Article
Chemistry, Medicinal
Max Meyrath, Christie B. Palmer, Nathan Reynders, Alain Vanderplasschen, Markus Ollert, Michel Bouvier, Martyna Szpakowska, Andy Chevigne
Summary: The study compared the activation of ACKR3 by ADM and PAMP, finding that PAMP had a stronger potency towards ACKR3 than ADM. Importantly, PAMP-12 was efficiently internalized by ACKR3 without inducing G protein or ERK signaling in vitro.
ACS PHARMACOLOGY & TRANSLATIONAL SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Jordan P. Hempfling, Emily R. Sekera, Amar Sarkar, Amanda B. Hummon, Dehua Pei
Summary: Efficient and convenient method for producing recombinant proteins with free N-terminal cysteines has been reported. Site-specific labeling of the resulting proteins at the N-terminus was achieved through a novel genetic fusion and sequential enzymatic processing.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Review
Endocrinology & Metabolism
J. Michael Conlon
Summary: Enteroglucagon, the predominant peptide released by the intestine in response to nutrients, has been found to have glucagon-like immunoreactivity (GLI) but its physiological function remains unclear.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Health Care Sciences & Services
Silvia Spoto, Josepmaria Argemi, Roberta Di Costanzo, Juan Jose Gavira Gomez, Nahikari Salterain Gonzales, Stefania Basili, Roberto Cangemi, Antonio Abbate, Luciana Locorriere, Francesco Masini, Giulia Testorio, Rodolfo Calarco, Giulia Battifoglia, Fabio Mangiacapra, Marta Fogolari, Sebastiano Costantino, Silvia Angeletti
Summary: Acute heart failure (AHF) is a major cause of hospitalization and mortality worldwide. This study compared the levels of NT-proBNP, MR-proADM, and CRP between patients with AHF and those without AHF and sepsis, and assessed the diagnostic and prognostic value of a multimarker approach in AHF patients. The results showed that MR-proADM and NT-proBNP levels were significantly higher in AHF patients compared to controls, and the combined use of these biomarkers provided predictive value for mortality and re-admission for AHF.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Article
Biochemical Research Methods
Ying Cong, Paul D. Scesa, Eric W. Schmidt
Summary: A key goal of synthetic biology is to modify peptides and proteins through designed modifications, both inside and outside the cell. This study investigates a specific enzyme that can protect the ends of peptides, and shows that mutating the enzyme's active site can expand its substrate range, including reverse geranylation of peptide N-termini.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Biochemical Research Methods
Ying Cong, Paul D. Scesa, Eric W. Schmidt
Summary: A key goal of synthetic biology is to enable the designed and modification of C-Terminal enzymes. AgeMTPT is a selective enzyme that protects the N-terminus of peptides and modifies the C-terminus through enzymatic reactions. The substrate selectivity of this enzyme requires N-terminal aromatic amino acids and tolerates diverse uncharged amino acids in other positions. Mutagenesis of the active site of AgeMTPT expands its substrate scope, including the reverse geranylation of peptide N-termini, revealing potential applications in synthetic biology.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Uttpal Anand, Anna Shteinfer-Kuzmine, Gal Sela, Manikandan Santhanam, Benjamin Gottschalk, Rajaa Boujemaa-Paterski, Ohad Medalia, Wolfgang F. Graier, Varda Shoshan-Barmatz
Summary: The mitochondrial protein VDAC1 plays a crucial role in regulating various cellular processes, including metabolism, apoptosis, and cell signaling. In this study, a short peptide derived from the N-terminal region of VDAC1 was designed to improve cellular stability and activity. The peptide exhibited multiple effects on cancer cells, such as apoptosis induction, autophagy, senescence, and cell adhesion. Furthermore, the peptide altered the expression of proteins associated with cell metabolism and signaling. These findings highlight the importance of VDAC1 in controlling diverse cellular functions through its interaction with other proteins.
Article
Chemistry, Multidisciplinary
Sanhu Gou, Beibei Li, Xu Ouyang, Zufang Ba, Chao Zhong, Jingman Ni
Summary: The research involved modifying anoplin by dendrimerization on the C-terminal and lipidation on the N-terminal to improve antibacterial activity, but with an increase in hemolytic activity. Ultimately, a simple lipopeptide A-C8 with a high therapeutic index was obtained, showing potential as an antibiotic.
Article
Biochemistry & Molecular Biology
Chien- Yang, Jiwoo Kim, Shu-Ou Shan
Summary: The ribosome actively participates in diverse protein biogenesis pathways, including folding, localization, and modifications. It imposes an additional layer of substrate selection during N-terminal methionine excision (NME) in bacteria.
