Article
Biochemistry & Molecular Biology
Maria Yoshida, Ayumu Nakashima, Naoki Ishiuchi, Kisho Miyasako, Keisuke Morimoto, Yoshiki Tanaka, Kensuke Sasaki, Satoshi Maeda, Takao Masaki
Summary: Both adipose-derived and bone marrow-derived mesenchymal stem cells (ADSCs and BMSCs) can suppress renal fibrosis, with ADSCs showing a stronger effect under serum-containing culture conditions. However, ADSC infusion in mice may lead to a higher death rate from pulmonary embolism. Therefore, the selection of ADSCs or BMSCs for clinical applications should consider the target disease and culture method.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell & Tissue Engineering
Weihui Li, Wei Wang, Xin He, Zheng Liao, Aili Aierken, Jinlian Hua, Yan Wang, Dezhang Lu, Shiqiang Zhang
Summary: The therapeutic efficacy of allogeneic adipose mesenchymal stem cell-derived extracellular vesicles (ADMSCEVs) on cat models of PR-AKI was investigated in this study. The results showed that ADMSCEVs efficiently restored the levels of creatinine, urea nitrogen, and plasma phosphorus. Blood routine analysis indicated faster recovery of leukocytes in PR-AKI cats treated with ADMSCEVs. Additionally, plasma metabolome profile analysis identified six metabolites highly correlated with the dynamic process of PR-AKI.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Cell & Tissue Engineering
Naoki Ishiuchi, Ayumu Nakashima, Satoshi Maeda, Yoshie Miura, Kisho Miyasako, Kensuke Sasaki, Toshio Uchiki, Ayano Sasaki, Shogo Nagamatsu, Naoki Nakao, Masataka Nagao, Takao Masaki
Summary: Fibrosis is a common histological feature in chronic organ injury, leading to organ failure. Adipose-derived mesenchymal stem cells (ASCs) have been studied as a therapeutic tool to prevent organ failure. This study compared the therapeutic effects of ASCs from superficial and deep layers and found that both types of ASCs are equally effective and safe for treating organ injury.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Cell Biology
Furkan Yigitbilek, Sabena M. Conley, Hui Tang, Ishran M. Saadiq, Kyra L. Jordan, Lilach O. Lerman, Timucin Taner
Summary: MSCs from liver and adipose tissue showed similar proliferation and migration properties, but had differences in immunomodulatory and pro-angiogenic abilities. Selecting cell-based therapy according to the specific characteristics of different cell sources may be important for treating certain diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jun Ho Lee, Yu Jin Won, Hail Kim, Minji Choi, Esther Lee, Bumsik Ryoou, Seok-Geun Lee, Byong Seung Cho
Summary: Exosomes derived from adipose tissue-derived stem cells (ASCs) have shown beneficial effects on cell proliferation, migration, and gene expression related to wound healing. Combining these ASC-derived exosomes (ASC-EXOs) with hyaluronic acid (HA) can enhance wound closure rates, tissue regeneration, and collagen production in animal models. This suggests the potential for their use in clinical and regenerative aesthetic applications for skin repair and regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Ahmed Abdelbaset-Ismail, Abeer Tharwat, Ahmed E. Ahmed, Tarek Khamis, Ibrahim H. Abd El-Rahim, Sadeq K. Alhag, Mohamed F. Dowidar
Summary: This study found that rat adipose-derived mesenchymal stem cells (rAD-MSCs) could alleviate diclofenac sodium (DIC)-induced acute liver injury by being transplanted into the injured liver. This treatment method significantly improved survival rate, body weight, and liver function indicators, while reducing inflammation and cell damage.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Review
Medicine, General & Internal
Robert Ossendorff, Alessandra Menon, Frank A. Schildberg, Pietro S. Randelli, Sebastian Scheidt, Christof Burger, Dieter C. Wirtz, Davide Cucchi
Summary: The global distribution and quality of studies using adipose tissue derivates in orthopedics were assessed through a systematic review. Among the 82 included studies, 70% focused on knee disorders, mainly osteoarthritis. Approximately 26% of the studies investigated expanded adipose-derived stem/stromal cells (ADSCs), with 72% of these using stromal vascular fraction (SVF). The overall quality of the studies varied, with a higher score found in studies involving expanded ADSCs compared to SVF.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Orthopedics
Xing Guo, Denghua Huang, Dan Li, Longfei Zou, Hui Lv, Yuanhui Wang, Meiyun Tan
Summary: This study found that hypoxia preconditioning attenuates the proliferation and migration ability of ADSCs, but has positive impact on tenogenic differentiation through HIF-1 alpha signaling pathway.
JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH
(2022)
Article
Agriculture, Dairy & Animal Science
Byong Seung Cho, Sung-Bae Kim, Sokho Kim, Beomseok Rhee, Jungho Yoon, Jae Won Lee
Summary: In this study, the effects of canine ASC-exosomes on canine AD were investigated using a Biostir-induced AD mouse model. The study found that cASC-EVs improved AD-like dermatitis and decreased serum IgE, ear thickness, and inflammatory cytokines such as IL-4 and IFN-γ in a dose-dependent manner. In vivo toxicity studies showed no systemic toxicity. miRNA analysis showed the presence of anti-inflammatory miRNAs in cASC-EVs, suggesting a promising cell-free therapy for treating canine AD.
Article
Cell Biology
Seyyed Ali Mard, Khojasteh Hoseinynejad, Fereshteh Nejaddehbashi
Summary: This study aimed to evaluate the influence of stem cells and gallic acid on acute renal injury caused by rhabdomyolysis. The results showed that co-treatment of stem cells and gallic acid improved kidney function, alleviated oxidative stress markers, and reduced renal tissue damage.
Review
Biochemistry & Molecular Biology
Lucy Birtwistle, Xin-Ming Chen, Carol Pollock
Summary: This review discusses mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) as a novel therapeutic approach for chronic kidney disease, highlighting their ability to promote kidney regeneration and slow down the progression of pathophysiological pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Hematology
Yukako Ono-Uruga, Yasuo Ikeda, Yumiko Matsubara
Summary: The review focuses on the application of ex vivo technologies in MK development using human adipose tissue-derived mesenchymal stem/stromal cell line (ASCL), aiming to address the clinical shortage of platelets. Manufacturing platelets from adipose tissue-derived mesenchymal stem/stromal cells (ASCs) without the need for donors may lead to sufficient platelet production within 12 days.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2021)
Article
Biochemistry & Molecular Biology
Francieli Rohden, Luciele Varaschini Teixeira, Luis Pedro Bernardi, Pamela Cristina Lukasewicz Ferreira, Mariana Colombo, Geciele Rodrigues Teixeira, Fernanda dos Santos de Oliveira, Elizabeth Obino Cirne Lima, Fatima Costa Rodrigues Guma, Diogo Onofre Souza
Summary: Nasally administered human adipose tissue mesenchymal stem cell-derived extracellular vesicles show potential neuroprotective properties in ischemic stroke, improving the blood-brain barrier, reducing infarct volume, and recovering motor and behavioral impairment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Siti Syahira Airuddin, Ahmad Sukari Halim, Wan Azman Wan Sulaiman, Ramlah Kadir, Nur Azida Mohd Nasir
Summary: Stem cells, particularly adipose-derived stem cells, have shown promising potential in disease treatment due to their differentiation abilities and secretion of growth factors. However, more research is needed to fully understand the safety and effectiveness of adipose-derived stem cells in medical treatment.
