Article
Spectroscopy
Ying Bao, Kexin Han, Zhifang Ding, Yue Li, Ting Li, Ming Guan, Guixin Li
Summary: Polypyrrole-luminol-AuNPs nanocomposites were prepared and utilized for a label-free electrochemiluminescence (ECL) immunosensor for detecting carbohydrate antigen 153 (CA153). The nanocomposites exhibited excellent ECL activity and good film-forming property, allowing them to be fixed on electrode surface without the need for additional film-forming materials.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Chemistry, Analytical
Lingya Ning, Yumin Bai, Zixiao Wang, Wenjun Wen, Junping Wang
Summary: A novel and rapid electro-chemiluminescence (ECL) immunosensor was successfully fabricated for ultrasensitive detection of 3-NT. The sensor utilized gold nanoparticles as the luminophore and electropolymerized polyaniline to promote the luminescence of the nanoparticles. It exhibited good analytical performance for 3-NT detection with a wide linear detection range and low detection limit, and had high recovery rate in practical sample applications.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Omer Saltuk Bolukbasi, Bahar Bankoglu Yola, Ceren Karaman, Necip Atar, Mehmet Lutfi Yola
Summary: This study successfully developed an electrochemical AFP immunosensor based on nanomaterials, which demonstrated high sensitivity and detection limit. The results showed that this sensor can be effectively used for cancer diagnosis.
Article
Chemistry, Analytical
Xinming Qin, Yu Pan, Jiachang Zhang, Jingru Shen, Chunya Li
Summary: An imidazolium based ionic liquid was prepared and used as an organic ligand for trapezoidal metal-organic frameworks (Zn-MOF) nanosheets. Gold nanoparticles (AuNPs) were integrated onto the surface of Zn-MOF nanosheets to create AuNPs@Zn-MOF nanocomposites. The resulting nanocomposites showed enhanced photocurrent response and were used as a platform for fabricating an immunosensor for alpha-fetoprotein (AFP). The photocurrent response of the immunosensor exhibited a linear relationship with the logarithm of AFP concentration, with a detection limit of 1.88 pg/mL. The photoelectrochemical immunosensor demonstrated excellent selectivity, sensitivity, stability, and reproducibility for AFP determination.
Article
Chemistry, Multidisciplinary
Haiyun Chen, Jie Huang, Rongjing Zhang, Fei Yan
Summary: In this study, the highly sensitive detection of alphafetoprotein (AFP) was achieved using electrochemiluminescence and electrochemistry. The utilization of vertically ordered mesoporous silica films as a sensing interface resulted in a signal amplification effect and excellent sensitivity. The dual-mode detection of AFP showed a low limit of detection and a wide linear range, with satisfactory results obtained in the analysis of human serum.
FRONTIERS IN CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Shyang Pei Hong, Syazana Abdullah Lim, Natasha Ann Keasberry, Minhaz Uddin Ahmed
Summary: This work presents the development of an electrochemiluminescence immunosensor using a novel nanostructured composite of titanium dioxide and gold nanourchins for the detection of protein allergen Ovomucoid found in eggs. The sensor exhibited a low limit of detection, wide linear range, and excellent selectivity, reproducibility, and recovery rates in real food samples.