JOURNAL OF MOLECULAR BIOLOGY
(2022)
Article
Chemistry, Applied
Kenya Tamaribuchi, Jiaqi Tian, Kengo Akagawa, Kazuaki Kudo
Summary: Enantioselective Michael addition of beta-dicarbonyl compounds towards nitroalkenes was achieved using an immobilized, N-terminal-guanidinylated peptide catalyst. Various nucleophiles and electrophiles were employed, with the resin-supported catalyst being recyclable for five cycles. This method shows promise for industrial applications.
ADVANCED SYNTHESIS & CATALYSIS
(2022)
Article
Chemistry, Organic
Asif Fazal, Jake Wheeler, Michael E. Webb, Ryan F. Seipke
Summary: SurE is a peptide cyclase essential for the production of surugamide antibiotics. However, the substrate specificity of this enzyme is not well understood. In order to address this issue, researchers developed a new assay to investigate the chemical space of the N-terminal substrate residue.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2022)
Article
Chemistry, Physical
Lili Zhang, Siyu Jin, Mengxue Ren, Cheng Lu, Feng Peng, Gennady L. Gutsev
Summary: The magnesium-lanthanum-hydrogen systems show good stability and high hydrogen storage capacity, providing new insights for the design and synthesis of novel hydrogen storage materials.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2022)
Article
Chemistry, Organic
Xiao-Lei Xie, Jing-Ze Qi, Xiao-Cui Wan, Suo-De Zhang, Yan-Ni Zhang, Ge-Min Fang
Summary: We present a robust and practical method for chemical protein synthesis using an o-nitrobenzyl group as a temporary protective group. Through further investigation, we establish a reliable strategy for the quantitative photodeprotection of the o-nitrobenzyl group. This stable protective group is successfully applied in the chemical synthesis of a programmed death ligand 1 fragment, providing a practical approach for hydrazide-based native chemical ligation.
Article
Chemistry, Multidisciplinary
Tularam Sahu, Mohan Kumar, T. K. Sajeev, Manas Joshi, Ram Kumar Mishra, Vishal Rai
Summary: The study demonstrates the specific generation of N-Gly in native proteins for protein tagging without perturbing their structure and function.
CHEMICAL COMMUNICATIONS
(2022)
Review
Plant Sciences
Carmela Giglione, Thierry Meinnel
Summary: N-terminal protein alpha-acetylation (NTA) is a widespread protein modification recently attracting attention, with new findings in photosynthetic organisms revealing unique features and important biological roles.
TRENDS IN PLANT SCIENCE
(2021)
Article
Fisheries
Makoto Kusakabe, Shigenori Nobata, Kaede Saito, Kiriko Ikeba, Sanae Ogasawara, Kiyoshi Tanaka, Yoshio Takei, Susumu Hyodo
Article
Multidisciplinary Sciences
Yuki Minegishi, Marty Kwok-Shing Wong, Takashi Kanbe, Hitoshi Araki, Tomomi Kashiwabara, Minoru Ijichi, Kazuhiro Kogure, Susumu Hyodo
Article
Endocrinology & Metabolism
Emily A. Wolverton, Marty Kwok-Shing Wong, Perry E. Davis, Brianne Hoglin, Ingo Braasch, Robert M. Dores
GENERAL AND COMPARATIVE ENDOCRINOLOGY
(2019)
Article
Multidisciplinary Sciences
Yukitoshi Katayama, Yoshio Takei, Makoto Kusakabe, Tatsuya Sakamoto
SCIENTIFIC REPORTS
(2019)
Article
Cell Biology
Marty Kwok-Shing Wong, Mizuki Uchida, Takehiro Tsukada
CELL AND TISSUE RESEARCH
(2020)
Article
Cell Biology
Yukitoshi Katayama, Marty Kwok-Shing Wong, Makoto Kusakabe, Megumi Fujio, Natsuki Takahashi, Miku Yaguchi, Takehiro Tsukada
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2020)
Article
Microbiology
Keng Po Lai, Xiao Lin, Nathan Tam, Jeff Cheuk Hin Ho, Marty Kwok-Shing Wong, Jie Gu, Ting Fung Chan, William Ka Fai Tse
ENVIRONMENTAL MICROBIOLOGY
(2020)
Article
Biology
Yuki Honda, Wataru Takagi, Marty K. S. Wong, Nobuhiro Ogawa, Kotaro Tokunaga, Kazuya Kofuji, Susumu Hyodo
JOURNAL OF EXPERIMENTAL BIOLOGY
(2020)
Article
Zoology
Naotaka Aburatani, Wataru Takagi, Marty Kwok-Sing Wong, Mitsutaka Kadota, Shigehiro Kuraku, Kotaro Tokunaga, Kazuya Kofuji, Kazuhiro Saito, Waichiro Godo, Tatsuya Sakamoto, Susumu Hyodo
ZOOLOGICAL SCIENCE
(2020)
Article
Multidisciplinary Sciences
Marty Kwok-Shing Wong, Mako Nakao, Susumu Hyodo
SCIENTIFIC REPORTS
(2020)
Review
Endocrinology & Metabolism
Yoshio Takei
Summary: Guanylyl cyclase (GC) is an enzyme that produces cGMP, a second messenger for intracellular signal transduction. pGCs perceive environmental factors and transmit information into cells through cGMP, while sGC is a receptor for nitric oxide and carbon monoxide.
