Article
Microbiology
Simona Presente, Christine Bonnal, Anne-Cecile Normand, Yannick Gaudonnet, Arnaud Fekkar, Jean-Francois Timsit, Solen Kerneis
Summary: We report the first identification of a fluconazole-resistant Candida parapsilosis (FR-Cp) strain in our hospital, which subsequently caused an outbreak involving 17 patients (12 deaths) within a 26-bed French intensive care unit. Microsatellite genotyping confirmed that all FR-Cp isolates belonged to the same clone.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Medicine, General & Internal
Gaole Yuan, Yingqiu Tu, Lili Liu, Tiantian Xu
Summary: This study aimed to investigate the efficacy of fluconazole combined with caspofungin in treating Candida bloodstream infection in premature infants. Results showed that the treatment regimen successfully eliminated persistent candidemia without significant adverse effects. While the results were promising, further large-scale clinical trials are needed to validate the safety and efficacy of this treatment approach.
Article
Microbiology
Parisa Badiee, Teun Boekhout, Pardis Haddadi, Rasoul Mohammadi, Abdolkarim Ghadimi-Moghadam, Jafar Soltani, Ali Zarei Mahmoudabadi, Seyyed Amin Ayatollahi Mousavi, Mohammad Javad Najafzadeh, Kambiz Diba, Ali Reza Salimi-Khorashad, Maneli Amin Shahidi, Fatemeh Ghasemi, Hadis Jafarian
Summary: This study evaluated the antifungal activities of eight antifungal agents against Candida species isolated from 10 university hospitals in Iran. The most frequently isolated species were Candida albicans and Candida glabrata. Caspofungin and voriconazole were found to be the most effective antifungal agents for treating Candida infections in the region.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Naemah Alkhars, Anthony Gaca, Yan Zeng, Nisreen Al-Jallad, Elena Rustchenko, Tong Tong Wu, Eli Eliav, Jin Xiao
Summary: This study aimed to evaluate the susceptibility of oral Candida isolates to antifungal agents, compare susceptibility between isolates from mothers and children, assess longitudinal changes in susceptibility, and detect mutations in antifungal resistance genes in Candida albicans. The results showed that caspofungin, fluconazole, and nystatin were effective against Candida, while two missense mutations in the CDR2 gene were associated with nystatin resistance. Most children's isolates had similar susceptibility to those from their mothers, and caspofungin resistance increased over time. The study suggests the need for novel antifungal regimens to control oral yeast in infants.
Review
Infectious Diseases
Vasiliki Soulountsi, Theodoros Schizodimos, Serafeim Chrysovalantis Kotoulas
Summary: In recent decades, invasive candidiasis (IC) has become a significant cause of morbidity, mortality, and economic burden in the intensive care unit (ICU). The epidemiology of IC remains a difficult and unsolved puzzle for the literature. IC burden estimation is challenging due to various definitions and limitations in case-finding methodologies. Candidemia and intra-abdominal candidiasis (IAC) are the predominant types of IC in the ICU, with an increasing incidence of candidemia, particularly in elderly or immunosuppressed patients, and a shift towards non-albicans species worldwide.
Article
Chemistry, Analytical
Chen Chen, Yi Wang, Fan Wu, Weili Hong
Summary: This study demonstrates rapid antifungal susceptibility testing (AFST) by measuring the metabolism in single fungal cells. The results show that metabolism change can serve as a biomarker for rapid AFST, with a 100% categorical agreement with the gold standard broth microdilution test. Additionally, a protocol is developed for direct AFST from positive blood cultures, overcoming the limitation of slow growth in conventional methods and providing potential for rapid diagnosis of fungal infections.
