Article
Clinical Neurology
K. Kmet'ova, D. Drobna, R. Liptak, J. Hodosy, P. Celec
Summary: This study describes the early dynamics of plasma GFAP and ecDNA after TBI in mice. The results suggest that plasma ecDNA is a sensitive early marker of TBI, while GFAP is not.
Article
Biochemistry & Molecular Biology
L'ubica Janovicova, Katarina Kmet'ova, Nikola Pribulova, Jakub Janko, Barbora Gromova, Roman Gardlik, Peter Celec
Summary: This study investigated the impact of high endogenous DNase activity on an animal model of acute liver failure. It was found that mice with higher DNase activity had lower mortality after 48 hours. The study also suggested that vesicles or DNA-binding proteins may play a role in protecting ecDNA from nucleases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Nutrition & Dietetics
Veronika Kovacova, Radoslav Omelka, Vladimira Mondockova, Piotr Londzin, Jozef Conka, Veronika Meliskova, Joanna Folwarczna, Peter Celec, Monika Martiniakova
Summary: Short-term animal experiments and association studies have suggested that cola intake may have a negative impact on bone mineral density (BMD). This study investigated the effects of long-term cola consumption on femoral bone microstructure in adult mice. The results showed that cola intake did not significantly affect trabecular and cortical bone parameters, although slight deterioration was observed. Cola consumption also slightly worsened bone mechanical properties, but it was not statistically significant. Long-term cola intake did not cause evident pathological changes, possibly due to a balanced diet and physical activity.
Article
Multidisciplinary Sciences
Lucia Mihalovicova, Veronika Kunstekova, David Milacek, Jakub Janko, Michal Pastorek, Barbora Konecna, Radana Gurecka, Zuzana Rausova, Olga Ulicna, Peter Celec, Katarina Sebekova
Summary: The study investigates the impact of maternal gestational diabetes (GDM) on the metabolic status of offspring. GDM was induced in CD1 mice consuming a fast-food diet (FFD) before mating. Offspring from dams consuming standard chow or FFD served as controls. Results showed that male GDM offspring exhibited growth retardation and lower kidney weight. GDM offspring, regardless of sex, had the lowest IL-1 alpha levels, while other inflammatory markers showed inconsistent changes. Offspring from dams consuming FFD had higher liver triacylglycerols content.
SCIENTIFIC REPORTS
(2023)
Article
Medicine, General & Internal
Maria Fussiova, Peter Svec, Julia Horakova, Petr Sedlacek, Peter Rohon, Peter Celec, Ivana Bod'ova, Jaroslava Adamcakova, Tomas Sykora, Veronika Dobsinska, Miroslava Pozdechova, Dominika Doczyova, Santia Vargova, Alexandra Kolenova
Summary: This study compared the course of HVOD in children before and after the implementation of new EBMT criteria. The use of EBMT criteria resulted in a higher recognition rate of anicteric disease, lower serum bilirubin levels at diagnosis, shorter treatment duration, decreased mortality, and shorter hospitalization.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Kristina Licha, Michal Pastorek, Gabriela Repiska, Peter Celec, Barbora Konecna
Summary: The study aimed to investigate the presence of ecDNA in sEVs isolated from fresh human plasma by ultracentrifugation and density gradient. It was found that approximately 60-75% of DNA is on the surface of sEVs, while a part of the DNA is localized inside the sEVs. Furthermore, both nuclear and mitochondrial DNA were present in plasma EVs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Lubica Janovicova, Katarina Kmetova, Lubomira Tothova, Barbora Vlkova, Peter Celec
Summary: Urinary DNA is studied as a non-invasive marker to monitor kidney health and urinary tract tumors. The concentration of urinary DNA, especially mitochondrial DNA, reflects kidney damage in renal diseases. Sample processing affects urinary DNA concentrations, with centrifugation at 1600 g decreasing DNA concentration by 66% in high DNA samples. Additional centrifugation at 16000 g does not significantly decrease DNA concentration in the supernatant. Deoxyribonuclease treatment reduces total and nuclear DNA, but most urinary mitochondrial DNA is protected. Understanding the biology of urinary DNA is important for clinical interpretation.
