期刊
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
卷 154, 期 1, 页码 17-24出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cbpb.2009.04.014
关键词
Lipopolysaccharide and beta-1; 3-glucan binding protein; Eriocheir sinensis; Pattern recognition proteins; Haemocyte; Aeromonas hydrophila
资金
- National Nature Science Foundation of China [30771670]
- Shanghai Municipal Science and Technology Commission [06DJ14003]
- Shanghai Municipal Education Commission [E03009]
- Zhejiang Key Science and Technology Program, China [2006C12005, 2005C12006-1]
- Ministry of Science and Technology of China [2006BAD03B03]
- National Agricultural Transformation of Scientific and Technological Achievements Fund of China [2006GB2C-000088]
- Research Fund for the Doctoral Program of Higher Education
- Shanghai Municipal Education Commission
- Shanghai Education Development Foundation [06GG06]
The lipopolysaccharide and beta-1, 3-glucan binding protein (LGBP) is a pattern recognition protein which is fundamental for the innate immune response of crustaceans. A partial cDNA produced by the random sequencing of cDNA clones from a haemocyte cDNA library of Eriocheir sinensis showed similarity to the LGBP gene of the Chinese shrimp (Fenneropenaeus chinensis). The full-length cDNA was subsequently cloned and sequenced by the technique of rapid amplification of cDNA ends (RACE). The E. sinensis LGBP gene (designated as Es-LGBP) was 1236 bases long and was capable of encoding a polypeptide of 362 amino acids showing significant similarity to homologous genes in shrimp. The crab LGBP deduced amino acid sequence carrying conserved features of this gene family including a potential recognition motif for beta-1, 3 linkages of polysaccharides and putative RGD (Arg-Gly-Asp) cell adhesion sites. Real-time quantitative reverse transcription-PCR (RT-PCR) analysis showed that LGBP gene expresses in haemocytes, hepatopancreas, muscles, gills, stomachs, and intestines with the highest level in haemocytes and the lowest in the stomach. The LGBP gene expression is up-regulated upon bacterial infection and the binding of lipopolysaccharide and beta-1, 3-glucan to LGBP could induce a series of immune reactions. The temporal expression of the LGBP gene after bacterial challenge was measured by real-time quantitative RT-PCR. The result demonstrated that the LGBP gene expression in crab was up-regulated at 1.5 h post-injection of bacteria followed by a step by step recovery at 12 and 24 h. Our data suggest that the crab LGBP is an inducible acute-phase protein that could be critical in crab immunity. (C) 2009 Elsevier Inc. All rights reserved.
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