Article
Microbiology
Francis Simo-Fouda, Laetitia Ninove, Lea Luciani, Christine Zandotti, Celine Gazin, Remi N. Charrel, Antoine Nougairede
Summary: The study retrospectively evaluated the rotavirus and adenovirus ICT routinely used in the virology laboratory at the University Hospital of Marseille, France. The results showed that the ICT had high sensitivity and specificity for rotavirus detection, but low sensitivity for adenovirus detection, indicating that a PCR-based assay is better suited for adenovirus detection in stools.
Article
Virology
Manon Broutin, Fleur Costa, Sandy Peltier, Jennifer Maye, Nicolas Versille, Bernard Klonjkowski
Summary: There is a need for highly effective vaccines for veterinary infectious diseases. Canine adenovirus type 2 (CAV2) vectors have shown potential in developing multiple vaccines. In this study, a CAV2 vector encoding the rabies virus glycoprotein was tested with and without an oil-based adjuvant. The adjuvant increased rabies antibody titers and did not affect the anti-CAV2 serological responses. The study also identified host antiviral genes modulated after vaccination, revealing the up-regulation of certain signaling pathways.
Article
Virology
Ellen Kathrin Link, Matthias Eddicks, Liangliang Nan, Mathias Ritzmann, Gerd Sutter, Robert Fux
Summary: Genotyping of PCV2 is important for diagnosis and epidemiological studies. The new qPCR system introduced in this study is ideal for mass testing and can be a valuable complement to PCV2 sequencing, especially in cases of simultaneous infections with multiple PCV2 genotypes.
Article
Ecology
Marco Di Domenico, Valentina Curini, Riccardo Caprioli, Carla Giansante, Agata Mrugala, Michaela Mojzisova, Cesare Camma, Adam Petrusek
Summary: The study developed real-time PCR TaqMan assays to distinguish five genotype groups of A. astaci. This method may be a useful tool for investigating the natural history of crayfish plague in Europe, as well as the sources and introduction pathways of the pathogen.
FRONTIERS IN ECOLOGY AND EVOLUTION
(2021)
Article
Oncology
Qiumei Yao, Yinlei Bai, Shaji Kumar, Elaine Au, Alberto Orfao, Chor Sang Chim
Summary: In this study, clonotype identification in multiple myeloma patients using ASO RQ-PCR and NGS was compared. Results showed high similarity in clonotypes identified by both methods, with NGS demonstrating higher sensitivity. However, the applicability of NGS could be improved by enhancing the primers used.
FRONTIERS IN ONCOLOGY
(2021)
Article
Medicine, General & Internal
Isabel Legaz, Maria Victoria Bernardo, Rafael Alfaro, Helios Martinez-Banaclocha, Jose Antonio Galian, Victor Jimenez-Coll, Francisco Boix, Anna Mrowiec, Diego Salmeron, Carmen Botella, Antonio Parrado, Maria Rosa Moya-Quiles, Alfredo Minguela, Santiago Llorente, Jesus de la Pena-Moral, Manuel Muro
Summary: The study found a higher number of over-expressed mTOR pathway genes than under-expressed ones in biopsies from rejected kidney transplants. The molecular function of both types of transcripts (over/under expressed) is different in the rejected patients, indicating that further studies are needed to determine if variations in gene expression profiles can act as predictors of graft loss.
FRONTIERS IN MEDICINE
(2021)
Article
Veterinary Sciences
Xinxiu Qian, Liping Hu, Kaichuang Shi, Haina Wei, Yuwen Shi, Xin Hu, Qingan Zhou, Shuping Feng, Feng Long, Shenglan Mo, Zongqiang Li
Summary: This study developed a triplex real-time quantitative PCR (qPCR) method for detection and differentiation of genotypes I and II ASFV. The method demonstrated high sensitivity, specificity, and good repeatability. Testing of clinical samples from Guangxi province revealed a high prevalence of ASFV in the region, with a higher detection rate for genotype II compared to genotype I.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Genetics & Heredity
Yufeng Wang, Xihui Chen, Qi Chen, Tangdong Chen, Kun Chen, Yuanming Wu, Li Wang
Summary: This study described a sensitive TaqMan real-time PCR method for screening the HNA-3 genotype and using ddPCR to identify blood donors with the rare HNA-3B antigen and calculate SLC44A2 allele frequency in mixed populations. The new method was highly sensitive and specific, successfully detecting the rare HNA-3B antigen and inferring the SLC44A2 frequency.