MOLECULAR AND CELLULAR PROBES
(2023)
Article
Physics, Applied
Barbora Gromova, Barbora Konecna, Maria Suchonova, Slavomir Pasztor, Peter Celec, Lubomira Tothova, Michal Lichvar, Jaroslav Budis, Jan Radvanszky, Diana Rusnakova, Tomas Szemes, Zdenko Machala, Roman Gardlik
Summary: This study analyzed the impact of plasma-activated water (PAW) on oxidative status and gut microbiota diversity in healthy mice and mice with inflammatory bowel disease (IBD). The results showed that PAW increased oxidative stress markers in the colon tissue, especially in IBD mice. Interestingly, PAW increased microbial diversity in a healthy gut but decreased it in an inflamed gut. The findings suggest that PAW may not have therapeutic potential in treating IBD.
PLASMA PROCESSES AND POLYMERS
(2023)
Article
Pediatrics
Katarina Krivosikova, Nadja Supcikova, Alexandra Gaal Kovalcikova, Jakub Janko, Michal Pastorek, Peter Celec, Ludmila Podracka, Lubomira Tothova
Summary: This study aimed to investigate the role of NET-associated markers in children with UTI and the role of NETs formation in a mouse model of UTI. The results showed that children with UTI had significantly higher urinary NETs markers including ecDNA, MPO, and cathelicidin, which were positively correlated with leukocyturia. The experimental model also revealed that mice with diminished NETs formation had a higher bacterial load in the bladder and kidneys, suggesting that NETs may play a direct role in preventing pyelonephritis and other UTI complications.
FRONTIERS IN PEDIATRICS
(2023)
Article
Oncology
Peter Lesko, Barbora Vlkova, Katarina Kalavska, Valentina De Angelis, Vera Novotna, Jana Obertova, Zuzana Orszaghova, Patrik Palacka, Katarina Rejlekova, Zuzana Sycova-Mila, Boris Kollarik, Ramadan Aziri, Daniel Pindak, Jozef Mardiak, Michal Chovanec, Peter Celec, Michal Mego
Summary: This study aims to analyze the association between plasma vitamin D and clinico-pathological findings and prognosis in patients with germ-cell tumors (GCTs). The results showed that low plasma vitamin D levels were associated with an unfavorable response to therapy and disease recurrence. However, it is still unknown whether low vitamin D has a causative role in the disease and whether its supplementation affects the outcome.
FRONTIERS IN ONCOLOGY
(2023)
Article
Oncology
Michal Chovanec, Katarina Kalavska, Jana Obertova, Patrik Palacka, Katarina Rejlekova, Zuzana Sycova-Mila, Zuzana Orszaghova, Peter Lesko, Valentina De Angelis, Lucia Vasilkova, Daniela Svetlovska, Beata Mladosievicova, Jozef Mardiak, Michal Pastorek, Barbora Vlkova, Peter Celec, Michal Mego
Summary: This study found that disruption of the intestinal barrier may contribute to cognitive impairment in survivors of testicular germ cell tumors through the gut-blood-brain axis. Higher levels of plasma sCD14 were associated with worse cognitive function in these patients. These findings provide new insights into understanding and addressing cognitive impairment in survivors of testicular germ cell tumors.
FRONTIERS IN ONCOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Michaela Mihal'ova, Nadja Supcikova, Alexandra Gaal Kovalcikova, Jan Breza Jr, L'ubomira Tothova, Peter Celec, Jan Breza Sr
Summary: Extracellular DNA (ecDNA) in the plasma and urine of urinary tract infection (UTI) patients was studied to understand its dynamics. The study found that urinary ecDNA levels decreased significantly during hospitalization and remained low during follow-up. Plasma ecDNA levels did not change, but plasma DNase activity increased during follow-up. C-reactive protein (CRP) and procalcitonin levels in plasma and urine were positively correlated with ecDNA, and urinary ecDNA levels were also correlated with urinary bacterial burden in a mouse UTI model.
