期刊
JOURNAL OF MEDICINAL CHEMISTRY
卷 58, 期 18, 页码 7549-7556出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jmedchem.5b01147
关键词
-
资金
- [25860022]
- Grants-in-Aid for Scientific Research [25860022] Funding Source: KAKEN
Farnsyl pyrophosphate synthase (FPPS) catalyzes the condensation of isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate to FPP and is known to be a molecular target of osteoporosis drugs, such as risedronate (RIS), which is a nitrogen-containing bisphosphonate. The protonation states and hydration structure of RIS bound to FPPS were determined by neutron protein crystallography, which allows direct visualization of hydrogens and deuteriums. The structure analysis revealed that the phosphate groups of RIS were fully deprotonated with the abnormally decreased plc, and that the roles of E93 and D264 consisted of canceling the extra negative charges upon the binding of ligands. Collectively, our neutron structures provided insights into the physicochemical properties during the bisphosphonate binding event.
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