☆ 4.3 Article

Added value of multi-pathogen probe-based real-time FOR SeptiFast in the rapid diagnosis of bloodstream infections in patients with bacteraemia

JOURNAL OF MEDICAL MICROBIOLOGY (2015)

期刊

JOURNAL OF MEDICAL MICROBIOLOGY

卷 64, 期 -, 页码 670-675

出版社

MICROBIOLOGY SOC

DOI: 10.1099/jmm.0.000074

关键词

-

类别

Microbiology

向作者/读者索取更多资源

Protocol

Reagent

摘要

The commercial multi-pathogen probe-based real-time FOR SeptiFast (SF) was evaluated as a rapid and complementing tool for the microbiological diagnosis of bloodstream infections (BSIs) in a series of 138 matched blood samples from 65 patients with bacteraemia, hospitalized in an intensive care unit, when antibiotics had already been administered. SF was positive in 32.6% of the samples, whereas blood culture (BC) was positive in 21.7% (P<0.05). SF identified more pathogens (11 versus 5; specificity, 90.7%) and reduced the time of aetiological diagnosis, with a mean of 16.3 versus 55.4 h needed for BC (P<0.05). SF enabled appropriate pathogen-oriented therapy in 72% (36/50) of the BSI group of patients on the basis of epidemiological data. According to our data, the use of SF provided important added value to BC, in terms of earlier aetiological diagnosis of BSIs, enabling pathogen-oriented therapy in patients receiving empirical antibiotic treatment.

作者

我是这篇论文的作者

点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.3

评分不足

次要评分

新颖性

-

重要性

-

科学严谨性

-

评价这篇论文

推荐

Article Multidisciplinary Sciences

Metabolic preference assay for rapid diagnosis of bloodstream infections

Thomas Rydzak, Ryan A. Groves, Ruichuan Zhang, Raied Aburashed, Rajnigandha Pushpker, Maryam Mapar, Ian A. Lewis

Summary: Bloodstream infections cause a significant number of infections and deaths annually, and delayed administration of appropriate antimicrobials increases mortality rates. A rapid metabolic preference assay using metabolic flux patterns can efficiently identify pathogens and determine their antimicrobial susceptibility, reducing testing time to less than 20 hours.

NATURE COMMUNICATIONS (2022)

添加到收藏夹

Review Infectious Diseases

Diagnosis of Bloodstream Infections: An Evolution of Technologies towards Accurate and Rapid Identification and Antibiotic Susceptibility Testing

Kristel C. Tjandra, Nikhil Ram-Mohan, Ryuichiro Abe, Marjan M. Hashemi, Jyong-Huei Lee, Siew Mei Chin, Manuel A. Roshardt, Joseph C. Liao, Pak Kin Wong, Samuel Yang

Summary: Bloodstream infections are a major cause of death worldwide, and the lack of timely and reliable diagnostic practices is an ongoing issue. The current blood culture practice is time-consuming and cannot provide timely results. Therefore, there is an urgent need for new technologies that can offer accurate and timely diagnosis and susceptibility testing.

ANTIBIOTICS-BASEL (2022)

添加到收藏夹

Article Chemistry, Analytical

A Rapid Single-Cell Antimicrobial Susceptibility Testing Workflow for Bloodstream Infections

Britney Forsyth, Peter Torab, Jyong-Huei Lee, Tyler Malcom, Tza-Huei Wang, Joseph C. Liao, Samuel Yang, Erik Kvam, Chris Puleo, Pak Kin Wong

Summary: Bloodstream infections are a significant global health concern, and rapid initiation of effective antibiotic treatment is crucial. A culture-free workflow for the rapid isolation and enrichment of bacterial pathogens from whole blood facilitates single-cell antimicrobial susceptibility testing, leading to faster diagnosis and treatment.

BIOSENSORS-BASEL (2021)

添加到收藏夹

Review Immunology

Diagnosis and Management of Bloodstream Infections With Rapid, Multiplexed Molecular Assays

Sherry A. Dunbar, Christopher Gardner, Shubhagata Das

Summary: This article describes the application of two rapid, molecular bloodstream infection panels that can identify the most common pathogens and associated genetic determinants of antibiotic resistance, providing clinicians with faster information for treatment. Molecular testing methods reduce the turnaround time for diagnosing bloodstream pathogens to a few hours, helping to initiate timely and effective treatment.

