Article
Chemistry, Medicinal
Eman M. E. Dokla, Nader S. Abutaleb, Sandra N. Milik, Ezzat A. E. A. Kandil, Omar M. Qassem, Yehia Elgammal, Maha Nasr, Martin J. McPhillie, Khaled A. M. Abouzid, Mohamed N. Seleem, Peter Imming, Mai Adel
Summary: Antibiotic-resistant bacteria pose a serious threat to modern medicine and human life. A study has found that compound 25d, a novel benzimidazole derivative, exhibits potent antibacterial activity against tolC-mutant Escherichia coli without significant cytotoxicity to mammalian cells. Formulation of compound 25d as lipidic nanoparticles further enhances its antibacterial activity against Gram-negative bacterial strains.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Pharmacology & Pharmacy
Nehad J. Ahmed, Mohamed A. Hassali, Ziyad S. Almalki, Abdullah U. Althemery, Mohammad Abdala, Hadeel Alahmadi, Abdul Hasseeb, Amer H. Khan
Summary: This study evaluated the antimicrobial susceptibility of bacteria in a military hospital in Alkharj, finding that Gram-negative bacteria were more prevalent. High levels of resistance were observed in Acinetobacter baumannii and several other bacteria, presenting challenges for hospital antibiotic policies. Surveillance of causative organisms and their antibiotic susceptibility remains crucial in overcoming treatment failures.
LATIN AMERICAN JOURNAL OF PHARMACY
(2021)
Article
Infectious Diseases
Qi Zhang, Shang Chen, Xiaojia Liu, Wenhan Lin, Kui Zhu
Summary: The combination of marine antibiotic equisetin and colistin shows a strong synergistic effect against Gram-negative bacteria, especially multi-drug resistant strains. Colistin promotes the intracellular accumulation of equisetin, leading to quick bacteria killing. Equisetin also restores colistin activity in an infection model, providing an alternative approach to combat Gram-negative pathogens in clinics.
Article
Infectious Diseases
Harald Seifert, Marie-Louise Von Linstow, Hester Janssen, Michael Dowzicky
Summary: The study evaluated antimicrobial resistance rates and phenotype distribution in Gram-negative isolates among pediatric patients in Europe from 2004-2012 and 2013-2018. Results showed a decrease in ESBL production in Escherichia coli and an increase in Klebsiella pneumoniae, with the highest carbapenem resistance observed in Acinetobacter baumannii. Resistance rates increased significantly in Acinetobacter baumannii and Pseudomonas aeruginosa, highlighting the importance of continued surveillance and awareness for improving treatment quality in pediatric patients.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2021)
Article
Microbiology
Thitiya Yungyuen, Tanittha Chatsuwan, Rongpong Plongla, Sakawrat Kanthawong, Umaporn Yordpratum, Supayang P. Voravuthikunchai, Sarunyou Chusri, Dennapa Saeloh, Worada Samosornsuk, Nuntra Suwantarat, Romanee Chaiwarith, Surat Wannalerdsakun, Porpon Rotjanapan, Prawat Chantharit, Orawan Tulyaprawat, Iyarit Thaipisuttikul, Pattarachai Kiratisin
Summary: The study found a serious situation of drug resistance among Gram-negative bacilli in Thailand, including important resistant strains like E. coli, K. pneumoniae, and A. baumannii. It highlights the urgent need for strict control measures to address this issue.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Review
Chemistry, Medicinal
Rajeshwari Kundar, Karuna Gokarn
Summary: This article discusses the pathogenesis of Gram-negative bacteria, the emergence of antimicrobial drug resistance, treatment failures, and the current status of the CRISPR-Cas system in diagnosing and treating Gram-negative bacterial infections.
Review
Infectious Diseases
Andrew Henderson, Evan Bursle, Adam Stewart, Patrick N. A. Harris, David Paterson, Mark D. Chatfield, Mical Paul, Yaakov Dickstein, Jesus Rodriguez-Bano, John D. Turnidge, Gunnar Kahlmeter
Summary: This study analyzed 32 randomized controlled trials and found that most trials conducted local AST, but did not report quality control, with a common practice of post-hoc referral to reference laboratories for AST. When comparing patients with infections caused by susceptible and non-susceptible pathogens in different trial groups, some antimicrobials showed better patient outcomes in cases of higher susceptibility, but there were also exceptions.
CLINICAL MICROBIOLOGY AND INFECTION
(2021)
Article
Microbiology
Farooq Ahmed Wani, Altaf Bandy, Mohammed Jayed S. Alenzi, Abdulaziz Ibrahim Alzarea, Abdullah S. Alanazi, Mohammed Ubaidullah Sayeed, Ashokkumar Thirunavukkarasu, Bilal Tantry, Mushtaq Dar
Summary: Intensive care units are complex environments that favor high resistance in microorganisms, particularly Gram-negative bacteria. This study found that a majority of infections in intensive care patients are caused by multidrug-resistant Gram-negative bacteria, with high resistance rates observed for third- and fourth-generation cephalosporins, carbapenems, and fluoroquinolones. Amikacin was identified as the most effective antibiotic, highlighting the need for enhanced surveillance and antimicrobial stewardship programs.
