Article
Clinical Neurology
Yanyan Jiang, Jing Chen, Yunchuang Sun, Fan Li, Luhua Wei, Wei Sun, Jianwen Deng, Yun Yuan, Zhaoxia Wang
Summary: The combination of salivary miR-29a-3p and miR-29c-3p shows potential as a diagnostic biomarker for idiopathic PD. The expression level of miR-29a-3p in PD patients was significantly lower than ET patients, but higher than MSA patients.
FRONTIERS IN NEUROLOGY
(2021)
Article
Critical Care Medicine
Scott M. Sutherland, Ahmad Kaddourah, Scott E. Gillespie, Danielle E. Soranno, Robert P. Woroniecki, Rajit K. Basu, Michael Zappitelli
Summary: This study proposed an Acute Kidney Injury Score by combining creatinine and urine output criteria, which more accurately characterizes the severity and outcomes of acute kidney injury. The results showed that higher scores were associated with increased mortality and nonrecovery risks.
CRITICAL CARE MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Lars Erichsen, Chantelle Thimm, Wasco Wruck, Daniela Kaierle, Manon Schless, Laura Huthmann, Thomas Dimski, Detlef Kindgen-Milles, Timo Brandenburger, James Adjaye
Summary: Acute kidney injury (AKI) is a common and serious kidney disease, with poor clinical outcomes. It can be detected by symptoms such as increased serum creatinine and decreased urine output. Identifying cytokines for early detection of AKI is important for preventing its progression to chronic kidney disease (CKD). In this study, the urine of AKI patients was analyzed, and ADIPOQ, EGF, and SERPIN3A were suggested as potential cytokines for early detection of AKI. VEGF, SERPIN3A, TNFSF12, ANPEP, CXCL1, REN, CLU, and PLAU were recommended as common cytokines for detecting AKI in later stages.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Ana F. Louro, Marta A. Paiva, Marta R. Oliveira, Katharina A. Kasper, Paula M. Alves, Patricia Gomes-Alves, Margarida Serra
Summary: This study isolated extracellular vesicles (EV) from key stages of human induced pluripotent stem cell-cardiomyocyte differentiation and identified hiPSC as a promising cell biofactory for EV production. The findings demonstrate that hiPSC-EV have pro-angiogenic and pro-proliferative properties, suggesting their potential in cardiac regenerative applications.
Article
Biology
Nur Syakirah Othman, Amilia Aminuddin, Shahidee Zainal Abidin, Saiful Effendi Syafruddin, Mohd Faizal Ahmad, Norfilza Mohd Mokhtar, Jaya Kumar, Adila A. Hamid, Azizah Ugusman
Summary: Hyperglycemia in diabetes mellitus leads to oxidative stress, apoptosis, and dysfunction of vascular endothelial cells. This study aimed to analyze the miRNA profile of human umbilical-vein endothelial cells (HUVECs) exposed to hyperglycemia. RNA sequencing revealed 17 differentially expressed miRNAs between control and hyperglycemia groups, with 4 upregulated and 13 downregulated miRNAs. Validation with qPCR confirmed the differential expression of two miRNAs. These findings suggest a role for miRNAs in diabetic vascular endothelial dysfunction and provide potential targets for therapy.
Article
Oncology
Yuwei Xie, Yixiu Wang, Weijie Xue, Hao Zou, Kun Li, Kui Liu, Wei Zhao, Chengzhan Zhu, Jingyu Cao
Summary: This study investigated differentially expressed RNA profiles in hepatocellular carcinoma (HCC) and identified several miRNAs, such as Hsa-miR-1269a and Hsa-miR-421, that are associated with the development and prognosis of HCC.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Xinghua Guo, Hongquan Peng, Peijia Liu, Leile Tang, Jia Fang, Chiwa Aoieong, Tou Tou, Tsungyang Tsai, Xun Liu
Summary: Through metabolic profiling, the study identified caffeine metabolism as the most important pathway in the progression of CKD. Caffeine was found to be the most significant metabolite that decreases with the deterioration of the CKD stage.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Oncology
Ling Xue, Wenli Guo, Li Li, Santao Ou, Tingting Zhu, Liang Cai, Wenfei Ding, Weihua Wu
Summary: Heat stroke can lead to systemic inflammatory response, resulting in multi-organ dysfunction including AKI and electrolyte disturbances. The study successfully induced a mouse model of HS and found increased creatinine levels, active metabolism, kidney hemorrhage and apoptosis, upregulation of Aifm2, HMGB1, and RAGE, and enrichment of the biosynthesis of unsaturated fatty acids pathway in response to HS-related AKI. These findings suggest a potential correlation between the HMGB1/RAGE pathway and biosynthesis of unsaturated fatty acids in the progression of HS-related AKI.