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY (2022)

添加到收藏夹

Article Medical Laboratory Technology

Clinical Evaluation of an Improved Metagenomic Next-Generation Sequencing Test for the Diagnosis of Bloodstream Infections

Chendi Jing, Hongbin Chen, Yong Liang, Ying Zhong, Qi Wang, Lifeng Li, Shijun Sun, Yifan Guo, Ruobing Wang, Zhi Jiang, Hui Wang

Summary: The mNGS workflow showed a high clinical sensitivity of 87.1%, improved clinical specificity of 80.2%, and reduced false-positive results by utilizing multiple control groups. This optimization can enhance the utility of mNGS as a powerful tool for identifying pathogens in bloodstream infections.

CLINICAL CHEMISTRY (2021)

添加到收藏夹

Article Critical Care Medicine

Epidemiology, Resistance Profiles, and Outcomes of Bloodstream Infections in Community-Onset Sepsis in the United States*

Tetsu Ohnuma, Shingo Chihara, Blair Costin, Miriam Treggiari, Raquel R. Bartz, Karthik Raghunathan, Vijay Krishnamoorthy

Summary: In patients with community-onset sepsis, the prevalence of blood culture-positive sepsis was 14%, and resistant organisms were infrequent. Blood culture-positive sepsis and nonblood culture-positive sepsis were associated with lower in-hospital mortality compared to culture-negative sepsis.

CRITICAL CARE MEDICINE (2023)

添加到收藏夹

Review Infectious Diseases

Advances in rapid diagnostics for bloodstream infections

Neima Briggs, Sheldon Campbell, Shaili Gupta

Summary: Septicemia from bloodstream infections (BSI) is a significant cause of inpatient mortality and a costly condition to manage for US hospitals. Advances in diagnostic systems have accelerated the identification and antimicrobial susceptibility testing of causative pathogens, but clinical impact remains varied due to the need for conventional AST and prescriber understanding. Rapid diagnostic platforms show improved outcomes when used in conjunction with active antimicrobial stewardship, and newer technologies are expanding on rapid detection of genotypic determinants of resistance.

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE (2021)

添加到收藏夹

Article Oncology

A practical update on the epidemiology and risk factors for the emergence and mortality of bloodstream infections from real-world data of 3014 hematological malignancy patients receiving chemotherapy

Shaozhen Chen, Kangni Lin, Qian Li, Xiaofeng Luo, Min Xiao, Minmin Chen, Haojie Zhu, Yongquan Chen, Xueqiong Wu, Yanling Zeng, Yuxin Zhang, Issa Hajji Ally, Jingjing Xu, Jinhua Ren, Zhizhe Chen, Jianda Hu, Ting Yang

Summary: This study focused on bloodstream infections (BSI) in patients with hematologic malignancies (HM) receiving chemotherapy, finding a high BSI rate primarily caused by Gram-negative bacteria, especially multidrug-resistant (MDR) strains. Various factors like gender, age, hospital stays, neutropenia, antibiotic use, and infection sites were found to predict BSI emergence, while factors like age, neutropenia, HM remission status, white blood cell count, antibiotic use, infection sites, and specific bacterial strains predicted 30-day mortality. High attention should be paid to managing these risk factors for effective clinical care.

JOURNAL OF CANCER (2021)

添加到收藏夹

Review Geriatrics & Gerontology

Bloodstream infections in the elderly: what is the real goal?

Yaara Leibovici-Weissman, Noam Tau, Dafna Yahav

Summary: Bloodstream infections (BSI) in older patients have higher incidence and mortality rates compared to younger patients, with potential differences in disease presentation. Old patients with BSI commonly have sources from urinary and respiratory tracts, with gram-negative bacteria being the most common isolates and long-term care facilities serving as reservoirs for multidrug-resistant bacteria. Mortality rates for BSI in older patients are higher, associated with factors such as appropriateness of antibiotic therapy and nosocomial acquisition of infection, while health-related quality of life issues for elderly patients with BSI are not well addressed in the literature.

