Article
Cell & Tissue Engineering
S. M. Ziauddin, Misako Nakashima, Hideto Watanabe, Michiyo Tominaga, Koichiro Iohara
Summary: This study compared the stem cell properties and pulp regenerative potential of DT-DPSCs and PT-DPSCs from the same individual. The results showed that DT-DPSCs exhibited higher expression of stem cell markers and angiogenic factors compared to PT-DPSCs. DT-DPSC-derived conditioned medium also promoted increased cell proliferation, migration, and angiogenesis. Furthermore, the regenerated pulp tissues in both DT-DPSCs and PT-DPSCs groups were similar. Therefore, DT-DPSCs could be a potential alternative to PT-DPSCs for pulp regenerative therapy.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Cell Biology
Kajohnkiart Janebodin, Rakchanok Chavanachat, Aislinn Hays, Morayma Reyes Gil
Summary: The study aimed to investigate the role of VEGFR-2 in odontoblast differentiation of dental pulp stem cells (DPSCs) by knocking down the expression of VEGFR-2. The results showed that VEGFR-2 is crucial for odontoblast differentiation in DPSCs, and deficiency of VEGFR-2 may impair the differentiation of odontoblast cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Anton L. Popov, Nadia M. Zholobak, Alexander B. Shcherbakov, Taisiya O. Kozlova, Danil D. Kolmanovich, Artem M. Ermakov, Nelli R. Popova, Nikita N. Chukavin, Ernest A. Bazikyan, Vladimir K. Ivanov
Summary: The toxic effects of cerium and fluoride species on human dental pulp stem cells and epithelial cells were studied. Cerium fluoride nanoparticles showed no cytotoxicity or genotoxicity to dental pulp stem cells and even increased cell proliferation and differentiation at certain concentrations. Furthermore, the formation of cerium fluoride provided protection against oral diseases.
Review
Biotechnology & Applied Microbiology
Hiba Asrar, Abigail S. Tucker
Summary: Major organs and tissues require close association with the vasculature during development and for later function. Recent research has revealed novel roles for endothelial cells in tissue morphogenesis and differentiation, highlighting the importance of providing a vasculature when constructing tissues and organs for tissue engineering. Important signaling pathways regulating the cross talk between endothelial cells and their environment have been identified in various organ systems.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Physical
Irina Lupse, Emoke Pall, Lucian Barbu Tudoran, Adriana Elena Bulboaca, Andreea Ciurea, Iulia Cristina Micu, Alexandra Roman, Ada Gabriela Delean, Alexandrina Muntean, Andrada Soanca
Summary: The three antimicrobial products tested showed dose-dependent cytotoxicity on gMSCs and affected cell proliferation and viability. Significant differences were observed in CCK8 values, rejecting the null hypothesis. However, there were no significant differences in MTT values between the three products.
Article
Polymer Science
Rosanna Di Tinco, Ugo Consolo, Alessandra Pisciotta, Giulia Orlandi, Giulia Bertani, Milena Nasi, Jessika Bertacchini, Gianluca Carnevale
Summary: This study evaluated the effects of three different bone substitute biomaterials (BSBs) on human dental pulp stem cells (hDPSCs). The results showed that the BSBs did not alter cell proliferation, stemness markers expression, or induce any inflammatory responses. Furthermore, the formulation of the BSBs also influenced the biological properties of the stem cells.
Review
Biochemistry & Molecular Biology
Oscar O. Solis-Castro, Marcelo N. Rivolta, Fiona M. Boissonade
Summary: Evidence suggests that dental-related stem cells (DRSCs) have the potential for ectodermal lineage differentiation and can be used in the treatment of neurodegenerative diseases and nerve injuries. However, there are limitations in current research, such as the reliance on detecting neural crest markers to classify DRSCs as NCSCs and the lack of studies proving their improved neuro-glial differentiation or applicability in relevant models. Furthermore, existing protocols need to meet manufacturing standards for clinical applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell & Tissue Engineering
Tianyi Xin, Qian Li, Rushui Bai, Ting Zhang, Yanheng Zhou, Yuehua Zhang, Bing Han, Ruili Yang
Summary: A novel frameshift mutation of SATB2 was identified in a Chinese patient with SATB2-associated syndrome (SAS) exhibiting tooth agenesis. Mechanistically, SATB2 regulates osteo/odontogenesis of human dental pulp stem cells through the Wnt/beta-catenin signaling pathway by regulating DKK1 and histone demethylase JHDM1D.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cell Biology
Rung-Shu Chen, Sheng-Hao Hsu, Hao-Hueng Chang, Min-Huey Chen
Summary: The study aimed to explore the feasibility of using adult dental pulp stem cells on polylactic acid scaffolds for tooth regeneration through experiments on dogs. The mineralization degree in the experimental group with cells seeded in the scaffolds was significantly higher than the control group, indicating the importance of dental pulp stem cells for tooth regeneration. However, there was incomplete absorption of the HA/PLA scaffolds in both groups, suggesting the need for a more rapidly absorbable material in scaffold design.
