期刊
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
卷 155, 期 3, 页码 275-280出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cbpa.2009.11.016
关键词
Mitochondrion-rich cell; Chloride cell; Pavement cell; Gill bar; Opercular epithelium; Primary cell culture; CFTR; NKCC; V-type H+-ATPase
资金
- NSERC Canada
This short review traces the history of in vitro experimental methods that have been used to help elucidate the ion transport mechanisms of teleost fish gills. It begins with an isolated gill preparation published by Denis Bellamy in 1961 and progresses through many different approaches and concludes with current techniques. Among them are perfused gill arches, primary cultures of gill epithelia, isolated opercular skin preparations, whole embryos in vitro, the yolk-ball technique, dissociated gill epithelial cells, vibrating microprobe and scanning ion-selective microelectrodes; currently all are combined with molecular biological techniques. Each new approach brought new findings but is subject to certain limitations and each has contributed significantly to this important subfield of comparative physiology. (C) 2009 Elsevier Inc. All rights reserved.
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