Article
Biotechnology & Applied Microbiology
Hunter N. Bomba, Abigail Carey-Ewend, Kevin T. Sheets, Alain Valdivia, Morgan Goetz, Ingrid A. Findlay, Alison Mercer-Smith, Lauren E. Kass, Simon Khagi, Shawn D. Hingtgen
Summary: Encapsulating iNSCs in the FDA-approved hemostatic matrix FLOSEAL (R) could increase their persistence, but this alone is insufficient to enhance therapeutic durability.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2022)
Article
Biotechnology & Applied Microbiology
Mathew J. Baldwin, Jolet Y. Mimpen, Adam P. Cribbs, Edward Stace, Martin Philpott, Stephanie G. Dakin, Andrew J. Carr, Sarah JB. Snelling
Summary: The topographic architecture of scaffolds can influence the cellular phenotype and transcriptome of tendon fibroblasts from both healthy and diseased tendons, which has implications for the design of scaffolds used in rotator cuff repair augmentation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Orthopedics
Phong K. K. Nguyen, Kaitlyn Hall, Iverson Holt, Catherine K. K. Kuo
Summary: This study suggests that treatment with recombinant LOX (rLOX) has minimal effects on the morphology and behaviors of developing tendon cells. This indicates that future rLOX-based therapeutic strategies can enhance tendon mechanical properties without adverse effects on tendon cell morphology and behaviors.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Yucheng Shang, Jinfeng Zeng, Michiya Matsusaki
Summary: The construction of in vitro capillary network models for drug testing and toxicity evaluation is a major challenge in tissue engineering. This study explored the effect of hydrogel stiffness on hole formation and found that smaller hole structures were formed on stiffer fibrin gels, while larger ones were formed on softer fibrin gels. By optimizing the volume of collagenase solution and incubation time, deep and small hole structures were successfully obtained.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Polymer Science
Carlos Henrique Bertoni Reis, Rogerio Leone Buchaim, Karina Torres Pomini, Abdul Latif Hamze, Isabella Vasconcelos Zattiti, Marco Antonio Hungaro Duarte, Murilo Priori Alcalde, Benedito Barraviera, Rui Seabra Ferreira Junior, Fenelon Martinho Lima Pontes, Carlos Roberto Grandini, Adriana de Cassia Ortiz, Simone Ortiz Moura Fideles, Renata Maria de Camargo Eugenio, Geraldo Marco Rosa Junior, Daniel de Bortoli Teixeira, Eliana de Souza Bastos Mazuqueli Pereira, Joao Paulo Galletti Pilon, Maria Angelica Miglino, Daniela Vieira Buchaim
Summary: This study aimed to evaluate the effect of hydroxyapatite/tricalcium phosphate (BCP) ceramic biomaterial, heterologous fibrin biopolymer (FB), and photobiomodulation (PBM) on bone defect repair. The results showed that PBM positively interfered in the repair process of bone defects filled with the biocomplex formed by FB and BCP ceramic.
Article
Polymer Science
Stavroula Kyriakou, Andreas Lubig, Cilia A. Sandhoff, Yasmin Kuhn, Stefan Jockenhoevel
Summary: In this study, a biofabrication method using multi-cell-laden cylindrical fibrin-based fibers was developed to restore the electrical signal at the atrioventricular node (AVN) for the treatment of atrioventricular block (AVB). The results showed that the fibrin hydrogels promoted the maturity of induced pluripotent stem cell cardiomyocytes (iPSC-CMs) and exhibited higher beating frequency and easier maintenance. This study highlights a feasible tissue engineering approach for AVB treatment.
Article
Pharmacology & Pharmacy
Andrew F. Uehlin, Jeremy B. Vines, Dale S. Feldman, Elijah Nyairo, Derrick R. Dean, Vinoy Thomas
Summary: In this study, a biohybrid fibrous scaffold based on nanofibers was developed and shown to have improved mechanical properties suitable for ligament-replacement grafts. The use of NaOH treatment and the immobilization of fibrin enhanced the osteogenic differentiation of cells, leading to potential applications in tissue engineering.
Article
Nanoscience & Nanotechnology
Sihao Li, Yuan Sun, Yazhou Chen, Jinwei Lu, Guangyao Jiang, Kang Yu, Yifan Wu, Yufei Mao, Hao Jin, Jikui Luo, Shurong Dong, Bin Hu, Yi Ding, An Liu, Yu Shen, Gang Feng, Shigui Yan, Yong He, Ruijian Yan
Summary: This study used an ordered 3D sandwich model to investigate the cell response in tendons and found that tendon stem/progenitor cells (TSPCs) maintained their ordering growth in the 3D model. The 3D-aligned TSPCs exhibited enhanced tenogenic differentiation and a less inflammatory phenotype compared to the 2D model, which can promote tendon regeneration.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Review
Chemistry, Multidisciplinary
Gao-Peng Dang, Wen Qin, Qian-Qian Wan, Jun-Ting Gu, Kai-Yan Wang, Zhao Mu, Bo Gao, Kai Jiao, Franklin R. Tay, Li-Na Niu
Summary: This review discusses the gradient composition of the tendon-bone interface, revealing the regulatory mechanisms and applications, with the aim of creating a mise en scene for future research in interface tissue engineering.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Engineering, Biomedical
Haiqiong Yue, Janak L. Pathak, Rui Zou, Lei Qin, Ting Liao, Yongxin Hu, Wei Kuang, Libin Zhou
Summary: In this study, a fibrin gel-based auricular scaffold containing chondrocytes/chondrocyte-microtissues was successfully fabricated for microtia reconstruction. The scaffold maintained 3D structure and auricular anatomy after in vitro culture and exhibited regenerated cartilaginous tissue after eight-week subcutaneous implantation. The overall results suggest promising potential for cartilaginous tissue regeneration, indicating an effective approach for autologous chondrocyte-based microtia reconstruction.