Review
Biochemistry & Molecular Biology
Christian Morsczeck
Summary: This article summarizes the molecular mechanisms driving osteogenic differentiation of dental follicle cells (DFCs) and discusses other factors that influence this process, providing valuable insights for future research and applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Bing-Chun Liu, Fang-Yuan Liu, Xin-Yue Gao, Yang-Lin Chen, Qiao-Qiao Meng, Yong-Li Song, Xi-He Li, Si-Qin Bao
Summary: The study reveals that Wnt signaling plays a crucial role in driving human pluripotent stem cells towards neural stem cells differentiation and maintaining their self-renewal. CLNSCs exhibit spinal cord properties and high proliferative capacity, providing a new cell model for investigating neurogenesis mechanisms and potential applications in regenerative medicine.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Anastasia Ariano, Francesca Posa, Giuseppina Storlino, Giorgio Mori
Summary: Teeth contain mesenchymal stem cells (MSCs) that promote tooth growth and repair. Dental-derived stem cells (d-DSCs), including dental pulp stem cells (DPSCs) and dental bud stem cells (DBSCs), have shown great potential in bone tissue engineering. The use of bone-associated factors and small molecular compounds has been found to enhance stem cell differentiation and osteogenesis. Natural and non-natural compounds found in fruits, vegetables, and drugs have also been explored for their ability to enhance MSC osteogenic differentiation and bone formation. This review focuses on the research conducted in the past decade on DPSCs and DBSCs, aiming to identify compounds that can stimulate d-DSC proliferation and osteogenic differentiation for bone regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Francesco Paduano, Elisabetta Aiello, Paul Roy Cooper, Benedetta Marrelli, Irina Makeeva, Mohammad Islam, Gianrico Spagnuolo, Davide Maged, Danila De Vito, Marco Tatullo
Summary: Dental stem cells have the potential to differentiate into various cell types and can revert back to a stem-like state after osteogenic differentiation, showing a greater potential for further differentiation. The ability to control dedifferentiation of DSCs under physiological conditions could enhance their therapeutic potential in bone tissue engineering.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Shelly Arora, Paul R. Cooper, Lara T. Friedlander, Benedict Seo, Shakila B. Rizwan, Alison M. Rich, Haizal Mohd Hussaini
Summary: This study successfully isolated and cultured cHDPCs and ncHDPCs from healthy and inflamed dental pulp tissue respectively. The growth characteristics and differentiation abilities were not significantly different between the two types of HDPCs. However, the expression of certain genes such as TLR-2 and IL-6 was significantly higher in cHDPCs, indicating an inflammatory phenotype in the inflamed dental pulp.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Physiology
Huihui Ren, Quan Wen, Qingxuan Zhao, Nan Wang, Yuming Zhao
Summary: Dental pulp plays a crucial role in maintaining tooth function and pulp-regeneration therapy shows promise in clinical practice. However, the mechanisms of pulp regeneration and the role of dental stem cells are not fully understood. This study used single-cell RNA sequencing analysis to explore the development of dental pulp and stem cells at different stages. The results revealed temporal changes in the composition of pulp cells and identified subpopulations of odontoblasts and mesenchymal stem cells. These findings may provide insights into tooth development and improve the success of dental pulp regeneration.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
J. Ver Berne, C. Politis, E. Shaheen, R. Jacobs
Summary: In this study, the cumulative effective dose and cancer risk for orthognathic surgery patients were calculated. The results showed that the cumulative effective dose was relatively low and the lifetime attributable risk of cancer was small compared to the baseline risk. These results provide a framework for interpreting radiation doses and cancer risks in orthognathic surgery patients.
