Article
Engineering, Biomedical
Xiaogang Wang, Yuanman Yu, Luli Ji, Zhen Geng, Jing Wang, Changsheng Liu
Summary: Calcium phosphate-based materials, commonly used in bone graft substitutes, play a crucial role in bone healing. Recent studies have shown that osteoclast behaviors can greatly influence osteogenesis, with an appropriate Ca/P ratio promoting osteoclast differentiation and bone healing. This suggests the potential of manipulating osteoclast-mediated osteogenesis for improving osseointegration in materials.
BIOACTIVE MATERIALS
(2021)
Article
Biotechnology & Applied Microbiology
Ziyang Yang, Li Xie, Boqing Zhang, Gang Zhang, Fangjun Huo, Changchun Zhou, Xi Liang, Yujiang Fan, Weidong Tian, Yinghui Tan
Summary: This study demonstrates the potential of the BMP-2/PDA-BCP scaffold for the treatment of small craniofacial bone defects requiring high accuracy. The scaffold, constructed using DLP-based 3D printing, showed excellent properties and effectively promoted bone regeneration both in vitro and in vivo.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Engineering, Biomedical
Liisa T. Kuhn, Tao Peng, Gloria Gronowicz, Marja M. Hurley
Summary: Endogenous levels of fibroblast growth factor-2 (FGF-2) decrease with age, and a personalized medicine approach should guide FGF-2 supplementation to avoid excessive bone resorption and cartilage formation, which both inhibit calvarial bone repair.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2021)
Article
Materials Science, Multidisciplinary
Henri J. J. Uijlenbroek, Xingnan Lin, Xin Zhang, Liquan Deng, Daniel Wismeijer, Mingjie Wang, Lingfei Wei, Yuanna Zheng, Yuelian Liu
Summary: The study demonstrates that coating BioCaP with BMP-2 is more efficient in inducing bone formation than adsorption, with the group containing 5 μg of coating-incorporated BMP-2 showing the most bone formation, while the group with 20 μg of adsorbed BMP-2 shows the least bone formation.
Article
Materials Science, Multidisciplinary
Yifei Gu, Lingfei Wei, Zheru Zhang, Jeroen Van Dessel, Ronald B. Driesen, Ivo Lambrichts, Reinhilde Jacobs, Lei Tian, Yi Sun, Yuelian Liu, Constantinus Politis
Summary: In this study, a porous Ti6Al4V scaffold with a biomimetic coating was designed and implanted in beagle dogs. The results showed that the coating induced ectopic bone formation and increased biocompatibility of the scaffold. This combination has the potential for bone reconstruction, but further clinical studies are needed.
MATERIALS & DESIGN
(2022)
Article
Biochemistry & Molecular Biology
Ho-Kyung Lim, Ik-Jae Kwon, Sung-Woon On, Seok-Jin Hong, Byoung-Eun Yang, Soung-Min Kim, Jong-Ho Lee, Soo-Hwan Byun
Summary: The study investigated the bone regeneration capacity of BCP powder, BCP block, and BCP collagen with different concentrations of rhBMP-2 in an animal model. Results showed that increasing rhBMP-2 concentration did not significantly enhance osteogenesis, while collagen-added material may help mediate rapid bone formation in initial stages.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Materials Science, Multidisciplinary
Chia-Jen Wu, Kang-Fan Liu, Chung-Ming Liu, Wen-Chien Lan, Shu-Fen Chu, Yung-Kang Shen, Bai-Hung Huang, Jonathan Huang, Yung-Chieh Cho, Keng-Liang Ou, Pei -Wen Peng
Summary: This study successfully developed a highly pure and crystalline beta-tricalcium phosphate (b-TCP) bone graft substitute using the propagated Scleractinian coral. The coral-derived b-TCP with interconnected microporosity exhibited favorable biocompatibility and proper resorbed properties, showing promise as a bone graft substitute for bone defect repair in dental and orthopedic fields.
JOURNAL OF MATERIALS RESEARCH AND TECHNOLOGY-JMR&T
(2023)
Article
Multidisciplinary Sciences
Daisuke Tateiwa, Shinichi Nakagawa, Hiroyuki Tsukazaki, Rintaro Okada, Joe Kodama, Junichi Kushioka, Zeynep Bal, Yuichiro Ukon, Hiromasa Hirai, Takashi Kaito
Summary: NOVOSIS putty (NP), a composite material, shows great potential in promoting bone regeneration and osteoblast differentiation through the sustained release of recombinant human BMP-2. In a rat spinal fusion model, the NP group demonstrated higher volume and quality of newly formed bone compared to the collagen sponge (CS) group.
