Article
Engineering, Electrical & Electronic
David C. Ferrier, Janice Kiely, Richard Luxton
Summary: This study presents an enzyme-based electrochemical biosensor for detecting propofol concentrations in total intravenous anesthesia. The sensor demonstrates high sensitivity and good specificity, making it a promising tool for real-time monitoring during the maintenance phase of anesthesia.
IEEE SENSORS JOURNAL
(2021)
Article
Chemistry, Analytical
Xiaoyan Gan, Feng Qiu, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: An electrochemical assay for ACP in human serum samples using DNA extension and magnetic bead technologies on rGO-modified electrode was developed, providing high sensitivity and selectivity with a wide detection range and low detection limit.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Lu Fang, Hangxu Ren, Xiyu Mao, Shanshan Zhang, Yu Cai, Shiyi Xu, Yi Zhang, Lihua Li, Xuesong Ye, Bo Liang
Summary: L-Dopa is an effective therapy for Parkinson's disease, but its short half-life and narrow therapeutic window may lead to side effects. Monitoring the concentration of L-Dopa using wearable biosensors is significant to reduce complications. However, the high concentration of interferents in the body poses challenges. A minimal-invasive L-Dopa biosensor based on a flexible differential microneedle array (FDMA) is proposed to address this. The sensor exhibits excellent anti-interference performance and accuracy.
Article
Biochemical Research Methods
Fabio A. Kraft, Holger Baur, Moritz Bommer, Andreas Latz, Stefanie Fitschen-Oestern, Sabine Fuchs, Martina Gerken
Summary: Direct detection of biomarkers from unpurified whole blood using label-free detection platforms has been a challenge. In this study, a wavelength selecting concept based on photonic crystal slabs (PCS) is proposed to address the technical limitations and enable label-free point-of-care biosensing with unfiltered whole blood. The researchers achieved a limit of detection of 3.4 E-4 refractive index units (RIU) for bulk refractive index changes and demonstrated multiplex detection of different immobilization entities. The ability to detect immunoglobulins G (IgG) from unfiltered whole blood was also demonstrated.
BIOMEDICAL OPTICS EXPRESS
(2023)
Article
Biophysics
Kouta Takeda, Ryo Kusuoka, Misaki Inukai, Kiyohiko Igarashi, Hiroyuki Ohno, Nobuhumi Nakamura
Summary: An amperometric L-fucose biosensor was developed using direct electron transfer bioelectrocatalysis, which allowed for accurate detection of L-fucose while eliminating interference from other electroactive compounds.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Mimi Sun, Xinyi Pei, Tong Xin, Jian Liu, Chongbo Ma, Mengzhu Cao, Ming Zhou
Summary: This article demonstrates a fully integrated nanoelectronic system that combines a flexible and disposable microfluidic enzymatic biosensor with a handheld wireless electronic device to monitor glucose in various raw noninvasive biofluids. The system shows potential utility in noninvasive glucose management for both healthy individuals and diabetic patients, and has the capability to expand to the monitoring of other biomolecules.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Lukasz Oldak, Zenon Lukaszewski, Anna Lesniewska, Ksawery Golawski, Piotr Laudanski, Ewa Gorodkiewicz
Summary: A new method for determining the concentration of CDH12, an adhesive protein that plays a significant role in the development, growth, and movement of cancer cells, was developed using surface plasmon resonance imaging. The method demonstrated good selectivity and accuracy, making it suitable for quantitative analysis of human fluid samples.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Electrochemistry
Yanzhen Jing, Shwu Jen Chang, Ching-Jung Chen, Jen-Tsai Liu
Summary: Blood glucose monitoring is crucial for diabetes management, and optical and electrochemical glucose sensors are currently the mainstream technologies. This article introduces the history and advantages/disadvantages of these sensors, and reviews the research progress and challenges of continuous glucose monitoring technology. The study finds that different optical techniques have limitations that hinder the development of optical CGM, while electrochemical glucose sensors are more feasible and reliable for implementing CGM.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2022)
Article
Chemistry, Applied
Muhammad Ihda H. L. Zein, Ari Hardianto, Irkham Irkham, Salma Nur Zakiyyah, Melania Janisha Devi, Ninie Suhana Abdul Manan, Abdullahi Umar Ibrahim, Yeni Wahyuni Hartati
Summary: The technological revolution in agriculture and food has led to the production of genetically modified crops that are high yield and less susceptible to pests and weather conditions. However, safety issues, including antibiotic residues, still persist in the sector. Aptasensors, which are biorecognition molecules, have been developed to detect antibiotics in food with high affinity and specificity.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Article
Endocrinology & Metabolism
Rita Indirli, Emanuele Ferrante, Erica Scalambrino, Eriselda Profka, Marigrazia Clerici, Tommaso Lettera, Andreea Liliana Serban, Walter Vena, Alessandro Pizzocaro, Marco Bonomi, Biagio Cangiano, Giulia Carosi, Gherardo Mazziotti, Luca Persani, Andrea Lania, Maura Arosio, Flora Peyvandi, Giovanna Mantovani, Armando Tripodi
Summary: Klinefelter syndrome patients exhibit a procoagulant imbalance with increased thrombin generation in both platelet-poor plasma and platelet-rich plasma, potentially due to elevated FVIII levels. Higher levels of FVIII, fibrinogen, and FVIII/protein C ratio were observed in KS patients compared to controls.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2021)
Article
Chemistry, Analytical
Yunxiao Feng, Gang Liu, Fan Zhang, Jianwen Liu, Ming La, Ning Xia
Summary: Proteases play a critical role in various physiological processes. A label-free and homogeneous electrochemical method based on the peptide-copper interaction is reported for monitoring protease activity, which has great potential for early diagnosis and treatment of many diseases.