Review
Microbiology
Alan S. S. Cross
Summary: Infections caused by antimicrobial-resistant bacteria pose a growing threat to various medical procedures and conditions, such as surgeries, organ transplants, and cancer treatment. The development of new antimicrobials is limited, therefore, vaccines targeting antimicrobial-resistant Gram-negative bacteria may be a valuable alternative to reduce antimicrobial usage and prevent bacterial transmission.
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS
(2023)
Article
Infectious Diseases
Marta Palombo, Federica Bovo, Stefano Amadesi, Paolo Gaibani
Summary: Limited treatment options contribute to the emergence of antimicrobial resistance, and new therapeutic agents are needed for the treatment of certain pathogens. The combination of antibiotics is an effective strategy to combat multidrug-resistant infections.
Article
Microbiology
Jingyuan Xi, Peiyao Jia, Ying Zhu, Wei Yu, Jingjia Zhang, Haotian Gao, Wei Kang, Ge Zhang, Jin Li, Tong Wang, Yingchun Xu, Qiwen Yang
Summary: The study in China revealed that the main pathogens of Gram-negative bacteria bloodstream infections were Enterobacteriaceae, with Escherichia coli being the most common pathogen. Klebsiella pneumonia, Pseudomonas aeruginosa, Acinetobacter baumannii, and Enterobacter cloacae showed high resistance rates to multiple antibiotics. Polymyxin B and colistin have become crucial agents in combating infections caused by multidrug-resistant Gram-negative bacteria.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Medicine, General & Internal
Rayan I. Aloraifi, Abdullah F. Alharthi, Abdulrahman A. Almefleh, Abdulkhaleq H. Alamri, Adi S. Alobud, Reema A. Bawazeer, Abdulrahman A. Alswaji, Bassam Alalwan, Marwh G. Aldriwesh, Sameera M. Al Johani, Majed F. Alghoribi
Summary: This study investigated the occurrence and distribution of gram-negative bacteremia in five tertiary care hospitals in Saudi Arabia over a five-year period, revealing a relatively high prevalence of CRGNB. This highlights the need for strengthened antibiotic stewardship strategies and more effective infection control measures to reduce the occurrence of this issue.
CUREUS JOURNAL OF MEDICAL SCIENCE
(2023)
Article
Infectious Diseases
Nityanand Jain, Inese Jansone, Tatjana Obidenova, Raimonds Simanis, Janis Meisters, Dagnija Straupmane, Aigars Reinis
Summary: This study conducted a retrospective analysis of microbiological data from a large public hospital in Latvia from 2017 to 2020, investigating antimicrobial resistance trends for 19 gram-negative bacterial genera. It found that carbapenems were most effective against GNBs while some bacteria showed significant increases in resistance rates against certain antibiotics. Despite lower prescription levels of penicillin group antimicrobials compared to the European average, GNBs exhibited high average resistant rates, indicating the role of ESBL+ isolates in resistance rates. Constant vigilance and proper infection control measures are necessary to track emerging trends in AMR in GNBs.
Article
Infectious Diseases
James A. Karlowsky, Sibylle H. Lob, Tsz K. Khan, Wei -Ting Chen, Patrick C. Y. Woo, Wing Hong Seto, Margaret Ip, Stanley W. M. Leung, Queenie W. -L. Wongc, Rene W. Y. Chau, C. Andrew DeRyke, Katherine Young, Mary R. Motyl, Daniel F. Sahm
Summary: This study aimed to estimate carbapenem resistance in Pseudomonas aeruginosa and Enterobacterales isolated from infected patients in intensive care unit (ICU) and non-ICU hospital wards in Hong Kong. The results showed that ceftolozane/tazobactam demonstrated potent in vitro activity against both P. aeruginosa and Enterobacterales, while meropenem and imipenem had lower susceptibility rates. Carbapenemases were rare in both P. aeruginosa and Enterobacterales isolates, and most Enterobacterales had high susceptibility to meropenem and ertapenem.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2023)
Article
Immunology
Yali Gong, Yuan Peng, Xiaoqiang Luo, Cheng Zhang, Yunlong Shi, Yixin Zhang, Jun Deng, Yizhi Peng, Gaoxing Luo, Haisheng Li
Summary: This study compared the infection profile and antimicrobial resistance changes between burn intensive care unit (BICU) and burn common wards (BCW). The findings show that the types of pathogens and multidrug resistance patterns are significantly different between BICU and BCW. Over time, the gap in antimicrobial resistance between BICU and BCW has gradually narrowed.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Infectious Diseases
M. Kroemer, A. L. Clairet, K. Kabiche, A. Bendjama, X. Bertrand, S. Limat, V Nerich
Summary: The survey revealed that pharmacists believed community pharmacies had adequate resources for influenza vaccination, although some found the process complicated. Their main concerns included patient hesitancy and conflicts of interest with other healthcare professionals. Pharmacists emphasized the importance of training for pharmacy students and continuing education, as well as their willingness to increase influenza vaccination coverage for at-risk populations through vaccination.