ANALYTICAL CHEMISTRY
(2023)
Article
Microbiology
Joao N. de Almeida, Elaine C. Francisco, Ferry Hagen, Igor B. Brandao, Felicidade M. Pereira, Pedro H. Presta Dias, Magda M. de Miranda Costa, Regiane T. de Souza Jordao, Theun de Groot, Arnaldo L. Colombo
Summary: In December 2020, Candida auris emerged in Brazil, with the first two patients colonized in the same COVID-19 intensive care unit. Antifungal susceptibility testing showed low minimal inhibitory concentrations for various drugs. Microsatellite typing revealed clonal strains belonging to the South Asian clade of C. auris. The hypothesis is that the species was introduced several months before the first recognized case and/or emerged locally in the coastline Salvador area during the COVID-19 pandemic.
Article
Biochemistry & Molecular Biology
Stefan Mijatovic, Jelena Antic Stankovic, Ivana Colovic Calovski, Eleonora Dubljanin, Dejan Pljevljakusic, Dubravka Bigovic, Aleksandar Dzamic
Summary: The aim of this study was to determine the susceptibility of Candida albicans sputum isolates on fluconazole and caspofungin, as well as the antifungal potential of Lavandula angustifolia essential oil (LAEO). The results showed that LAEO effectively inhibited the growth of C. albicans and showed equal effectiveness against fluconazole and caspofungin susceptible strains.
Review
Infectious Diseases
Sveva Di Franco, Aniello Alfieri, Marco Fiore, Ciro Fittipaldi, Vincenzo Pota, Francesco Coppolino, Pasquale Sansone, Maria Caterina Pace, Maria Beatrice Passavanti
Summary: This comprehensive review focuses on the healthcare problem of secondary peritonitis caused by multidrug-resistant pathogens, specifically carbapenem-resistant Enterobacterales (CRE). The paper highlights the importance of antimicrobial stewardship and precision medicine in combating antimicrobial resistance. It also emphasizes the need for cautious use of new drugs effective against CRE in empirical therapy for secondary peritonitis.
Article
Microbiology
Natalie S. Nunnally, Tajah Damm, Shawn R. Lockhart, Elizabeth L. Berkow
Summary: The CLSI M44ed3E disk diffusion method showed overall categorical agreement with the CLSI M27ed4 broth microdilution method in evaluating the susceptibility of Candida auris isolates. The concordant results for caspofungin and fluconazole were high, while there were varying levels of errors for amphotericin B. The disk diffusion method provides an accurate way to determine susceptibility for caspofungin and fluconazole, as well as identifying a majority of amphotericin B-susceptible isolates.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Review
Infectious Diseases
Omar Simonetti, Verena Zerbato, Sara Sincovich, Lavinia Cosimi, Francesca Zorat, Venera Costantino, Manuela Di Santolo, Marina Busetti, Stefano Di Bella, Luigi Principe, Roberto Luzzati
Summary: We report a case of C. lipolytica bloodstream infection in a 53-year-old man with alcohol withdrawal syndrome and mild COVID-19. He had been treated with broad-spectrum antimicrobials. Echocardiography ruled out infective endocarditis and PET/CT was negative for other deep-seated fungal infections. The patient was discharged after blood culture clearance and clinical healing.
Article
Medicine, General & Internal
Yuri Song, Myoung Soo Kim, Jin Chung, Hee Sam Na
Summary: This study used next-generation sequencing to simultaneously analyze bacterial and fungal communities and found that oropharyngeal microbes play an important etiologic role in ventilator-associated pneumonia and Candida infections.
Article
Microbiology
Malgorzata Mikulska, Laura Magnasco, Alessio Signori, Chiara Sepulcri, Silvia Dettori, Stefania Tutino, Antonio Vena, Franca Miletich, Nadir Ullah, Paola Morici, Lorenzo Ball, Paolo Pelosi, Anna Marchese, Daniele Roberto Giacobbe, Matteo Bassetti
Summary: The sensitivity of serum BDG for diagnosing candidemia in ICU patients varies depending on the causative Candida species. Infections caused by C. parapsilosis and C. auris may result in lower BDG levels.
