期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 111, 期 -, 页码 107-116出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2013.05.037
关键词
Nano-hydroxyapatite; Peptide; Polydopamine; Bioactivity; Osteogenic differentiation
资金
- Beijing Natural Science Foundation [7132124]
- State Key Development Program for Basic Research of China [2007CB936103]
- Fundamental Research Funds for the Central Universities
- Peking University's 985 Grant
To be better used as implant materials in bone graft substitutes, bioactivity and osteogenesis of nanohydroxyapatite (nano-HA) need to be further enhanced. Inspired by adhesive proteins in mussels, here we developed a novel bone forming peptide decorated nano-HA material. In this study, nano-HA was coated by one-step pH-induced polymerization of dopamine, and then the peptide was grafted onto polydopamine (pDA) coated nano-HA (HA-pDA) through catechol chemistry. Our results demonstrated that the peptide-conjugated nano-HA crystals could induce the adhesion and proliferation of MG-63 cells. Moreover, the highly alkaline phosphatase activity of the functionalized nano-HA indicated that the grafted peptide could maintain its biological activity after immobilization onto the surface of HA-pDA, especially at the concentration of 100 mu g/mL. These modified nano-HA crystals with better bioactivity and osteogenic differentiation hold great potential to be applied as bioactive materials in bone repairing, bone regeneration and bio-implant coating applications. (C) 2013 Elsevier B.V. All rights reserved.
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