INTERNATIONAL JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Sohaib Shujaat, Artur Kesztyus, Dandan Song, Tobias Regnstrand, Daniel Benchimol, Krisztian Nagy, Reinhilde Jacobs
Summary: This study provides normative skeletal measurements of the maxillary region in a Caucasian population at different ages using cone-beam computed tomography. Results show that ANS-PNS and PVD have the highest dimensional changes from 8 to 20 years of age, while AL demonstrates the least variability. Male patients have larger dimensions and show significant changes in all dimensional measurements compared to female patients.
INTERNATIONAL JOURNAL OF PAEDIATRIC DENTISTRY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Mostafa Ezeldeen, Mariano N. Simon Pedano De Piero, Lianyi Xu, Ronald B. Driesen, Jan Wyatt, Gertrude Van Gorp, Nastaran Meschi, Bart Van Meerbeek, Ivo Lambrichts, Reinhilde Jacobs
Summary: This study aimed to characterize the pattern of dental pulp healing in human teeth that underwent tooth autotransplantation (TAT) and regenerative endodontic treatment (RET) using state-of-the-art imaging techniques. The results revealed different patterns of dental pulp healing after TAT and RET.
JOURNAL OF ENDODONTICS
(2023)
Article
Multidisciplinary Sciences
Jader Camilo Pinto, Karla de Faria-Vasconcelos, Andre Ferreira Leite, Mariano Simon Pedano, Juliane Guerreiro-Tanomaru, Reinhilde Jacobs, Mario Tanomaru-Filho
Summary: The aim of this study was to evaluate the effects of foraminal enlargement on microcrack formation and apical transportation in root canals with apical curvature. Maxillary lateral incisors with apical curvature were divided into two groups, with one group undergoing foraminal enlargement. The results showed that foraminal enlargement led to the formation of new microcracks and increased apical transportation. Compared to root canal preparation up to 1 mm short of the apical foramen, foraminal enlargement using a heat-treated reciprocating file size 40 resulted in higher microcrack formation and apical transportation.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Xiaotong Wang, Sohaib Shujaat, Jan Meeus, Eman Shaheen, Paul Legrand, Pierre Lahoud, Mauricio do Nascimento Gerhardt, Reinhilde Jacobs
Summary: This in vitro study compared the accuracy and self-efficacy of novice and experienced dental surgeons using different approaches for implant placement. The findings showed that dynamic navigation (DN) significantly improved the accuracy of implant positioning. Novice practitioners scored higher with guided approaches, while experienced practitioners achieved higher scores with partial guidance and freehand techniques.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Jader Camilo Pinto, Karla de Faria Vasconcelos, Andre Ferreira Leite, Victor Aquino Wanderley, Ruben Pauwels, Matheus L. Oliveira, Reinhilde Jacobs, Mario Tanomaru-Filho
Summary: The aim of this study was to evaluate the image quality of CBCT exposure protocols and devices in detecting cracks and fine endodontic structures with metallic artifacts. Ten CBCT devices were used to scan an anthropomorphic phantom containing teeth with various structures. Different conditions were created by placing metallic objects next to the teeth of interest. The results showed that only specific CBCT devices and protocols were able to visualize cracks and fine structures effectively, while the presence of metallic artifacts significantly affected the image quality.
SCIENTIFIC REPORTS
(2023)
Article
Dentistry, Oral Surgery & Medicine
Gertrude Van Gorp, Arno Maes, Marjan Lambrechts, Reinhilde Jacobs, Dominique Declerck
Summary: This study found that paediatric dentists had poor diagnostic accuracy when using CBCT without specific training, with higher sensitivity observed when using 2D images. Sensitivity was high for diagnosing apical pathology and root fractures, but low for inflammatory root resorption, root cracks, and subluxations. Additional training in radiographic diagnosis is necessary for paediatric dentists to improve diagnostic performance, especially when using CBCT.
