Electrodeposition of hyaluronic acid and hyaluronic acid-bovine serum albumin films from aqueous solutions

Hyaluronic acid (HYH) films were prepared from aqueous sodium hyaluronate (HYNa) solutions by anodic electrodeposition. The film thickness was varied in the range of 0-20 mu m by the variation of the deposition time and HYNa concentration. The deposition rate was low at HYNa concentration below 1 g L-1 and increased significantly in the range of 3-5 g L-1. The addition of bovine serum albumin (BSA) to the HYNa solutions resulted in increased deposition yield, which was attributed to the formation of composite HYH-BSA films. The thickness of the HYH-BSA films deposited by anodic electrodeposition was varied in the range of 0-80 mu m. The HYH and composite HYH-BSA films were studied by scanning electron microscopy, thermogravimetric analysis, differential thermal analysis, Fourier transform infrared spectroscopy and circular dichroism spectroscopy. The deposition mechanism and kinetics of deposition are discussed. (C) 2010 Elsevier B.V. All rights reserved.