AGING CLINICAL AND EXPERIMENTAL RESEARCH (2021)

添加到收藏夹

Article Microbiology

Rapid Identification of Pathogens Causing Bloodstream Infections by Raman Spectroscopy and Raman Tweezers

Katarina Rebrosova, Silvie Bernatova, Martin Siler, Jan Masek, Ota Samek, Jan Jezek, Martin Kizovsky, Veronika Hola, Pavel Zemanek, Filip Ruzicka

Summary: Bloodstream infections are a common cause of hospitalizations and are often life-threatening. Timely identification of the causative agent and its antimicrobial susceptibility and resistance profiles is essential for effective therapy. Raman spectroscopy is a potential method that meets the criteria of being reliable, accurate, low-cost, real-time, and easy-to-use.

MICROBIOLOGY SPECTRUM (2023)

添加到收藏夹

Article Chemistry, Analytical

Nanoparticles-based Culture-free method for rapid and sensitive detection of Staphylococcus aureus in bloodstream infections

Yuting Wang, Jingsong Xu, Li Cao, Shuang Yang, Lina Zhao, Lei He, Zhen Shen, Weiwei Lin, Bishen Shi, Qian Liu, Zhi Huang, Min Li, Hua Wang

Summary: Staphylococcus aureus is a versatile and virulent pathogen. A nanoparticle-based culture-free method using vancomycin modified magnetic nanoparticles and droplet digital PCR was developed to rapidly concentrate and detect S. aureus in blood within 3 hours, significantly shortening the detection time compared to traditional methods. The limit of detection was as low as 10 CFU/mL, improving the diagnostic rate of bacteremia. This new method could be a promising tool for early identification and treatment guidance of S. aureus bloodstream infections, thereby reducing mortality.

MICROCHEMICAL JOURNAL (2023)

添加到收藏夹

Article Biochemistry & Molecular Biology

Phenotypic and Genotypic Virulence Characterisation of Staphylococcus pettenkoferi Strains Isolated from Human Bloodstream and Diabetic Foot Infections

Chloe Magnan, Nour Ahmad-Mansour, Cassandra Pouget, Madjid Morsli, Sylvaine Huc-Brandt, Alix Pantel, Catherine Dunyach-Remy, Albert Sotto, Virginie Molle, Jean-Philippe Lavigne

Summary: This study performs whole-genome analysis on S. pettenkoferi strains and investigates their phylogenetic relationships, resistome, and virulome. Additionally, the study explores their ability to form biofilm, growth kinetics, and virulence in an in vivo zebrafish embryo infection model. The findings reveal genetic differences among strains and diversity in biofilm formation types.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022)

添加到收藏夹

Article Chemistry, Multidisciplinary

A dual-caged resorufin probe for rapid screening of infections resistant to lactam antibiotics

Jinghang Xie, Ran Mu, Mingxi Fang, Yunfeng Cheng, Fiona Senchyna, Angel Moreno, Niaz Banaei, Jianghong Rao

Summary: This study presents a rapid screening assay for pathogenic bacteria resistant to lactam antibiotics, utilizing a fluorogenic probe that shows superior sensitivity in reporting the activity of beta-lactamases and allows for detection of resistant bacteria in urine samples within 2 hours.

CHEMICAL SCIENCE (2021)

添加到收藏夹

Article Infectious Diseases

Impact of rapid susceptibility testing on antimicrobial therapy and clinical outcomes in Gram-negative bloodstream infections

Vanesa Anton-Vazquez, Cristina Suarez, Timothy Planche

Summary: This study evaluated the impact of rapid antimicrobial susceptibility testing on antimicrobial therapy and clinical outcomes in patients with Gram-negative bloodstream infection. The results showed that rapid susceptibility testing led to faster discontinuation of aminoglycosides and a shorter time to starting effective and optimal antibiotic therapy.