SCIENTIFIC REPORTS
(2021)
Article
Materials Science, Multidisciplinary
Yifei Gu, Yiwen Liu, Reinhilde Jacobs, Lingfei Wei, Yi Sun, Lei Tian, Yuelian Liu, Constantinus Politis
Summary: Porous scaffolds can have improved biocompatibility and osteoconductivity by using coating technique to create micro-and nanostructures on the surfaces. This study employed a biomimetic approach to coat porous titanium scaffolds with a bone morphogenetic protein-2 (BMP-2) integrated calcium phosphate (CaP) coating. In vitro characterizations demonstrated that the coated scaffolds exhibited mechanical properties similar to human cortical bone and released BMP-2 effectively. In vivo experiments on beagle dogs with mandibular defects showed that the biomimetic coating significantly promoted bone formation in the titanium scaffolds.
MATERIALS & DESIGN
(2023)
Article
Orthopedics
Nikola Stokovic, Natalia Ivanjko, Viktorija Rumenovic, Anita Breski, Kuber T. Sampath, Mihaela Peric, Marko Pecina, Slobodan Vukicevic
Summary: This study evaluated different commercially available synthetic ceramic particles as compression-resistant matrix (CRM) added to Osteogrow implants. The results showed that Osteogrow-C implants with different ceramic particles induced the formation of bone-ceramic structure and promoted bone formation. Highly porous Vitoss ceramic demonstrated the highest bone volume, while Vitoss and ChronOs ceramics increased the number of trabeculae, and BAM and Dongbo ceramics increased the thickness of trabeculae.
INTERNATIONAL ORTHOPAEDICS
(2022)
Article
Materials Science, Ceramics
Ziyu Wang, Qinghui Li, Shangxuan Ren, Hengshuo Zhang, Jie Chen, Aimin Li, Yunzhen Chen
Summary: In this study, Monetite (MC) was combined with amorphous calcium phosphate (ACP) at different ratios to form new types of bone cements. The results showed that MC75 (75% MC and 25% ACP) had better biocompatibility and osteogenic induction ability, and it met the clinical application requirements, making it a promising bone cement for repairing bone defects.
CERAMICS INTERNATIONAL
(2023)
Review
Engineering, Biomedical
Oznur Demir-Oguz, Aldo R. Boccaccini, Dagnija Loca
Summary: Calcium phosphate bone cements (CPCs) have valuable properties such as bioactivity, osteoconductivity, injectability, and moldability, but their low mechanical performance limits their clinical application. Adding bioactive glasses (BGs) as a secondary powder phase can improve the injectable properties of CPCs and enhance the degradation rate, in vitro osteogenic differentiation, cell response, and tissue-material interaction.
BIOACTIVE MATERIALS
(2023)
Review
Materials Science, Multidisciplinary
Yuanliang Xia, Hengyi Wang, Yuehong Li, Changfeng Fu
Summary: Bone defects caused by trauma, tumor, or osteomyelitis are common and can cause severe pain and limited weight-bearing ability. While bone transplantation is the gold standard, it is not suitable for all patients. Bone cement can fill any shape of bone defect and provide mechanical strength, but lacks the ability to induce bone regeneration. Various methods, such as adding bone morphogenetic proteins and mesenchymal stem cells, have been developed to enhance the bone regeneration ability of bone cement. This review aims to overview bone regeneration process, introduce improved bone cement formulations, and discuss the clinical application and future improvement of bone cement.
FRONTIERS IN MATERIALS
(2022)
Article
Chemistry, Physical
Jooseong Kim, Sukyoung Kim, Inhwan Song
Summary: This study compared the bone regeneration capacity of OCP products with two commonly used materials and found that OCP showed significantly higher resorption and new bone formation. OCP bone substitutes can enhance bone regeneration significantly by activating osteoblasts and a rapid phase transition of OCP crystals to biological apatite crystals (mineralization), as well as providing additional space for new bone formation by rapid resorption.
Article
Oncology
Yutong Liu, Dan Lin, Bo Li, Hua Hong, Chuan Jiang, Yuan Yuan, Jinwu Wang, Ruyin Hu, Bo Li, Changsheng Liu
Summary: In this study, a Dex/blood/BMP-2/CPC scaffold was fabricated for clinical bone regeneration, providing sequential release of dexamethasone and BMP-2. The scaffold effectively regulated the initial osteoimmune response and exhibited efficient osteoinductive capacity, resulting in enhanced bone regeneration in vivo. A pilot clinical trial demonstrated that the scaffold accelerated fracture healing, improved therapeutic quality, and eliminated local inflammation.
AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH
(2022)
Article
Engineering, Biomedical
Lei Wang, Hui Fu, Wenwen Wang, Yi Liu, Xumin Li, Jijing Yang, Lingli Li, Gang Wu, Yihuai Pan
Summary: Injectable colloidal gels loaded with notoginsenoside R1 were developed to induce reparative dentin formation through a simple UV method. Physicochemical examinations showed appropriate microarchitecture and mechanical properties of the Gel-MA/NGR1 hydrogel. In vivo stimulation demonstrated a significant increase in dentin formation, suggesting the potential of Gel-MA/NGR1 as a pulp-capping material.
ACTA BIOMATERIALIA
(2021)
Article
Cell Biology
Xumin Li, Haiyan Lin, Xiaorong Zhang, Richard T. Jaspers, Qihao Yu, Yinghui Ji, Tim Forouzanfar, Dongyun Wang, Shengbin Huang, Gang Wu
Summary: NGR1 can significantly attenuate oxidative stress-induced mitochondrial damage and restore osteogenic differentiation of osteoblasts by suppressing JNK signaling pathway activation, making it a promising agent in treating osteoporosis.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Wei Cao, Jianfeng Jin, Gang Wu, Nathalie Bravenboer, Marco N. Helder, Janak L. Pathak, Behrouz Zandieh-Doulabi, Jolanda M. A. Hogervorst, Shingo Matsukawa, Lester C. Geonzon, Rommel G. Bacabac, Engelbert A. J. M. Schulten, Jenneke Klein-Nulend
Summary: K-carrageenan promoted adhesion, spreading, metabolic activity, proliferation, and osteogenic differentiation of MC3T3-E1 pre-osteoblasts, indicating its potential as an osteogenic inductive factor for enhancing bone regeneration.
Article
Materials Science, Biomaterials
Shuai-shuai Cao, Shu-yi Li, Yuan-ming Geng, Kausik Kapat, Shang-bin Liu, Fidel Hugo Perera, Qian Li, Hendrik Terheyden, Gang Wu, Yue-juan Che, Pedro Miranda, Miao Zhou
Summary: Customized bone grafts using 3D printing have shown success in repairing bone defects in vivo models. Limited research has been done on clinical translations, but promising progress is expected in this area.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2021)
Article
Engineering, Multidisciplinary
Shuyi Li, Rongli Deng, Xuenong Zou, Qiong Rong, Jiali Shou, Zilong Rao, Wanqiu Wu, Gang Wu, Daping Quan, Miao Zhou, Tim Forouzanfar
Summary: The study successfully fabricated a tissue-engineered periosteum (TEP) with a continuous periosteum-specific bioactive surface and tailored physicochemical properties, promoting bone defects healing.
COMPOSITES PART B-ENGINEERING
(2022)
Article
Biochemistry & Molecular Biology
Haiyan Wang, Yongyong Yan, Haifeng Lan, Nan Wei, Zhichao Zheng, Lihong Wu, Richard T. Jaspers, Gang Wu, Janak L. Pathak
Summary: This study analyzed the effect of NGR1 on cellular activities of MSCs. The results showed that NGR1 can promote migration, adhesion, spreading, osteogenic differentiation, and anti-inflammatory properties of hASCs.
Article
Dentistry, Oral Surgery & Medicine
Dandan Ma, Wei Sun, Cuicui Fu, Kamran Nazmi, Enno C. I. Veerman, Richard T. Jaspers, Jan G. M. Bolscher, Floris J. Bikker, Gang Wu
Summary: This study revealed that histatin 1 (Hst1) in human saliva plays an important role in promoting cell activity through affecting internalization, mitochondria targeting, and activation. The research showed that various signaling pathways, including G protein-coupled receptors, endocytosis, and mitochondrial respiration, are involved in the uptake and activation of Hst1.
INTERNATIONAL JOURNAL OF ORAL SCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Menghong Li, Gang Wu, Mingjie Wang, Ernst B. Hunziker, Yuelian Liu
Summary: This study investigated the use of a biomimetic calcium phosphate coating on mini-pin implants to promote early osseointegration. The results demonstrated that the crystalline BioCaP coating served as a drug carrier with sustained release properties. Additionally, the crystalline coating significantly increased the bone to implant contact percentage at week 1. These findings suggest that the crystalline BioCaP coating may be a promising surface modification to improve the success rate of miniscrew implants in orthodontic clinics.
