Article
Chemistry, Organic
Bernard Fernandez, Jean Armengaud, Gilles Subra, Christine Enjalbal
Summary: This study investigates the feasibility of using high energy charge remote fragmentations to analyze the N-terminus of proteins, and compares it with low energy collision-induced dissociations. The results show that the amino acid composition significantly affects the dissociation pathways of peptide backbones.
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
(2022)
Review
Chemistry, Analytical
Hugo M. Santos, Luis B. Carvalho, Carlos Lodeiro, Goncalo Martins, Ines L. Gomes, Wilson D. T. Antunes, Vanessa Correia, Maria M. Almeida-Santos, Helena Rebelo-de-Andrade, Antonio P. A. Matos, J. L. Capelo
Summary: This work presents a tutorial on the capabilities of bioanalytical mass spectrometry in detecting and differentiating viruses at the peptide level, as well as providing diagnosis and prognosis for infected patients. It offers insights into the possibilities of current high-resolution mass spectrometry technology and bioinformatics.
MICROCHEMICAL JOURNAL
(2023)
Article
Microbiology
Gyu Ri Kim, Si Hyun Kim, Eun-Young Kim, Eun Hee Park, In Yeong Hwang, Seok Hoon Jeong, Hyun Soo Kim, Young Ah Kim, Young Uh, Kyeong Seob Shin, Young Ree Kim, Namhee Ryoo, Jong Hee Shin, Jeong Hwan Shin
Summary: This study evaluated the usefulness of MALDI-TOF VITEK MS for Salmonella identification. The results showed that VITEK MS could accurately identify most Salmonella species, but had lower accuracy in identifying typhoidal Salmonella and Salmonella enterica ssp. diarizonae isolates.
Review
Chemistry, Analytical
Judy Gopal, Manikandan Muthu
Summary: Mushrooms have transitioned from a luxury food to a widely accepted nutrient-rich food. Mushroom polysaccharides have become icons of mushroom bioactivity, and the analytical techniques for their analysis have been consolidated and summarized.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Sabarinath Peruvemba Subramanian, Vairavan Lakshmanan, Dasaradhi Palakodeti, Ramaswamy Subramanian
Summary: This study characterized the glycome and transcriptome of mucin-type O-glycans in planarian S. mediterranea using mass spectrometry and in silico approaches. The results revealed the composition, structure, and related genes involved in mucin-type O-glycan biosynthesis in planaria. The study highlighted the unique temporal and spatial role of mucin-type O-glycans during embryogenesis, organogenesis, and in adulthood.
Article
Microbiology
Camila Morschbacher Wilhelm, Maiara dos Santos Carneiro, Everton Inamine, Afonso Luis Barth
Summary: This study aimed to evaluate an adaptation of the MBT-ASTRA technique for faster antimicrobial susceptibility testing. The adapted method showed high categorical agreement with the standard method, indicating its potential for rapid AST results.
MICROBIOLOGY SPECTRUM
(2023)
Review
Chemistry, Applied
Mariangela Valletta, Sara Ragucci, Nicola Landi, Antimo Di Maro, Paolo Vincenzo Pedone, Rosita Russo, Angela Chambery
Summary: Mass spectrometry (MS) methodologies are increasingly used in food authentication and traceability due to their unparalleled specificity, accuracy, and sensitivity. Protein and peptide profiling using MS has become more consolidated and is being utilized to assess food traceability and authenticity. Recent applications of MS in protein/peptide analyses in food matrices show potential in ensuring the quality of agro-food products.
Article
Microbiology
Karl Dichtl, Isabel Klugherz, Hanna Greimel, Josefa Luxner, Julian Koeberl, Simone Friedl, Ivo Steinmetz, Eva Leitner
Summary: This study compared the performance of MALDI Biotyper, EXS2600, and Vitek MS, three commonly used MALDI-TOF MS systems, in testing difficult-to-identify bacteria. The results showed high valid result rates and agreement with sequencing data for all three systems, and low misidentification rates for the majority of isolates.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Chemistry, Applied
Qiuxia Yang, Yushan Guo, Yueming Jiang, Bao Yang
Summary: Oligosaccharides are recognized as prebiotics and their structure is closely related to bioactivity. In this study, UPLC-MS/MS was developed to identify the structure of oligosaccharides, which mainly appeared as sodium adduct in the chromatogram. The fragmentation patterns of oligosaccharides were summarized for different glycosidic linkages. The results showed that UPLC-MS/MS was effective in identifying the structure of oligosaccharides and could be applied in polysaccharide structure identification.
FOOD HYDROCOLLOIDS
(2023)
Article
Multidisciplinary Sciences
Kristine Korzow Richter, Maria C. Codlin, Melina Seabrook, Christina Warinner
Summary: Collagen peptide mass fingerprinting by MALDI-TOF-MS, also known as ZooMS, is a rapidly growing analytical technique in the fields of archaeology, ecology, and cultural heritage. It enables rapid taxonomic identification of large bone assemblages and cultural heritage objects. However, there are limitations that need to be addressed for future advancements.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Analytical
Jia Yi, Yueqing Shen, Yi Yang, Chengpin Shen, Baohong Liu, Liang Qiao, Yan Wang
Summary: By characterizing gingival crevicular fluid (GCF) sediments and identifying potential protein biomarkers using mass spectrometry techniques, this study has contributed to the early diagnosis of periodontitis and understanding of its pathogenesis. The identified biomarkers hold potential value for accurate monitoring and can be utilized by non-specialists for widespread screening.