BIOELECTROCHEMISTRY
(2022)
Article
Chemistry, Analytical
Wuyi Zhang, Dong Han, Zejia Wu, Kui Yang, Shiguo Sun, Jia Wen
Summary: In this study, the authors designed a hybrid material AuNPs@Fe-Zr-MOL consisting of gold nanoparticles (AuNPs) and metal-organic layers (MOLs) for the amplification of electrochemiluminescence (ECL) signal. The addition of Fe-Zr-MOL, a novel metal-organic framework with peroxidase-like activity, effectively enhanced the ECL signal of luminol. The planar structure of Fe-Zr-MOL exposed more active sites for luminol catalysis, improving its conductivity. Furthermore, the integration of AuNPs not only improved electron transport efficiency but also allowed for antibody conjugation, enabling the construction of a sandwich immuno-sensor for alpha fetoprotein (AFP) detection. The results demonstrated the effective amplification of ECL signal and immunodetection of AFP.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Tamara Guerrero-Esteban, Borja L. Sanchez, Lucia Exposito, David Rodriguez-San-Miguel, Felix Zamora, Felix Pariente, Cristina Gutierrez-Sanchez, Encarnacion Lorenzo
Summary: This work presents a novel approach for detecting biogenic amine tyramine using a sensitive and disposable electrochemiluminescent sensor. The sensor is fabricated by modifying a screen-printed carbon electrode surface with two nanomaterials, alpha-Ge nanolayers and AuNP, which synergistically enhance the electrochemiluminescence response. The sensor was characterized using various techniques such as SEM-EDX, EIS, Raman, and AFM. The principle of the biosensor relays on the fact that tyramine molecule acts as an analyte and co-reactant, which interacts with the luminophore [Ru(bpy)(3)](2+) on the sensor surface. The proposed sensor shows a linear response to tyramine concentration, with a detection limit of 2.28 mu M. The sensor successfully detected tyramine in avocado samples, demonstrating its potential for practical applications.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Haolin Xiao, Shanshan Wei, Miao Gu, Zhencheng Chen, Liangli Cao
Summary: In this study, a highly sensitive electrochemical immunosensor was developed for the effective detection of the cancer marker AFP. The platform modified with Thi and AuNPs, along with the signal probe CMK-3@AuPtNPs, resulted in improved sensitivity and signal amplification. The immunosensor demonstrated a wide linear range, low detection limit, excellent stability, and recovery, showcasing its potential for clinical applications.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Analytical
Xuwen Gao, Xiaoxuan Ren, Yaojia Ai, Mengwei Li, Bin Zhang, Guizheng Zou
Summary: This study proposes a coreactant-free electrochemiluminescence method using mercaptopropionic acid and hexametaphosphate as the capping agents. The method achieves low-triggering-potential and coreactant-free electrochemiluminescence. The method is used for the sensitive and selective determination of molecular protein ProGRP.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Zhen Wang, Jun Tu, Pei Dong, Yani Bai, Jing Han, Gang Xie
Summary: In this work, copper ion-centered hybrid nanoflowers are demonstrated to act as a high-performance redox indicator. By adjusting the concentration of bovine serum albumin (BSA) during coprecipitation, the synthesis and electrochemical properties of BSA-Cu-3(PO4)(2) HNFs can be regulated. The HNFs show great potential as nanocarriers for loading detection antibodies and can be used for label-free electrochemical immunoassays, improving the accuracy and stability of the immunosensor.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Shyang Pei Hong, Natasha Ann Keasberry, Minhaz Uddin Ahmed
Summary: In this study, a screen-printed gold electrode modified with a novel nanocomposite was used to detect Gliadin using electrochemiluminescence. The nanocomposite enhanced the intensity of electrochemiluminescence by 5.6 times compared to the bare gold electrode. The immunosensor showed a linear detection range of 0.005-500 pg/mL, with the lowest limit of detection among other Gliadin immunosensors. It exhibited high selectivity, reproducibility, stability, and excellent recoveries from real samples.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Dexin Du, Jue Wang, Mingquan Guo, Jiangnan Shu, Wei Nie, Zhiping Bian, Di Yang, Hua Cui
Summary: Label-free electrochemiluminescence (ECL) immunoassays (lf-ECLIA) have become an attractive method for biomarker detection due to their simplicity, speed, and cost-effectiveness. This study investigated the interaction between biomarkers and analytical interfaces constructed using a nanoluminophore and antibody-functionalized gold nanoparticles. The results showed that positively charged biomarkers increased the ECL intensity, while negatively charged biomarkers decreased the ECL intensity. A charge-dependent lf-ECLIA method was established based on the ECL signal changes, which exhibited different responses to positively charged and negatively charged biomarkers.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Mengyuan Wang, Huixiao Li, Yadi Wang, Xiaodie Hu, Shujun Fang, Huili Ma, Wei Li, Dianlei Wang, Zhili Han
