Article
Environmental Sciences
Zeyin Huang, Jiahao Gao, Yuebi Chen, Zhang Huan, Yue Liu, Tianyang Zhou, Zhongdian Dong
Summary: This study examined the effects of the environmental endocrine disruptor bisphenol AF (BPAF) on marine medaka. The results showed that BPAF exposure decreased the survival and hatchability of marine medaka embryos, caused abnormal heart rate and organ morphology, and altered the expression of functional genes.
ENVIRONMENTAL TOXICOLOGY
(2023)
Article
Environmental Sciences
Naveen Ranasinghe, Chia-Hao Lin, Tsung-Han Lee
Summary: This study revealed the relationship between cholesterol metabolism and SIRT1, LXR alpha, and SREBP-1 in fish, as well as the effects of salinity on hepatic cholesterol metabolism in euryhaline teleosts. The results showed that SREBP-1 is involved in cholesterol biosynthesis in fish, and knockdown of SREBP-1 has differential effects on the expression of key enzymes involved in cholesterol biosynthesis.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Environmental Sciences
Guozhu Ye, Xu Zhang, Xinyu Liu, Xu Liao, Han Zhang, Changzhou Yan, Yi Lin, Qiansheng Huang
Summary: The study found that exposure to polystyrene microplastics significantly altered the metabolism of marine medaka livers, leading to changes in various metabolic pathways and levels of specific metabolites. The effects of different sizes of microplastics (10-mu m and 200-mu m) on hepatic metabolism were observed, with differences in the impact on carbohydrate, amino acid, and lipid metabolism. Overall, the results provide new insights into the ecotoxicology of microplastics in aquatic organisms.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Cell Biology
Mi Ju Son, Seung Pyo Gong
Summary: In this study, we successfully established 22 embryonic cell lines with intercellular genetic homogeneity using feeder cells expressing basic fibroblast growth factor. These cell lines exhibited characteristics of fish embryonic stem cells and will contribute to the development of methods for derivation of fish ESCs.
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
(2022)
Article
Engineering, Environmental
Shu-Qing Qiu, Guo-Yong Huang, Xiao-Pei Li, Dong-Qiao Lei, Chen-Si Wang, Guang-Guo Ying
Summary: This study investigates the potential endocrine disrupting effects of plastic leachates on marine organisms, particularly on marine medaka larvae. The results show that endogenous estradiol is more sensitive to plastic leachates than other hormones, and estrogen receptor alpha is the most affected gene. Aged plastics have a greater endocrine disrupting effect compared to virgin plastics, and the additives in plastic leachates also play an important role in regulating the endocrine system.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Naveen Ranasinghe, Wei-Zhu Chen, Yau-Chung Hu, Lahiru Gamage, Tsung-Han Lee, Chuan-Wen Ho
Summary: This study investigated the metabolic molecular adjustments of ectothermic fish in response to hypothermal stress. The results showed that the branchial ATP content was significantly higher in the cold-stress group, but not in the cold-tolerance group. The expression of genes related to mitochondrial energy metabolism exhibited differential responses to cold-stress and cold-tolerance environments. The findings provide insights into the complex ways in which fish adjust their metabolism when exposed to cold stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Marine & Freshwater Biology
Shu-Qing Qiu, Guo-Yong Huang, Xiao-Pei Li, Dong-Qiao Lei, Chen-Si Wang, Guang-Guo Ying
Summary: The issue of additives leached from plastics and their endocrine disrupting effects have attracted attention. This study assessed the transcriptional responses of endocrinerelated genes in marine medaka embryos exposed to leachates from aged plastics immersed in simulated seawater or fish digest. The results showed significant differences in gene transcripts between the two types of leachates, and other leached additives may also impact gene transcription. This study provides molecular evidence of the endocrine disrupting effects of aged plastic leachates and calls for further research on the primary factors affecting transcriptional alterations.
AQUATIC TOXICOLOGY
(2023)
Article
Marine & Freshwater Biology
Pingping Liang, Hafiz Sohaib Ahmed Saqib, Zeyang Lin, Ruping Zheng, Yuting Qiu, Yuting Xie, Dongna Ma, Yingjia Shen
Summary: The study found that marine medaka's growth and tissue responses in different salinity environments are affected by salinity changes. The gills are more sensitive to extreme hypotonic environments, while the liver is more susceptible to hypertonic environments. Functional analysis of differentially expressed genes showed that gills mainly regulate ion balance and signal transduction, while the liver is involved in protein synthesis and processing.
AQUATIC TOXICOLOGY
(2021)
Article
Fisheries
Mika Watanabe, Yo Okamura, Tomoya Kono, Masahiro Sakai, Jun-ichi Hikima
Summary: This study analyzed the functional significance of IL-22 in fish gills and found that IL-22 may be involved in immune responses through the production of inflammatory cytokines and antimicrobial peptides in fish gills.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2023)
Article
Fisheries
Xiaoting Liang, Shi Duan, Qing Yang, Xiaoqin Ma, Zhenyu Li, Qiting Yao, Kongyue Wu, Pei Chang, Gongqing Feng, Wentao Hong, Mengxi Cao, Qingchun Zhou, Xueping Zhong, Haobin Zhao
Summary: Prmt5 is an enzyme that catalyzes arginine dimethylation in proteins and is conserved from yeast to humans. It is necessary for embryonic development in mice and maintains embryonic stem cells. However, the knockout of prmt5 in zebrafish does not affect the viability of the embryos, suggesting that prmt5 may be dispensable during embryogenesis in fish. In this study using medaka, a different fish organism, the mutation of prmt5 resulted in down-regulation of stemness genes and up-regulation of genes associated with cell death, indicating that prmt5 is necessary for embryogenesis in medaka by maintaining stemness and repressing apoptosis.
