期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 74, 期 1, 页码 370-374出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2009.07.039
关键词
Neural stem cells patterns; Poly(lactic-co-glycolic acid) film; HFBI modification; Serum; Three-dimensional device
资金
- National Natural Science Foundation of China [90403140]
Patterning of neural stem cells (NSCs) is of great importance for its potential applications in the therapy of nerve injuries. Due to the critical requirements and the great difficulty in NSCs cultivation, developing new methods for NSCs patterning is very challenging and has progressed slowly in recent years. In this study, we reported a new method for patterning NSCs on a hydrophobin II (HFBI) modified poly(lactic-co-glycolic acid) (PLGA) film by using microcontact printing (mu CP) technique. HFBI modification converted the PLGA surface from hydrophobic to hydrophilic, which should facilitate the absorption of serum on it. Serum was transferred onto the modified PLGA film by microcontact printing (mu CP) to promote NSCs adhesion on the PLGA surface. Since the serum-coated PLGA surface promoted NSCs adhesion and the serum-free PLGA surface inhibited NSCs adhesion, micro-patterns of NSCs were obtained by directly culturing NSCs on the PLGA surface patterned with serum. This method allows the precise control of NSCs adhesion on the PLGA film without using the conventional cell-repellent species, which is anticipated to make great contribution in the fields of therapy of nerve injuries. (C) 2009 Elsevier B.V. All rights reserved.
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