期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 113, 期 -, 页码 134-145出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2013.08.030
关键词
Antimicrobial; Bioactivity; Cytocompatibility; Micro/nanostructure; Titania; Silver
资金
- National Basic Research Program of China (973 Program) [2012CB933600]
- National Natural Science Foundation of China [31100675, 51071168, 81271704]
- Shanghai Science and Technology RD Fund [11JC1413700]
To improve the antimicrobial ability and cytocompatibility of biomedical titanium implants, many efforts have been made to modify their surface topography and chemical composition. In this work, Ag plasma-modified hierarchical TiO2 film was fabricated on titanium surface via acid etching to produce micropit, hydrothermal treatment to generate TiO2 nanorod and subsequent plasma immersion ion implantation process to impregnate Ag into TiO2 surface. In view of the potential clinical applications, their antimicrobial activity, bioactivity and cytocompatibility were systematically evaluated. The hierarchical TiO2 film showed enhanced bioactivity and bacteriostatic effect on both microbes due to more negative zeta potential, constructing the first defense line against microbial adhesion by electrostatic repulsion. Addition of embedded Ag remarkably enhanced the antimicrobial efficiency toward both microbes based on Schottky contact without Ag+ release, establishing the second defense line targeting microbial membrane. Furthermore, the addition of Ag degraded the bioactivity very little and exerted nearly no adverse or even promoted effect on MG63 cell functions, including adhesion, spreading and proliferation. This work illustrates a two-defense-line antimicrobial activity in darkness with both prior electrostatic repulsion to inhibit most microbes adhesion and posterior biocidal action to kill residual ones that luckily infiltrated through the first defense line, and provide proof of concept using both clinically relevant human pathogens. In conclusion, the Ag-embedded hierarchical TiO2 film with excellent antimicrobial activity, bioactivity and cytocompatibility provides a promising candidate for orthopedic and dental implants. (C) 2013 Elsevier B.V. All rights reserved.
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