期刊
JOURNAL OF MASS SPECTROMETRY
卷 50, 期 4, 页码 703-710出版社
WILEY
DOI: 10.1002/jms.3579
关键词
MALDI; imaging; TOF/TOF; MS/MS; continuous raster; drug imaging; lipid imaging
资金
- National Institutes of Health/National Institute of General Medical Sciences [5P41 GM103391-03]
- Bill and Melinda Gates Foundation [N01 HD23342]
- National Institutes of Health [T32 ES007028]
- Aegis Sciences Corporation
A matrix-assisted laser desorption/ionization time of flight/time of flight tandem mass spectrometer (MALDI TOF/TOF) has been used for high-speed precursor/fragment ion transition image acquisition. High-throughput analysis is facilitated by an Nd: YLF solid state laser capable of pulse repetition rates up to 5 kHz, a high digitizer acquisition rate (up to 50pixels/s), and continuous laser raster sampling. MS/MS experiments are enabled through the use of a precision timed ion selector, second source acceleration, and a dedicated collision cell. Continuous raster sampling is shown here to facilitate rapid MS/MS ion image acquisition from thin tissue sections for the drug rifampicin and for a common kidney lipid, SM4s(d18:1/24:1). The ability to confirm the structural identity of an analyte as part of the MS/MS imaging experiment is an essential part of the analysis. Additionally, the increase in sensitivity and specificity afforded by an MS/MS approach is highly advantageous, especially when interrogating complex chemical environments such as those in biological tissues. Herein, we report continuous laser raster sampling TOF/TOF imaging methodologies which demonstrate 8 to 14-fold increases in throughput compared with existing MS/MS instrumentation, an important advantage when imaging large areas on tissues. Copyright (C) 2015 John Wiley & Sons, Ltd.
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