FRONTIERS IN GENETICS
(2022)
Article
Microbiology
Gulcan Gurses, Nebiye Yentur Doni, Fadile Yildiz Zeyrek, Akin Yigin
Summary: In this study, the Sybr Green-based ITS-1 real time polymerase chain reaction (Rt-PCR) method was used to genotype 136 samples from suspected Leishmania patients in Sanliurfa, Turkey. The results showed that the ITS-1 Rt-PCR method had the highest positivity rate and could genotype the Leishmania specimens as L.tropica and L.major. This method may be useful for the detection of Leishmania parasites and rapid identification of Leishmania species.
MIKROBIYOLOJI BULTENI
(2022)
Article
Biochemical Research Methods
Rikke Lind Jorgensen, Martin Schou Pedersen, Alisha Shazad Chauhan, Louise Munkholm Andreasson, Gitte Qvist Kristiansen, Jan Gorm Lisby, Maiken Worsoe Rosenstierne, Kristian Schonning
Summary: By adding detergent to the rRT-PCR master mix, it is possible to detect SARS-CoV-2 directly in eSwab samples without the need for NA purification. The study results showed that direct lysis rRT-PCR detected SARS-CoV-2 in 27 out of 30 previously identified positive samples, demonstrating high accuracy.
JOURNAL OF VIROLOGICAL METHODS
(2021)
Article
Medical Laboratory Technology
Kuenyoul Park, Soo Jin Yoo
Summary: By retrospectively reviewing PCR test records from a hospital and analyzing genetic data from a healthy Korean population, it was found that real-time PCR for CYP2C19 genotyping remains clinically useful but may miss patients with decreased gene function.
LABORATORY MEDICINE
(2023)
Article
Biology
Serafeim C. Chaintoutis, Taxiarchis Chassalevris, Sofia Balaska, Evangelia Mouchtaropoulou, George Tsiolas, Ioannis Vlatakis, Areti Tychala, Dimitris Koutsioulis, Anagnostis Argiriou, Lemonia Skoura, Chrysostomos Dovas
Summary: The study describes a one-step real-time RT-PCR assay using LNA modified TaqMan probes for rapid identification of key mutations in SARS-CoV-2 variants, aiding in public health interventions.
Article
Food Science & Technology
Wenjun Wang, Tiean Wei, Manna Shi, Yu Han, Yang Shen, Xiang Zhou, Bang Liu
Summary: A novel UP-M-rtPCR approach was developed for the specific identification and quantification of cat, dog, fox, and mink fractions in meat samples. It showed good accuracy and precision for quantitative analysis and improved efficiency in identifying multiple animal-derived ingredients in meat.
Article
Genetics & Heredity
O. Yu. Limanskaya, O. P. Limanskii
Summary: The hairpin probe has a higher fluorescence quenching efficiency than the linear probe in real-time PCR, resulting in a lower background fluorescence and a higher signal to noise ratio. Experimental comparison showed that the interaction between the quencher and fluorophore differs for probes in different conformations. The linear probe uses inductive-resonance energy transfer (FRET) for quenching, while the hairpin probe uses contact quenching with a closer arrangement of the fluorophore and quencher, but a resonant energy transfer is also possible. The absorption spectra of the probes and their complexes indicate a dynamic mechanism of energy transfer for the linear probe and a contact mechanism for the hairpin probe. The energy migration radius R was calculated as 32.4 angstrom for the hairpin probe and 47.3 angstrom for the linear probe.