Article
Cell & Tissue Engineering
Florian Hermans, Lara Hemeryck, Celine Bueds, Marc Torres Pereiro, Steffie Hasevoets, Hiroto Kobayashi, Diether Lambrechts, Ivo Lambrichts, Annelies Bronckaers, Hugo Vankelecom
Summary: Organoid models have been successfully established to study tissue biology and development in a dish. In this study, tooth organoids (TOs) were developed from mouse molar and incisor, which express dental epithelium stem cell markers and recapitulate key properties of dental epithelium. These TOs can differentiate into ameloblast-resembling cells and co-differentiate into different cell types in the presence of dental mesenchymal stem cells. Furthermore, the TOs can also survive and differentiate in vivo. These organoid models provide new tools to study mouse tooth biology and development and may have implications for future human tooth repair and replacement.
Article
Dentistry, Oral Surgery & Medicine
Bahaaeldeen M. Elgarba, Stijn Van Aelst, Abdullah Swaity, Nermin Morgan, Sohaib Shujaat, Reinhilde Jacobs
Summary: This study developed a cloud-based convolutional neural network (CNN) model for automated segmentation of dental implants and attached prosthetic crowns on CBCT images. The CNN model achieved high performance and time-efficient segmentation, with high accuracy and the ability to minimize the negative impact of artifacts. This tool can enhance the creation of dental virtual models and improve presurgical planning for implants.
JOURNAL OF DENTISTRY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Jonas Ver Bernea, Soroush Baseri Saadi, Constantinus Politis, Reinhilde Jacobs
Summary: A deep learning framework was developed to differentiate between radicular cysts and periapical granulomas on panoramic imaging. The proposed model demonstrated reliable diagnostic performance, enhancing treatment and referral practices.
JOURNAL OF DENTISTRY
(2023)
Article
Nutrition & Dietetics
Sarah D'Haese, Maxim Verboven, Lize Evens, Dorien Deluyker, Ivo Lambrichts, B. O. Eijnde, Dominique Hansen, Virginie Bito
Summary: This study aimed to investigate whether and how moderate-intensity training (MIT) and high-intensity interval training (HIIT) alleviate adverse cardiac remodeling and dysfunction in type 2 diabetes (T2DM) rats. The results showed that MIT and HIIT improved insulin resistance and blood glucose levels, decreased harmful plasma advanced glycation end-products (AGEs), and reduced cardiac fibrosis, thereby improving cardiac function and optimizing mitochondrial capacity.
Article
Dentistry, Oral Surgery & Medicine
Amanda Pelegrin Candemil, Jardel Francisco Mazzi-Chaves, Matheus Lima Oliveira, Guilherme Bovi Ambrosano, Karla Farias Vasconcelos, Ruben Pauwels, Reinhilde Jacobs, Manoel Damiao Sousa-Neto
Summary: This study aimed to assess the volume of filled C-shaped root canals using different CBCT and micro-CT scanners with nano-CT as a reference. The results showed that CBCT and micro-CT scans displayed significantly larger volume of root canal filling compared to nano-CT. The presence of endodontic filling material can lead to blooming artifacts in CBCT and micro-CT images.
CLINICAL ORAL INVESTIGATIONS
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Jan Casselman, Frederic Van der Cruyssen, Frederic Vanhove, Ronald Peeters, Robert Hermans, Constantinus Politis, Reinhilde Jacobs
Summary: The study validates the reliability of 3D CRANI in visualizing extraforaminal cranial and occipital nerve branches on a 3-T system, and finds that intravenous gadolinium significantly improves MR neurography when applying this sequence.
EUROPEAN RADIOLOGY
(2023)
Review
Dentistry, Oral Surgery & Medicine
Sohaib Shujaat, Marryam Riaz, Reinhilde Jacobs
Summary: This review investigates the application of artificial intelligence in maxillofacial computer-assisted surgical planning workflows, discussing its limitations and possible future directions.
CLINICAL ORAL INVESTIGATIONS
(2023)
Article
Engineering, Biomedical
Nermin Morgan, Sohaib Shujaat, Omid Jazil, Reinhilde Jacobs
Summary: The aim of this study was to assess the symmetry of skeletal midfacial complex in skeletal class I patients. The results showed that female patients had a more symmetrical midfacial complex compared to male patients. The comparison between true and mirrored models justified the applicability of using the mirroring technique in reconstructive surgical procedures.
INTERNATIONAL JOURNAL OF COMPUTER ASSISTED RADIOLOGY AND SURGERY
(2023)
