期刊
CLINICAL NEUROPHYSIOLOGY
卷 121, 期 8, 页码 1256-1266出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.clinph.2010.02.159
关键词
Capsaicin; Skin biopsy; Intra-epidermal nerve fibre density; Laser-evoked potentials; Quantitative sensory testing
资金
- Johnson and Johnson
Objective: To assess the temporal relationship between skin nerve denervation and regeneration (dermal and intra-epidermal fibres, IENF) and functional changes (CO2 laser-evoked potentials, LEPs, and quantitative sensory tests, QST) after topical cutaneous application of capsaicin. Methods: Capsaicin (0.075%) was applied to the lateral calf for three consecutive days. QST, LEPs and skin biopsies were performed at baseline and time intervals up to 54 days post-capsaicin treatment. Biopsies were immunostained with antibodies for PGP9.5, TRPV1, and GAP-43 (marker of regenerating nerve fibres), and analyzed for IENFs and dermal innervation (for GAP-43). Results: At 1 day post-capsaicin, cutaneous thermal sensitivity was reduced, as were LEPs. PGP9.5, TRPV1, and GAP-43 immunoreactive-nerve fibres were almost completely absent. By Day 12, LEPs had fully recovered, but PGP9.5 and TRPV1 IENF continued to be significantly decreased 54 days post-capsaicin. In contrast, dermal GAP-43 immunoreactivity closely matched recovery of LEPs. Conclusions: A good correlation was observed between LEPs and GAP-43 staining, in contrast to PGP9.5 and TRPV1. Laser stimulation is a non-invasive and sensitive method for assessing the initial IENF loss, and regenerating nerve fibres. Significance: Assessing skin biopsies by PGP9.5 immunostaining alone may miss significant diagnostic and prognostic information regarding regenerating nerve fibres, if other approaches are neglected, e. g. LEPs or GAP-43 immunostaining. (C) 2010 International Federation of Clinical Neurophysiology. Published by Elsevier Ireland Ltd. All rights reserved.
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