Review
Cell Biology
Yuan Liu, Christina Holmes
Summary: This review explores the tissue regeneration potential of EVs isolated from bone marrow and adipose tissue, the two most common clinical sources of adult MSCs, focusing on their angiogenic, osteogenic, and immunomodulatory potentials. Other important issues in the development of MSC-derived EV based therapies are also discussed.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Plant Sciences
Fabiana L. Silva, Soledad Quiroz-Carreno, Marcelo J. P. Ferreira, Zhao-Jun Wei, Kiran Thakur, Xingang Lv, Jose G. Avila-Acevedo, Isao Kubo, David S. Seigler, Gokhan Zengin, Kouadio Ibrahime Sinan, Adriano Mollica, Julio Alarcon-Enos, Edgar Pastene-Navarrete, Carlos L. Cespedes-Acuna
Summary: The use of plant-derived products as alternatives to synthetic products in fighting agricultural pests and microorganisms is a sustainable and safe option. This study investigated the effects of fractions from the Fabiana imbricata plant on various organisms. The results showed that stem fractions exhibited high toxicity against Drosophila melanogaster and Tenebrio molitor, while the acetate fraction showed strong antimicrobial activity. The study also found that the stem fractions had low toxicity to non-target organisms and bees.
SOUTH AFRICAN JOURNAL OF BOTANY
(2022)
Article
Engineering, Chemical
Jose Miguel Bastias-Montes, Carla Vidal-San-Martin, Yanara Tamarit-Pino, Ociel Munoz-Farina, Olga Garcia-Figueroa, Roberto Quevedo-Leon, Zhao-Jun Wei, Xingang Lv, Carlos L. Cespedes-Acuna
Summary: Cryoconcentration by centrifugation-filtration efficiently increases the content of thermosensitive bioactive compounds in maqui extract, resulting in higher antioxidant capacity.
Article
Agriculture, Multidisciplinary
Yun-Yang Zhu, Kiran Thakur, Jing-Yu Feng, Jian-Guo Zhang, Fei Hu, Carlos L. Cespedes-Acuna, Chenzhong Liao, Zhao-Jun Wei
Summary: The study reveals that B2FS can effectively alleviate the detrimental effects of ariboflavinosis, including oxidative stress-mediated liver injury, chronic intestinal inflammation, and gut dysbiosis, through the activation of the Nrf2 signaling pathway in the B-2 depletion-repletion mice model.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Food Science & Technology
Xingang Lyu, Yixuan Wang, Shiwei Gao, Xiao Wang, Wei Cao, Carlos L. Cespedes-Acuna
Summary: Sea buckthorn oil with the addition of sea buckthorn leaf extract showed improved stability and antioxidant properties. The best results were obtained when 2% SBLE was added to the SBO microcapsules prepared by spray drying. SBLE not only increased the antioxidant activity of SBO, but also showed better effectiveness compared to synthetic antioxidants.
Article
Cell Biology
Tamara Marin, Andres E. Dulcey, Fabian Campos, Catalina de la Fuente, Mariana Acuna, Juan Castro, Claudio Pinto, Maria Jose Yanez, Cristian Cortez, David W. McGrath, Pablo J. Saez, Kirill Gorshkov, Wei Zheng, Noel Southall, Maria Carmo-Fonseca, Juan Marugan, Alejandra R. Alvarez, Silvana Zanlungo
Summary: Niemann-Pick type A (NPA) disease is a fatal lysosomal neurodegenerative disorder. Recent studies have shown that c-Abl tyrosine kinase activity plays a role in neuronal damage and death in NPA disease. There is potential for the use of c-Abl inhibitors in the clinical treatment of NPA patients.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Soledad Quiroz-Carreno, Evelyn Munoz-Nunez, Fabiana L. Silva, Luis Devotto-Moreno, David S. Seigler, Edgar Pastene-Navarrete, Carlos L. Cespedes-Acuna, Julio Alarcon-Enos
Summary: Cyclopeptide alkaloids present in plants of the family Rhamnaceae, such as Discaria chacaye, were studied to establish a connection with the presence of Frankia sp. The research identified 24 alkaloids, including cyclopeptides, and found that the presence of cyclopeptide alkaloids is associated with Frankia nodules. The alkaloid extracts also showed insecticidal activity.