GENERAL AND COMPARATIVE ENDOCRINOLOGY
(2022)
Review
Zoology
Yoshio Takei
Summary: Adaptation to a hypertonic marine environment is crucial for animal survival, with marine teleosts being able to compensate for water loss by absorbing water from the surrounding seawater. Eels, known for their euryhalinity, are commonly used in laboratories for osmoregulation research, providing insights into molecular mechanisms of ion and water transport in the digestive tract. Recent advancements in genome databases have enabled a better understanding of these mechanisms at the molecular level.
ZOOLOGICAL LETTERS
(2021)
Article
Cell Biology
Yukitoshi Katayama, Ami Saito, Maho Ogoshi, Yousuke Tsuneoka, Takao Mukuda, Morio Azuma, Makoto Kusakabe, Yoshio Takei, Takehiro Tsukada
Summary: The cloning of cnp genes from the brain of eel and zebrafish reveals different expressions of cnp4b and cnp4a in different brain regions. This suggests a subfunctionalization of duplicated cnp4s in ancestral teleosts, with cnp4b potentially involved in swallowing and respiration functions.
CELL AND TISSUE RESEARCH
(2022)
Article
Endocrinology & Metabolism
Yoshio Takei, Masaaki Ando, Marty K. S. Wong, Takehiro Tsukada
Summary: This study investigates the molecular mechanisms of transepithelial Cl- transport in the intestine of seawater-acclimated eels, and reveals that GN stimulates Cl- secretion. The DNDS-sensitive anion exchanger (AE) and Na+-HCO3- cotransporter (NBC) play a crucial role in Cl- uptake at the basolateral side, while an unknown DPC-sensitive Cl- channel (ClC) is responsible for Cl- secretion at the mucosal side.
GENERAL AND COMPARATIVE ENDOCRINOLOGY
(2022)
Article
Biology
Shigenori Nobata, Yoshio Takei
Summary: In this study, it was found that isotocin is the potent dipsogenic hormone in eels, while vasopressin and oxytocin are not dipsogenic in mammals. Isotocin induces copious drinking through its action on the AP, while AngII may induce drinking through its action on a different circumventricular site.
JOURNAL OF EXPERIMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Fuqiang Ge, Qiaoling Yu, Jun Zhang, Yaoyao Han, Dongfa Zhu, Xi Xie
Summary: The E93 gene was identified and found to be widely distributed in adult tissues of the swimming crab. The expression levels of PtE93 mRNA in Y-organ and epidermis fluctuated during the molt cycle, suggesting its involvement in juvenile molting. Treatment with 20E induced PtE93 expression, while treatment with MF showed the opposite effect. The study also observed a negative correlation between PtE93 and ecdysteroid biosynthesis genes.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Manuel Marrero, Oscar Monroig, Jose A. Perez, Monica B. Betancor, Ana Galindo, Ana Bolanos, N. Guadalupe Acosta, Covadonga Rodriguez
Summary: This study investigated the combined effects of partial dietary replacement of fish oil (FO) by vegetable oil (VO) and reduced ambient salinity on the fatty acid composition of C. labrosus juveniles. The results showed that C. labrosus is able to biosynthesise EPA and DHA from ALA, and low salinity conditions can upregulate the gene expression related to fatty acid metabolism. Fish reared on FO20 diet showed the highest amount of n-3 LC-PUFA in muscle.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Davide Degli Esposti, Aureline Lalouette, Karen Gaget, Louveline Lepeule, Zineb Chaabi, Maxime Lepretre, Anabelle Espeyte, Nicolas Delorme, Herve Queau, Laura Garnero, Federica Calevro, Arnaud Chaumot, Olivier Geffard
Summary: Metal pollution has significant effects on aquatic environments, and metallothioneins play important roles in maintaining metal homeostasis and detoxification. This study identified two metallothionein-coding transcripts in Gammarus fossarum and investigated their organ-specific expression patterns. The results showed that mt1 was more strongly induced after cadmium exposure, and mt1 was more highly induced in the caeca compared to the gills for any metal exposure. Additionally, mt2 was more inducible in the gills than in the caeca for cadmium and zinc exposure at the individual level.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Cuihong You, Shuangshuang Miao, Zhiyong Xie, Siyuan Lin, Shuqi Wang, Cuiying Chen, Li Lin, Yanhua Huang, Meng Zhou, Yewei Dong, Yuanyou Li, Ping Zhuang
Summary: The rabbitfish S. guttatus was found to have the capability for LC-PUFA biosynthesis, with the expression of related genes varying temporally and spatially during embryogenesis and being influenced by ambient salinity.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Saydur Rahman, Mohammad Maruf Billah, Victor Rangel, Esmirna Cantu
