Article
Multidisciplinary Sciences
Ambreen Tauseef, Asima Karim, Gulfam Ahmad, Qurratulann Afza Gardner, Muhammad Waheed Akhtar
Summary: This study characterized differentially expressed proteins in malignant ovarian tissue, identifying 17 upregulated and 27 downregulated proteins, including six novel proteins not previously reported in ovarian cancer. These proteins may serve as potential novel biomarkers for early diagnosis and prognosis of ovarian cancer.
Article
Multidisciplinary Sciences
Sharifah Zakiah Syed Sulaiman, Wei Miao Tan, Rozanaliza Radzi, Intan Nur Fatiha Shafie, Mokrish Ajat, Rozaihan Mansor, Suhaila Mohamed, Norasfaliza Rahmad, Angela Min Hwei Ng, Seng Fong Lau
Summary: This study compared the proteomic changes in the synovial fluid of surgically and chemically induced osteoarthritis (OA) models. The surgical model showed a wider range of protein changes, while the chemical model had slower OA progression.
Article
Biochemical Research Methods
Masataka Nakagawa, Yui Tomioka, Chiaki Sakuma, Yasunori Kurosawa, Takashi Shibata, Tsutomu Arakawa, Teruo Akuta
Summary: A new protocol for conducting two-dimensional (2D) electrophoresis was developed by combining agarose native gel electrophoresis with sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) or flat SDS agarose gel electrophoresis. The technique utilizes His/MES buffer during the first-dimensional agarose native gel electrophoresis to visualize basic and acidic proteins in their native states or complex structures. The gel strip from the first-dimensional agarose gel electrophoresis is soaked in SDS and placed on top of vertical SDS-PAGE gels or flat SDS-MetaPhor high-resolution agarose gels for the second-dimensional separation.
Article
Biochemistry & Molecular Biology
Zeyad H. Nafaee, Balint Hajdu, Eva Hunyadi-Gulyas, Bela Gyurcsik
Summary: The nuclease domain of colicin E7 can cleave DNA non-specifically. In this study, it was found that Zn2+ ion is the native metal ion of the enzyme and cannot be replaced by other metal ions such as Ni2+, Cu2+ and Cd2+. The binding of Ni2+ ions significantly increases the nuclease activity of the enzyme, while the presence of equivalent Zn2+ ions decreases the activity.
Article
Polymer Science
Fei Ding, Caili Dai, Yongpeng Sun, Guang Zhao, Qing You, Yifei Liu
Summary: Gel conformance control technology is widely used in moderate and high temperature reservoirs, but there are few studies on shallow low-temperature and high-salinity reservoirs. The PHRO gel, composed of gelatinizing agent (polyacrylamide), crosslinking agents (hexamethylenetetramine and resorcinol), and crosslinking promoting agent (oxalic acid), was developed to overcome the difficulties of low-temperature crosslinking and poor stability at high salt concentrations. The PHRO gel showed good salt resistance properties and is suitable for conformance control of low-temperature and high-salinity reservoirs.
Article
Chemistry, Physical
Stefania Bergamini, Elisa Bellei, Luigi Generali, Aldo Tomasi, Carlo Bertoldi
Summary: This study evaluated proteomic changes induced by CHX and CHX+ADS in supragingival dental sites susceptible to discoloration effects. The results showed that long-term use of CHX led to changes in the TSCM proteome, but these changes were reduced when using CHX+ADS. The ADS limited TSCM formation and the antiseptic effect of CHX reduced its impact on bacterial cell permeability, while Maillard's reaction produced molecules that could facilitate bacterial adhesion.
Article
Chemistry, Analytical
Behnaz Abbasgholi N. Asbaghi, Ahmed Alsadig, Loredana Casalis, Pietro Parisse, Joseph Niemela, Stefano Bellucci, Humberto Cabrera
Summary: A novel approach using a miniaturized gel electrophoresis chip integrated with online thermal lens detection was reported for monitoring the amount of DNA on the surface of gold nanoparticles, enabling the detection of small differences in DNA surface density and high sensitivity biorecognition. The system demonstrated a low detection limit and potential as an advanced instrumental platform for designing biosensors in nanotechnology.
