Article
Immunology
Chiara Mazziotta, Carmen Lanzillotti, Elena Torreggiani, Lucia Oton-Gonzalez, Maria Rosa Iaquinta, Elisa Mazzoni, Pauline Gaboriaud, Antoine Touze, Ettore Silvagni, Marcello Govoni, Fernanda Martini, Mauro Tognon, John Charles Rotondo
Summary: The study utilized a novel indirect ELISA method using two synthetic peptides to analyze antibodies against MCPyV. Results showed high sensitivity, specificity, and accuracy in detecting IgGs against MCPyV VP mimotopes, indicating a relatively high prevalence of MCPyV infection among the population.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Asta Luciunaite, Indre Dalgediene, Emilija Vasiliunaite, Milda Norkiene, Indre Kucinskaite-Kodze, Aurelija Zvirbliene, Alma Gedvilaite
Summary: Polyomaviruses are common in humans and animals, and some of them may have potential zoonotic transmission. This study investigated the immunogenic properties of VP1 virus-like particles derived from human polyomaviruses (HPyVs). The results showed that these VP1 particles are highly immunogenic and have antigenic similarities among different polyomaviruses. The study also revealed the interaction between VP1 particles and phagocytes. This research provides important insights into the study of polyomavirus biology and its interaction with the host immune system using VP1 particles.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, General & Internal
Yu Zuo, Sherwin Navaz, Wenying Liang, Chun Li, Colby R. Ayers, Christine E. Rysenga, Alyssa Harbaugh, Gary L. Norman, E. Blair Solow, Bonnie Bermas, Oludamilola Akinmolayemi, Anand Rohatgi, David R. Karp, Jason S. Knight, James A. de Lemos
Summary: This population-based cohort study found that the prevalence of antiphospholipid antibodies (aPL) tested at a single time point was 14.5%, with approximately one-third at a moderate or high titer. The IgA isotypes of anticardiolipin and anti-beta-2 glycoprotein I were associated with future atherosclerotic cardiovascular disease (ASCVD) events.
Article
Immunology
Stanley A. Plotkin
Summary: Vaccine development has evolved from a trial-and-error approach to a more scientific approach, utilizing antigenic characterization, structural biology, immune function definition, protein synthesis, and precise definition of immune correlates of protection to address the increasingly challenging biology of current targets.
CLINICAL INFECTIOUS DISEASES
(2022)
Review
Hematology
Massimo Radin, Irene Cecchi, Marta Arbrile, Davide Montin, Loredana Farinasso, Michele Cioffi, Silvia Grazietta Foddai, Alice Barinotti, Elisa Menegatti, Simone Baldovino, Savino Sciascia, Dario Roccatello
Summary: The aim of this study was to investigate the epidemiology, clinical and laboratory characteristics of pediatric antiphospholipid syndrome (APS) by conducting a literature review and a registry-based study in Northwest Italy. The literature review included six articles with a total of 386 pediatric patients, while the registry-based study included 17 pediatric patients from the Northwest Italy. The results showed that pediatric APS had severe clinical manifestations with a high prevalence of noncriteria manifestations.
SEMINARS IN THROMBOSIS AND HEMOSTASIS
(2023)
Article
Multidisciplinary Sciences
Meige Liu, Gongming Li, Xiaodong Song, Yangyi Fan, Chun Li
Summary: This study investigated the prevalence and risk factors of central nervous system (CNS) manifestations in antiphospholipid syndrome (APS) and evaluated stroke recurrence. The results showed that older age, livedo reticularis, and dyslipidemia were significant factors related to CNS manifestations. The use of anticoagulants and/or hydroxychloroquine appeared to be associated with a lower incidence of CNS manifestations. Livedo reticularis, smoking, and male gender may predict the risk of recurrent stroke in APS patients.
SCIENTIFIC REPORTS
(2023)
Article
Medicine, General & Internal
Danielle Brazel, Priyanka Kumar, Hung Doan, Tianyu Pan, Weining Shen, Ling Gao, Justin T. Moyers
Summary: Merkel cell carcinoma (MCC) is a rare and aggressive skin cancer with increasing incidence. This study aimed to identify actionable alterations associated with tumor mutation burden (TMB) using the OncoKB database. The findings suggest that targeted therapies may be a viable treatment option for selected MCC patients.
Article
Allergy
Jun Tanaka, Yuma Fukutomi, Yoshiki Shiraishi, Asako Kitahara, Tsuyoshi Oguma, Yuto Hamada, Kentaro Watai, Tadashi Nagai, Masami Taniguchi, Koichiro Asano
Summary: This study found a high prevalence of allergen-specific IgE antibodies to inhaled allergens among healthy adults in Japan, with regional variations.