Summary: Global warming has severe impacts on the reproductive fitness of marine invertebrates. In this study, the researchers used various techniques to investigate the effects of high temperatures on global DNA methylation, cellular apoptosis, and nitrative stress in the gonads of Atlantic sea urchins.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
E. S. Kladchenko, A. A. Tkachuk, M. S. Podolskaya, A. Yu. Andreyeva
Summary: This study investigated the osmotic stability and cellular responses of hemocytes in Mediterranean mussel and Pacific oyster. The results revealed that both species showed cellular volume changes and alterations in mitochondrial membrane potential upon hypoosmotic swelling. These findings provide insights into the cellular responses of bivalve mollusks to osmotic stress.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Yuya Ohhara, Chiemi Sagisaka, Kimiko Yamakawa-Kobayashi
Summary: This study investigated the biosynthetic capacity of n-3 PUFAs in the terrestrial invertebrate Sinella dubiosa, and found that it can metabolize oleic, linolenic, and arachidonic acids to produce EPA, suggesting that it may serve as an EPA source in soil ecosystems.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Correction
Biochemistry & Molecular Biology
N. Perales-Garcia, D. Tovar-Ramirez, M. G. Martinez-Morales, B. P. Ceballos-Vazquez, D. A. Corona-Rojas, M. A. Salcedo-Meza, A. Garrido-Mora, F. Vega-Villasante, H. Nolasco-Soria
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
N. Perales-Garcia, D. Tovar-Ramirez, M. G. Martinez-Morales, B. P. Ceballos-Vazquez, D. A. Corona-Rojas, M. A. Salcedo-Meza, A. Garrido-Mora, F. Vega-Villasante, H. Nolasco-Soria
Summary: This study provides the first report on the digestive tract pH of the pygmy octopus. The pH of the digestive tract regions was found to be lower than 7.0 and did not differ significantly between wild and captive octopuses. The digestive gland had the lowest pH values, but they were still above 5.0.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Carlos Espirito-Santo, Carmen Alburquerque, Francisco A. Guardiola, Rodrigo O. A. Ozorio, Leonardo J. Magnoni
Summary: Swimming has physiological effects on farmed fish, but the potential link between swimming and oxidative stress is still poorly understood. This study investigated the impact of different moderate swimming conditions on the antioxidant status of gilthead seabream, revealing that swimming modulated the activity of antioxidant enzymes in the liver and skeletal muscle. Adequate swimming conditions were found to minimize oxidative stress in gilthead seabream.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Yingxin Zhang, Chaobin Qin, Junli Wang, Liping Yang, Xiao Yan, Shaoyang Zhi, Guoxing Nie
Summary: The study focuses on the expression patterns of PFK genes in response to glucose, insulin, and glucagon stimuli in grass carp. The findings suggest that PFK family genes have different or even opposite expression patterns in various tissues, which may contribute to glucose intolerance in fish.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Jianshuang Li, Likang Lyu, Haishen Wen, Yun Li, Xiaojie Wang, Yijia Yao, Xin Qi
Summary: This study investigated the transcriptional regulation of estrogen receptors (ESRs) on isotocin receptors (itrs) in guppies, and found that estrogen can regulate oxytocin receptor expression in ovoviviparous teleosts. The study also demonstrated that ESR1 represses itr2 promoter activity in an estrogen-dependent manner, while overexpression of ESR2a or ESR2b enhances itr2 promoter activity.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Wan-Xin Li, Li-Ting Cai, Yan-Ping Huang, Yuan-Qing Huang, Shu-Hui Pan, Zhi-Long Liu, Charles Brighton Ndandala, Gang Shi, Si-Ping Deng, Hong-Juan Shi, Guang-Li Li, Dong-Neng Jiang
Summary: In this study, it was found that S. argus has two lep genes, with lepa highly expressed in the liver and lepb not detected in any tissue. lepa was found to play a role in regulating female reproduction in S. argus.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Li-Xiang Wu, Xiao-Ying Tan, Yi-Chuang Xu, Hua Zheng, Xiao-Lei Wei, Wu-Hong Lv, Zhi Luo
Summary: This study investigates the mechanisms underlying the activation of lipophagy in yellow catfish under high glucose-induced lipid deposition. The SIRT1-NRF2-TFEB pathway is proposed to mediate lipophagy activation, and lipophagy helps alleviate high glucose-induced lipid deposition. This study provides insights into the control of lipophagy in response to metabolic stress.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)