MICROCHEMICAL JOURNAL
(2022)
Article
Biology
Jose Angel Huerta-Ocampo, Lino Gerardo Batista-Roche, Martha Beatriz Morales-Amparano, Maria del Refugio Robles-Burgueno, Gabriela Ramos-Clamont Montfort, Luz Vazquez-Moreno, Fernando Ramirez-Jimenez, Luis M. Teran
Summary: Velvet mesquite, a native legume of the southwestern United States and northwestern Mexico, plays a key role in the desert ecosystem but is also a major cause of allergic respiratory disease. This study identified allergenic proteins in velvet mesquite pollen, which will contribute to the development of specific diagnosis and treatment for mesquite pollen allergy.
Article
Chemistry, Analytical
Csenge Filep, Andras Guttman
Summary: This study utilized capillary SDS gel electrophoresis with dextran and borate cross-linking to analyze the electrophoretic process and molecular characteristics of SDS-protein complexes with the help of 3D Ferguson plots and KR plots, revealing the impact of dextran and borate concentrations on separation characteristics during electrophoresis.
ANALYTICA CHIMICA ACTA
(2021)
Article
Environmental Sciences
Nazneen Akhtar, Sehresh Khan, Muhammad Jamil, Shafiq Ur Rehman, Zia Ur Rehman, Eui Shik Rha
Summary: This study investigated the combined effect of ZnO NPs and bacteria on protein and gene expression profiles of rice plants grown in heavy metal-polluted water, showing that the combined treatment increased nitrogen-protein content and protein expression in plants.
Article
Biochemical Research Methods
Martha Beatriz Morales-Amparano, Alejandra Valenzuela-Corral, Gabriela Ramos-Clamont Montfort, Luz Vazquez-Moreno, Abraham Escobedo-Moratilla, Guillermo Pastor-Palacios, Cesare Ovando-Vazquez, Luis M. Teran, Jose Angel Huerta-Ocampo
Summary: Pecan pollen is a significant cause of allergic respiratory disease, with 17 IgE binding proteins identified in this study. These findings are crucial for the development of specific diagnosis and treatment for pecan pollen allergy.
JOURNAL OF PROTEOMICS
(2021)
Article
Microbiology
Muhammad Ali Syed, Bushra Jamil, Hazem Ramadan, Maria Rukan, Shahzad Ali, Shahid Ahmad Abbasi, Tiffanie A. Woodley, Charlene R. Jackson
Summary: The study characterized Staphylococcus aureus isolates from a tertiary care hospital in Rawalpindi, Pakistan, and found high levels of resistance, particularly for MRSA strains. The majority of S. aureus isolates belonged to specific spa types, with the most common clone identified as ST772-t657 (Bengal Bay clone).
Article
Biochemistry & Molecular Biology
Magdalena Kwolek-Mirek, Aleksandra Dubicka-Lisowska, Sabina Bednarska, Renata Zadrag-Tecza, Pawel Kaszycki
Summary: Copper-zinc superoxide dismutase (SOD1) is an antioxidant enzyme that regulates oxidative stress-related alterations in the phenotype of the yeast Saccharomyces cerevisiae. Comparative proteomic analysis of the wild-type strain and Delta sod1 mutant revealed differential expression of proteins related to carbon metabolism, amino acid and protein biosynthesis, nucleotide biosynthesis, and antioxidant processes. The study suggests that protein comparative profiling of the Delta sod1 yeast can provide valuable insights into the systemic alterations in the S. cerevisiae phenotype caused by the SOD1 mutation.