Article
Multidisciplinary Sciences
Mojca Bozic Mijovski, Rickard E. Malmstroem, Nina Vene, Jovan P. Antovic, Alenka Mavri
Summary: The study demonstrates that in vitro addition of idarucizumab to plasma samples from patients increases thrombin generation. Further investigation is needed to explore the use of idarucizumab to neutralize dabigatran in patient plasma samples and the clinical relevance of in vitro increased thrombin generation induced by idarucizumab.
SCIENTIFIC REPORTS
(2021)
Article
Biophysics
Anran Zheng, Wei Zhang, Chao Li, Zhen Guo, Chuanyu Li, Changsong Zhang, Jia Yao, Zhiqi Zhang, Jinze Li, Shasha Zhao, Lianqun Zhou
Summary: A novel HLDT method was developed using a GR-C sensor to quantitatively evaluate heparin potency in whole blood. The method demonstrated high selectivity for heparin potency in 10 μL of whole blood with a detection limit of 0.1 U/mL.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biophysics
Heru Agung Saputra, Jae Heun Chung, Seong Hoon Yoon, Kyeong-Deok Seo, Deog-Su Park, Yoon-Bo Shim
Summary: A disposable amperometric biosensor with a dual monomers-based bioconjugate was developed for the detection of granzyme B (GzmB) and monitoring the cancer progression of patients before and after immunotherapy. The sensor showed high sensitivity and specificity, allowing for monitoring of GzmB levels in patient serum samples.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Cardiac & Cardiovascular Systems
Li Li, Mark Roest, Yaqiu Sang, Jasper A. Remijn, Rob Fijnheer, Karel Smit, Dana Huskens, Jun Wan, Bas de Laat, Joke Konings
Summary: Patients with multiple myeloma have imbalanced blood cell metabolism and WB-TG profile, which may explain the paradoxically high prevalence of bleeding symptoms compared to an increased thrombosis risk. Disturbance is not observed in plasma TG, indicating that blood cells are the major determinants for the imbalanced hemostasis in multiple myeloma patients.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Editorial Material
Hematology
Noemy De Rossi, Lorenzo Alberio, Gregoire Stalder
AMERICAN JOURNAL OF HEMATOLOGY
(2022)
Article
Hematology
Matteo Marchetti, Stefano Barelli, Tobias Gleich, Francisco J. Gomez, Matthew Goodyer, Francesco Grandoni, Lorenzo Alberio
BRITISH JOURNAL OF HAEMATOLOGY
(2022)
Article
Hematology
Luana Rittener-Ruff, Matteo Marchetti, Elena Matthey-Guirao, Francesco Grandoni, Francisco J. Gomez, Lorenzo Alberio
Summary: This study evaluated the diagnostic performance of two rapid immunoassays in detecting heparin-induced thrombocytopenia and compared different diagnostic algorithms. The results showed that the Bayesian approach of sequentially using two rapid immunoassays was most effective in accurately diagnosing heparin-induced thrombocytopenia.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Biology
Vepusha Sathanantham, Lorenzo Alberio, Cedric Bovet, Pierre Fontana, Bernhard Gerber, Lukas Graf, Adriana Mendez, Thomas C. Sauter, Adrian Schmidt, Jan-Dirk Studt, Walter A. Wuillemin, Michael Nagler
Summary: PiCT is proposed as a reliable laboratory test for measuring direct oral anticoagulant drug levels, and it can accurately predict clinically relevant drug concentrations.
Letter
Medicine, General & Internal
Gregoire Stalder, William Reis Da Silva, Amandine Segot, Sabine Blum, Francesco Grandoni, Lorenzo Alberio
EUROPEAN JOURNAL OF INTERNAL MEDICINE
(2023)
Article
Hematology
Charlotte Muret, David Crettaz, Agathe Martin, Alessandro Aliotta, Debora Bertaggia Calderara, Lorenzo Alberio, Michel Prudent
Summary: Two biotinylation methods were developed and compared in this study. The Bio-Direct method was found to be similar to standard PCs in terms of function and phenotype, making it more suitable for use in a blood bank environment.
