期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 82, 期 2, 页码 536-542出版社
ELSEVIER
DOI: 10.1016/j.colsurfb.2010.10.013
关键词
Bacterial adhesion; Atomic force microscopy; Patterned surface; Pseudomonas biofilm; Pseudomonas fluorescens
资金
- ANPCyT [PICT 06-621, PICT 05-33225, PICT 05-32906, PAE 22771]
- UNLP [11/X532, 11/I129]
- CONICET [PIP112-200801-00362]
Biofilm development involves several stages and flagellar expression of bacteria is considered an important factor in this process. However, its role in the earliest stage of biofilm development is not yet clear. In order to analyse this topic, Pseudomonas fluorescens samples were trapped on a patterned gold surface with sub-microtrenches (ST) so as to hinder their motility, and nanostructured gold with random orientation (SR) was used as control substrate. Atomic force microscopic (AFM) observations were made on untreated samples. Initially, ca. 75% of the flagella on ST and 85% of flagella on SR are oriented towards the neighbouring bacteria. Some of them made contact and surrounded the cells. Subsequently, 2-D raft structures formed on SR inert substrates with lateral curly flagella, while those at the poles of the rafts turned towards the nearest cell group. A few flagella and the formation of 3-D bacterial structures were observed on toxic substrates like copper. Results showed that patterned substrates are suitable tools to detect the orientation of flagella in the earliest stage of biofilm formation on solid opaque surfaces avoiding sample pre-treatment. (C) 2010 Elsevier B.V. All rights reserved.
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