Article
Chemistry, Multidisciplinary
John Z. Chen, Warren H. Finlay, Andrew Martin
Summary: This study compared in vivo and in vitro regional nasal deposition measurements and found no significant difference in deposition between the nasal drug products except for nasopharyngeal deposition with Nasonex.
PHARMACEUTICAL RESEARCH
(2022)
Article
Pharmacology & Pharmacy
Eugen Viktor Koch, Sebastian Bendas, Kristina Nehlsen, Tobias May, Stephan Reichl, Andreas Dietzel
Summary: The nasal mucosa provides a potential route for drug administration due to its accessibility and high vascularity. However, the protective functions of the mucosa, such as mucociliary clearance, pose challenges for drug candidates. In this study, a microfluidic chip based on an immortalized porcine nasal epithelial cell line was developed to mimic the physiological conditions of the nasal mucosa. The chip demonstrated effective permeation and enhanced cilia formation, making it a potential alternative to animal testing.
Article
Biotechnology & Applied Microbiology
Sergio Silva-Bea, Monica Francisco-Tome, Jorge J. Cabrera-Alvargonzalez, Carmen Potel, Maximiliano Alvarez, Sonia Perez, Benito Regueiro, Maria P. Cabral
Summary: This study characterizes Lactiplantibacillus plantarum bacteria expressing the infrared fluorescent protein IRFP713 for in vivo monitoring of their distribution in mucosal surfaces.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biophysics
Hanieh Gholizadeh, Shaokoon Cheng, Agisilaos Kourmatzis, Daniela Traini, Paul Young, Zara Sheikh, Hui Xin Ong
Summary: The current organ-on-chip platforms for respiratory drug delivery research have limitations in administering aerosol drugs and studying their interaction with the respiratory tract barrier. This study developed a human nasal epithelial mucosa (NEM)-on-a-chip that can deposit nasal aerosol formulations under realistic shear stresses. The NEM-on-a-chip demonstrated varying aerosol deposition and particle size distributions under different dynamic conditions, as well as adverse effects on cell viability at higher shear stress.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Environmental Sciences
Weiwei Wang, Xueting Wang, Jiao Xia, Xin Yang, Menglong Li, Piye Niu, Chunguang Ding, Yifei Hu, Shusheng Gong, Tian Chen
Summary: Allergic rhinitis (AR) and adenoid hypertrophy (AH) are common nasal diseases in children. Heavy metals have been identified as environmental risk factors for nasal diseases, potentially affecting the nasal mucosal microbiota. A study was conducted to explore the serum metal exposure levels and nasal mucosal microbiota composition in children with different nasal diseases, and to analyze the correlation between metal exposure and disease-related taxa. The study found that nasal diseases were associated with changes in the abundance and diversity of nasal mucosal microbiota, and metal concentrations in serum correlated with specific taxa. This research provides new evidence for the effects of metals on children's health.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Pharmacology & Pharmacy
Hanieh Gholizadeh, Hui Xin Ong, Peta Bradbury, Agisilaos Kourmatzis, Daniela Traini, Paul Young, Ming Li, Shaokoon Cheng
Summary: The NEM-on-a-chip successfully mimics the functional characteristics of human nasal mucosa, monitors drug transport in real-time, and validates its quantification method. The study highlights the importance of conducting nasal drug transport studies under physiologically relevant dynamic conditions.
EXPERT OPINION ON DRUG DELIVERY
(2021)
Article
Pharmacology & Pharmacy
Amr Seifelnasr, Xiuhua April Si, Jinxiang Xi
Summary: This study proposes a new strategy to effectively deliver high doses to the olfactory region while minimizing drug losses in other regions of the nasal cavity. Through systematic evaluation in a 3D-printed anatomical model, it was found that a head position tilting 45-60 degrees, two doses, and no inhalation flow could achieve optimal olfactory deposition. An olfactory deposition fraction of 22.7 ± 3.7% was achieved with these optimized delivery variables.
