期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 71, 期 1, 页码 102-106出版社
ELSEVIER
DOI: 10.1016/j.colsurfb.2009.01.011
关键词
Hydrophobin; Self-assemble; Surface wettability; Choline oxidase (ChOx); Biosensor
资金
- National Natural Science Foundation of China [30772746]
- Natural Science Foundation of Tianjin [08JCZDJC20600]
- Ministry of Science and Technology of China [2006DFA32360]
Hydrophobins are small fungal proteins which self-assemble on interfaces and significantly change the surface wettability. The self-assembled film of hydrophobin HFBI on a gold surface improved the surface hydrophilicity with water contact angle changing from 73.8 +/- 1.8 degrees to 45.3 +/- 1.4 degrees. A quartz crystal microbalance (QCM) analysis indicated that the HFBI coverage density on a gold surface was 588 ng cm(-2), and the self-assembled film remained stable under different pH values ranging from 1 to 13. A hydrophilic protein such as choline oxidase (ChOx) was then successfully immobilized on the HFBI modified gold surface. To evaluate the bioactivity of immobilized enzyme, an amperometric choline biosensor was constructed based on the Gold/HFBI/ChOx electrode, which produced as large as 4578.27 nA response current by 0.238 mu g immobilized ChOx, when saturated by choline substrate. Comparing with our choline biosensors previously reported, the HFBI self-assembled film exhibited excellent capability to preserve the bioactivity of ChOx. hence a great potential in electrochemical biosensing is suggested. (C) 2009 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据