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY (2022)

添加到收藏夹

Article Microbiology

Comparison of three rapid diagnostic tests for bloodstream infections using Benefit-risk Evaluation Framework (BED-FRAME)

Richard D. Smith, Min Zhan, Shanshan Zhang, Surbhi Leekha, Anthony Harris, Yohei Doi, Scott Evans, J. Kristie Johnson, Robert K. Ernst

Summary: This study evaluated a new rapid lipid analysis technique called FLAT MS for direct identification of pathogens from blood cultures. FLAT MS showed slight differences in accuracy compared to other FDA-approved methods for identifying Gram-positive bacteria, Gram-negative bacteria, and yeast. The time-to-results and hands-on time of FLAT MS were comparable to other methods.

JOURNAL OF CLINICAL MICROBIOLOGY (2023)

添加到收藏夹

Article Biochemistry & Molecular Biology

Different Modulatory Effects of Four Methicillin-Resistant Staphylococcus aureus Clones on MG-63 Osteoblast-Like Cells

Nicolo Musso, Giuseppe Caruso, Dafne Bongiorno, Margherita Grasso, Dalida A. Bivona, Floriana Campanile, Filippo Caraci, Stefania Stefani

Summary: In this study, different Staphylococcus aureus strains were tested for their ability to modulate cell viability in osteoblast-like cells. Results showed that certain strains were able to internalize and persist in the cells, causing a decrease in cell viability. Additionally, differences in intracellular behavior and impact on cell functions related to inflammation, metabolism, and oxidative stress were observed among the tested strains.

BIOMOLECULES (2021)

添加到收藏夹

Article Microbiology

Genomic Characterization of a New Biofilm-Forming and Adhesive ST398 Human-Adapted MSSA Lineage Causing Septic Knee Arthritis Following Surgical Reconstruction

Viviana Cafiso, Flavia Lo Verde, Alessandra Zega, Giuseppe Pigola, Roberto Rostagno, Silvio Borre, Stefania Stefani

Summary: This study characterized two biofilm-producing M1 and M2 ST398 MSSA strains causing septic knee arthritis, revealing a new human-adapted lineage with unique antibiotic resistance and adhesive properties. The strains, susceptible to certain antibiotics but resistant to others, exhibited a high ability to adhere to the host's extracellular matrix, potentially causing septic knee arthritis.

MICROORGANISMS (2021)

添加到收藏夹

Review Microbiology

Staphylococcus aureus Internalization in Osteoblast Cells: Mechanisms, Interactions and Biochemical Processes. What Did We Learn from Experimental Models?

Stefano Stracquadanio, Nicolo Musso, Angelita Costantino, Lorenzo Mattia Lazzaro, Stefania Stefani, Dafne Bongiorno

Summary: Bacterial internalization is a strategy used by non-intracellular microorganisms to evade the host immune system and survive in the human body. Staphylococcus aureus interacts with and infects osteoblasts, leading to osteomyelitis and infections. The MG-63 osteoblastic cell line is the best traditional model for studying these interactions, although more representative 3D models have also been investigated.

PATHOGENS (2021)

添加到收藏夹

Article Microbiology

Staphylococcus aureus ST228 and ST239 as models for expression studies of diverse markers during osteoblast infection and persistence

Dafne Bongiorno, Nicolo Musso, Giuseppe Caruso, Lorenzo Mattia Lazzaro, Filippo Caraci, Stefania Stefani, Floriana Campanile

Summary: Comparative analysis of the invasion and persistence of different clinical strains of S. aureus in human MG-63 osteoblasts revealed varying levels of impact on cellular viability, suggesting the importance of considering strain-specific interactions for the development of effective treatment strategies for bone infections.

MICROBIOLOGYOPEN (2021)

添加到收藏夹

Article Virology

Discriminatory Weight of SNPs in Spike SARS-CoV-2 Variants: A Technically Rapid, Unambiguous, and Bioinformatically Validated Laboratory Approach

Nicolo Musso, Paolo Giuseppe Bonacci, Dafne Bongiorno, Stefano Stracquadanio, Dalida Angela Bivona, Concetta Ilenia Palermo, Guido Scalia, Marco Fichera, Stefania Stefani

Summary: This study describes a rapid, easy, adaptable, and affordable workflow to uniquely identify all currently known variants of SARS-CoV-2.

