Review
Environmental Sciences
Mohammad Y. Ashfaq, Dana A. Da'na, Mohammad A. Al-Ghouti
Summary: Bacteria play diverse roles in the environment, with MALDI-TOF MS technology gaining attention for routine bacterial identification. This technique has been applied in various environmental studies, showing benefits in rapid and cost-effective bacteria identification.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2022)
Article
Microbiology
Adriana Calderaro, Mirko Buttrini, Monica Martinelli, Benedetta Farina, Tiziano Moro, Sara Montecchini, Maria Cristina Arcangeletti, Carlo Chezzi, Flora De Conto
Summary: This study evaluated a Matrix-Assisted Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS (T-MALDI)) method for rapid classification of circulating C. difficile strains. The results showed that this method has good recognition and validation capabilities, especially for PR1-PR5 ribotyping.
Article
Biochemistry & Molecular Biology
Michal Zloch, Ewelina Maslak, Wojciech Kupczyk, Marek Jackowski, Pawel Pomastowski, Boguslaw Buszewski
Summary: This study evaluated the use of different culture media in reflecting the microbial compositions of diabetic foot infections in Poland. The results showed significant differences in species coverage and efficiency between the investigated media. Selecting the appropriate media set can significantly improve the accuracy of reflecting the microbial compositions of diabetic foot infections.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Romain Lotte, Audrey Courdurie, Alice Gaudart, Audrey Emery, Alicia Chevalier, Albert Tran, Mathilde Payen, Raymond Ruimy
Summary: Direct identification of bacteria in ascitic fluid can improve the management of patients with spontaneous bacterial peritonitis by facilitating early introduction of appropriate antibiotic therapy.
Review
Chemistry, Analytical
Hugo M. Santos, Luis B. Carvalho, Carlos Lodeiro, Goncalo Martins, Ines L. Gomes, Wilson D. T. Antunes, Vanessa Correia, Maria M. Almeida-Santos, Helena Rebelo-de-Andrade, Antonio P. A. Matos, J. L. Capelo
Summary: This work presents a tutorial on the capabilities of bioanalytical mass spectrometry in detecting and differentiating viruses at the peptide level, as well as providing diagnosis and prognosis for infected patients. It offers insights into the possibilities of current high-resolution mass spectrometry technology and bioinformatics.
MICROCHEMICAL JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Xia Wang, Guanqi Wu, Shuo Han, Jingjing Yang, Xiangwei He, Haifang Li
Summary: Plant endophytic bacteria play important roles in plant growth and stress resistance. This study identified and classified endophytic bacteria in Populus based on MALDI-TOF MS data and 16S rRNA gene sequencing, and verified the classification capacity of protein mass fingerprints. The results provide a valuable reference for differentiating and identifying endophytic bacteria.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Organic
Bernard Fernandez, Jean Armengaud, Gilles Subra, Christine Enjalbal
Summary: This study investigates the feasibility of using high energy charge remote fragmentations to analyze the N-terminus of proteins, and compares it with low energy collision-induced dissociations. The results show that the amino acid composition significantly affects the dissociation pathways of peptide backbones.
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
(2022)
Article
Food Science & Technology
Sung-Youn Kim, Sol Yi Park, Ji-Eun Jin, Kyong-Suk Hong, Dae-Jung Kim, Yong-Kyoung Kim, Hee-Chang Shin, Soon-Kil Cho, Dong-Hyun Kang
Summary: This study compared three different methods for the identification of six LAB species and found that species-specific PCR and MALDI-TOF MS were highly accurate and suitable for LAB identification.
JOURNAL OF FOOD SCIENCE
(2022)
Article
Multidisciplinary Sciences
Natalija Topic Popovic, Slavko Kepec, Snjezana P. Kazazic, Ivancica Strunjak-Perovic, Krunoslav Bojanic, Rozelindra Coz-Rakovac
Summary: This study evaluated the use of the API20E biochemical identification system as a supportive tool for enhancing the discrimination of environmental bacteria by the MALDI-TOF MS method. The results showed that the API20E system can effectively identify bacterial genera, but there are issues with misidentification. In environmental isolates with lower identification scores, the API20E tests can be used as a purposeful and inexpensive identification support.