Article
Dermatology
Carl Hinrichs, Miriam Wiese-Posselt, Barbara Graf, Christine Geffers, Beate Weikert, Philipp Enghard, Alexander Aldejohann, Annette Schrauder, Andreas Knaust, Kai-Uwe Eckardt, Petra Gastmeier, Oliver Kurzai
Summary: This article describes the first documented patient-to-patient transmission of Candida auris in a COVID-19 intensive care unit (ICU) in Germany and the infection control measures implemented to prevent further spread of the pathogen.
Article
Infectious Diseases
Ruth Van Daele, Roger J. Bruggemann, Erwin Dreesen, Pieter Depuydt, Bart Rijnders, Frederic Cotton, David Fage, Matthias Gijsen, Kenny Van Zwam, Yves Debaveye, Joost Wauters, Isabel Spriet
Summary: In critically ill patients undergoing ECMO treatment with posaconazole, pharmacokinetic parameters were consistent and reached the targeted PTA for prophylaxis but not for treatment of invasive aspergillosis. Drug monitoring is recommended to adjust dosages accordingly.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Juergen Prattes, Joost Wauters, Daniele Roberto Giacobbe, Jon Salmanton-Garcia, Johan Maertens, Marc Bourgeois, Marijke Reynders, Lynn Rutsaert, Niels Van Regenmortel, Piet Lormans, Simon Feys, Alexander Christian Reisinger, Oliver A. Cornely, Tobias Lahmer, Maricela Valerio, Laurence Delhaes, Kauser Jabeen, Joerg Steinmann, Mathilde Chamula, Matteo Bassetti, Stefan Hatzl, Riina Rautemaa-Richardson, Philipp Koehler, Katrien Lagrou, Martin Hoenigl
Summary: Coronavirus disease 2019 (COVID-19)-associated pulmonary aspergillosis (CAPA) is a common complication among critically ill COVID-19 patients in ICU. It is more prevalent in older patients, those receiving invasive ventilation, and those receiving tocilizumab treatment. CAPA is an independent predictor of ICU mortality.
CLINICAL MICROBIOLOGY AND INFECTION
(2022)
Article
Infectious Diseases
Gert Van der Auwera, Leigh Davidsson, Pierre Buffet, Marie-Therese Ruf, Marina Gramiccia, Stefania Varani, Carmen Chicharro, Aldert Bart, Gundel Harms, Peter L. Chiodini, Hanne Brekke, Florence Robert-Gangneux, Sofia Cortes, Jaco J. Verweij, Alessandra Scarabello, Sara Karlsson Sobirk, Romain Guery, Saskia van Henten, Trentina Di Muccio, Elena Carra, Pieter van Thiel, Martin Vandeputte, Valeria Gaspari, Johannes Blum
Summary: This study collected retrospectively records of leishmaniasis cases diagnosed in 15 centers between 2014 and 2019, and analyzed data on country of infection, reason for traveling, infecting species, age, and sex. The results showed that visceral leishmaniasis was mainly acquired in Europe, while cutaneous leishmaniasis was primarily imported from outside Europe. The geographic species distribution largely confirmed known epidemiology, with some exceptions. The study highlights the importance of pooling species typing data from multiple centers to monitor changing epidemiology.
Article
Microbiology
Brice Autier, Juergen Prattes, P. Lewis White, Maricela Valerio, Marina Machado, Jessica Price, Matthias Egger, Jean-Pierre Gangneux, Martin Hoenigl
Summary: This multicenter study evaluated the performance of the IMMY Aspergillus Galactomannan Lateral Flow Assay (LFA) for the diagnosis of pulmonary aspergillosis in patients with COVID-19-related acute respiratory failure, showing good sensitivity when used from respiratory samples but limited sensitivity in serum, possibly due to weak invasiveness during CAPA. Further mycological investigations are recommended for isolated results slightly above the cutoff.