Summary: In this study, gold nanoclusters (AuNCs) were combined with a soft chitosan (CS) nanogel matrix (AuNCs@CS) using a simple one-pot approach. The resulting AuNCs@CS nanogels showed a 2.4-fold enhancement in electrochemiluminescence (ECL) intensity compared to bare AuNCs. This enhancement was attributed to increased Au(0) content, the confinement effect of the chitosan nanogels, and the prolonged excited state lifetime of AuNCs. The AuNCs@CS nanogels were used to develop a label-free ECL immunosensor for ultrasensitive detection of aflatoxin B1 (AFB1) with a significantly lower detection limit compared to previous ECL biosensors.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Hayati Filik, Asiye Aslihan Avan, Nilay Altas Puntar, Mustafa Ozyurek, Zeynep Banu Gungor, Mine Kucur, Handan Kamis, Diclehan Ali Dicle
Summary: A sensitive sandwich-model immunoassay was designed for simultaneous electrochemical measurement of alphafetoprotein and carcinoembryonic antigen using multiple-label strategy. The immunoassay showed good linear relationship and low detection limits for both analytes. The sandwich-type immunosensor was successfully applied to real serum sample analysis.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2021)
Article
Biophysics
Cristina Potrich, Anna Pedrotti, Cecilia Pederzolli, Lorenzo Lunelli
Summary: This study developed different functional surfaces for capturing exosomes and verified their effectiveness through experiments. Among them, the negatively-charged surface performed the best, capturing a large number of exosomes and successfully analyzing their biomarkers.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2024)
Article
Biophysics
Brojendra Nath Saren, Srushti Mahajan, Mayur Aalhate, Rahul Kumar, Essha Chatterjee, Indrani Maji, Ujala Gupta, Santosh Kumar Guru, Pankaj Kumar Singh
Summary: This study developed P-selectin-targeted dasatinib nanoparticles coated with chitosan and fucoidan (DST-CH-FUC-NPs), which showed sustained release, reduced hemolytic potential, increased cytotoxicity and cellular uptake compared to free dasatinib. These nanoparticles also demonstrated enhanced ROS production, mitochondrial membrane potential damage, apoptosis induction, cell migration inhibition, and disruption of lysosomal membrane integrity.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2024)
Article
Biophysics
Weiping Deng, Xiaohui Li, Ya Li, Zhongbing Huang, Yulin Wang, Ning Mu, Juan Wang, Tunan Chen, Ximing Pu, Guangfu Yin, Hua Feng
Summary: This study demonstrates the importance of chirality in nerve repair by constructing a GO-phenylalanine derivative hydrogel system. In vivo experiments show that the dextro group significantly improves functional recovery and histological restoration in rat sciatic nerve repair models. The doped GO promotes angiogenesis and myelination. These results suggest that chirality plays a crucial role in promoting nerve regeneration.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2024)
Article
Biophysics
Xiaoyan Wang, Jin Gao, Yu Gao, Linlin Zhang, Congchao Xu, Qintong Li, Lin Li, Jianliang Xue
Summary: In this study, a highly effective surfactant producer strain, Bacillus Cereus GX7, was isolated from the oil tank bottom sludge of Shengli Oil Field in China. The biosurfactant produced by GX7 was identified as surfactin, a lipopeptide surfactant, through TLC, FT-IR, and LC-MS/MS analysis. The fermentation process of GX7 was optimized using single-factor experiments, focusing on the composition of fermentation medium and fermentation conditions. Glucose and peptone were found to be the best carbon and nitrogen sources, and the optimum temperature, inoculum amount, pH, rotation speed, and fermentation time for the strain were determined to be 30°C, 1%, 7.5, 150 rpm, and 48 h, respectively. After optimization, the surface tension and emulsification index of the fermentation broth were 26.84 mN/m and 57.84%, respectively. Furthermore, the biosurfactant produced by GX7 demonstrated good stability over a wide range of temperature, pH, and salt concentration.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2024)
Article
Biophysics
Rodney Marcelo do Nascimento, Christine Grauby-Heywang, Houssem Kahli, Nesrine Debez, Laure Beven, Ivan Helmuth Bechtold, Touria Cohen Bouhacina
Summary: This article presents a theoretical model based on thermodynamic rules to assess the early stages of bacterial biofilm formation on different material surfaces. By utilizing morphological characteristics of bacteria and Atomic Force Microscopy images, the model generates a dataset of energetically minimized states, which can be correlated with bacterial adhesion states.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2024)
Article
Biophysics
Filipa A. L. S. Silva, Licinia Timochenco, Raquel Costa-Almeida, Jose Ramiro Fernandes, Susana G. Santos, Fernao D. Magalhaes, Artur M. Pinto
Summary: The study demonstrates that by photoreducing nanosized graphene oxide using ultraviolet radiation, nanometric particles with high light-to-heat conversion efficiency and water stability can be obtained. These nanomaterials exhibit high absorption in the near-infrared region and show no cytotoxicity towards human cells, indicating their potential for safe therapy.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2024)
