期刊
CLINICAL IMMUNOLOGY
卷 145, 期 1, 页码 1-10出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.clim.2012.07.004
关键词
Regulatory B cells; Regulatory T cells; Interleukin-10; Peripheral tolerance; Autoimmunity
类别
资金
- Danish Rheumatism Association
- A.P. Moller's Foundation
- Copenhagen University Hospital Research Fund
- Novo Nordisk Foundation
Human B cells are able to secrete IL-10 after stimulation with mitogens, but their ability to produce IL-10 and regulate T-cell responses after stimulation with self-antigens is unclear. We co-cultured thyroglobulin-pulsed B cells from healthy donors with autologous T cells and observed production of IL-10 and TGF-beta, in addition to TNF-alpha and IL-6. Pulsing with foreign antigen, tetanus toxoid (TT), induced a Th1-response with minimal IL-10 production. After thyroglobulin-pulsing, 1.10 +/- 0.50% of B cells and 1.00 +/- 0.20% of CD4(+) T cells produced IL-10, compared to 0.29 +/- 0.19% of B cells (P=0.01) and 0.13 +/- 0.15% of CD4(+) T cells (P=0.006) following TT-pulsing. Thyroglobulin-stimulated, IL-10-secreting B cells were enriched within CD5(+) and CD24(high) cells. While thyroglobulin-pulsed B cells induced only modest proliferation of CD4(+) T cells, B cells pulsed with TT induced vigorous proliferation. Thus, B cells mediate self-antigen-specific IL-10, TNF-alpha and IL-6 production in co-cultures with T cells and contribute actively to these cytokine secretions. (c) 2012 Elsevier Inc. All rights reserved.
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