Article
Biochemical Research Methods
Jaswinder Singh, Kuldip Paliwal, Tongchuan Zhang, Jaspreet Singh, Thomas Litfin, Yaoqi Zhou
Summary: Recent advancements in computational prediction of RNA secondary structure through deep learning and transfer learning have significantly improved accuracy in canonical base-pairing structures as well as tertiary interactions such as pseudoknots and non-canonical base pairs. The new method shows high accuracy for RNAs with over 1000 homologous sequences and can also improve base-pairing prediction by incorporating artificial but functional homologous sequences generated from deep mutational scanning. This fully automatic method provides a powerful tool for capturing both secondary and tertiary base-pairing information for building three-dimensional models.
Article
Biochemistry & Molecular Biology
Maria Luisa Castrejon-Godinez, Efrain Tovar-Sanchez, Ma Laura Ortiz-Hernandez, Sergio Encarnacion-Guevara, Angel Gabriel Martinez-Batallar, Magdalena Hernandez-Ortiz, Enrique Sanchez-Salinas, Alexis Rodriguez, Patricia Mussali-Galante
Summary: Methyl parathion, a widely used pesticide, poses a significant threat to ecosystems and human health due to its high toxicity and persistence in the environment. Identifying microorganisms capable of degrading methyl parathion is crucial for bioremediation efforts at pesticide-polluted sites.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Fuqiang Ge, Qiaoling Yu, Jun Zhang, Yaoyao Han, Dongfa Zhu, Xi Xie
Summary: The E93 gene was identified and found to be widely distributed in adult tissues of the swimming crab. The expression levels of PtE93 mRNA in Y-organ and epidermis fluctuated during the molt cycle, suggesting its involvement in juvenile molting. Treatment with 20E induced PtE93 expression, while treatment with MF showed the opposite effect. The study also observed a negative correlation between PtE93 and ecdysteroid biosynthesis genes.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Manuel Marrero, Oscar Monroig, Jose A. Perez, Monica B. Betancor, Ana Galindo, Ana Bolanos, N. Guadalupe Acosta, Covadonga Rodriguez
Summary: This study investigated the combined effects of partial dietary replacement of fish oil (FO) by vegetable oil (VO) and reduced ambient salinity on the fatty acid composition of C. labrosus juveniles. The results showed that C. labrosus is able to biosynthesise EPA and DHA from ALA, and low salinity conditions can upregulate the gene expression related to fatty acid metabolism. Fish reared on FO20 diet showed the highest amount of n-3 LC-PUFA in muscle.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Davide Degli Esposti, Aureline Lalouette, Karen Gaget, Louveline Lepeule, Zineb Chaabi, Maxime Lepretre, Anabelle Espeyte, Nicolas Delorme, Herve Queau, Laura Garnero, Federica Calevro, Arnaud Chaumot, Olivier Geffard
Summary: Metal pollution has significant effects on aquatic environments, and metallothioneins play important roles in maintaining metal homeostasis and detoxification. This study identified two metallothionein-coding transcripts in Gammarus fossarum and investigated their organ-specific expression patterns. The results showed that mt1 was more strongly induced after cadmium exposure, and mt1 was more highly induced in the caeca compared to the gills for any metal exposure. Additionally, mt2 was more inducible in the gills than in the caeca for cadmium and zinc exposure at the individual level.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Cuihong You, Shuangshuang Miao, Zhiyong Xie, Siyuan Lin, Shuqi Wang, Cuiying Chen, Li Lin, Yanhua Huang, Meng Zhou, Yewei Dong, Yuanyou Li, Ping Zhuang
Summary: The rabbitfish S. guttatus was found to have the capability for LC-PUFA biosynthesis, with the expression of related genes varying temporally and spatially during embryogenesis and being influenced by ambient salinity.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Saydur Rahman, Mohammad Maruf Billah, Victor Rangel, Esmirna Cantu
Summary: Global warming has severe impacts on the reproductive fitness of marine invertebrates. In this study, the researchers used various techniques to investigate the effects of high temperatures on global DNA methylation, cellular apoptosis, and nitrative stress in the gonads of Atlantic sea urchins.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