Article
Biotechnology & Applied Microbiology
Samart Dorn-In, Sirkka Mang, Karin Schwaiger
Summary: This study investigated cold-tolerant Clostridium spp. strains isolated from various samples, identifying them into different groups based on genetic and biochemical characteristics. Spiked beef samples showed changes in odor after storage, while maintaining similar color and meat quality compared to the control group.
Article
Microbiology
Gyu Ri Kim, Si Hyun Kim, Eun-Young Kim, Eun Hee Park, In Yeong Hwang, Seok Hoon Jeong, Hyun Soo Kim, Young Ah Kim, Young Uh, Kyeong Seob Shin, Young Ree Kim, Namhee Ryoo, Jong Hee Shin, Jeong Hwan Shin
Summary: This study evaluated the usefulness of MALDI-TOF VITEK MS for Salmonella identification. The results showed that VITEK MS could accurately identify most Salmonella species, but had lower accuracy in identifying typhoidal Salmonella and Salmonella enterica ssp. diarizonae isolates.
Article
Microbiology
Camila Morschbacher Wilhelm, Maiara dos Santos Carneiro, Everton Inamine, Afonso Luis Barth
Summary: This study aimed to evaluate an adaptation of the MBT-ASTRA technique for faster antimicrobial susceptibility testing. The adapted method showed high categorical agreement with the standard method, indicating its potential for rapid AST results.
MICROBIOLOGY SPECTRUM
(2023)
Article
Chemistry, Analytical
Jiaojiao Sun, Haimei Shi, Yuyan Xue, Wenmin Cheng, Menghuan Yu, Chuanfan Ding, Fuxing Xu, Shaoning Yu
Summary: The accuracy of bacterial identification based on MALDI-TOF MS can be affected by functional proteins, releasing bacteria from functional material can increase accuracy.
Review
Chemistry, Analytical
Judy Gopal, Manikandan Muthu
Summary: Mushrooms have transitioned from a luxury food to a widely accepted nutrient-rich food. Mushroom polysaccharides have become icons of mushroom bioactivity, and the analytical techniques for their analysis have been consolidated and summarized.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Yuexin Li, Tao Wang, Jianmin Wu
Summary: Fast bacterial identification in urine samples was achieved by capturing bacteria on a microchannel silicon nanowire microfluidic chip, followed by MALDI-TOF MS detection. Bacteria with a concentration of 10(6) CFU mL(-1) in urine samples could be identified without culture under optimized conditions. Furthermore, after 4 hours of culture, bacteria with a concentration as low as 10(3) CFU mL(-1) could be identified.
Article
Infectious Diseases
Aline Cuenod, Martina Aerni, Claudia Bagutti, Banu Bayraktar, Efe Serkan Boz, Cynthia Beisert Carneiro, Carlo Casanova, Alix T. Coste, Peter Damborg, Dirk W. van Dam, Mehmet Demirci, Pavel Drevinek, Olivier Dubuis, Jose Fernandez, Gilbert Greub, Jaroslav Hrabak, Gulen Hurkal Yigitler, Jakub Hurych, Thoger Gorm Jensen, Geraldine Jost, Greetje A. Kampinga, Sonja Kittl, Christine Lammens, Claudia Lang, Reto Lienhard, Julie Logan, Carola Maffioli, Ivana Marekovic, Matthias Marschal, Jacob Moran-Gilad, Oliver Nolte, Michael Oberle, Michael Pedersen, Valentin Pfluger, Sigrid Pranghofer, Julia Reichl, Rob J. Rentenaar, Arnaud Riat, Belen Rodriguez-Sanchez, Camille Schilt, Ann-Kathrin Schlotterbeck, Jacques Schrenzel, Shani Troib, Elise Willems, Mandy Wootton, Dominik Ziegler, Adrian Egli
Summary: The study assessed the mass spectral quality (MSQ) of MALDI-TOF MS and identified simple workflow adaptations to improve it. The intervention of providing feedback and instructions on acquiring MALDI-TOF mass spectra showed improvements in MSQs.
