Article
Immunology
Ying Yu, Bo Hu, Huanhuan Fan, Hailing Zhang, Shizhen Lian, Hongye Li, Shuangshuang Li, Xijun Yan, Shaohui Wang, Xue Bai
Summary: The study identified molecular epidemiology and biological characteristics of Escherichia coli associated with hemorrhagic pneumonia (HP) in mink from five Chinese provinces. Analysis included serotyping, antimicrobial susceptibility, detection of virulence genes, phylogenetic grouping, whole-genome sequencing, drug resistant gene, multilocus sequence typing (MLST), and biofilm formation assays.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Infectious Diseases
Rosalino Vazquez-Lopez, Tanya Hernandez-Martinez, Selene Ivonne Larios-Fernandez, Celia Pina-Leyva, Manuel Lara-Lozano, Tayde Guerrero-Gonzalez, Javier Martinez-Bautista, Eduardo Gomez-Conde, Juan Antonio Gonzalez-Barrios
Summary: This study investigated the conformation of the beta-lactam resistome in Escherichia coli strains causing nosocomial infections. The results showed a multi-drug-resistance phenotype against beta-lactam drugs, primarily driven by the expression of the blaTEM gene family.
Article
Infectious Diseases
Antonella Santona, Jose Joao Sumbana, Maura Fiamma, Massimo Deligios, Elisa Taviani, Samuel Elija Simbine, Tomas Zimba, Jahit Sacarlal, Salvatore Rubino, Bianca Paglietti
Summary: ESBL-producing ExPEC, including high-risk lineages, were identified in extraintestinal infections at Maputo Central Hospital. The majority of isolates showed multidrug resistance, with ESBL genes associated with resistance to cefotaxime and ceftazidime. Furthermore, different sequence types were found to carry CTX-M transposition units, potentially facilitating the dissemination of ESBL genes. Genomic analysis revealed a diverse array of virulence-associated genes and clonality among isolates, suggesting the presence of hospital-acquired infections and long-term persistence.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2022)
Article
Immunology
Liyun Liu, Ling Zhang, Haijian Zhou, Min Yuan, Dalong Hu, Yonglu Wang, Hui Sun, Jianguo Xu, Ruiting Lan
Summary: This prospective study investigated molecular characteristics and antimicrobial susceptibility patterns of Citrobacter spp. isolates from extraintestinal infections. The results showed a diverse range of Citrobacter isolates causing extraintestinal infections and a high prevalence of multidrug resistance.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Food Science & Technology
Priyanka Priyanka, Prem Raj Meena, Dharma Raj, Anuj Rana, Akshay Dhanokar, K. Siddaardha Duggirala, Arvind Pratap Singh
Summary: This study systematically examined various plant-origin foods for the presence of Extraintestinal pathogenic Escherichia coli (ExPEC) and specific putative ExPEC pathotypes. The findings revealed the presence of ExPEC isolates and specific putative ExPEC pathotypes such as Uropa-thogenic E. coli and Septicemia-associated E. coli. The study also highlighted the presence of multidrug resistance in these ExPEC pathotypes, posing a potential threat to human health and food safety.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Microbiology
Antoine Bridier-Nahmias, Adrien Launay, Alexandre Bleibtreu, Melanie Magnan, Violaine Walewski, Jeremie Chatel, Sara Dion, Veronique Robbe-Saute, Olivier Clermont, Francoise Norel, Erick Denamur, Olivier Tenaillon
Summary: Little is known about the dynamics of adaptation in acute bacterial infections. By sequencing multiple isolates from monoclonal extraintestinal Escherichia coli infections in several patients, we were able to uncover traces of selection taking place at short time scales compared to chronic infection. High genomic diversity was observed in the patient isolates, with an excess of nonsynonymous mutations, and the comparison within and between different infections showed patterns of convergence at the gene level, both constituting strong signs of adaptation. The genes targeted were coding mostly for proteins involved in global regulation, metabolism, and adhesion/motility. Moreover, virulence assessed in a mouse model of sepsis was variable among the isolates of single patients, but this difference was left unexplained at the molecular level. This work gives us clues about the E. coli lifestyle transition between commensalism and pathogenicity.
Editorial Material
Microbiology
Johann D. D. Pitout
Summary: Escherichia coli is a major cause of bloodstream infections worldwide. A longitudinal study in a Madrid hospital found that certain E. coli B2 phylogroups dominated the community E. coli population and were often introduced into hospital settings. Longitudinal surveys from England and Canada also showed that certain ST131 subclades were responsible for increased resistance to fluoroquinolones and cephalosporins in E. coli during the mid- to late 2000s.
