期刊
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
卷 162, 期 1, 页码 36-43出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cbpa.2012.02.006
关键词
Cytochrome P4501A; Endocrine disruption; Hormone receptor; Metallothionein; Vitellogenin
资金
- National Natural Science Foundation of China [NSFC 20977092, 40821003, U1133005, 40688001]
- Guangdong Provincial Natural Science Foundation [8251064004000001]
- China Postdoctoral Science Foundation [20100480786]
To assess the adverse toxicological effects of steroid hormones on western mosquitofish (Gambusia affinis), 180 adult females were exposed to individual or binary combinations of progesterone (1 mu g/L), testosterone (1 mu g/L) and 17 beta-estradiol (1 mu g/L) for eight days. The expression patterns of vitellogenin, estrogen receptor, androgen receptor, metallothionein, and cytochrome P450 1A genes in mosquitofish varied according to tissue as well as the specificity of steroids. Treatment by progesterone or testosterone alone inhibited target gene expression in the livers. The expression levels of both vitellogenin A and vitellogenin B mRNAs were up-regulated by17 beta-estradiol, and a parallel induction of estrogen receptor a mRNA expression was also observed in the livers. In addition, 17 beta-estradiol treatment alone suppressed androgen receptor a, metallothionein and cytochrome P450 1A mRNA expression in the livers. In general, multiple hormone treatments had different effects on target gene expression compared with corresponding hormone alone. The results demonstrate that steroid hormones cause multiple biological responses including the expression of vitellogenin, estrogen receptor and androgen receptor mRNA in the hormone signaling pathways and the expression of metallothionein and cytochrome P450 1A mRNA in the xenobiotic signaling pathway. (C) 2012 Elsevier Inc. All rights reserved.
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