JOURNAL OF CLINICAL MICROBIOLOGY
(2022)
Article
Infectious Diseases
A. Serris, J. Benzakoun, F. Danion, R. Porcher, R. Sonneville, M. Wolff, S. Kremer, V Letscher-Bru, A. Fekkar, G. Hekimian, V Pourcher, M-E Bougnoux, S. Poiree, F. Ader, F. Persat, Francois Cotton, Pierre Tattevin, J-P Gangneux, L. Lelievre, S. Cassaing, Fabrice Bonneville, S. Houze, Stephane Bretagne, R. Herbrecht, O. Lortholary, O. Naggara, F. Lanternier
Summary: This study retrospectively analyzed 119 cases of cerebral aspergillosis and identified two distinct disease patterns, hematogenous dissemination and contiguous dissemination, based on the route of spread. Hematogenous dissemination was associated with impaired consciousness, multiple abscesses, and microangiopathy, while contiguous dissemination was associated with cranial nerve palsy and evidence of meningitis. Hematogenous dissemination, hematological malignancy, and detection of serum galactomannan were associated with higher six-week mortality.
JOURNAL OF INFECTION
(2022)
Article
Critical Care Medicine
Jean-Pierre Gangneux, Eric Dannaoui, Arnaud Fekkar, Charles-Edouard Luyt, Francoise Botterel, Nicolas De Prost, Jean-Marc Tadie, Florian Reizine, Sandrine Houze, Jean-Francois Timsit, Xavier Iriart, Beatrice Riu-Poulenc, Boualem Sendid, Saad Nseir, Florence Persat, Florent Wallet, Patrice Le Pape, Emmanuel Canet, Ana Novara, Melek Manai, Estelle Cateau, Arnaud W. Thille, Sophie Brun, Yves Cohen, Alexandre Alanio, Bruno Megarbane, Muriel Cornet, Nicolas Terzi, Lionel Lamhaut, Estelle Sabourin, Guillaume Desoubeaux, Stephan Ehrmann, Christophe Hennequin, Guillaume Voiriot, Gilles Nevez, Cecile Aubron, Valerie Letscher-Bru, Ferhat Meziani, Marion Blaize, Julien Mayaux, Antoine Monsel, Frederique Boquel, Florence Robert-Gangneux, Yves Le Tulzo, Philippe Seguin, Helene Guegan, Brice Autier, Matthieu Lesouhaitier, Romain Pelletier, Sorya Belaz, Christine Bonnal, Antoine Berry, Jordan Leroy, Nadine Francois, Jean-Christophe Richard, Sylvie Paulus, Laurent Argaud, Damien Dupont, Jean Menotti, Florent Morio, Marie Soulie, Carole Schwebel, Cecile Garnaud, Juliette Guitard, Solene Le Gal, Dorothee Quinio, Jeff Morcet, Bruno Laviolle, Jean-Ralph Zahar, Marie-Elisabeth Bougnoux
Summary: This study demonstrates a high prevalence of invasive pulmonary aspergillosis and candidemia in mechanically ventilated patients with severe COVID-19, and a high mortality associated with pr/pb CAPA. These findings highlight the importance of active surveillance for fungal pathogens in patients with severe COVID-19.
LANCET RESPIRATORY MEDICINE
(2022)
Letter
Critical Care Medicine
Jean-Pierre Gangneux, Jeff Morcet, Bruno Laviolle, Eric Dannaoui, Jean-Francois Timsit, Stephane Ruckly, Jean-Ralph Zahar, Marie-Elisabeth Bougnoux
LANCET RESPIRATORY MEDICINE
(2022)
Review
Infectious Diseases
Candida Driemeyer, Diego R. Falci, Rita O. Oladele, Felix Bongomin, Bright K. Ocansey, Nelesh P. Govender, Martin Hoenigl, Jean Pierre Gangneux, Cornelia Lass-Florl, Oliver A. Cornely, Alexandre Alanio, Jesus Guinea, C. Orla Morrissey, Riina Rautemaa-Richardson, Arunaloke Chakrabarti, Jacques F. Meis, Caroline Bruns, Jannik Stemler, Alessandro C. Pasqualotto
Summary: Africa is a favorable environment for fungal infections due to high risk factors, but there are significant challenges in terms of laboratory infrastructure and availability of antifungal drugs in many African countries.