JOURNAL OF BIOMATERIALS APPLICATIONS
(2021)
Article
Biotechnology & Applied Microbiology
Subhajit Konar, Scott M. Bolam, Brendan Coleman, Nicola Dalbeth, Sue R. McGlashan, Sophia Leung, Jillian Cornish, Dorit Naot, David S. Musson
Summary: Tendinopathy is characterized by pathological changes in tendon matrix composition, architecture, and stiffness, with inflammation also playing an important role. This study found that substrate stiffness affects tendon-derived cells and macrophages. Tendon-derived cells showed minor responses to substrate stiffness, while macrophages exhibited a more inflammatory phenotype on non-physiological stiffness substrates. These subtle variations in matrix stiffness may contribute to the onset and progression of tendinopathy.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Food Science & Technology
Huaxin Du, Xuping Wang, Huaigu Yang, Fan Zhu, Jingrong Cheng, Xiaoxia Peng, Yaosheng Lin, Xueming Liu
Summary: The effects of mulberry pomace polysaccharide (MPP) on the physicochemical, textural, and microstructural features of yogurt during cold storage were investigated. The results showed that the appropriate addition of MPP significantly improved the texture and microstructure of yogurt, increasing gel strength and viscosity.
Article
Polymer Science
Mangang Wu, Qing Yin, Junjie Bian, Yuyu Xu, Chen Gu, Junying Jiao, Jingjing Yang, Yunlin Zhang
Summary: The study investigated the effects of Transglutaminase (TGase) on the properties of non-meat protein emulsion MP composite gels. The results showed that TGase played a crucial role in forming a tight gel network structure, enhancing water retention and overall gel strength. TGase also increased the gel formation temperature of myofibrillar proteins and facilitated cross-linking interactions between meat proteins and non-meat proteins in the composite gels. Microscopy observations demonstrated improved water-holding capacity in TGase-treated composite gels with a more uniform microstructure.
Article
Biotechnology & Applied Microbiology
Harrison L. Hiraki, Daniel L. Matera, Michael J. Rose, Robert N. Kent, Connor W. Todd, Mark E. Stout, Anya E. Wank, Maria C. Schiavone, Samuel J. DePalma, Alexander A. Zarouk, Brendon M. Baker
Summary: This study focuses on creating synthetic fiber segments through electrospinning and inducing fiber alignment in hydrogels using embedded superparamagnetic iron oxide nanoparticles. By adjusting fiber length and magnetic field strength, the degree of alignment can be controlled, leading to highly aligned fibrous hydrogel composites.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Cell Biology
Carlos J. Peniche Silva, Sebastian A. Muller, Nicholas Quirk, Patrina S. P. Poh, Carla Mayer, Antonella Motta, Claudio Migliaresi, Michael J. Coenen, Christopher H. Evans, Elizabeth R. Balmayor, Martijn van Griensven
Summary: The study investigates the use of biphasic silk fibroin scaffolds for treating injured tendon-to-bone entheses. The results suggest that the design of the transition zone plays a crucial role in promoting healing of the injured enthesis.
Article
Materials Science, Biomaterials
Constantin Edi Tanase, Omar Qutachi, Lisa J. White, Kevin M. Shakesheff, Andrew W. McCaskie, Serena M. Best, Ruth E. Cameron
REGENERATIVE BIOMATERIALS
(2019)
Article
Biology
Yassen Abbas, Lucia G. Brunel, Michael S. Hollinshead, Ridma C. Fernando, Lucy Gardner, Imogen Duncan, Ashley Moffett, Serena Best, Margherita Y. Turco, Graham J. Burton, Ruth E. Cameron
Article
Engineering, Biomedical
Giovanni S. Offeddu, Lakshana Mohee, Ruth E. Cameron
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
(2020)
Article
Multidisciplinary Sciences
Malavika Nair, Jennifer H. Shepherd, Serena M. Best, Ruth E. Cameron
JOURNAL OF THE ROYAL SOCIETY INTERFACE
(2020)
Article
Engineering, Biomedical
Malavika Nair, Ramneek K. Johal, Samir W. Hamaia, Serena M. Best, Ruth E. Cameron
Article
Materials Science, Biomaterials
Kendell M. Pawelec, Shatadru Chakravarty, Jeremy M. L. Hix, Karen L. Perry, Lodewijk van Holsbeeck, Ryan Fajardo, Erik M. Shapiro
Summary: The study examined the clinical imaging techniques for implantable medical devices, finding that adding nanoparticle contrast agents can enhance assessment effectiveness, with radiologists being able to correctly identify the location of phantoms 80% of the time.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2021)
Article
Engineering, Biomedical
Daniel Bax, Malavika Nair, Anthony S. Weiss, Richard W. Farndale, Serena M. Best, Ruth E. Cameron
Summary: This study investigates the combination of tropoelastin and collagen, showing that when combined, they alter cell behavior in a receptor-usage dependent manner. Integrin-mediated tropoelastin interactions influence cell proliferation, while non-integrin receptors affect cell spreading and proliferation. These findings provide insight into the interplay between biomaterial composition, cell surface receptors, and cell behavior, advancing tailored material design and functionality for specific cell populations.