Article
Biotechnology & Applied Microbiology
Elisa Capuana, Alberto Fucarino, Stefano Burgio, Giorgia Intili, Olga Maria Manna, Alessandro Pitruzzella, Valerio Brucato, Vincenzo La Carrubba, Francesco Carfi Pavia
Summary: The development of an in vitro 3D model for the growth of nasal mucosa cells provides potential for improving therapy for individuals with chronic airway conditions. The study demonstrates that the scaffold system has a good control ability for nutrient supply and supports cell growth in the scaffold channel, ensuring cell survival and growth during the experimental time.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Pharmacology & Pharmacy
Tze-Wen Chung, Ting-Ya Wu, Zheng-Yu Siah, Der-Zen Liu
Summary: Using multifunctional nanoparticles to deliver drugs to the brain through nasal routes could overcome the blood-brain barrier and enhance delivery efficacy. Silk fibroin nanoparticles loaded with the antioxidant N-Acetyl-L-cysteine (NAC) were used as a potential delivery vehicle for NAC to the brain. The nanoparticles showed high antioxidant activity and efficient cellular uptake in in vitro and in vivo studies, leading to increased delivery of NAC to the brain.
Article
Cell & Tissue Engineering
Xiaoyi Lan, Yan Liang, Margaret Vyhlidal, Esra J. N. Erkut, Melanie Kunze, Aillette Mulet-Sierra, Martin Osswald, Khalid Ansari, Hadi Seikaly, Yaman Boluk, Adetola B. Adesida
Summary: This study investigated the impact of culture period on the formation and mechanical properties of 3D bioprinted constructs of engineered nasal cartilage using human nasal chondrocytes (hNC)-laden collagen hydrogel. The results demonstrated that these 3D bioprinted constructs were comparable or better than the clinically approved controls in terms of ECM formation and mechanical properties, making them feasible options for nasal cartilage reconstructive surgeries.
JOURNAL OF TISSUE ENGINEERING
(2022)
Article
Chemistry, Physical
Marilena Vlachou, Vangelis Karalis
Summary: The study successfully developed a new IVIVS approach to predict the outcome of bioequivalence studies, evaluated through the development process of a new generic product of amlodipine/irbesartan/hydrochlorothiazide. The approach can save time and expenses during generics development.
Article
Pharmacology & Pharmacy
Sebastian Bendas, Eugen Viktor Koch, Kristina Nehlsen, Tobias May, Andreas Dietzel, Stephan Reichl
Summary: In recent years, there has been an increase in the registration of drugs for nasal application with systemic effects. This study focused on establishing and characterizing a novel immortalized nasal epithelial cell line to improve the 3D cell culture model of the nasal mucosa. The cell line showed similar characteristics to native excised nasal mucosa, making it a promising platform for testing drug delivery systems.
Article
Multidisciplinary Sciences
Yosri Akl, Eman Kamal Ibrahim, Tareq Muhammad Algarf, Rasha R. Mostafa, Hoda M. Abdel-Hamid, Asmaa Ibrahim Muhammed
Summary: The purpose of this study was to investigate nasal histopathological changes in HP patients and identify any shared pathological features between the upper airways and HP. The study included 40 HP patients who underwent thorough evaluations and nasal mucosal biopsy. The results showed that 87.5% of HP patients had chronic inflammation of the nasal mucosa, with predominant lymphocytic infiltration in 72.5% of patients. Additionally, 77.5% of patients had a high burden of sinonasal symptoms, which was positively associated with cough severity.
SCIENTIFIC REPORTS
(2023)
Article
Instruments & Instrumentation
Ana F. F. Pereira-da-Mota, Maria Vivero-Lopez, Piyush Garg, Chau-Minh Phan, Angel Concheiro, Lyndon Jones, Carmen Alvarez-Lorenzo
Summary: This study aimed to evaluate the release profiles of two drug-loaded contact lenses (CLs) in a 3D in vitro eye blink model and compare them with in vivo profiles. The results showed that the release of both drugs was more sustained and lower in the 3D eye model compared to in vitro release in vials. The findings suggest that the current 3D printed eye blink model could be a useful tool to measure the release of ophthalmic drugs from medicated CLs, but further optimization is needed.