INFECTIOUS DISEASES NOW
(2021)
Article
Infectious Diseases
Maria E. Riccio, Tess Verschuuren, Nadine Conzelmann, Daniel Martak, Alexandre Meunier, Elena Salamanca, Mercedes Delgado, Julia Guther, Silke Peter, Julian Paganini, Romain Martischang, Julien Sauser, Marlieke E. A. de Kraker, Abdessalam Cherkaoui, Ad C. Fluit, Ben S. Cooper, Didier Hocquet, Jan A. J. W. Kluytmans, Evelina Tacconelli, Jesus Rodriguez-Bano, Stephan Harbarth
Summary: The study found that ESBL-PE positive index patients discharged from hospitals are significant sources of ESBL-PE transmission within households, particularly during the first two months after discharge. The household transmission rate of ESBL-Kp was higher than ESBL-Ec, although there were more acquisitions of ESBL-Ec. Assisting the index patient with urinary and fecal excretions increased the risk of ESBL-PE transmission.
CLINICAL MICROBIOLOGY AND INFECTION
(2021)
Article
Multidisciplinary Sciences
Allison Muller, Houssein Gbaguidi-Haore, Pascal Cholley, Didier Hocquet, Marlene Sauget, Xavier Bertrand
Summary: The study confirmed the predominance of the ST131 clonal group among ESBL-producing E. coli, and identified infection and community acquisition as two independent factors associated with ST131 ESBL-producing E. coli isolates.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Pierre Edwige L. Fils, Pascal Cholley, Houssein Gbaguidi-Haore, Didier Hocquet, Marlene Sauget, Xavier Bertrand
Summary: The study identified the dissemination of pandemic clones of ESBL-Kp in a French university hospital, with genetic variability and potential cross-transmissions among patients. Substantial resistance to certain antibiotics was observed, with the prevalence of certain international epidemic clones like ST307 and ST405.
Article
Infectious Diseases
Daniel Martak, Julia Guther, Tess D. Verschuuren, Benoit Valot, Nadine Conzelmann, Stefanie Bunk, M. Eugenia Riccio, Elena Salamanca, Alexandre Meunier, Charles P. Henriot, Caroline Pressacco Brossier, Xavier Bertrand, Ben S. Cooper, Stephan Harbarth, Evelina Tacconelli, Ad C. Fluit, Jesus Rodriguez-Bano, Jan A. J. W. Kluytmans, Silke Peter, Didier Hocquet
Summary: This study in five European cities found that food items were not the main source of ESBL-Ec and ESBL-Kp, as the strains from contaminated environments were genetically distinct from those in food. The findings suggest that human-to-human transmission is possibly the most frequent route of ESBL-Ec and ESBL-Kp transmission in high-income countries.
CLINICAL MICROBIOLOGY AND INFECTION
(2022)
Article
Pharmacology & Pharmacy
A-L Clairet, J. Berthou, S. Koeberle, X. Bertrand, V Nerich, S. Limat
Summary: This study investigates the implementation of shared medication reports in pharmacy, aiming to reduce medication errors in the elderly by promoting cooperation among healthcare professionals. The results show that the new training approach was well-received by students and internship supervisors, leading to improved clinical pharmacy skills among students.
ANNALES PHARMACEUTIQUES FRANCAISES
(2022)
Article
Infectious Diseases
Daniel Martak, Houssein Gbaguidi-Haore, Alexandre Meunier, Benoit Valot, Nadine Conzelmann, Michael Eib, Ingo B. Autenrieth, Celine Slekovec, Evelina Tacconelli, Xavier Bertrand, Silke Peter, Didier Hocquet, Julia Guther
Summary: The prevalence of Pseudomonas aeruginosa intestinal carriage is high in residents of long-term care facilities, and it is mainly associated with individual-level factors. The population structure of the pathogen is predominantly polyclonal, indicating a higher frequency of individual acquisition rather than resident-to-resident transmission.