CYTOLOGY AND GENETICS
(2023)
Article
Biotechnology & Applied Microbiology
Yusuke Goto, Kazuhiro Fukunari, Shigekatsu Tada, Satoki Ichimura, Yuzumi Chiba, Tohru Suzuki
Summary: The study developed a multiplex real-time RT-PCR system that can simultaneously detect nine viruses and seven bacteria associated with swine respiratory disease. The system showed comparable detection sensitivity to single RT-qPCR for all target pathogens, and the primers and probes used in the system were highly specific. The system also demonstrated its usefulness in detecting the causative pathogens in samples collected from healthy pigs and pigs with respiratory disorders in the field.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)
Article
Immunology
Laura Toivonen, Juho Vuononvirta, Jussi Mertsola, Matti Waris, Qiushui He, Ville Peltola
PEDIATRIC INFECTIOUS DISEASE JOURNAL
(2017)
Article
Chemistry, Multidisciplinary
Satu Lahtinen, Stefan Krause, Riikka Arppe, Tero Soukka, Tom Vosch
CHEMISTRY-A EUROPEAN JOURNAL
(2018)
Article
Chemistry, Multidisciplinary
Riikka Arppe-Tabbara, Miguel R. Carro-Temboury, Casper Hempel, Tom Vosch, Thomas Just Sorensen
CHEMISTRY-A EUROPEAN JOURNAL
(2018)
Article
Chemistry, Multidisciplinary
Emilia Palo, Satu Lahtinen, Henna Pakkila, Mikko Salomaki, Tero Soukka, Mika Lastusaari
Article
Multidisciplinary Sciences
Miguel R. Carro-Temboury, Riikka Arppe, Tom Vosch, Thomas Just Sorensen
Article
Chemistry, Analytical
Stefan Krause, Mads Koerstz, Riikka Arppe-Tabbara, Tero Soukka, Tom Vosch
METHODS AND APPLICATIONS IN FLUORESCENCE
(2018)
Article
Chemistry, Physical
Emilia Palo, Mikko Salomaki, Mika Lastusaari
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2019)
Article
Microbiology
Anna Kazakova, Laura Kakkola, Henna Pakkila, Tamara Teros-Jaakkola, Tero Soukka, Ville Peltola, Matti Waris, Ilkka Julkunen
Letter
Rheumatology
Olli Ruuskanen, Matti Waris
ARTHRITIS & RHEUMATOLOGY
(2022)
Article
Microbiology
Miia Laine, Jaakko Ahti, Ville Peltola, Piritta Peri, Antti J. Hakanen, Matti Waris
Summary: The study compared the diagnostic efficacy and tolerability of two types of nasopharyngeal swabs for COVID-19 testing. Results showed that the molded plastic swab was comparable to the standard flocked swab in terms of efficacy for respiratory virus detection and patient tolerance during sampling.
MICROBIOLOGY SPECTRUM
(2021)
Article
Sport Sciences
Olli Ruuskanen, Raakel Luoto, Maarit Valtonen, Olli J. Heinonen, Matti Waris
Summary: Upper respiratory tract infections are common among elite athletes, but the impact of exercise on susceptibility to infections and the clinical implications of immune system changes need further virological studies.
Article
Microbiology
Pekka Kolehmainen, Jemna Heroum, Pinja Jalkanen, Moona Huttunen, Laura Toivonen, Varpu Marjomaki, Matti Waris, Teemu Smura, Laura Kakkola, Sisko Tauriainen, Ville Peltola, Ilkka Julkunen
Summary: Seasonal coronaviruses cause respiratory infections in children, and the duration of antibody levels following the first infections is limited. This study found that seasonal coronavirus infections and reinfections are common in early childhood, and the antibody levels decline relatively rapidly.
MICROBIOLOGY SPECTRUM
(2022)
Article
Virology
Niklas Lindblad, Timo Hanninen, Maarit Valtonen, Olli J. Heinonen, Matti Waris, Olli Ruuskanen
Summary: Influenza A outbreaks occurred in two professional hockey teams after they played against each other, resulting in 13 players and 2 staff members falling ill during the prevalent COVID-19 period. Seven players missed an important game due to influenza. Rapid diagnosis enabled effective control of the outbreaks through pharmaceutical and nonpharmaceutical measures.
Article
Sport Sciences
Raakel Luoto, Olli Ruuskanen, Johanna K. Ihalainen, Satu Pekkala, Jukka Hintikka, Noora Kanerva, Matti Waris, Olli J. Heinonen, Maarit Valtonen
Summary: The study found that Finnish elite cross-country skiers had lower concentrations of CRP but higher concentrations of IL-10, TNF-alpha, and IFN-gamma compared to moderately-exercising controls. There was no significant difference in IL-6 and GlycA levels between athletes and controls.
JOURNAL OF SCIENCE IN SPORT AND EXERCISE
(2023)
Article
Nanoscience & Nanotechnology
Emilia Palo, Hongbo Zhang, Mika Lastusaari, Mikko Salomaki
ACS APPLIED NANO MATERIALS
(2020)