期刊
JOURNAL OF MASS SPECTROMETRY
卷 50, 期 12, 页码 1327-1339出版社
WILEY
DOI: 10.1002/jms.3698
关键词
electron-induced dissociation; tandem mass spectrometry; lipidomics; FT-ICR
资金
- Federal funds from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services [HHSN272201000046C]
- University of Maryland School of Pharmacy Mass Spectrometry Center [SOP1841-IQB2014]
Glycerophospholipids are a highly abundant and diverse collection of biologically relevant lipids, and distinction between isomeric and isobaric species is a fundamental aspect for confident identification. The ability to confidently assign a unique structure to a glycerophospholipid of interest is dependent on determining the number and location of the points of unsaturation and assignment of acyl chain position. The use of high-energy electrons (>20eV) to induce gas-phase dissociation of intact precursor ions results in diagnostic product ions for localizing double-bond positions and determining acyl chain assignment. We describe a high-resolution, tandem mass spectrometry method for structure characterization of glycerophospholipids using electron-induced dissociation (EID). Furthermore, the inclusion of nomenclature to systematically assign bond cleavage sites with acyl chain position and double-bond location enables a uniform platform for lipid identification. The EID methodology detailed here combines novel application of an electron-based dissociation technique with high-resolution mass spectrometry that facilitates a new experimental approach for lipid biomarker discovery and validation. Copyright (c) 2015 John Wiley & Sons, Ltd.
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