Article
Medical Laboratory Technology
Caroline E. Franks, Jieli Li, Magen Martinez, Christopher W. Farnsworth, Patricia M. Jones, David G. Grenache, Qing H. Meng, Ann M. Gronowski
Summary: This study assessed the utility of 5 quantitative hCG immunoassays for use as tumor markers in trophoblastic and non-trophoblastic disease. Results suggest that all 5 immunoassays are adequate for using hCG as a tumor marker in gestational trophoblastic disease and select germ cell tumors. Further studies are needed to assess the utility of quantitative hCG as a tumor marker in other malignant diseases.
CLINICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Hatem Mohamed Elsheikh, Shadia Abdel-Hamid Fathy, Nahed Hassan Ebeid, Khaled Mohamed Sallam, Ahmed Sami El-bayoumy, Nagy Lahzy Mehany, Germine Mohsen Hamdy
Summary: The main objective of this study was to prepare and evaluate primary reagents for the liquid phase human chorionic gonadotropin-radioimmunoassay (HCG-RIA) kit. Polyclonal antibodies were found to be specific and valid, while monoclonal antibodies were more specific. The developed in-house HCG-RIA method showed high sensitivity, specificity, precision, and accuracy, making it suitable for quantifying HCG in human sera for pregnancy monitoring and diagnosis of gestational trophoblastic diseases.
JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
(2023)
Article
Chemistry, Analytical
Xue Wang, Huanyuan Wang, Yingying Sun, Zhe Liu, Na Wang
Summary: This study presents a liquid crystal biosensor using Au nanoparticles for signal amplification to detect HCG antigen in a micro and cost-effective manner. The sensor utilizes the unique properties of LC for HCG detection, providing excellent sensitivity and low detection limits.
Article
Biochemistry & Molecular Biology
Clara Lazzaretti, Valentina Secco, Elia Paradiso, Samantha Sperduti, Claudia Rutz, Annika Kreuchwig, Gerd Krause, Manuela Simoni, Livio Casarini
Summary: The study focused on identifying key residues in LHCGR that are likely evolved in humans to discriminate between specific binding of LH and hCG. Mutations within the receptor's leucine reach repeat 9 and 10 were found to lead to loss of discrimination ability, resembling the modulation of Lhr-mediated signaling patterns.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Nutrition & Dietetics
Kiwamu Noshiro, Takeshi Umazume, Rifumi Hattori, Soromon Kataoka, Takashi Yamada, Hidemichi Watari
Summary: This study found that serum ketone levels peak in the third trimester of pregnancy, but do not directly correlate with neonatal body shape. Weight loss during pregnancy may be related to morning sickness, with a higher pre-pregnancy body mass index leading to greater weight loss.
Article
Cell Biology
Hamideh P. Fallah, Maira S. Rodrigues, Maya Zanardini, Rafael H. Nobrega, Hamid R. Habibi
Summary: The study found that zGnih has a direct effect on spermatogenesis induced by both basal and gonadotropin (Lh and Fsh) in zebrafish testis. Specific concentrations of zGnih can significantly decrease the number of G0/G1 cells after 7 days in culture, with no significant impact on the proportion area of spermatogonia cell types. The research also revealed that zGnih reduces the proliferative activity of type A spermatogonia and increases the mitotic activity of type B spermatogonia.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2021)
Article
Medical Laboratory Technology
Christopher J. Mattiello, Douglas F. Stickle
Summary: We used image analysis to characterize the dose vs response curve of a qualitative serum hCG lateral flow immunoassay, which helped us understand the transition from negative to positive. The results showed that the curve could be described by a simple hyperbola equation, which was useful for verifying the assay's performance characteristics.
CLINICA CHIMICA ACTA
(2023)
Article
Peripheral Vascular Disease
Ruijuan Wang, Li Chen, Xiaobing Wang, Yanjun Liu
Summary: The aberrant increase of circulating β-human chorionic gonadotropin (?-HCG) at early stages of pregnancy is associated with predicting gestational hypertension. Inflammatory and oxidative stress markers play a significant role in severe pregnancy-induced hypertension during the perinatal stage.
MICROVASCULAR RESEARCH
(2021)
Review
Obstetrics & Gynecology
Jin Peng, Shangge Lv, Lin Liu, Shuai Feng, Naidong Xing
Summary: This systematic review highlights the importance of considering paraneoplastic secretion of beta-HCG in patients with lung neoplasms and positive pregnancy tests. Early recognition and appropriate management can prevent delays in diagnosis and treatment of malignancies in young women.