Article
Biochemistry & Molecular Biology
Kristina Macakova, Petra Psenkova, Nadja Supcikova, Barbora Vlkova, Peter Celec, Jozef Zahumensky
Summary: Infection with SARS-CoV-2 during pregnancy and vaccination against COVID-19 do not appear to cause major disturbances in the balance between free radical production and antioxidant activity in the placenta, as indicated by biomarkers of oxidative damage and antioxidant capacity. There were no significant differences in extracellular DNA (ecDNA) levels or deoxyribonuclease activity in the umbilical cord plasma among the study groups. These findings suggest that the risk of oxidative stress in the placenta is not significantly affected by SARS-CoV-2 infection or COVID-19 vaccination during pregnancy.
Article
Pediatrics
Katarina Sebekova, Radana Gurecka, Melinda Csongova, Ivana Koborova, Peter Celec
Summary: Cardiometabolic risk factors in young age are linked to a higher risk of developing atherosclerotic cardiovascular disease later in life. Atherogenic dyslipidemia is closely associated with obesity and metabolic syndrome in young individuals. It is uncertain whether cardiometabolic risk factors are related to the atherogenic index of plasma (AIP = log (TAG/HDL-C) in lean individuals with low atherogenic risk. Both AIP and markers of cardiometabolic risk are continuous variables, leading us to hypothesize that their association would be linear before the onset of obesity and atherogenic dyslipidemia.
Meeting Abstract
Biotechnology & Applied Microbiology
P. Belvoncikova, V. Borbelyova, B. Gromova, B. Schuh, R. Gardlik, P. Celec
HUMAN GENE THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Sang Hyuck Lee, Mi Ae Kim, Young Chang Sohn
Summary: This study characterized the CCAP signaling system in Pacific abalone and revealed its relationship with the calcium/PKC and cAMP/PKA signal transduction pathways. It provides new insights into the evolutionary origins of the NPS and OT/VP signaling systems in protostomes.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Hanna Scheuffele, Erica V. Todd, John A. Donald, Timothy D. Clark
Summary: Global warming is causing an increase in extreme weather events, affecting ecosystems and the behavior of ectotherms. More research is needed to understand the impact of diurnal thermal variability and improve climate change predictions.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Jaakko Haverinen, Ahmed Badr, Markus Eskelinen, Matti Vornanen
Summary: The acclimatization of certain ectothermic vertebrates to winter conditions involves reduced energy consumption, achieved through decreased movement activity, depression of cellular functions, or switching to anaerobic energy production. This study on crucian carp showed that winter-acclimatized fish exhibited lower metabolic rates and a shift to anaerobic energy production during anoxia. Winter dormancy in crucian carp is achieved through active reduction of energy consumption, a slowed metabolic rate, and a direct response to oxygen absence.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Maria Cruz Sueiro, Cynthia A. Awruch, Gustavo M. Somoza, Walter S. Svagelj, Maria G. Palacios
Summary: According to life-history theory, there might be different immunological strategies between reproductive and non-reproductive periods due to limited resources. Our study on two sympatric marine fish species, rockfish and sandperch, found that reproductive individuals showed lower immune function, but higher levels of natural antibodies in rockfish females, compared to their non-reproductive counterparts. On the other hand, reproductively active sandperch males showed lower levels of natural antibodies and higher neutrophil to lymphocyte ratio and spleen index compared to non-reproductive males. The study highlights the species-specific patterns of immunity and the potential influence of resource limitation and abiotic factors on immune trade-offs in fish.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
James B. Cummins, Dane A. Crossley II
Summary: This study focuses on cardiovascular development in altricial bird species, which has been less studied compared to precocial species. The researchers investigated the cholinergic and adrenergic receptor mediated control of arterial blood pressure and heart rate in the neotropic cormorant. The findings suggest that both cholinergic and adrenergic tone play a role in cardiovascular regulation in embryonic birds.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Ru Xia, Hong-Kang Liu, Xi-Feng Liu, Xin Deng, Chuan-Jie Qin, Yuan-Fa He, Shi-Mei Lin, Yong-Jun Chen
Summary: This study cloned the genes encoding glucokinase and glucose-6-phosphatase catalytic subunit in largemouth bass, revealing their distribution and transcriptional regulation. The results showed that the functions of G6pc and Gck in LMB were highly conserved in evolution, and the interconversion of glucose and G6P in the liver was well regulated at the transcript level under high starch diet, but a futile cycle was induced after a glucose load.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)