Article
Biology
Marcelo de M. A. Coura, Eder A. Barbosa, Guilherme D. Brand, Carlos Bloch, Joao B. de Sousa
Summary: The incidence of colorectal cancer (CRC) is increasing in Brazil and there is a lack of reliable biomarkers for diagnosis and prognosis. Alterations in N-glycosylation profiles in serum and tissues of colon cancer patients were identified using mass spectrometry technologies. While serum N-glycans were mostly decreased, certain forms were overexpressed in tumor tissues. There was little correlation between tissue and serum N-glycosylation profiles in cancer patients. Total serum N-glycome analysis showed potential in discriminating patients from healthy controls, but compositions in serum did not parallel those found in tumor tissues.
Article
Chemistry, Analytical
Hernando Rosales-Solano, Victor Galievsky, Khaled Murtada, Pavle Radovanovic, Janusz Pawliszyn
Summary: In this study, a novel on-site approach for detecting and differentiating enriched unsaturated lipids was proposed by coupling SPME probes with Raman spectroscopy. Results showed that the C18/PAN coating exhibited less interference when analyzing unsaturated lipids, and Raman spectroscopy was found to be particularly useful for analyzing lipid unsaturation. The developed protocol demonstrated great SPME activity and high detection ability for various unsaturated lipids in different complex matrixes.
ANALYTICAL CHEMISTRY
(2022)
Article
Veterinary Sciences
Matteo Cuccato, Sara Divari, Paola Sacchi, Flavia Girolami, Francesca Tiziana Cannizzo
Summary: This study applied MALDI-TOF mass spectrometry to analyze milk samples from cows with different SCC, and found significant differences in polypeptide/protein profiles between the two groups. Classification algorithms were used to classify new spectra into the two classes, supporting MALDI-TOF mass spectrometry profiling as a tool to detect mastitic milk samples.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Food Science & Technology
Omotade Richard Ogunremi, Susette Freimueller Leischtfeld, Susanne Miescher Schwenninger
Summary: MALDI-TOF MS combined with 16S rRNA gene sequencing was used to identify LAB species composition in Kunu-zaki, and it was found that certain strains of LAB are capable of producing dextran and/or fructan, which can influence the rheological properties of Kunu-zaki.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Medical Laboratory Technology
Philipp Schuetz
Summary: PCT can help differentiate bacterial from non-bacterial infections and inflammation states, particularly in respiratory illness. PCT-guided antibiotic stewardship has been shown to reduce antibiotic exposure and associated side-effects in patients with respiratory infection and sepsis. Patient-specific decisions regarding antibiotic usage should consider clinical situation, pretest probability for bacterial infection, severity of presentation, and PCT test results.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Health Care Sciences & Services
Christoph Becker, Sebastian Gross, Martina Gamp, Katharina Beck, Simon A. Amacher, Jonas Mueller, Chantal Bohren, Rene Blatter, Rainer Schaefert, Philipp Schuetz, Joerg Leuppi, Stefano Bassetti, Sabina Hunziker
Summary: This study investigated the association between patients' decisional control preference (DCP) and their medical knowledge, ward round performance measures, and perceived quality of care measures. The findings demonstrate that patients with an active DCP have lower trust in physicians and nurses and are less satisfied with their hospital stay.
JOURNAL OF GENERAL INTERNAL MEDICINE
(2023)
Letter
Nutrition & Dietetics
Carla Gressies, Nina Kaegi-Braun, Filomena Gomes, Philipp Schuetz
CLINICAL NUTRITION
(2023)
Article
Geriatrics & Gerontology
Umberto Spennato, Nathalie Lerjen, Jennifer Siegwart, Beat Mueller, Philipp Schuetz, Daniel Koch, Tristan Struja
Summary: This retrospective cohort study investigated the prevalence of physical restraints and their associated outcomes in medical wards of a tertiary care hospital. The results showed that sensor mats were the most frequently used restraint, followed by blanket restrictions, bedrails, and belts. Patients with restraints had longer hospital stays, higher rates of falls, and increased mortality.
Article
Endocrinology & Metabolism
Natasha Anouschka Mueller, Nina Kaegi-Braun, Mirsada Durmisi, Carla Gressies, Pascal Tribolet, Zeno Stanga, Beat Mueller, Philipp Schuetz
Summary: During illness, the conversion of thyroxine (T4) to triiodothyronine (T3) is reduced, known as low T3 syndrome. This study aimed to investigate the prognostic role of low T3 syndrome in patients at nutritional risk, including mortality, clinical outcomes, and response to nutritional support.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2023)
Review
Nutrition & Dietetics
Carla Gressies, Pascal Tribolet, Philipp Schuetz
Summary: Disease-related malnutrition in patients in the general medical ward is a complex syndrome that affects recovery and increases morbidity and mortality. Recent advances in research have led to better understanding of diagnostic criteria and evidence-based treatment for malnutrition. More specific clinical parameters and blood biomarkers are needed to personalize treatments. There is also a need for improved education and screening for nutrition risk in hospitals.