VIRUSES-BASEL (2022)

添加到收藏夹

Article Infectious Diseases

Heteroaryl-Ethylenes as New Effective Agents for High Priority Gram-Positive and Gram-Negative Bacterial Clinical Isolates

Dalida Angela Bivona, Alessia Mirabile, Carmelo Bonomo, Paolo Giuseppe Bonacci, Stefano Stracquadanio, Andrea Marino, Floriana Campanile, Carmela Bonaccorso, Cosimo Gianluca Fortuna, Stefania Stefani, Nicolo Musso, Dafne Bongiorno

Summary: This study evaluates the activity of a compound called PB4 on ESKAPE isolates from nosocomial infections, showing promising minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values. PB4 exhibits good antibacterial capability against various Gram-positive and Gram-negative bacteria.

ANTIBIOTICS-BASEL (2022)

添加到收藏夹

Article Chemistry, Medicinal

Elexacaftor-Tezacaftor-Ivacaftor as a Final Frontier in the Treatment of Cystic Fibrosis: Definition of the Clinical and Microbiological Implications in a Case-Control Study

Giuseppe Migliorisi, Mirella Collura, Francesca Ficili, Tiziana Pensabene, Dafne Bongiorno, Antonina Collura, Francesca Di Bernardo, Stefania Stefani

Summary: This study reports on a one-year case-control study involving 26 CF patients with at least one F508de1 mutation. The triple combination therapy targeting CFTR provided significant clinical benefits and improved patients' quality of life. Further investigations are needed to fully understand the microbiological respiratory implications and long-term advantages of elexacaftor-tezacaftor-ivacaftor treatment.

PHARMACEUTICALS (2022)

添加到收藏夹

Article Oncology

Genomic Landscape Alterations in Primary Tumor and Matched Lymph Node Metastasis in Hormone-Naive Prostate Cancer Patients

Giorgio Ivan Russo, Paolo Bonacci, Dalida Bivona, Grete Francesca Privitera, Giuseppe Broggi, Rosario Caltabiano, Jessica Vella, Arturo Lo Giudice, Maria Giovanna Asmundo, Sebastiano Cimino, Giuseppe Morgia, Stefania Stefani, Nicolo Musso

Summary: The aim of this study is to investigate genetic changes associated with lymph node metastasis in prostate cancer patients. The findings show mostly concordance between primary tumor samples and matched lymph node metastasis in terms of genomic alteration.

CANCERS (2022)

添加到收藏夹

Article Microbiology

Acinetobacter baumannii and Cefiderocol, between Cidality and Adaptability

Stefano Stracquadanio, Carmelo Bonomo, Andrea Marino, Dafne Bongiorno, Grete Francesca Privitera, Dalida Angela Bivona, Alessia Mirabile, Paolo Giuseppe Bonacci, Stefania Stefani

Summary: This study investigates the interactions between a novel cephalosporin called cefiderocol (FDC) and different strains of Acinetobacter baumannii with varying susceptibilities to the drug. The results show diverse susceptibility profiles and the possibility of non-susceptible phenotypes at high drug concentrations. Combination therapy could potentially eliminate drug-adapted subpopulations and restore full activity of FDC.

MICROBIOLOGY SPECTRUM (2022)

添加到收藏夹

Article Microbiology

Streptococcus salivarius 24SMBc Genome Analysis Reveals New Biosynthetic Gene Clusters Involved in Antimicrobial Effects on Streptococcus pneumoniae and Streptococcus pyogenes

Gaia Vertillo Aluisio, Ambra Spitale, Luca Bonifacio, Grete Francesca Privitera, Aldo Stivala, Stefania Stefani, Maria Santagati

Summary: This study characterized the oral probiotic Streptococcus salivarius 24SMBc through whole genome sequencing and annotation. The results revealed the presence of novel biosynthetic gene clusters and demonstrated its antagonistic activity against Streptococcus pneumoniae. The findings further support the use of 24SMBc in clinical settings for upper respiratory tract infections.