ACTA BIOMATERIALIA
(2021)
Article
Microscopy
Matt C. Meek, Serena Best, Ruth Cameron
Summary: This paper identifies and characterizes an artifact in a commonly used filter that inaccurately increases mean pore size. Effective methods to mitigate its effects are devised, providing guidance for future studies investigating the effects of pore size.
JOURNAL OF MICROSCOPY
(2021)
Article
Physics, Applied
Malavika Nair, Ioana Bica, Serena M. Best, Ruth E. Cameron
Summary: This study investigates the impact of fabrication parameters on the microstructure of collagen-based tissue-engineering scaffolds, revealing previously unreported effects and confirming the influence of various experimental factors. The application of random forest regression for analyzing biomaterials datasets provides insights into the relative importance of input parameters on pore measurements and potential novel relationships within biomaterials. Random forest regressors demonstrate the potential to discover new relationships and design further avenues of investigation within biomaterials.
APPLIED PHYSICS REVIEWS
(2021)
Article
Engineering, Biomedical
Kyung-Ah Kwon, Daniel Bax, Jennifer H. Shepherd, Ruth E. Cameron, Serena M. Best
Summary: X-ray micro-computed tomography (mu-CT) is used to visualize the structure of hydrated collagen scaffolds by using phosphotungstic acid (PTA) as a contrast agent. The choice of processing and staining conditions is crucial for accurate visualization of crosslinked samples, while non-crosslinked or lightly crosslinked samples may cause structural artifacts.
BIOACTIVE MATERIALS
(2022)
Article
Materials Science, Multidisciplinary
David Michael Roper, Kyung-Ah Kwon, Luke Peter Malone, Serena Michelle Best, Ruth Elizabeth Cameron
Summary: This paper presents a novel method to spatially vary the birefringence of FFF parts. The effects of print speed, extrusion factor, and layer separation on birefringence are explored in single PLLA layers. The paper also demonstrates the possibility of using spatially varied birefringence to store data and presents a steganographic technique embedding information within printed parts.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Article
Engineering, Biomedical
Jamie A. Cyr, Anke Husmann, Serena M. Best, Ruth E. Cameron
Summary: The study aims to demonstrate an innovative freeze-casting system for precise architectural control of collagen scaffolds and to develop a predictive simulation as a design tool. The embedded heat sources were used to manipulate the thermal environment and achieve complex lamellar orientations in the scaffolds. The finite element model accurately predicted the freezing process and lamellar orientation. This approach offers an exciting opportunity for tailored design of regenerative scaffolds.
ACTA BIOMATERIALIA
(2022)
Article
Pharmacology & Pharmacy
Kendell M. Pawelec, Megan Varnum, Jack R. Harkema, Bruce Auerbach, Scott D. Larsen, Richard R. Neubig
Summary: This study demonstrates the potential use of MRTF/SRF inhibitors, such as CCG-257081, to prevent bleomycin-induced lung fibrosis in a clinically relevant model of the disease.
PHARMACOLOGY RESEARCH & PERSPECTIVES
(2022)
Article
Engineering, Biomedical
Kendell M. Pawelec, Ethan Tu, Shatadru Chakravarty, Jeremy M. L. Hix, Lane Buchanan, Legend Kenney, Foster Buchanan, Nandini Chatterjee, Subhashri Das, Adam Alessio, Erik M. Shapiro
Summary: Longitudinal radiological monitoring is becoming more important for biomedical devices due to the risk of device failure. Polymeric devices are not easily visible with clinical imaging, and adding nanoparticle contrast agents to polymers can solve this problem. The degradation of model nanoparticle-doped biomedical devices (phantoms) was studied over 20 weeks in simulated physiological environments. The polymer matrix and TaOx nanoparticle content significantly affect the degradation kinetics and radiopacity of the phantoms.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Nanoscience & Nanotechnology
Michael Smith, Thomas Chalklen, Cathrin Lindackers, Yonatan Calahorra, Caitlin Howe, Alkausil Tamboli, Daniel Bax, David J. Barrett, Ruth E. Cameron, Serena M. Best, Sohini Kar-Narayan
ACS APPLIED BIO MATERIALS
(2020)