期刊
CLINICAL MICROBIOLOGY AND INFECTION
卷 20, 期 12, 页码 O1106-O1112出版社
WILEY-BLACKWELL
DOI: 10.1111/1469-0691.12715
关键词
Carbapenem resistance; Gram-negative bacilli; MALDI-TOF MS; microarray; MS-MS spectrometry; PCR
资金
- Swiss National Science Foundation (REQUIP) [326000-121314/1]
- UNIL-CHUV
- Laboratory of Clinical Pharmacology, Service of Biomedicine CHUV, Lausanne
Carbapenemases should be accurately and rapidly detected, given their possible epidemiological spread and their impact on treatment options. Here, we developed a simple, easy and rapid matrix-assisted laser desorption ionization-time of flight (MALDI-TOF)-based assay to detect carbapenemases and compared this innovative test with four other diagnostic approaches on 47 clinical isolates. Tandem mass spectrometry (MS-MS) was also used to determine accurately the amount of antibiotic present in the supernatant after 1h of incubation and both MALDI-TOF and MS-MS approaches exhibited a 100% sensitivity and a 100% specificity. By comparison, molecular genetic techniques (Check-MDR Carba PCR and Check-MDR CT103 microarray) showed a 90.5% sensitivity and a 100% specificity, as two strains of Aeromonas were not detected because their chromosomal carbapenemase is not targeted by probes used in both kits. Altogether, this innovative MALDI-TOF-based approach that uses a stable 10-g disk of ertapenem was highly efficient in detecting carbapenemase, with a sensitivity higher than that of PCR and microarray.
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