4.7 Article

Direct pathogen detection from swab samples using a new high-throughput sequencing technology

期刊

CLINICAL MICROBIOLOGY AND INFECTION
卷 17, 期 2, 页码 241-244

出版社

WILEY-BLACKWELL
DOI: 10.1111/j.1469-0691.2010.03246.x

关键词

Emerging infectious diseases; high-throughput sequencing technology; novel influenza A (H1N1) virus; Solexa system; viral detection

资金

  1. 'AIDS and viral hepatitis and other major infectious diseases prevention and control' technology projects [2009ZX10004-102]
  2. National Basic Research Program of China [2010CB534003, 2005CB522905]
  3. Institute of Pathogen Biology [2008IPB010]

向作者/读者索取更多资源

P>The detection of emerging infectious diseases has been a continuing concern, especially with the novel influenza A (H1N1) viral pandemic of 2009. In the present study, we validated a 'second-generation' parallel sequencing platform for viral detection in swab samples collected during recent influenza virus infections in Beijing. This operation yielded millions of valid reads per sample and resulted in an almost complete spectrum of nucleotide information. Importantly, novel A (H1N1) and seasonal A (H3N2) influenza virus-derived sequences were detected without prior knowledge or use of genetic information in advance, suggesting that this approach could be a valuable tool for diagnosing emerging infectious diseases.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据