ARCHIVES OF GYNECOLOGY AND OBSTETRICS
(2021)
Article
Endocrinology & Metabolism
Dzhamilyat Abdulkhalikova, Eda Vrtacnik Bokal, Martin Stimpfel, Primoz Ciglar, Sara Korosec
Summary: This study evaluated the reproductive outcomes of GnRHa triggered cycles combined with modified luteal support in women with high ovarian response. The results showed no significant differences in pregnancy rate per embryo transfer, rate of early pregnancy loss, and live birth rate per pregnancy between the GnRHa and hCG trigger groups. However, the birth weight in the GnRHa trigger group was significantly lower.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Health Care Sciences & Services
Risa Fujishima, Kaoru Kawasaki, Kaori Moriuchi, Reona Shiro, Yoshie Yo, Noriomi Matsumura
Summary: This retrospective study compares early manual removal of placenta (MROP) and conservative management of retained products of conception (RPOC) after 34 weeks of gestation. The study found that while both methods had advantages and disadvantages, early MROP led to a shorter time for the placenta to disappear, but also resulted in lower hemoglobin levels after massive bleeding. Randomized controlled trials are necessary to determine the superior treatment option.
Article
Oncology
Zhihui Wu, Heng Xue, Miao Zheng, Huiyu Chen, Guolong Chen, Liangpu Xu
Summary: In this study, a sensitive sandwich-like electrochemical immunosensor of hCG was constructed using nanogold/graphene hybrids and ferrocene/gold nanoparticles as the sensing platform and signal amplifier, respectively. After optimizing various testing conditions, this method showed a wide linearity range and low detection limit for hCG analysis, suggesting its usefulness for early pregnancy testing and clinical diagnosis.
AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH
(2022)
Article
Chemistry, Analytical
Adrian Koterwa, Magdalena Bojko, Jacek Ryl, Krzysztof Lukaszuk, Kornelia Kozlowska, Wiktor Sieklicki, Sylwia Rodziewicz-Motowidlo, Pawel Niedzialkowski
Summary: This work describes the modification of a gold electrode to create an electrochemical biosensor capable of detecting human chorionic gonadotropin (hCG). The biosensor was obtained by modifying the gold electrode with cysteamine and oligopeptide (PPLRINRHILTR). The effectiveness of the biosensor was confirmed in human serum, and the results indicate that it is selective for the presence of hCG hormone.
Article
Cell Biology
Ilaria Dando, Cristian Andres Carmona-Carmona, Nicola Zampieri
Summary: hCG is a hormone that binds to LHR and plays roles in pregnancy and tumor development. Studies have shown conflicting results on the role of hCG in cancer. In vitro experiments without serum showed that hCG could promote cell proliferation in breast cancer cell lines.
Article
Clinical Neurology
Monique Peris, Susan M. Reid, Stephen Dobie, Leo Bonacquisto, Daisy A. Shepherd, David J. Amor
Summary: The study found that CP pregnancies had lower levels of PAPP-A and beta-hCG compared to control pregnancies. Abnormally low biomarker levels in CP pregnancies were associated with factors such as low Apgar scores, moderate preterm birth, and low birthweight.
DEVELOPMENTAL MEDICINE AND CHILD NEUROLOGY
(2021)
Review
Endocrinology & Metabolism
Fen-Ting Liu, Ze Wu, Jie Yan, Robert J. Norman, Rong Li
FRONTIERS IN ENDOCRINOLOGY
(2020)
Article
Health Care Sciences & Services
Jessica A. Grieger, Robert J. Norman
JOURNAL OF MEDICAL INTERNET RESEARCH
(2020)
Article
Obstetrics & Gynecology
Shuo Huang, Rui Wang, Hongmei Yan, Nannan Li, Haiyan Wang, Li Luo, Lina Wang, Robert J. Norman, Rong Li, Jie Qiao, Ben Willem J. Mol
Summary: The study aimed to explore the feasibility of comparing IUI with or without letrozole in couples with unexplained or mild male factor infertility. The results showed that there was no significant difference in outcomes between IUI with letrozole and natural cycle IUI, and patients were willing to participate in the randomized trial.
EUROPEAN JOURNAL OF OBSTETRICS & GYNECOLOGY AND REPRODUCTIVE BIOLOGY
(2021)
Article
Obstetrics & Gynecology
Robert J. Norman
Summary: Immunology and reproduction are closely connected in human physiology, but the mechanisms by which the uterus accepts and supports the fetus remain unclear. The use of unproven and expensive tests and therapies to alter immune mechanisms in reproductive medicine is becoming more common, highlighting the need for specialists to have a better understanding of immune physiology in reproduction and to exercise caution in the use of treatments until evidence-based data is available.