MOLECULAR MEDICINE REPORTS
(2021)
Article
Multidisciplinary Sciences
Daniel Morell-Garcia, David Ramos-Chavarino, Josep M. Bauca, Paula Argente del Castillo, Maria Antonieta Ballesteros-Vizoso, Luis Garcia de Guadiana-Romualdo, Cristina Gomez-Cobo, J. Albert Pou, Rocio Amezaga-Menendez, Alberto Alonso-Fernandez, Isabel Llompart, Ana Garcia-Raja
Summary: This study aimed to evaluate the predictive role of urinary parameters in the progression of COVID-19 in hospitalized patients. The results showed that early alterations in urinary and serum parameters are important prognostic factors for AKI, ICU admission, and death in patients with COVID-19.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Hitoshi Iwamoto, Masaaki Okihara, Isao Akashi, Yu Kihara, Osamu Konno, Shigeyuki Kawachi, Makoto Sunamura, Masahiro Sugimoto
Summary: Kidney biopsy is commonly used to diagnose kidney transplant dysfunction. In this study, capillary electrophoresis-mass spectrometry was used to analyze the biofluids of transplant recipients. Metabolomics analyses revealed significant differences in the metabolomic profiles of the different groups, particularly in arginine biosynthesis and purine metabolism in plasma samples. Different types of kidney disorders also showed distinct metabolomic profiles.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Sheng Zhang, Chao Li, Tingting Feng, Shuai Cao, Heng Zhou, Limin Li, Qing Hu, Xiuhong Mao, Shen Ji
Summary: The study investigated metabolic changes in serum and kidney tissues of mice exposed to Realgar, revealing associations with amino acid metabolism, lipid metabolism, glutathione metabolism, and purine metabolism pathways in Realgar nephrotoxicity.
FRONTIERS IN PHARMACOLOGY
(2021)
Review
Medicine, Research & Experimental
Fatemeh Sabet Sarvestani, Negar Azarpira, Ismail H. Al-Abdullah, Ali-Mohammad Tamaddon
Summary: Ischemia reperfusion injury can lead to tissue damage, especially during organ transplantation. By manipulating miRNAs level, they can be used as a biomarker for early diagnosis of IRI or suggestive to be therapeutic agents in clinical situation in future.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Multidisciplinary Sciences
Sarah A. Taylor, Shang-Yang Chen, Gaurav Gadhvi, Liang Feng, Kyle D. Gromer, Hiam Abdala-Valencia, Kiwon Nam, Salina T. Dominguez, Anna B. Montgomery, Paul A. Reyfman, Lorena Ostilla, Joshua B. Wechsler, Carla M. Cuda, Richard M. Green, Harris Perlman, Deborah R. Winter
Summary: This study identified three distinct macrophage populations in pediatric cholestatic liver disease through single-cell RNA sequencing and immunofluorescence, characterized by their transcriptional profiles as lipid-associated macrophages, monocyte-like macrophages, and adaptive macrophages. These cholestatic macrophages showed reduced expression of immune regulatory genes compared to normal hepatic macrophages, indicating potential for targeted therapeutic strategies in reducing cholestatic liver injury.