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2023)
Article
Cell Biology
Jelmer Legebeke, Katie L. Horton, Claire L. Jackson, Janice Coles, Amanda Harris, Htoo A. Wai, John W. Holloway, Gabrielle Wheway, Diana Baralle, Jane S. Lucas
Summary: In this study, the differentiation and recapitulation of pseudostratified epithelium in air-liquid interface (ALI) cell culture were evaluated, showing its usefulness in researching respiratory health and disease. The transcriptomic changes of ALI-culture were compared to ex vivo nasal brushing samples, highlighting the need for co-cultures with immune cell representation to better reflect the physiological state. The study also revealed the timing of ALI-culture harvest for research studies needs to be carefully considered, as different time-points correspond to different gene expressions and cell functions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Chemistry, Physical
Susanne Elisabeth Thuerer, Kai Peters, Torsten Heidenblut, Norman Heimes, Julius Peddinghaus, Florian Nuernberger, Bernd-Arno Behrens, Hans Juergen Maier, Christian Klose
Summary: In this study, semi-finished products for the manufacture of hybrid bearing bushings were produced using co-extrusion of coaxially reinforced hollow profiles. The push-out tests showed the shear strengths of hybrid hollow profiles with different material combinations. The influence of force and form closure on the joint zone was determined through shear compression tests. Thin lamellae prepared with a Focused Ion Beam were used for microstructural analysis.
Article
Materials Science, Multidisciplinary
Manuel Rodriguez Diaz, Maik Szafarska, Rene Gustus, Kai Moehwald, Hans Juergen Maier
Summary: By using a mixture of monosilane and nitrogen as the atomising and environment gas, oxide-free coatings were achieved, leading to improved adhesive tensile strength of wire arc sprayed copper coatings and a significant reduction of coating porosity.
Article
Materials Science, Multidisciplinary
Lara Vivian Fricke, Gregory Gerstein, Andreas Kotzbauer, Bernd Breidenstein, Sebastian Barton, Hans Juergen Maier
Summary: During cryogenic cutting, high strain rates result in a decrease in the content of α'-martensite and the formation of a lath-like structure. However, no α'-martensite is present in specimens subjected to compression loading. Magnetic etching proves to be an effective method in verifying the transformation of α'-martensite.
Article
Materials Science, Multidisciplinary
Lukas Munk, Silvia Reschka, Hans Juergen Maier, Peter Wriggers, Stefan Loehnert
Summary: A sharp-interface model using the extended finite element method is proposed to simulate the gamma-gamma' phase transformation in nickel-based superalloys. Combining crystal plasticity and sharp-interface theory, this model provides a good alternative to approaches based on the Cahn-Hilliard equation, and accurately captures the behavior of the interface during the phase transformation.
Article
Materials Science, Multidisciplinary
Vincent Fabian Viebranz, Thomas Hassel, Hans Juergen Maier
Summary: In this study, an iron-based FeMnAlNi shape memory alloy was processed using tungsten inert gas wire and arc additive manufacturing. The structure was cooled after each weld to maintain a constant temperature-time path, resulting in an anisotropic microstructure with promising grain orientation for high transformation strains.
Article
Materials Science, Multidisciplinary
Sebastian Barton, Maximilian K-B Weiss, Hans Juergen Maier
Summary: A new method was introduced in this study to continuously determine the microstructural changes in nickel-based materials under high-temperature fatigue. The results showed that different loading parameters can affect the maximum chromium depletion and depletion depth, which in turn affect the magnetic properties of the material.
Article
Materials Science, Multidisciplinary
Manuel Rodriguez Diaz, Selina Raumel, Marc Christopher Wurz, Maik Szafarska, Rene Gustus, Kai Moehwald, Hans Juergen Maier
Summary: Conventional thermal spraying processes in air atmosphere result in oxidation, but this study successfully applied spraying materials with high oxygen affinity in an oxide-free environment created by silane-doped inert gases, showing potential for industrial applications.
Article
Metallurgy & Metallurgical Engineering
Andreas C. Fromm, Khemais Barienti, Armin Selmanovic, Susanne E. Thuerer, Florian Nuernberger, Hans Juergen Maier, Christian Klose
Summary: Novel aluminum-copper compound castings without oxide layers were developed to enhance thermal conductivity of hybrid components. Metallurgical bonds between aluminum and copper were achieved in an atmosphere comparable to extreme high vacuum created using silane-doped inert gas. The resulting intermetallic phase seams between the joining partners contributed to significantly higher thermal conductivities compared to conventionally joined samples.