MICROORGANISMS (2022)

添加到收藏夹

Article Infectious Diseases

Balancing the Virulence and Antimicrobial Resistance in VISA DAP-R CA-MRSA Superbug

Rossella Salemi, Alessandra Zega, Elvira Aguglia, Flavia Lo Verde, Giuseppe Pigola, Stefania Stefani, Viviana Cafiso

Summary: This study investigated the genomic and transcriptomic adaptations of a DAP-R CA-MRSA strain that emerged in a hospitalized patient under glycopeptide treatment. The results revealed that this strain had additional antimicrobial resistance and decreased virulence compared to other CA-MRSA strains, suggesting a balance between extensive AMR and virulence in the hypervirulent genomic background.

ANTIBIOTICS-BASEL (2022)

添加到收藏夹

Article Infectious Diseases

New Antimicrobial Resistance Strategies: An Adaptive Resistance Network Conferring Reduced Glycopeptide Susceptibility in VISA

Elvira Aguglia, Eleonora Chines, Stefania Stefani, Viviana Cafiso

Summary: This study compares VISA CA-MRSA with its VSSA CA-MRSA parent in a hospitalized patient undergoing glycopeptide treatment and identifies mutational and transcriptomic adaptations in genes involved in the biosynthesis of the glycopeptide target and pathways supporting glycopeptide resistance. These adaptations include key genes responsible for the biosynthesis of peptidoglycan precursors, as well as accessory genes involved in transport and transcription.

ANTIBIOTICS-BASEL (2023)

添加到收藏夹

Review Infectious Diseases

Ceftazidime/Avibactam and Meropenem/Vaborbactam for the Management of Enterobacterales Infections: A Narrative Review, Clinical Considerations, and Expert Opinion

Andrea Marino, Edoardo Campanella, Stefano Stracquadanio, Maddalena Calvo, Giuseppe Migliorisi, Alice Nicolosi, Federica Cosentino, Stefano Marletta, Serena Spampinato, Pamela Prestifilippo, Stefania Stefani, Bruno Cacopardo, Giuseppe Nunnari

Summary: This comprehensive review examines the unique attributes, distinctions, and clinical implications of ceftazidime-avibactam (CAZ-AVI) and meropenem-vaborbactam (MEM-VAB) against difficult-to-treat Enterobacterales infections. The review provides valuable insights for healthcare professionals to make informed treatment decisions when combating carbapenem-resistant pathogens.

ANTIBIOTICS-BASEL (2023)

添加到收藏夹

Article Infectious Diseases

Ceftazidime-Avibactam Treatment for Klebsiella pneumoniae Bacteremia in Preterm Infants in NICU: A Clinical Experience

Andrea Marino, Sarah Pulvirenti, Edoardo Campanella, Stefano Stracquadanio, Manuela Ceccarelli, Cristina Micali, Lucia Gabriella Tina, Giovanna Di Dio, Stefania Stefani, Bruno Cacopardo, Giuseppe Nunnari

Summary: This study aimed to assess the safety and efficacy of Ceftazidime/avibactam (CAZ/AVI) in preterm infants admitted to the Neonatal Intensive Care Unit (NICU). Retrospective data analysis showed that all cases of Klebsiella pneumoniae bacteremia in preterm infants improved clinically and achieved microbiological cure with CAZ/AVI treatment. No adverse drug reactions were reported. Larger trials are needed to further investigate the safety and efficacy of CAZ/AVI in neonates.

ANTIBIOTICS-BASEL (2023)

添加到收藏夹

Article Microbiology

Role of transcriptomic and genomic analyses in improving the comprehension of cefiderocol activity in Acinetobacter baumannii

Stefano Stracquadanio, Alice Nicolosi, Grete Francesca Privitera, Mariacristina Massimino, Andrea Marino, Dafne Bongiorno, Stefania Stefani

Summary: The mechanisms of action and resistance of cefiderocol (FDC) in Acinetobacter baumannii are not fully understood. This study investigates the genomic and transcriptomic features of different A. baumannii strains with varying FDC susceptibility to determine the role of iron transport systems in FDC uptake. Gene expression analysis reveals up-regulation of acinetobactin and baumannoferrin genes under iron depletion and down-regulation under high iron concentrations. Overall, FDC activates the TonB energy transduction system and baumannoferrin is more stimulated by FDC in an iron-depleted medium.

MSPHERE (2023)

添加到收藏夹

暂无数据

© Peeref 2019-2024. All rights reserved.