CHEMISTRY & BIODIVERSITY
(2022)
Article
Chemistry, Applied
Milena Terzic, Tatjana Majkic, Gokhan Zengin, Ivana Beara, Carlos L. Cespedes-Acuna, Dejan Cavic, Marija Radojkovic
Summary: The results of this study suggest that lyophilization is a better drying technique than natural convection, and microwave extraction is the most efficient technique for isolating secondary metabolites. Phenolic compounds, particularly chlorogenic acid and rutin, were found in abundance in extracts of lyophilized elderberries. Elderberry extracts showed great antioxidant and enzyme inhibition potential. These findings highlight the potential use of elderberry as a natural source of bioactive compounds for the development of dietary supplements and functional foods.
Article
Immunology
Bjoern E. Clausen, Lukas Amon, Ronald A. Backer, Luciana Berod, Tobias Bopp, Anna Brand, Sven Burgdorf, Luxia Chen, Meihong Da, Ute Distler, Regine J. Dress, Diana Dudziak, Charles-Antoine Dutertre, Christina Eich, Anna Gabele, Melanie Geiger, Florent Ginhoux, Lucila Giusiano, Gloria J. Godoy, Ahmed E. Hamouda, Lukas Hatscher, Lukas Heger, Gordon F. Heidkamp, Lola C. Hernandez, Lukas Jacobi, Tomasz Kaszubowski, Wan Ting Kong, Christian H. K. Lehmann, Tamara Lopez-Lopez, Karsten Mahnke, Dominik Nitsche, Jorg Renkawitz, Rifat A. Reza, Pablo J. Saez, Laura Schlautmann, Madeleine T. Schmitt, Anna Seichter, Malte Sielaff, Tim Sparwasser, Patrizia Stoitzner, Giorgi Tchitashvili, Stefan Tenzer, Nounagnon R. Tochoedo, Damir Vurnek, Fabian Zink, Thomas Hieronymus
Summary: This article provides a collection of protocols for the functional characterization of mouse and human dendritic cells (DC), including endocytosis and metabolism analysis, transcriptomic and proteomic characterization, migration characterization, and measurement of inflammasome and antigen (cross)-presentation activity. These protocols, written by experienced scientists and peer-reviewed by leading experts, are essential resources for basic and clinical DC immunologists.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)
Article
Plant Sciences
Natalie Vivien Gunter, Soek Sin Teh, Ibrahim Jantan, Carlos Leonardo Cespedes-Acuna, Siau Hui Mah
Summary: Cancers such as breast, colon, and lung cancers have shown alarming increases in the number of new diagnoses and mortality rates. Non-specific toxicity limits the use of current chemotherapy drugs, necessitating the development of new agents such as xanthones. Prenylated xanthones have demonstrated cytotoxic activity against cancer cells and potential as anticancer drugs, but further studies are required to establish their effectiveness and safety.
PHYTOCHEMISTRY REVIEWS
(2023)
Article
Plant Sciences
Xiao-Li Li, Xiu-Xiu Zhang, Run-Hui Ma, Zhi-Jing Ni, Kiran Thakur, Carlos L. Cespedes-Acuna, Jian-Guo Zhang, Zhao -Jun Wei
Summary: This study investigated the efficacy of PS on proliferation and migration of Endometrial carcinoma cells. Through experiments using xenograft model and Ishikawa cells, the potential molecular mechanism of dioscin was explored. The results showed that dioscin inhibited Ishikawa cell migration and invasion through the MEK/ERK and JNK signaling pathways. Hub miRNAs and genes identified in this study could be potential molecular targets for future EC treatment.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Article
Nanoscience & Nanotechnology
Dominik Kylies, Marina Zimmermann, Fabian Haas, Maria Schwerk, Malte Kuehl, Michael Brehler, Jan Czogalla, Lola C. Hernandez, Leonie Konczalla, Yusuke Okabayashi, Julia Menzel, Ilka Edenhofer, Sam Mezher, Hande Aypek, Bernhard Dumoulin, Hui Wu, Smilla Hofmann, Oliver Kretz, Nicola Wanner, Nicola M. Tomas, Susanne Krasemann, Markus Glatzel, Christoph Kuppe, Rafael Kramann, Bella Banjanin, Rebekka K. Schneider, Christopher Urbschat, Petra Arck, Nicola Gagliani, Marc van Zandvoort, Thorsten Wiech, Florian Grahammer, Pablo J. Saez, Milagros N. Wong, Stefan Bonn, Tobias B. Huber, Victor G. Puelles
Summary: Expansion microscopy physically enlarges biological specimens to achieve nanoscale resolution, but its applicability is limited due to the requirement of laser-based systems. A computational method called super-resolution radial fluctuations (SRRF) has been developed as an alternative. In this study, we introduce a workflow called expansion-enhanced super-resolution radial fluctuations (ExSRRF), which provides a resolution of up to 25 nm using LED-based widefield microscopy. ExSRRF enables molecular profiling of subcellular structures in complex clinical and experimental specimens and shows potential application in identifying pathological features.