Article
Biochemistry & Molecular Biology
Ingrid Cifola, Federica Fratini, Beatrice Cardinali, Valentina Palmieri, Giuliana Gatti, Tommaso Selmi, Sara Donzelli, Andrea Sacconi, Valeriana Cesarini, Hany E. Marei, Massimilano Papi, Giovanni Blandino, Carlo Cenciarelli, Germana Falcone, Igea D'Agnano
Summary: Glioblastoma (GBM) is a common and aggressive brain tumor with limited treatment options. The heterogeneity of GBM tumor cells, especially the cancer stem cells (CSCs), plays a crucial role in clinical outcomes. This study explored the role of extracellular vesicles (EVs) in CSCs and GBM tumor cells, finding that the miRNA and protein content of EVs differ, with GBM-sEVs enriched for tumor suppressor miRNAs and oncoproteins, and CSC-sEVs enriched for pro-tumor miRNAs and proteins related to sternness, cell proliferation, and apoptosis.
Article
Biochemistry & Molecular Biology
Xiaoe You, Baochun Guo, Zhen Wang, Hualin Ma, Lixia Liu, Ru Zhou, Yaxuan Zheng, Xinzhou Zhang
Summary: This study used label-free quantitative LC-MS-MS proteomics and un-targeted metabolomics to investigate the protein and metabolite changes in the urine of renal anemia patients before and after Roxadustat therapy. Results showed that Roxadustat treatment significantly altered 46 proteins (including 15 upregulated and 31 downregulated proteins) and 207 metabolites in urine samples. The altered proteins were validated by PRM. Proteomics and metabolomics analysis revealed that Roxadustat could modulate protein expression and reverse potential metabolic changes, exerting hypotensive, lipid metabolic regulation, and renoprotective effects in clinical practice.
Review
Medical Laboratory Technology
Janani Saikumar, Krithika Ramachandran, Vishal S. Vaidya
CLINICAL CHEMISTRY
(2014)
Review
Biochemistry & Molecular Biology
Sarah Campion, Jiri Aubrecht, Kim Boekelheide, David W. Brewster, Vishal S. Vaidya, Linnea Anderson, Deborah Burt, Edward Dere, Kathleen Hwang, Sara Pacheco, Janani Saikumar, Shelli Schomaker, Mark Sigman, Federico Goodsaid
EXPERT OPINION ON DRUG METABOLISM & TOXICOLOGY
(2013)
Article
Multidisciplinary Sciences
Amrendra Kumar Ajay, Janani Saikumar, Vanesa Bijol, Vishal S. Vaidya
Article
Toxicology
Janani Saikumar, Dana Hoffmann, Tae-Min Kim, Victoria Ramirez Gonzalez, Qin Zhang, Peter L. Goering, Ronald P. Brown, Vanesa Bijol, Peter J. Park, Sushrut S. Waikar, Vishal S. Vaidya
TOXICOLOGICAL SCIENCES
(2012)
Article
Toxicology
Kathryn L. Pellegrini, Tao Han, Vanesa Bijol, Janani Saikumar, Florin L. Craciun, William W. Chen, James C. Fuscoe, Vishal S. Vaidya
TOXICOLOGICAL SCIENCES
(2014)
Article
Multidisciplinary Sciences
Janani Saikumar, China N. Byrns, Matthew Hemphill, David F. Meaney, Nancy M. Bonini
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Biochemical Research Methods
Janani Saikumar, Joshua Kim, China N. Byrns, Matthew Hemphill, David F. Meaney, Nancy M. Bonini
Summary: Researchers have developed a controlled Drosophila TBI (dTBI) device that can inflict head injury on flies, mimicking human TBI with significant similarity. This device can be utilized for genetic or pharmacological screens to identify modifiers of injury.
Article
Cell Biology
China N. N. Byrns, Janani Saikumar, Nancy M. M. Bonini
Summary: The authors demonstrate that glial AP1 is initially protective after TBI but can lead to tau pathology and degeneration over time. This process may also occur with normal aging, suggesting that TBI could accelerate age-related degenerative processes.
Article
Physiology
Florin L. Craciun, Amrendra K. Ajay, Dana Hoffmann, Janani Saikumar, Steven L. Fabian, Vanesa Bijol, Benjamin D. Humphreys, Vishal S. Vaidya
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2014)