JOURNAL OF PARENTERAL AND ENTERAL NUTRITION
(2023)
Article
Nutrition & Dietetics
Leonie Mueller, Nicole Mentil, Nathalie Staub, Stephanie Griot, Tobias Olpe, Felice Burn, Sebastian Schindera, Beat Mueller, Philipp Schuetz, Zeno Stanga, Annic Baumgartner
Summary: This study examined the association of skeletal muscle index (SMI) at the thoracic level with nutritional and clinical outcomes and response to nutritional intervention. The results showed that SMI at the thoracic vertebra provided limited prognostic information regarding clinical outcomes and nutritional treatment response.
Article
Geriatrics & Gerontology
Jennifer Siegwart, Umberto Spennato, Nathalie Lerjen, Beat Mueller, Philipp Schuetz, Daniel Koch, Tristan Struja
Summary: A retrospective cohort study in a Swiss tertiary care hospital assessed the predictive ability of different clinical scores for fall risk and developed a new fall risk score. The scores showed fair accuracy in predicting fall risk, which could help in establishing preventive strategies.
Article
Microbiology
Nicole E. Putnam, Jung-Ho Youn, Meghan A. Wallace, Paul M. Luethy, Carey-Ann D. Burnham, Susan Butler-Wu, John P. Dekker, Anna F. Lau
Summary: The Streptococcus bovis group consists of seven species and subspecies, and definitive identification is important due to their association with various diseases. However, currently used identification platforms have limitations and inconsistencies.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Nutrition & Dietetics
Roxana Wimmer, Andrea Audetat, Julia Binggeli, Philipp Schuetz, Nina Kaegi-Braun
Summary: A study conducted in the Swiss population found that a significant proportion of individuals have low energy and protein intake. Factors such as high income, obesity, and living in households with children were associated with a reduced risk of low energy intake, while being in the age group of 65-75 years and being female were associated with a higher risk of low protein intake. Regular meat consumption was found to reduce the risk of low protein intake.
Review
Nutrition & Dietetics
Franziska Stumpf, Bettina Keller, Carla Gressies, Philipp Schuetz
Summary: The interplay between inflammation and nutrition has attracted significant attention recently. Inflammation has been identified as a critical factor in disease-related malnutrition, leading to anorexia, reduced food intake, muscle catabolism, and insulin resistance. Inflammation also affects the response to nutritional treatment, with patients with high inflammation showing no response and patients with lower inflammation levels showing a response. This may explain the conflicting results of nutritional trials.
Article
Biochemistry & Molecular Biology
Fabienne Jaun, Maria Boesing, Giorgia Luethi-Corridori, Kristin Abig, Nando Bloch, Stephanie Giezendanner, Victoria Grillmayr, Philippe Haas, Anne B. Leuppi-Taegtmeyer, Juergen Muser, Andrea Raess, Philipp Schuetz, Michael Brandle, Joerg D. Leuppi
Summary: The role of vitamin D in the COVID-19 pandemic has been controversial, and the effectiveness of vitamin D-3 supplementation in COVID-19 patients remains inconclusive. This study conducted a randomized, placebo-controlled double-blind trial on hospitalized COVID-19 patients to compare the effect of high-dose vitamin D-3 supplementation on length of hospital stay. The results showed that vitamin D-3 supplementation did not significantly shorten the hospital stay.
Editorial Material
Nutrition & Dietetics
Philipp Schuetz
JOURNAL OF PARENTERAL AND ENTERAL NUTRITION
(2023)
Article
Medicine, General & Internal
Giorgia Luethi-Corridori, Maria Boesing, Nicola Ottensarendt, Anne Barbara Leuppi-Taegtmeyer, Philipp Schuetz, Joerg Daniel Leuppi
Summary: This study aimed to identify predictors of length of hospital stay, mortality, and rehospitalization in patients admitted for acute exacerbation of COPD. The results showed that the need for oxygen supplementation was the only significant predictor of hospital stay, while older age, COPD severity, active cancer, and arrhythmias were associated with higher mortality.
JOURNAL OF CLINICAL MEDICINE
(2023)
Review
Geriatrics & Gerontology
Marco Falcone, Michael Bauer, Ricard Ferrer, Gaetan Gavazzi, Juan Gonzalez del Castillo, Alberto Pilotto, Philipp Schuetz
Summary: The optimal treatment of infections in the elderly population is challenging due to less specific clinical symptoms and signs. Elderly patients have a weaker immune response to infection and may be at risk of both over- and undertreatment. The use of biomarkers, particularly procalcitonin (PCT), has shown potential in assessing the risk of septic complications and guiding antibiotic treatment decisions in elderly patients.
AGING CLINICAL AND EXPERIMENTAL RESEARCH
(2023)