NATURE NANOTECHNOLOGY
(2023)
Article
Plant Sciences
Ayisha Khalid, Samina Afzal, Farhan Abbas, Iftikhar Afzal, Muhammad Iqbal, Sarfraz Ahmad, Siddique Akber Ansari, Hamad M. Alkahtani, Tahir Ali Chohan, Umair Khurshid, Hammad Saleem, Carlos Leonardo Cespedes-Acuna
Summary: This study examines and compares the biological functions and chemical components of whole-plant extracts of Colutea armata made with dichloromethane and methanol solvents. The extracts show potential for inhibiting specific enzymes, containing bioactive components and antioxidants. GC-MS analysis reveals the presence of secondary metabolites in the extracts, possibly contributing to their biological activities. The extracts also exhibit antifungal and antibacterial properties. Overall, this study highlights C. armata as a valuable source of naturally bioactive compounds.
SOUTH AFRICAN JOURNAL OF BOTANY
(2023)
Article
Multidisciplinary Sciences
Marcel S. Woo, Friederike Ufer, Jana K. Sonner, Anouar Belkacemi, Joseph Tintelnot, Pablo J. Saez, Paula F. Krieg, Christina Mayer, Lars Binkle-Ladisch, Jan Broder Engler, Simone Bauer, Nina Kursawe, Vanessa Vieira, Stefanie Mannebach, Marc Freichel, Veit Flockerzi, Pablo Vargas, Manuel A. Friese
Summary: The study identifies CACNB3 as a master regulator of ATP-dependent migDC migration, which plays a crucial role in regulating tissue-specific immune responses during injury and inflammation.
Article
Biochemistry & Molecular Biology
Li Chen, Yuxin Wang, Jiaxin Liu, Zhiyuan Hong, Ka-Hing Wong, Jia-Chi Chiou, Baojun Xu, Carlos L. Cespedes-Acuna, Weibin Bai, Lingmin Tian
Summary: The study aimed to investigate the structural characteristics and in vitro fermentation patterns of polysaccharides from Boletus mushrooms. Polysaccharides were solubilized from selected mushrooms using subcritical water extraction and their physicochemical properties, monosaccharide composition, and molecular weight were analyzed. The utilization of Boletus polysaccharides by gut microbiota and their modulation of microbial communities were studied. The results showed that Boletus polysaccharides exhibited a wide range of molecular weights and were gradually utilized by the human fecal microbiota, promoting the production of SCFAs. The consumption of B. bicolor polysaccharides showed better fermentability and prebiotic effects, making it a potential approach to support gut health.
Article
Food Science & Technology
Ya-Fang Shang, Tian-Hua Zhang, Kiran Thakur, Jian-Guo Zhang, Carlos Leonardo Armando Cespedes-Acuna, Zhao-Jun Wei
Summary: This study contributes to the exploitation of L. barbarum and its meal by comparing the differences in polyphenols of L. barbarum and its meal and exploring the effects of different extraction methods.
FOOD SCIENCE AND TECHNOLOGY
(2022)