Article
Infectious Diseases
Isabella F. S. Figueiredo, Lorena G. Araujo, Raissa G. Assuncao, Itaynara L. Dutra, Johnny R. Nascimento, Fabricia S. Rego, Carolina S. Rolim, Leylane S. R. Alves, Mariana A. Frazao, Samilly F. Cadete, Luis Claudio N. da Silva, Joicy C. de Sa, Eduardo M. de Sousa, Waldir P. Elias, Flavia R. F. Nascimento, Afonso G. Abreu
Summary: The study showed that cinnamaldehyde has potential in treating sepsis by reducing inflammation, increasing cell production in bone marrow and spleen, and improving survival rates in animals.
Article
Infectious Diseases
Dan Li, Pei Li, Xiaoyan Yu, Xuefei Zhang, Qinglan Guo, Xiaogang Xu, Minggui Wang, Minghua Wang
Summary: This study investigated the molecular characteristics of Escherichia coli causing bloodstream infections in Huashan Hospital, Shanghai, China from 2010 to 2015. The results showed that E. coli strains isolated from BSI were still highly susceptible to carbapenems and beta-lactamase inhibitor combinations, with bla(CTX-M) being the dominant genotype of ESBLs. The low expression of bla(KPC) may be the reason for the lower resistance of E. coli to carbapenems.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Infectious Diseases
D. J. Astley, N. Masters, A. Kuballa, M. Katouli
Summary: This study found that AIEC strains are common in the gut of healthy individuals and community-acquired infection patients, posing a potential risk to individuals predisposed to IBD, with these strains also detected in sewage treatment plants and surface waters.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2021)
Article
Microbiology
Joshua L. Cherry
Summary: Enterohemorrhagic E. coli (EHEC) is a major cause of human illness, death, and economic loss. Understanding the genetic changes in EHEC and its source of contamination is crucial for effective mitigation. Through whole-genome sequencing, it was found that EHEC experienced genetic changes mainly in adhesion-related sequences, potentially leading to altered adhesive properties and increased virulence. This pattern of genetic change may help identify other bacteria threatening human health. Additionally, the presence of a mutation affecting arsenic resistance regulation suggests an arsenic-contaminated groundwater as the persistent source of contamination. This observation can aid in locating the reservoir of EHEC.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Andre Becker S. Saidenberg, Arnoud H. M. van Vliet, Marc Stegger, Thor Bech Johannesen, Torsten Semmler, Marcos Cunha, Alessandro C. de O. Silveira, Eleine Kuroki Anzai, Isabel C. A. Scaletsky, Anders Dalsgaard, Roberto M. La Ragione, Terezinha Knobl
Summary: Extraintestinal pathogenic Escherichia coli (ExPEC) is a globally distributed pathogen associated with human and companion animal diseases, while avian pathogenic pathotype (APEC) severely impacts poultry health and production. The similarities between APEC and human ExPEC suggest a potential for zoonotic transmission.
VETERINARY MICROBIOLOGY
(2022)
Review
Microbiology
Valerio M. Sora, Gabriele Meroni, Piera A. Martino, Alessio Soggiu, Luigi Bonizzi, Alfonso Zecconi
Summary: The One Health approach highlights the importance of antimicrobial resistance in both public health and food animal production, with specific focus on the ExPEC strains of E. coli which may transfer resistance genes to other pathogenic bacteria, posing a serious threat to public health globally.
Article
Immunology
Jin Hu, Dongfang Wang, Xingfa Huang, Yang Yang, Xin Lian, Wenjun Wang, Xiao Xu, Yulan Liu
Summary: This study explores the role of TolC in the pathogenesis of ExPEC strain PPECC42 and finds that TolC is closely associated with the virulence of ExPEC.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemical Research Methods
Khonaw Kader Salh
Summary: This study investigated the isolation of etiologic agents from patients with urinary tract infections (UTIs) and found that Escherichia coli (E. coli) and Klebsiella pneumoniae (K. pneumoniae) were the most common causative bacteria. It also identified a high incidence of extended-spectrum β-lactamase (ESBL) genes and recorded high resistance to multiple antibiotics. The study emphasizes the importance of formulating effective antibiotic treatment policies in Iraq.
COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING
(2022)