Article
Marine & Freshwater Biology
Zijun Ye, Liping Huang, Qianyu Zhao, Wei Zhang, Li Zhang
Summary: Using transcriptomic analysis, we identified key genes involved in AsB synthesis in marine medaka, including bhmt, mat2aa, and gstt1a. This study provides important insights into the mystery of AsB synthesis in marine fish.
AQUATIC TOXICOLOGY
(2022)
Article
Marine & Freshwater Biology
Yongmeng Yang, Aifeng Li, Jiangbing Qiu, Wenhui Yan, Lilin Han, Dongyue Li, Chao Yin
Summary: The study explored the toxicological effects of lipophilic okadaic acid (OA)-group toxins on marine fish and found increased mortality, decreased hatching rates, and various malformations in marine medaka embryos exposed to OA. It also identified significant accumulation of reactive oxygen species, increased CAT enzyme activity, and dose-dependent increase in AChE activity in 1-month old medaka larvae exposed to OA. Transcriptome analysis revealed potential DNA damage and cancer risk caused by OA, as well as neurotoxicity and risk of major depressive disorder. Further research on genotoxicity and neurotoxicity of OA to marine fish is needed.
AQUATIC TOXICOLOGY
(2023)
Article
Environmental Sciences
Mitsushi Yamamoto, Nobuhiro Kanazawa, Miho Nomura, Yoshifumi Horie, Hideo Okamura
Summary: Bisphenol A (BPA), an endocrine disruptor found in freshwater and marine environments, has been shown to induce feminization in male marine medaka fish. The expression of gonadal soma-derived factor (gsdf) decreased, while liver vitellogenin (vtg) expression increased, indicating the potential use of vtg and gsdf as biomarkers for evaluating the impact of estrogenic endocrine disruptors in O. melastigma.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Environmental Sciences
Xian Qin, Keng Po Lai, Rudolf Shiu Sun Wu, Richard Yuen Chong Kong
Summary: This study investigated the effects of continuous exposure to 17 alpha-ethinylestradiol (EE2) on reproductive performance in marine medaka fish. The results showed that continuous EE2 exposure reduced fecundity and sperm motility, and caused dysregulation in testicular transcriptome. Key genes identified in this study can serve as biomarkers for assessing EE2-induced reduction in sperm motility.
MARINE POLLUTION BULLETIN
(2022)
Article
Environmental Sciences
Jun Wang, Xuan Li, Ming Gao, Xuefu Li, Lingchao Zhao, Shaoguo Ru
Summary: This study found that polystyrene microplastics (MPs) increased the estrogenic effects of 17 alpha-ethynylestradiol (EE2) on marine fish, leading to damage in the reproductive system and suppression in growth, as well as an increase in plasma 17 beta-estradiol (E-2) levels. MPs also increased the concentration of EE2 in seawater, exacerbating its toxicity.
Article
Biochemistry & Molecular Biology
Sang Hyuck Lee, Mi Ae Kim, Young Chang Sohn
Summary: This study characterized the CCAP signaling system in Pacific abalone and revealed its relationship with the calcium/PKC and cAMP/PKA signal transduction pathways. It provides new insights into the evolutionary origins of the NPS and OT/VP signaling systems in protostomes.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Hanna Scheuffele, Erica V. Todd, John A. Donald, Timothy D. Clark
Summary: Global warming is causing an increase in extreme weather events, affecting ecosystems and the behavior of ectotherms. More research is needed to understand the impact of diurnal thermal variability and improve climate change predictions.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Jaakko Haverinen, Ahmed Badr, Markus Eskelinen, Matti Vornanen
Summary: The acclimatization of certain ectothermic vertebrates to winter conditions involves reduced energy consumption, achieved through decreased movement activity, depression of cellular functions, or switching to anaerobic energy production. This study on crucian carp showed that winter-acclimatized fish exhibited lower metabolic rates and a shift to anaerobic energy production during anoxia. Winter dormancy in crucian carp is achieved through active reduction of energy consumption, a slowed metabolic rate, and a direct response to oxygen absence.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Maria Cruz Sueiro, Cynthia A. Awruch, Gustavo M. Somoza, Walter S. Svagelj, Maria G. Palacios
Summary: According to life-history theory, there might be different immunological strategies between reproductive and non-reproductive periods due to limited resources. Our study on two sympatric marine fish species, rockfish and sandperch, found that reproductive individuals showed lower immune function, but higher levels of natural antibodies in rockfish females, compared to their non-reproductive counterparts. On the other hand, reproductively active sandperch males showed lower levels of natural antibodies and higher neutrophil to lymphocyte ratio and spleen index compared to non-reproductive males. The study highlights the species-specific patterns of immunity and the potential influence of resource limitation and abiotic factors on immune trade-offs in fish.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
James B. Cummins, Dane A. Crossley II
Summary: This study focuses on cardiovascular development in altricial bird species, which has been less studied compared to precocial species. The researchers investigated the cholinergic and adrenergic receptor mediated control of arterial blood pressure and heart rate in the neotropic cormorant. The findings suggest that both cholinergic and adrenergic tone play a role in cardiovascular regulation in embryonic birds.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Ru Xia, Hong-Kang Liu, Xi-Feng Liu, Xin Deng, Chuan-Jie Qin, Yuan-Fa He, Shi-Mei Lin, Yong-Jun Chen
Summary: This study cloned the genes encoding glucokinase and glucose-6-phosphatase catalytic subunit in largemouth bass, revealing their distribution and transcriptional regulation. The results showed that the functions of G6pc and Gck in LMB were highly conserved in evolution, and the interconversion of glucose and G6P in the liver was well regulated at the transcript level under high starch diet, but a futile cycle was induced after a glucose load.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)