Review
Medicine, General & Internal
Etienne Revelly, Emmanuelle Scala, Lorenzo Rosner, Valentina Rancati, Ziyad Gunga, Matthias Kirsch, Zied Ltaief, Marco Rusca, Xavier Bechtold, Lorenzo Alberio, Carlo Marcucci
Summary: Heparin-induced thrombocytopenia (HIT) is a severe adverse drug reaction in cardiac surgery involving heparin. It causes immune-mediated thrombocytopenia leading to thrombotic events. Anti-PF4/H antibodies and platelet aggregation tests confirm the diagnosis. Patients with HIT in cardiac surgery face high risks and specific challenges. An institutional protocol has been developed for managing HIT in different phases, including the use of non-heparin anticoagulants and potent antiplatelet drugs.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Cardiac & Cardiovascular Systems
Gregoire Stalder, Antoine Chatte, Noemy De Rossi, Patrick Yerly, Lorenzo Alberio, Eric Eeckhout
Summary: This article describes a rare complication of acute and subacute stent thrombosis, occurring in approximately 1.5% of patients treated with primary percutaneous intervention for ST-elevation myocardial infarction (STEMI). We present a case of a 58-year-old woman who experienced subacute stent thrombosis despite good stent expansion, dual antiplatelet therapy, and anticoagulation. Due to high von Willebrand factor (VWF) levels, N-acetylcysteine was administered to depolymerize VWF, but was not well tolerated. Caplacizumab was then used to prevent VWF from interacting with platelets, resulting in a favorable clinical and angiographic outcome.
EUROPEAN HEART JOURNAL-CASE REPORTS
(2023)
Article
Hematology
Marie Mean, Neal Breakey, Odile Stalder, Lorenzo Alberio, Andreas Limacher, Anne Angelillo-Scherrer, Pierre Fontana, Hans Jurg Beer, Nicolas Rodondi, Drahomir Aujesky, Bernhard Laemmle, Robert Escher
Summary: This study examined the prevalence of laboratory thrombophilic risk factors in elderly patients with VTE and their association with clinical outcomes. The results showed that 78% of patients had at least one risk factor, with elevated levels of various factors being the most common. The study also found significant associations between certain risk factors and VTE recurrence and death.
RESEARCH AND PRACTICE IN THROMBOSIS AND HAEMOSTASIS
(2023)
Letter
Hematology
Mathilde Gavillet, Nathalie Rufer, Francesco Grandoni, Mattia Rizzi, Nicolas Vulliemoz, David Baud, Lorenzo Alberio, Giorgia Canellini, Helene Legardeur
BRITISH JOURNAL OF HAEMATOLOGY
(2023)
Letter
Hematology
Sabine Blum, Mariangela Costanza, Christine Coutaz, Olaia Naveiras, Olivier Spertini, Gerasimos Tsilimidos, Lorenzo Alberio
INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY
(2023)
Article
Hematology
Debora Bertaggia Calderara, Rita Marchi Cappelletti, Ana Patricia Batista Mesquita Sauvage, Stephane Durual, Francisco J. Gomez, Maxime G. Zermatten, Alessandro Aliotta, Alessandro Casini, Lorenzo Alberio
Summary: This study found that global coagulation assays (GCA) can serve as indicators to monitor the biological response to non-factor replacement therapy, such as emicizumab. After treatment, PwHA patients showed improved coagulation activity and fibrin clot composition, although thrombin generation (TG) results remained slightly below the normal reference range. Remarkably, individual patients exhibited varying degrees of response to emicizumab, despite having similar plasma concentrations of the drug.
THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Medicine, General & Internal
Laurence Bamps, Jean-Philippe Armenti, Mirela Bojan, Bruno Grandbastien, Christophe von Garnier, Renaud Du Pasquier, Florian Desgranges, Matthaios Papadimitriou-Olivgeris, Lorenzo Alberio, Martin Preisig, Jurg Schwitter, Benoit Guery
Summary: This study collected health complaints from 474 patients one year after COVID-19 diagnosis and found that age, comorbidity index, and smoking habits were associated with hospital admission. Patients with a history of thromboembolism before SARS-CoV-2 infection had a higher risk of recurrence of thromboembolism at 1 year. Fatigue was the most common neurologic symptom, followed by feeling slowed down, headache, and smell disturbance. COVID-19 patients scored higher in anxiety, depression, fatigue, and sleep measures compared to the healthy population.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Medicine, General & Internal
Annika Burger, Jan-Dirk Studt, Adriana Mendez, Lorenzo Alberio, Pierre Fontana, Walter A. Wuillemin, Adrian Schmidt, Lukas Graf, Bernhard Gerber, Cedric Bovet, Thomas C. Sauter, Nikolaus B. Binder, Michael Nagler
Summary: The study tested the clinical performance of a universal anti-Xa assay using an edoxaban calibrator, which accurately determined the concentrations of all anti-Xa inhibitors. The assay showed a high sensitivity and specificity in predicting drug concentrations.
Article
Immunology
Lucrezia Mencarelli, Laura Moi, Natacha Dewarrat, Matteo Monti, Lorenzo Alberio, Maxime Ringwald, Karolina Swierdzewska, Antiochos Panagiotis, Camillo Ribi
Summary: This article describes a rare case of Eosinophilic Granulomatosis with Polyangiitis (EGPA) occurring after the mRNA-1273 vaccine in a young asthmatic woman. The temporal relationship between vaccination and EGPA onset is intriguing, although a causal relationship cannot be proven. Cases of inflammatory diseases in the context of vaccination remain rare.