Article
Nanoscience & Nanotechnology
Yiwen Dong, Litao Yao, Lei Cai, Mi Jin, Tymour Forouzanfar, Lianjun Wu, Jinsong Liu, Gang Wu
Summary: In this study, an antibacterial and pro-osteogenic coaxially electrospun nanofiber guided bone regeneration (GBR) membrane was developed to meet the complex requirements of the GBR process. The membrane exhibited excellent antibacterial properties and promoted bone regeneration, making it a promising approach for patients with large-volume bone defects caused by periodontitis or peri-implantitis.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2023)
Article
Multidisciplinary Sciences
Cuicui Fu, Xiaoyun Qin, Jin Zhang, Ting Zhang, Yeqing Song, Jiaqi Yang, Gang Wu, Dan Luo, Nan Jiang, Floris J. Bikker
Summary: Spinacia oleracea-derived carbon quantum dots (CQDs) CQD-1 and CQD-2 were synthesized using different shredding methods and microwave-assisted hydrothermal approach. CQD-1 exhibited higher photoluminescent (PL) emission intensity and smaller size distribution compared to CQD-2. CQD-1 promoted osteogenic differentiation of human bone marrow-derived mesenchymal stem cells without showing toxicity or histological effects on major organs.
Article
Dentistry, Oral Surgery & Medicine
Cuicui Fu, Xiaoyun Qin, Wenlong Shao, Jin Zhang, Ting Zhang, Jiaqi Yang, Chong Ding, Yeqing Song, Xuejun Ge, Gang Wu, Floris J. J. Bikker, Nan Jiang
Summary: The therapeutic effects of carbon quantum dots (CQDs) in immunomodulation on non-obese diabetic (NOD) mice, a model for Sjogren's syndrome (SS), were evaluated. CQDs generated from Setaria viridis showed no cytotoxicity and alleviated inflammation in the submandibular glands of NOD mice. CQDs also reduced the levels of anti-SSA and anti-SSB, increased saliva flow rates, decreased CD3(+) and CD4(+) T cells, and increased the percentage of Foxp3(+) cells in NOD mice.
Article
Pharmacology & Pharmacy
Antong Wu, Janak Lal. Pathak, Xingyang Li, Wei Cao, Wenchao Zhong, Mingjing Zhu, Qiuyu Wu, Wanyi Chen, Qiao Han, Siqing Jiang, Yuzhuo Hei, Ziyi Zhang, Gang Wu, Qingbin Zhang
Summary: In this study, the efficacy of human salivary peptide histatin-1 (Hst1) in attenuating bone and cartilage damage in osteoarthritis (OA) was investigated. Hst1 was found to significantly attenuate cartilage and bone deconstruction as well as macrophage infiltration. It also triggered M1-to-M2 macrophage phenotype switching and restored the metabolic activity, migration, and chondrogenic differentiation of chondrogenic cells.
Article
Engineering, Biomedical
Yiyang Du, Menghan Chen, Jing Jiang, Lei Wang, Gang Wu, Jianying Feng
Summary: This study evaluates the effectiveness of human salivary histatin-1-functionalized methacrylic gelatin scaffold in controlling osteochondral tissue regeneration and repair in rabbits with major temporomandibular joint dimensional abnormalities.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Article
Biotechnology & Applied Microbiology
Xiaojie Tong, Jin Chen, Renqin Wang, Dan Hou, Gang Wu, Chang Liu, Janak Lal Pathak
Summary: This study investigated the direct and indirect effects of hyaluronic acid (HA) on the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) mediated by BMP-2. The study found that HA inhibited the osteogenic differentiation of BMSCs, while the secretions from HA-treated endothelial cells promoted this process.
BIOENGINEERING-BASEL
(2023)
Article
Polymer Science
Shuyi Li, Rongli Deng, Tim Forouzanfar, Gang Wu, Daping Quan, Miao Zhou
Summary: A decellularized periosteum-derived hydrogel (dPH) was fabricated and compared with Matrigel, a widely used non-bone/periosteum-derived ECM hydrogel product. It was found that dPH significantly enhanced the initial cellular spreading, proliferation, migration, and osteogenic ability of human umbilical cord mesenchymal stem cells (hUCMSCs), indicating the potential application of dPH in bone defect repair.