期刊
CLINICAL MICROBIOLOGY AND INFECTION
卷 18, 期 -, 页码 8-11出版社
WILEY-BLACKWELL
DOI: 10.1111/j.1469-0691.2012.03862.x
关键词
Horizontal gene transfer; mobile genetic elements; plasmid isolation; pyrosequencing; wastewater metagenome
资金
- Lundbeck Foundation [R44-2009-4384] Funding Source: researchfish
Clin Microbiol Infect 2012; 18 (Suppl. 4): 811 Abstract A metamobilome is defined as a metagenome of circular genetic elements within a certain community. Metagenomic analyses of plasmids provide insights into the composition and structure of environmental plasmid communities. It is a promising method that will provide information about the types of plasmids that are present within environmental samples, and will give overviews about occurrences of plasmids as well as accessory genetic elements carried on these plasmids. A metamobilome library was constructed by combining multiple displacement amplification with pyrosequencing. This method provided a fast, efficient and unbiased strategy to investigate the communal gene pool of circular genetic elements (the metamobilome). We compared our wastewater metamobilome library with a wastewater metagenome library, against chromosomes, plasmids, phages and IS element databases, respectively. This showed that very few strictly chromosomal reads were present in our metamobilome library. Furthermore, data analysis showed that our library was strongly enriched for genes encoding plasmid-selfish traits, such as stability and conjugation, and most strikingly several hundred new putative plasmid replicases have been recovered.
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