期刊
JOURNAL OF MEDICAL VIROLOGY
卷 88, 期 4, 页码 664-673出版社
WILEY
DOI: 10.1002/jmv.24368
关键词
hepatitis B virus (HBV); genotype; mutations; a determinant; EPI
类别
资金
- National Research University Project, Office of Higher Education Commission [WCU001-HR-57, WCU007-HR-57, WCU-58-006-HR]
- National Research Council of Thailand (NRCT)
- Research Chair Grant from the National Science and Technology Development Agency, Chulalongkorn University Centenary Academic Development Project [CU56-HR01]
- Ratchadaphiseksomphot Endowment Fund of Chulalongkorn University [RES560530093]
- Outstanding Professor of Thailand Research Fund [DPG5480002]
- Siam Cement Group
- MK Restaurant Company Limited
Hepatitis B virus (HBV) infection affects an estimated two billion people worldwide. Since 1992, Thailand implemented universal HBV vaccination as part of the expanded program on immunization (EPI) for newborns. This study aims to compare genotypes and characterize HBV by assessing pre-S/S and basic core promoter (BCP)/precore (PC) mutations in populations born before and after EPI implementation. A nationwide serosurvey conducted in 2014 assessed the impact of universal HBV vaccination in Thailand. Two cohort groups were established based on whether they were born before or after 1992. HBV DNA was amplified from HBsAg positive samples by PCR and sequenced. HBV genotypes, pre-S/S regions, and BCP/PC mutations were characterized. From a total of 5,964 subjects, there were 2,805 (47.0%) and 3,159 (53.0%) individuals who were born before and after EPI implementation, respectively. The overall prevalence of HBsAg was 2.2%. The prevalence of HBsAg was significantly higher in the before EPI group (4.3%) than in the after EPI group (0.3%) (P<0.001). HBV DNA was detected in 119 samples; 111 HBV-positive samples (93%) were genotype C (subgenotype C1). The a determinant mutation was only detected in the before EPI group. Twenty-two years after implementation of the EPI program, the HBV carrier rate is significantly reduced. The most prevalent genotype for the remaining HBV was C1. The vaccine escape mutant, especially the a determinant, was not detected after the launch of the EPI program, and the current HBV vaccine remains highly effective. J. Med. Virol. 88:664-673, 2016. (c) 2015 Wiley Periodicals, Inc.
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