INTERNATIONAL JOURNAL OF METALCASTING
(2023)
Article
Chemistry, Physical
Jan Klett, Benedict Bongartz, Vincent Fabian Viebranz, David Kramer, Chentong Hao, Hans Juergen Maier, Thomas Hassel
Summary: This study explores the possibility of using thermal silane-doped argon plasma to reduce the oxide layer on aluminum in situ for high-quality brazing. The results show that silane-doped argon plasma can significantly reduce oxygen concentration and decrease the thickness of the artificial oxide layer.
Article
Crystallography
Seyed Vahid Sajadifar, Hans Juergen Maier, Thomas Niendorf, Guney Guven Yapici
Summary: The elevated-temperature deformation characteristics and microstructural evolution of a Ti-5V-5Mo-5Cr-4Al alloy were studied under solution-treatment conditions. The dominant softening mechanism was found to be dynamic recovery (DRV), with dynamic precipitation (DPN) occurring only at specific strain rates and temperatures. Void coalescence was observed with increasing deformation temperature and decreasing strain rate. These findings provide insights for the processing of this novel beta titanium alloy.
Article
Materials Science, Multidisciplinary
Timm Coors, Mohamad Yusuf Faqiri, Felix Saure, Christoph Kahra, Christoph Buedenbender, Julius Peddinghaus, Vannila Prasanthan, Florian Pape, Thomas Hassel, Sebastian Herbst, Florian Nuernberger, Hendrik Wester, Johanna Uhe, Bernd Breidenstein, Berend Denkena, Bernd-Arno Behrens, Gerhard Poll, Hans Juergen Maier
Summary: Additive manufacturing with multi-material design offers great possibilities for lightweight and function-integrated components. By carefully adjusting the welding process, hybrid steel-steel components with high fatigue strength can be produced. Using stainless powder material as cladding, the resulting components have a longer service life compared to traditional monolithic washers.
Article
Materials Science, Multidisciplinary
Andreas Christopher Fromm, Christoph Kahra, Armin Selmanovic, Hans Juergen Maier, Christian Klose
Summary: A metallurgical joint between aluminum and copper can be created through compound casting, providing high thermal conductivity for lightweight cooling solutions in applications such as high-power light-emitting diodes or computer processors. By employing casting in a silane-doped inert gas atmosphere with appropriate oxygen partial pressure, reoxidation of the active surfaces of aluminum and copper can be prevented, enabling direct metallurgical bonding between the two metals. This approach has achieved thermal conductivities as high as 88.3 W/m·K, and X-ray microscopy has provided insights into the relationship between microstructure and thermal properties, highlighting the impact of porosity and non-bonded areas. With the data obtained, casting parameters can be developed to ensure defect-free bonding zones and optimal heat transfer between the joined materials.
Article
Engineering, Electrical & Electronic
Markus Mlinaric, Hassen Jemaa, Thomas Hassel, Hans Juergen Maier
Summary: The present study analyzes the damage mechanisms that cause premature failure of sapphire water jet orifices using an experimental and finite element modeling approach. The research findings show that the main risk for orifices to fail prematurely is the impact of particles, rather than excessive assembly forces. The experimental data reveals that particles with a diameter of more than 10 μm are critical, and even smaller particles of 2 μm can cause damage to the orifice jewel. To prevent premature failure due to foreign particles, water filtration with a 2 μm mesh is recommended, and further research is needed to focus on the design of the interior cutting head.
Article
Materials Science, Multidisciplinary
Steffen Wackenrohr, Christof Johannes Jaime Torrent, Sebastian Herbst, Florian Nurnberger, Philipp Krooss, Christoph Ebbert, Markus Voigt, Guido Grundmeier, Thomas Niendorf, Hans Jurgen Maier
Summary: Pure iron is a biodegradable implant material with high biocompatibility, and the combination with additive manufacturing allows for flexible adjustment of microstructure to control corrosion and fatigue behavior. This study compares conventional hot-rolled pure iron with pure iron manufactured by electron beam melting, and investigates the differences in microstructure, corrosion behavior, and fatigue properties. The results show significant variations in the studied sample conditions, leading to different corrosion and fatigue responses.
NPJ MATERIALS DEGRADATION
(2022)