CLINICAL MICROBIOLOGY AND INFECTION
(2023)
Review
Nursing
Tarsila Vieceli, Sofia Tejada, Raquel Martinez-Reviejo, Tomas Pumarola, Jacques Schrenzel, Grant W. Waterer, Jordi Rello
Summary: This review summarizes the evidence of the impact of air pollution on the respiratory microbiome. It suggests that air pollution can alter the composition of respiratory microbiota, increase the colonization of pathogenic microorganisms, and contribute to the development of respiratory infections with antimicrobial resistance.
INTENSIVE AND CRITICAL CARE NURSING
(2023)
Article
Infectious Diseases
Tomefa E. Asempa, April M. Bobenchik, Lori Bourassa, Andrew E. Clark, Morgan T. Hatch, Holly K. Huse, Isabella W. Martin, Brian Mochon, Erik Munson, Maroun M. Sfeir, Monica Srodon, Yungchou Wang, David P. Nicolau
Summary: In 2022, a total of 618 Enterobacterales isolates were collected from outpatient and community settings in the U.S. The susceptibility of these isolates to tebipenem and other antibiotics was tested. The results showed that 90.3% of the isolates had a tebipenem MIC of <= 0.125 mg/L, indicating its potential effectiveness against multidrug-resistant strains.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Immunology
Mikael de Lorenzi-Tognon, Etienne Ruppe, Jacques Schrenzel
MICROBES AND INFECTION
(2023)
Review
Biochemistry & Molecular Biology
Patrice Francois, Jacques Schrenzel, Friedrich Goetz
Summary: Despite advances in medical procedures, staphylococci remain major pathogens causing disease, especially in patients with indwelling catheters and prosthetic devices. Biofilms produced by staphylococci increase resistance to treatment and host immunity. This review explores the composition and regulation of biofilm development, as well as its clinical significance. Recent studies on destroying biofilms as a therapeutic strategy are also discussed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Adele Rakotonirina, Tatiana Galperine, Maxime Audry, Marie Kroemer, Aurelie Baliff, Laurent Carrez, Farshid Sadeghipour, Jacques Schrenzel, Benoit Guery, Eric Allemann
Summary: Clostridioides difficile infection (CDI) is a severe nosocomial infection with a high mortality rate. Although antibiotic treatment is the standard of care, the relapse rate is high and less effective against recurrent infection. Fecal microbiota transplantation (FMT) is a recommended treatment with a high efficacy. The formulation of diluted donor stool needs innovation, and the administration routes should be optimized.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Public, Environmental & Occupational Health
N. Hassoun-Kheir, N. Buetti, V. Olivier, M. Perez, J. Frossard, G. Renzi, J. Schrenzel, P. Saudan, S. Harbarth
Summary: This study investigated the mupirocin resistance in haemo-dialysis patients and found that it was infrequent in both blood and colonizing Staphylococcus aureus isolates. Moreover, a decreasing trend in the annual rate of S. aureus bloodstream infections was observed. Therefore, targeted mupirocin-based decolonization is a sustainable measure for preventing healthcare-associated infections in S. aureus carriers undergoing haemodialysis.