Article
Microbiology
Laurene Tardieu, Gillian Divard, Olivier Lortholary, Anne Scemla, Eric Rondeau, Isabelle Accoceberry, Remi Agbonon, Alexandre Alanio, Adela Angoulvant, Laetitia Albano, Philippe Attias, Anne Pauline Bellanger, Dominique Bertrand, Julie Bonhomme, Francoise Botterel, Nicolas Bouvier, Matthias Buchler, Taieb Chouaki, Thomas Crepin, Marie-Fleur Durieux, Guillaume Desoubeaux, Gary Doppelt, Loic Favennec, Arnaud Fekkar, Ophelie Fourdinier, Marie Frimat, Jean-Pierre Gangneux, Claire Garandeau, Lilia Hasseine, Christophe Hennequin, Xavier Iriart, Nassim Kamar, Hannah Kaminski, Raphael Kormann, Laurence Lachaud, Christophe Legendre, Moglie Le Quintrec Donnette, Jordan Leroy, Charlene Levi, Marie Machouart, David Marx, Jean Menotti, Valerie Moal, Florent Morio, Natacha Mrozek, Muriel Nicolas, Philippe Poirier, Marie-Noelle Peraldi, Benjamin Poussot, Stephane Ranque, Jean-Philippe Rerolle, Boualem Sendid, Renaud Snanoudj, Jerome Tourret, Marc Vasse, Cecile Vigneau, Odile Villard, Laurent Mesnard, Fanny Lanternier, Cedric Rafat
Summary: This study analyzed the clinical characteristics of cryptococcosis in kidney transplant recipients in France. The incidence of cryptococcal meningitis (CM) was relatively high, and the prognosis was poor. Hyponatremia and neurological symptoms were associated with CM.
Editorial Material
Dermatology
A. Maruani, S. Barbarot, J. P. Gangneux, M. Caseris, C. Moreau, S. Brun, F. Botterel, J. Menotti, J. Toubiana, L. Chouchana, M. Beylot-Barry, N. Dupin, B. Guillot, O. Chosidow
ANNALES DE DERMATOLOGIE ET DE VENEREOLOGIE
(2022)
Review
Radiology, Nuclear Medicine & Medical Imaging
Wonju Hong, P. Lewis White, Matthijs Backx, Jean-Pierre Gangneux, Florian Reizine, Philipp Koehler, Robbert G. Bentvelsen, Maria Lujan Cuestas, Hamed Fakhim, Jung Im Jung, Young Kyung Lee, Nishil R. Dalsania, Ravi Karan Patti, Soon Ho Yoon
Summary: This study conducted a systematic review and individual patient-level image analysis of CT findings of COVID-19-associated pulmonary aspergillosis (CAPA), revealing that CAPA can frequently manifest as COVID-19 pneumonia without common CT abnormalities of pulmonary aspergillosis. If abnormalities exist on CT images, CAPA may frequently accompany bronchial abnormalities.
Article
Infectious Diseases
Hedvig Glans, Leif Dotevall, Gert Van der Auwera, Aldert Bart, Johannes Blum, Pierre Buffet, Romain Guery, Jean-Pierre Gangneux, Saskia van Henten, Gundel Harms, Stefania Varani, Florence Robert-Gangneux, Robert Rongisch, Bjorn Andersson, Maria Bradley
Summary: This article describes and evaluates the treatment outcomes of cutaneous leishmaniasis (CL) among travelers and migrants in Europe caused by Leishmania major and Leishmania tropica. The study found that L. major infections were mostly found in individuals visiting friends and relatives, while L. tropica infections were mainly identified in migrants. Pentavalent antimony had a cure rate of 73% (L. major) and 78% (L. tropica) among patients.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Infectious Diseases
Gregoire Pasquier, Magalie Demar, Patrick Lami, Asma Zribi, Pierre Marty, Pierre Buffet, Nicole Desbois-Nogard, Jean Pierre Gangneux, Stephane Simon, Romain Blaizot, Pierre Couppie, Louis Thiebaut, Francine Pratlong, Jean-Pierre Dedet, Patrick Bastien, Yvon Sterkers, Christophe Ravel, Laurence Lachaud
Summary: Leishmaniasis shows different epidemiology in metropolitan France and its overseas territories. Autochthonous cases have decreased while imported cases have increased over time. French Guiana has a high incidence, while sporadic cases are reported in the French West Indies. Continued monitoring and active surveillance are necessary.
