DHA levels. DHA positively affected growth and survival, independent of oil source, confirming that for sole post-larvae it is advantageous to base enrichments on vegetable oils supplying higher levels of energy, and supplement these with a DHA-rich oil. In addition, body DHA levels were generally comparable considering the large differences in their dietary supply, suggesting that the previously reported Delta 4 fatty acyl desaturase (fad) operates in vivo and that DHA was synthesized at physiologically significant rates through a mechanism involving transcriptional up-regulation of Delta 4fad, which was significantly up-regulated in the low DHA treatments. Furthermore, data suggested that DHA biosynthesis may be regulated by an interaction between dietary n - 3 and n - 6 PUFA, as well as by levels of LC-PUFA, and this may, under certain nutritional conditions, lead to DHA production from C18 precursors. The molecular basis of putative fatty acyl Delta 5 and Delta 6 desaturation activities remains to be fully determined as thorough searches have found only a single (Delta 4) Fads2-type transcript. Therefore, further studies are required but this might represent a unique activity described within vertebrate fads. (C) 2013 Elsevier Inc. All rights reserved.
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