JOURNAL OF HOSPITAL INFECTION
(2023)
Article
Biochemistry & Molecular Biology
Sravya Kovvali, Yuan Gao, Austin Cool, Steffen Lindert, Vicki H. Wysocki, Charles E. Bell, Venkat Gopalan
Summary: Amadori rearrangement products are stable sugar-amino acid conjugates that are formed nonenzymatically during food processing. Understanding bacterial utilization of these fructosamines is crucial due to their impact on the animal gut microbiome. In this study, the catalytic mechanism of the deglycase enzyme FrlB was elucidated through crystallography, computational docking, biochemical assays, and native mass spectrometry. The findings provide insights into the structure-function relationship and mechanisms of enzymes.
Review
Clinical Neurology
E. Berthouzoz, V. Lazarevic, A. Zekeridou, M. Castro, I. Debove, S. Aybek, J. Schrenzel, P. R. Burkhard, V. Fleury
Summary: Parkinson's disease (PD) is associated with dysbiosis of the microbiota, which can influence the development of symptoms and lead to metabolic changes and inflammation. Dysbiosis also decreases the effectiveness of dopaminergic treatments. Analysis of dysbiosis can serve as a biomarker of PD, and interventions modulating the gut microbiota may influence the course of the disease.
REVUE NEUROLOGIQUE
(2023)
Article
Chemistry, Multidisciplinary
Thomas Walker, He Mirabel Sun, Tiffany Gunnels, Vicki Wysocki, Arthur Laganowsky, Hays Rye, David Russell
Summary: Variable-temperature electrospray ionization (vT-ESI) native mass spectrometry (nMS) is used to investigate the thermodynamics of ATP binding to the GroEL tetradecamer chaperonin complex. The study reveals strong enthalpy-entropy compensation (EEC) and variations in stepwise free energy changes, which are consistent with the established nested cooperativity model of GroEL-ATP interactions. The entropy-driven ATP binding is attributed to ligand-induced conformational changes and reorganization of water molecules within the GroEL complex.
ACS CENTRAL SCIENCE
(2023)
Article
Microbiology
Florian Mauffrey, Nadia Gaia, Chiraz Chaabane, Gesuele Renzi, Adrien Fischer, Abdessalam Cherkaoui, Diane Bandeira, Jacques Schrenzel, Vladimir Lazarevic
Summary: We present the genome of a Neisseria meningitidis strain (GE-156) isolated from a bacteremia patient in Switzerland. This strain belongs to a rare mixed serogroup W/Y and sequence type 11847 (clonal complex 167), identified through both routine laboratory examination and genomic sequencing.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Letter
Critical Care Medicine
Sylvain Meyer, Nadia Gaia, Vladimir Lazarevic, Jacques Schrenzel, Bruno Francois, Olivier Barraud
INTENSIVE CARE MEDICINE EXPERIMENTAL
(2023)
Editorial Material
Infectious Diseases
Aude Nguyen, Ludovica Ferrero, Vladimir Lazarevic, Nadia Gaia, Begona Martinez de Tejada, Jacques Schrenzel, Nadia Berkane
Summary: This article presents the first case of postpartum septic arthritis of the pubic symphysis caused by Sneathia sanguinegens, Sneathia vaginalis, and Mageeibacillus indolicus, identified using next generation sequencing.
NEW MICROBES AND NEW INFECTIONS
(2023)
Article
Biochemical Research Methods
Mengxuan Jia, Yang Song, Chen Du, Vicki H. Wysocki
Summary: The charge partitioning during the dissociation of protein complexes in the gas phase is influenced by various factors, including interfacial interactions, protein flexibility, protein conformation, and dissociation methods. This study used two cysteine-containing homodimer proteins to investigate the charge partitioning behaviors of collision-induced dissociation (CID) and surface-induced dissociation (SID) processes. Results showed that restructuring dominates with CID, while dissociation with symmetric charge partitioning dominates with SID, regardless of the oxidation state of the intramolecular disulfide bonds. Gas collisions reveal the charge-dependent restructuring/unfolding properties of the protein precursor, while surface collisions predominantly lead to more charge-symmetric monomer separation.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Editorial Material
Biochemical Research Methods
Jenny Brodbelt, Vicki Wysocki
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)