ALLERGOLOGY INTERNATIONAL
(2022)
Article
Virology
Maria Dolci, Lucia Signorini, Wafa Toumi, Giuseppe Basile, Sarah D'Alessandro, Pasquale Ferrante, Serena Delbue
Summary: This study found the presence of HPyVs in colon cancer patients, with a higher positivity rate in tumor tissues, indicating a potentially significant role of MCPyV in colon tumorigenesis.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Review
Oncology
Steffi Silling, Alexander Kreuter, Thilo Gambichler, Thomas Meyer, Eggert Stockfleth, Ulrike Wieland
Summary: Merkel cell polyomavirus (MCPyV) is a common virus on human skin, leading to the development of a rare but aggressive Merkel cell carcinoma (MCC) in older individuals, especially those with fair skin, male sex, and immunosuppression. The incidence of MCC, caused by MCPyV or UV damage, is increasing globally, with risk factors including male sex, older age, fair skin, intense UV exposure, and immunosuppression. Early diagnosis and prompt treatment are crucial for reducing MCC morbidity and mortality.
Article
Rheumatology
Ofir Aluf, Silvia Parente, Massimo Radin, Loretta Campa, Valentina Gazzola, Akindamola Olugbogi, Nicolo Tesio, Heinrich Ugo, Edoardo Zanetti, Carla Zevallos, Elisabetta Versino, David Lembo, Dario Roccatello, Savino Sciascia
Summary: This comprehensive systematic analysis of studies investigating the prevalence of aPL in renal TMA showed that, despite the high heterogeneity of the included studies, aPL are present in about one case out of four renal-TMA cases.
Article
Medicine, General & Internal
Luis Miguel Osorio-Toro, Diana Marcela Bonilla-Bonilla, Jorge Enrique Daza-Arana, Juan Carlos Aristizabal, Juan Carlos Avila-Valencia, Roberto Ramirez-Marmolejo
Summary: This study aims to determine the seroprevalence of COVID-19 among healthcare workers and describe the associated sociodemographic and labor features. The results show an adjusted seroprevalence of 29% in health workers and factors related to seropositivity include having a contact with a COVID-19 patient for >120 minutes and being diagnosed with COVID-19 by laboratory tests.
INTERNATIONAL JOURNAL OF GENERAL MEDICINE
(2023)
Article
Medicine, Research & Experimental
Monique E. Verhaegen, Paul W. Harms, Julia J. Van Goor, Jacob Arche, Matthew T. Patrick, Dawn Wilbert, Haley Zabawa, Marina Grachtchouk, Chia-Jen Liu, Kevin Hu, Michael C. Kelly, Ping Chen, Thomas L. Saunders, Stephan Weidinger, Li-Jyun Syu, John S. Runge, Johann E. Gudjonsson, Sunny Y. Wong, Isaac Brownell, Marcin Cieslik, Aaron M. Udager, Arul M. Chinnaiyan, Lam C. Tsoi, Andrzej A. Dlugosz
Summary: Merkel cell carcinoma (MCC) is an aggressive skin cancer that expresses specific genes similar to skin-resident Merkel cells. Researchers have used ATOH1 to induce MCC development in mice by cellular reprogramming. By conditionally expressing MCPyV TAgs and ATOH1 in mouse epidermal cells, they were able to generate MCC-like tumor cells from hair follicles. The study confirmed the similarity between mouse and human MCCs and revealed that loss of p53 is necessary for the progression of MCC in this mouse model.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Review
Medicine, General & Internal
Rifqah Abeeda Roomaney, Brian van Wyk, Eunice Bolanle Turawa, Victoria Pillay-van Wyk
Summary: The prevalence of multimorbidity in South Africa varies from low to moderate (3%-23%) in studies including younger populations to moderate to high (30%-87%) in studies of older adults. Hypertension is identified as the main driver of multimorbidity in the South African population, especially among older adults. Further research on multimorbidity in South Africa should focus on high-quality study designs to better understand this issue.
Article
Psychiatry
William W. Eaton, Katrina M. Rodriguez, Mekha A. Thomas, Jeanette Johnson, Monica V. Talor, Curtis Dohan, Clifton O. Bingham III, Rashelle Musci, Kimberly Roth, Deanna L. Kelly, Daniela Cihakova, Erika Darrah
Summary: The relationship between schizophrenia and rheumatoid arthritis has been observed for 85 years, but the mechanisms driving this association are unknown. This study analyzed differences in cytokines, selected genes, and antibodies related to gluten sensitivity, celiac disease, and systemic autoimmunity in patients with schizophrenia, rheumatoid arthritis, and healthy controls. The analysis revealed potential subgroups within schizophrenia patients based on their cytokine profiles, providing new directions for treatment strategies.