FERTILITY AND STERILITY
(2022)
Article
Obstetrics & Gynecology
Stephanie K. Y. Choi, Christos Venetis, William Ledger, Alys Havard, Katie Harris, Robert J. Norman, Louisa R. Jorm, Georgina M. Chambers
Summary: In a country with supportive funding for medically assisted reproduction (MAR) technologies, the proportion of MAR births increased from 5.1% in 2009 to 6.7% in 2017, with ART births increasing by 55% and stable contribution from OI/IUI births.
HUMAN REPRODUCTION
(2022)
Editorial Material
Obstetrics & Gynecology
Isobel G. Marshall, Robert J. Norman
FERTILITY AND STERILITY
(2022)
Editorial Material
Obstetrics & Gynecology
Robert J. Norman
Summary: Despite previous difficulties, recent advancements in understanding natural oocyte maturation, culture media, and clinical approaches have increased the potential for a wider use of in vitro maturation in human IVF.
FERTILITY AND STERILITY
(2023)
Editorial Material
Obstetrics & Gynecology
Robert John Norman, Rachel Morman, Helena J. Teede
FERTILITY AND STERILITY
(2023)
Article
Medicine, General & Internal
Christos Venetis, Stephanie K. Y. Choi, Louisa Jorm, Xian Zhang, William Ledger, Kei Lui, Alys Havard, Michael Chapman, Robert J. Norman, Georgina M. Chambers
Summary: This study investigated the role of underlying infertility and fertility treatment on the risks of congenital anomalies (CAs) in infants. The study found that infants conceived using assisted reproductive technologies (ARTs) had an increased risk for major genitourinary abnormalities, and intracytoplasmic sperm injection (ICSI) in couples without male infertility was associated with this risk.
ANNALS OF INTERNAL MEDICINE
(2023)
Letter
Obstetrics & Gynecology
Robert John Norman, Rachel Morman, Helena J. Teede
FERTILITY AND STERILITY
(2023)
Article
Obstetrics & Gynecology
Lulu Geng, Yan Yang, Yifeng Chen, Tingting Ye, Andong Qiu, Orhan Bukulmez, Ben W. Mol, Robert J. Norman, Xiaoming Teng, Jianjun Xiang, Miaoxin Chen
Summary: This study examines the impact of ambient temperature exposure on outcomes of IVF cycles and finds that both extreme cold and hot temperatures are associated with adverse pregnancy outcomes.
HUMAN REPRODUCTION
(2023)
Article
Obstetrics & Gynecology
D. Romualdi, S. Guideline Grp Unexplained Infertility, B. Ata, S. Bhattacharya, E. Bosch, M. Costello, K. Gersak, R. Homburg, M. Mincheva, R. J. Norman, T. Piltonen, S. Dos Santos-Ribeiro, D. Scicluna, S. Somers, S. K. Sunkara, H. R. Verhoeve, N. Le Clef
Summary: This study aimed to provide recommendations for the management of couples with unexplained infertility (UI) based on the best available evidence. The guideline outlined the definition, diagnosis, and treatment of UI, and formulated 52 recommendations. However, the efficacy, safety, costs, and risks of treatment options for UI have not been thoroughly evaluated.
HUMAN REPRODUCTION
(2023)
Review
Obstetrics & Gynecology
Robert J. Norman, Roger J. Hart
Summary: Human growth hormone is used as a co-gonadotrophin in assisted reproduction to improve outcomes in women with poor ovarian response. While some studies suggest improved ovarian response, there is still controversy regarding its impact on ongoing pregnancy and live birth rates.
THERAPEUTIC ADVANCES IN REPRODUCTIVE HEALTH
(2021)
Article
Health Care Sciences & Services
Erandi Hewawasam, Leah Brennan, Lynne Giles, Mary Louise Hull, Asha Short, Robert Norman, Alexia S. Pena
JMIR RESEARCH PROTOCOLS
(2020)
Review
Obstetrics & Gynecology
Moustafa A. Gadalla, Robert J. Norman, Chau T. Tay, Danielle S. Hiam, Angela Melder, Jyotsna Pundir, Shakila Thangaratinam, Helena J. Teede, Ben W. J. Mol, Lisa J. Moran
INTERNATIONAL JOURNAL OF FERTILITY & STERILITY
(2020)