E. S. Kladchenko, A. A. Tkachuk, M. S. Podolskaya, A. Yu. Andreyeva
Summary: This study investigated the osmotic stability and cellular responses of hemocytes in Mediterranean mussel and Pacific oyster. The results revealed that both species showed cellular volume changes and alterations in mitochondrial membrane potential upon hypoosmotic swelling. These findings provide insights into the cellular responses of bivalve mollusks to osmotic stress.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Yuya Ohhara, Chiemi Sagisaka, Kimiko Yamakawa-Kobayashi
Summary: This study investigated the biosynthetic capacity of n-3 PUFAs in the terrestrial invertebrate Sinella dubiosa, and found that it can metabolize oleic, linolenic, and arachidonic acids to produce EPA, suggesting that it may serve as an EPA source in soil ecosystems.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Correction
Biochemistry & Molecular Biology
N. Perales-Garcia, D. Tovar-Ramirez, M. G. Martinez-Morales, B. P. Ceballos-Vazquez, D. A. Corona-Rojas, M. A. Salcedo-Meza, A. Garrido-Mora, F. Vega-Villasante, H. Nolasco-Soria
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
N. Perales-Garcia, D. Tovar-Ramirez, M. G. Martinez-Morales, B. P. Ceballos-Vazquez, D. A. Corona-Rojas, M. A. Salcedo-Meza, A. Garrido-Mora, F. Vega-Villasante, H. Nolasco-Soria
Summary: This study provides the first report on the digestive tract pH of the pygmy octopus. The pH of the digestive tract regions was found to be lower than 7.0 and did not differ significantly between wild and captive octopuses. The digestive gland had the lowest pH values, but they were still above 5.0.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Carlos Espirito-Santo, Carmen Alburquerque, Francisco A. Guardiola, Rodrigo O. A. Ozorio, Leonardo J. Magnoni
Summary: Swimming has physiological effects on farmed fish, but the potential link between swimming and oxidative stress is still poorly understood. This study investigated the impact of different moderate swimming conditions on the antioxidant status of gilthead seabream, revealing that swimming modulated the activity of antioxidant enzymes in the liver and skeletal muscle. Adequate swimming conditions were found to minimize oxidative stress in gilthead seabream.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Yingxin Zhang, Chaobin Qin, Junli Wang, Liping Yang, Xiao Yan, Shaoyang Zhi, Guoxing Nie
Summary: The study focuses on the expression patterns of PFK genes in response to glucose, insulin, and glucagon stimuli in grass carp. The findings suggest that PFK family genes have different or even opposite expression patterns in various tissues, which may contribute to glucose intolerance in fish.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Jianshuang Li, Likang Lyu, Haishen Wen, Yun Li, Xiaojie Wang, Yijia Yao, Xin Qi
Summary: This study investigated the transcriptional regulation of estrogen receptors (ESRs) on isotocin receptors (itrs) in guppies, and found that estrogen can regulate oxytocin receptor expression in ovoviviparous teleosts. The study also demonstrated that ESR1 represses itr2 promoter activity in an estrogen-dependent manner, while overexpression of ESR2a or ESR2b enhances itr2 promoter activity.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Wan-Xin Li, Li-Ting Cai, Yan-Ping Huang, Yuan-Qing Huang, Shu-Hui Pan, Zhi-Long Liu, Charles Brighton Ndandala, Gang Shi, Si-Ping Deng, Hong-Juan Shi, Guang-Li Li, Dong-Neng Jiang
Summary: In this study, it was found that S. argus has two lep genes, with lepa highly expressed in the liver and lepb not detected in any tissue. lepa was found to play a role in regulating female reproduction in S. argus.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Li-Xiang Wu, Xiao-Ying Tan, Yi-Chuang Xu, Hua Zheng, Xiao-Lei Wei, Wu-Hong Lv, Zhi Luo
Summary: This study investigates the mechanisms underlying the activation of lipophagy in yellow catfish under high glucose-induced lipid deposition. The SIRT1-NRF2-TFEB pathway is proposed to mediate lipophagy activation, and lipophagy helps alleviate high glucose-induced lipid deposition. This study provides insights into the control of lipophagy in response to metabolic stress.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