PSYCHIATRY RESEARCH
(2022)
Article
Virology
Martina Salakova, Eva Koslabova, Zuzana Vojtechova, Ruth Tachezy, Vojtech Sroller
JOURNAL OF MEDICAL VIROLOGY
(2016)
Article
Immunology
S. Nemeckova, V. Sroller, M. Stastna-Markova
TRANSPLANT INFECTIOUS DISEASE
(2016)
Article
Cell & Tissue Engineering
Pavlina Ptackova, Jan Musil, Martin Stach, Petr Lesny, Sarka Nemeckova, Vlastimil Kral, Milan Fabry, Pavel Otahal
Article
Virology
Barbora Pokryvkova, Martina Salakova, Jana Smahelova, Zuzana Vojtechova, Vendula Novosadova, Ruth Tachezy
Article
Cell & Tissue Engineering
Martin Stach, Pavlina Ptackova, Martin Mucha, Jan Musil, Pavel Klener, Pavel Otahal
Article
Biochemistry & Molecular Biology
Jaroslav Nunvar, Lucie Pagacova, Zuzana Vojtechova, Nayara Trevisan Doimo de Azevedo, Jana Smahelova, Martina Salakova, Ruth Tachezy
Summary: Our study found that the degree of miRNA deregulation varies significantly among tumors at different locations, and the deregulated miRNAs are largely tumor-specific, with no miRNA shared by all four types of HPV-positive tumors.
Article
Biochemical Research Methods
Sarka Vanikova, Abhishek Koladiya, Jan Musil
Summary: This 29-color panel is developed and optimized for monitoring NK cell and T cell reconstitution in patients' peripheral blood post-HSCT, considering major complications and relevant lymphocyte populations. It covers all major NK cell and T cell subsets, focusing on markers and properties of CD57 + NKG2C+ cells, as well as characterization of T cell reconstitution, including detection of CD4+ RTEs. Overall, the panel allows comprehensive analysis of the reconstituting immune system and identification of potential markers of immune cell dysfunction.
Article
Immunology
Marketa Stastna-Markova, Eva Hamsikova, Petr Hainz, Petr Hubacek, Marie Kroutilova, Jitka Krystofova, Viera Ludvikova, Jan Musil, Pavla Pecherkova, Martina Salakova, Vojtech Sroller, Jan Vydra, Sarka Nemeckova
Summary: Measurement of pretransplant anti-BKPyV antibodies and T-cell immunity can predict the risk of BKPyV-related complications, such as hemorrhagic cystitis, in HSCT recipients. Early identification of high-risk patients can be achieved through a combination of clinical and immunological pretransplant risk factors.
Article
Biochemistry & Molecular Biology
Jan Louzil, Jana Stikarova, Dana Provaznikova, Ingrid Hrachovinova, Tereza Fenclova, Jan Musil, Martin Radek, Jirina Kaufmanova, Vera Geierova, Eliska Ceznerova, Peter Salaj, Roman Kotlin
Summary: A single-center study on patients with inherited disorders of primary hemostasis found that most patients presented with bleeding symptoms but had no definitive diagnosis. By implementing advanced diagnostic methods, such as next-generation sequencing (NGS), the diagnostic rate has been improved.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Jana Mackova, Petr Hainz, Jitka Krystofova, Katerina Roubalova, Marketa Stastna-Markova, Sarka Vanikova, Jan Musil, Jan Vydra, Sarka Nemeckova
Summary: The study investigated the effects of immunosuppressive medication and immune system reconstitution on T cell responses to COVID-19 vaccine in allo-HSCT recipients. The results showed that corticosteroid therapy significantly suppressed the immune response to the vaccine in HSCT recipients. The timing of vaccination was also significantly associated with the vaccine response. Well-reconstituted CD4+ T cells at six months after HSCT were important for vaccine efficacy.
Article
Oncology
Martin Stach, Robert Pytlik, Kristyna Smilauerova, Jana Rychla, Martin Mucha, Jan Musil, Abhishek Koladiya, Matej Nemec, Martina Petrackova, Iva Kastankova, Pavla Pecherkova, Lucie Sramkova, Kamila Polgarova, Marek Trneny, Petr Lesny, Jan Vydra, Pavel Otahal
Summary: This study analyzed the memory phenotype and expression of inhibitory receptors of CAR-T cells in patients who received intensive pretreatment. The results suggest that the level of expansion of CAR-T cells in vivo is an important parameter for predicting therapy efficacy, rather than the immunophenotype.
PATHOLOGY & ONCOLOGY RESEARCH
(2023)
Meeting Abstract
Immunology
M. Kroutilova, J. Musil, Alexova K. Zurkova, P. Gabriel, J. Mackova, S. Nemeckova
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)
Meeting Abstract
Hematology
Martin Stach, Jan Musil, Petr Cetkovsky, Pavel Otahal
Article
Immunology
Eva Hamsikova, Jana Smahelova, Viera Ludvikova, Martina Salakova, Jana Rychla, Jana Skrenkova, Lukas Rob, Ruth Tachezy