PLOS NEGLECTED TROPICAL DISEASES
(2022)
Article
Infectious Diseases
Victor Mercier, Valerie Letscher-Bru, Marie-Elisabeth Bougnoux, Laurence Delhaes, Francoise Botterel, Daniele Maubon, Frederic Dalle, Alexandre Alanio, Sandrine Houze, Eric Dannaoui, Carole Cassagne, Sophie Cassaing, Marie-Fleur Durieux, Arnaud Fekkar, Jean-Philippe Bouchara, Jean-Pierre Gangneux, Julie Bonhomme, Damien Dupont, Damien Costa, Boualem Sendid, Taieb Chouaki, Nathalie Bourgeois, Antoine Huguenin, Sophie Brun, Caroline Mahinc, Lilia Hasseine, Solene Le Gal, Anne-Pauline Bellanger, Eric Bailly, Florent Morio, Celine Nourrisson, Nicole Desbois-Nogard, Estelle Perraud-Cateau, Anne Debourgogne, Helene Yera, Laurence Lachaud, Milene Sasso
Summary: The study aimed to determine the epidemiological cut-off values (ECVs) of ten antifungal agents in a wide range of yeasts and Aspergillus spp. using gradient concentration strips. Retrospective collection of minimum inhibitory concentrations for various antifungal agents was done from 35 French microbiology laboratories between 2002 and 2020. The calculated ECVs can aid in interpreting minimum inhibitory concentrations and identifying non-wild type isolates.
CLINICAL MICROBIOLOGY AND INFECTION
(2023)
Article
Environmental Sciences
M. Cogliati, S. Arikan-Akdagli, A. Barac, A. C. Bostanaru, S. Brito, N. Cerikcioglu, M. A. Efstratiou, C. Ergin, M. C. Esposto, M. Frenkel, J. P. Gungneux, A. Gitto, C. I. Goncalves, H. Guegan, N. Gunde-Cimerman, M. Guran, E. Jonikaite, M. Katarzyte, L. Klingspor, M. Mares, W. G. Meijer, W. J. G. Melchers, J. Meletiadis, V. Nastasa, M. Novak Babic, D. Ogunc, B. Ozhak, A. Prigitano, S. Ranque, L. Romano, R. O. Rusu, R. Sabino, A. Sampaio, S. Silva, J. H. Stephens, M. Tehupeiory-Kooreman, A. Velegraki, C. Verissimo, E. Segal, J. Brandao
Summary: The study used data from the Mycosands survey to investigate the factors affecting the distribution of yeasts and molds along European shores. Environmental factors such as climate, soil properties, and water conditions were analyzed using species distribution modelling. The results showed that yeasts and molds have different distributions along European shores, with yeasts being more tolerant of low temperatures and molds showing affinity with specific soil compositions and water conditions.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Review
Infectious Diseases
Jon Salmanton-Garcia, Martin Hoenigl, Jean-Pierre Gangneux, Esther Segal, Ana Alastruey-Izquierdo, Sevtap Arikan Akdagli, Katrien Lagrou, Volkan oezenci, Antonio Vena, Oliver A. Cornely
Summary: The diagnostic capacity for invasive fungal infections in Europe is generally good, but there are limitations in access to certain diagnostic tools and antifungal drugs. These limitations need to be addressed to ensure optimal diagnosis and treatment for all patients.