Article
Biophysics
D. Costa, V. De Matteis, F. Treso, G. Montani, M. Martino, R. Rinaldi, M. Corrado, M. Cascione
Summary: This review primarily discusses the relationship between contact lens discomfort (CLD) and the surface properties of contact lenses (CLs), specifically the coefficient of friction (CoF). The review emphasizes the importance of introducing a standardized protocol for measuring CoF and calls for a more precise evaluation of the relationship between surface properties and comfort in CLs users.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2024)
Article
Biophysics
Debdip Brahma, Tamal Sarkar, Rupal Kaushik, Akshay Narayan Sarangi, Amar Nath Gupta
Summary: This in-vitro study evaluates the effect of reactive oxygen species (ROS) on the structural rearrangement of elastin. The results show that oxidative stress leads to a decrease in protein size and changes in secondary structure, potentially promoting protein aggregation. This study is important for therapeutics aiming to prevent elastin degradation and aging.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2024)
Article
Biophysics
Xin Yang, Qiang Wang, Chaoxi Yan, Degang Huang, Yinchang Zhang, Huazheng He, Shouliang Xiong, Congming Li, Pingbo Chen, Tingjun Ye, Dan Hu, Lei Wang
Summary: This study presents a practical and effective strategy to improve osseointegration in patients with osteoporosis. By coating titanium implants with polydopamine followed by strontium modification, the bi-functional implants promote bone regeneration and immune regulation. The results show good biocompatibility, sustained release of strontium ions, and stable osseointegration between bone tissues and implants.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2024)
Article
Biophysics
Sengwang Fu, Jianping Zhu, Zhijun Jiang, Yue Cao, Yufei Chen, Lihao Zhang, Sunlong Li, Weipeng Lu, Chengbin Miao, Qing He, Qi Li, Weixing Zhang, Lehao Ren, Yachun Li, Hongchao Shi, Cihui Liu
Summary: Effective management of biofluids is crucial for in vivo surgical interventions. Recent advances include self-sealing needles, drug-eluting stents, and shear-thinning hydrogels. However, complications associated with intestinal mucosal injury and secondary damage still persist. In this study, researchers developed an interpenetrating Janus wettability stent coating that enables unidirectional draining of excessive biofluid. They also demonstrated directional biofluid movement using a self-pumping dressing with potential applications in biofluid collection and disease diagnosis through metal ion detection. This integrated system presents an opportunity for designing wound dressings with effective biofluid management and metal ion detection capabilities.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2024)
Article
Biophysics
Maryam Hassani, Mojtaba Kamankesh, Mazda Rad-Malekshahi, Kobra Rostamizadeh, Farhad Rezaee, Ismaeil Haririan, Seyed Mojtaba Daghighi
Summary: Bacterial adhesion and biofilm formation on the surface of biomaterial implants is a critical problem, and a polymer brush coating with antiadhesive and antimicrobial properties has proven to be highly effective in resolving this issue.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2024)
Article
Biophysics
Huaihong Zhang, Na Liu, Yuting Zhang, Hui Cang, Zhaosheng Cai, Ziqun Huang, Jun Li
Summary: A functionalized cationic polymer, CR-PQAC, was designed and synthesized for photothermal enhanced antimicrobial therapy. The CR-PQAC nanoparticles exhibited significant antibacterial activity and low cytotoxicity against mammalian cells.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2024)
Article
Biophysics
Ipsita Sahu, Priyadarshi Chakraborty
Summary: Peptide nanotechnology bridges the gap between materials and biological worlds by utilizing self-assembly of short-peptide building blocks. Hydrogels engineered from these short peptides show great potential in biomedical applications, but their weak mechanical properties and limited functional diversity need to be addressed. Nanoengineering the networks of these hydrogels by incorporating small molecules, polymers, and inorganic/carbon nanomaterials can enhance their mechanical properties and expand their functional diversity.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2024)
Article
Biophysics
Hao Wei, Yajun Gu, Ao Li, Panpan Song, Dingding Liu, Feihu Sun, Xiaofeng Ma, Xiaoyun Qian
Summary: In this study, a stable three-dimensional conductive hydrogel was prepared by cross-linking MXenes to Matrigel hydrogel. The conductive hydrogel promotes the proliferation and differentiation of NSCs, providing new strategies for neural tissue engineering.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2024)
Article
Biophysics
Yue Zhong, Xiaoli Ge, Juan Zhang, Qun Wei, Feng Wang, Yongke Zhong
Summary: The effect of oxygenic groups on the hemocompatibility of activated carbon was studied through liquid-phase oxidation and subsequent heat treatment. Results showed that the presence of oxygenic groups improved hemocompatibility, while their removal decreased it.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2024)