CLINICAL MICROBIOLOGY AND INFECTION
(2022)
Article
Infectious Diseases
Guillaume Miltgen, Daniel Martak, Benoit Valot, Laure Kamus, Thomas Garrigos, Guillaume Verchere, Houssein Gbaguidi-Haore, Celine Ben Cimon, Mahery Ramiandrisoa, Sandrine Picot, Anne Lignereux, Geoffrey Masson, Marie-Christine Jaffar-Bandjee, Olivier Belmonte, Eric Cardinale, Didier Hocquet, Patrick Mavingui, Xavier Bertrand
Summary: Through a study on Reunion Island, we found that despite high contamination levels in animals, they are not the main source of ESBL-Ec in humans on this densely populated, high-income island. Therefore, public health policies should primarily focus on human-to-human transmission to prevent human infections with ESBL-Ec.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Public, Environmental & Occupational Health
C. Couchoud, X. Bertrand, M. Bourgeon, G. Piton, B. Valot, D. Hocquet
Summary: This study aimed to identify the routes of acquisition of Pseudomonas aeruginosa by patients hospitalized in a medical intensive care unit (MICU) over a long period in a non-epidemic context using genome-based typing data. The findings showed that all sink traps were positive for P. aeruginosa and 7.7% of the patients acquired P. aeruginosa through patient cross-transmission.
JOURNAL OF HOSPITAL INFECTION
(2023)
Article
Engineering, Environmental
Charles P. Henriot, Helene Celle, Victor Klaba, Adrien Biguenet, Cecile Miege, Amandine Daval, Philippe Amiotte-Suchet, Jean-Charles Beugnot, Thomas Karbowiak, Xavier Bertrand
Summary: This study examined the effect of karst on antibiotic-resistant bacteria, specifically extended-spectrum beta-lactamases-producing Escherichia coli (ESBL-Ec), in a rural karst hydrosystem providing drinking water. The concentrations of ESBL-Ec were found to be spatially and temporally heterogeneous, with no filtration effect observed in the karst. The results suggest that karst poses a risk for the transmission of antibiotic-resistant bacteria.
Article
Microbiology
Kevin Bouiller, Abdeljalil Zeggay, Houssein Gbaguidi-Haore, Didier Hocquet, Catherine Chirouze, Xavier Bertrand
Summary: This study aimed to determine the prevalence and factors associated with nasal carriage of Staphylococcus aureus CC398 in the community and among hospitalized patients. The results showed a higher prevalence of MSSA CC398 in the community compared to hospitalized patients. Factors associated with nasal carriage of MSSA CC398 were primarily related to general preconditions and not related to contact with livestock.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Abdeljallil Zeggay, Alban Atchon, Benoit Valot, Didier Hocquet, Xavier Bertrand, Kevin Bouiller
Summary: Using genomic data, the study determined the origin of MRSA ST398 isolates causing invasive infections in patients with no livestock contact. The isolates were sequenced and analyzed for the presence of virulence and resistance genes. Phylogenetic analysis revealed that MSSA isolates were human-associated, while MRSA isolates were livestock-associated. The results suggest that livestock-associated MRSA acquired virulence genes, enabling them to cause invasive infections in humans.
Article
Infectious Diseases
Audrey Emery, Didier Hocquet, Richard Bonnet, Xavier Bertrand
Summary: Escherichia coli ST141 is a clonal group that is increasingly prevalent in France, although no epidemic of multidrug-resistant isolates has been reported. In a study conducted in a French University hospital, ST141 was the second most common lineage after ST131 among ESBL-producing E. coli isolates. The genetic analysis of 187 ST141 isolates revealed a low genetic diversity and two main sublineages. While many isolates had a highly virulent profile, the prevalence of antimicrobial resistance was limited. Nevertheless, surveillance of this clonal group is important due to its potential for wide community spread.
Article
Infectious Diseases
A. Biguenet, H. Bouxom, X. Bertrand, C. Slekovec
Summary: This study compared the antibiotic resistance rates of Escherichia coli, Proteus mirabilis, and Klebsiella pneumoniae in elderly patients in community, nursing home, and hospital settings. The results showed that the antibiotic resistance rate of E. coli in nursing homes was similar to that in the community setting, while the resistance rate of K. pneumoniae in nursing homes was similar to that in hospital settings. No difference in antibiotic resistance of P. mirabilis was observed between the three healthcare sectors. Therefore, patients living in nursing homes should not be considered more at risk of infection by multi-drug resistant E. coli than patients living in the community setting. Screening for multi-drug resistant K. pneumoniae may be beneficial for nursing home patients.
INFECTIOUS DISEASES NOW
(2023)
Article
Immunology
Celine Slekovec, Jerome Robert, Philippe Berthelot, Nathalie Van der Mee-Marquet, Anne-Marie Rogues, Veronique Derouin, Pascal Cholley, Xavier Bertrand, Houssein Gbaguidi-Haore
Summary: The study showed that adding contact precautions to standard precautions for Pa-positive patients with a surveillance screening program did not significantly reduce ICU-acquired Pa infections in non-outbreak situations.
CLINICAL INFECTIOUS DISEASES
(2021)