Article
Chemistry, Analytical
Tian Tang, Pei Zhang, Siqi Li, Doudou Xu, Wei Li, Yuan Tian, Yu Jiao, Zunjian Zhang, Fengguo Xu
Summary: This study utilized microwave-assisted Tmt-PP derivatization and the QAMS strategy to improve the accuracy of LC-MS/MS determination of ACs, reducing reaction time and resolving the standard availability issue. The established workflow was applied to discover dysregulated ACs in xenograft colon cancer mice, with quantification results highly comparable to traditional methods.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Medicinal
Margaux Fresnais, Siwen Liang, Marius Breitkopf, Joshua Raoul Lindner, Emmanuelle Claude, Steven Pringle, Pavel A. Levkin, Konstantin Demir, Julia Benzel, Julia Sundheimer, Britta Statz, Kristian W. Pajtler, Stefan M. Pfister, Walter E. Haefeli, Juergen Burhenne, Remi Longuespee
Summary: Desorption/ionization-mass spectrometric methods are advantageous for the analysis of solid biological matrices. The development of drug quantification assays using DESI has been challenging, but new prototype source setups show promise for targeted quantitative analyses.
Article
Biochemistry & Molecular Biology
Margaux Fresnais, Siwen Liang, Deniz Seven, Nevena Prodanovic, Julia Sundheimer, Walter E. Haefeli, Juergen Burhenne, Remi Longuespee
Summary: The development and validation of desorption/ionization mass spectrometric assays for drug quantification in tissue sections is crucial for their widespread application in (clinical) pharmacology. Advances in desorption electrospray ionization have demonstrated its usefulness for developing targeted quantification methods that meet validation requirements. Factors such as desorption spot morphology, analytical time, and sample surface need to be considered for successful method development. This study highlights the importance of considering desorption kinetics during DESI analyses, which can help reduce analytical time, verify solvent-based drug extraction methods, and predict feasibility of imaging assays. These findings provide valuable guidance for future development of validated DESI-profiling and imaging methods.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Analytical
Chen Ling, Qiaofang Shi, Zhanpeng Wei, Jingjing Zhang, Junjie Hu, Jiying Pei
Summary: A rapid, environmentally friendly wooden-tip electrospray ionization mass spectrometry (ESI-MS) method was introduced for the determination of quinones in PM2.5, urine, and serum, showing high sensitivity and applicability in complex matrices without extensive sample preparation (LOD range: 0.22-1.48 ng).
Article
Biochemistry & Molecular Biology
Margaux Fresnais, Seda Karabulut, Yasmin Abou Zeed, Johannes Ungermann, Julia Benzel, Kristian W. Pajtler, Stefan M. Pfister, Walter E. Haefeli, Juergen Burhenne, Remi Longuespee
Summary: This article highlights the importance of using DI-MS approaches for rapid drug quantification in biological matrices and discusses the selection of internal standards for MALDI-MS assays using different instrumentations. The article also provides preliminary results of method development for the quantification of selinexor in microdialysis fluids with a stable isotopically labeled internal standard.
Review
Biochemical Research Methods
Lanaia Itala Louzeiro Maciel, Ricardo Alves Bernardo, Rafael Oliveira Martins, Almir Custodio Batista Jr, Joao Victor Ataide Oliveira, Andrea Rodrigues Chaves, Boniek Gontijo Vaz
Summary: The imaging of biological tissues using mass spectrometry imaging (MSI) techniques allows for the visualization of the distribution of various metabolites, drugs, lipids, and glycans in complex samples. MSI methods like DESI-MSI and MALDI-MSI provide high sensitivity and the ability to evaluate/visualize multiple analytes in a single sample, overcoming limitations of classical microscopy techniques. This review discusses the application of DESI-MSI and MALDI-MSI in evaluating exogenous and endogenous molecules in biological samples, providing technical insights and recent research findings.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Review
Pharmacology & Pharmacy
Manman Qin, Yuqing Qian, Lu Huang, Chao Zhong, Mingdong Li, Jun Yu, Huanwen Chen
Summary: Extraction electrospray ionization mass spectrometry (EESI-MS) enables effective ionization of trace molecules in complex samples without sample pretreatment, providing high sensitivity, selectivity, and response speed. It also allows for advanced functionalization of high-energy molecular species. This article systematically reviews the effects of operating conditions on the analytical performance of EESI-MS for various pharmaceutical compounds and introduces its potential application in molecular functionalization for drug discovery.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Biochemical Research Methods
Hiromori Murashima, Akimasa Fujihara
Summary: The study investigated chiral recognition between amino acids and monosaccharides in the gas phase as a model for chemical evolution in interstellar molecular clouds. Results showed that the optical properties of Trp in the clusters induced by 285-nm photoexcitation were useful for identifying and quantifying pentose enantiomers. Additionally, pentose enantiomeric excess in solution could be determined from relative abundances observed in a single product ion spectrum of 285-nm photoexcited hydrogen-bonded clusters of H+(L-Trp) and pentose.
ANALYTICAL BIOCHEMISTRY
(2021)
Article
Chemistry, Analytical
Chao Liu, Jiafa Zeng, Pablo Sinues, Mingliang Fang, Zhen Zhou, Xue Li
Summary: SESI-HRMS is a direct mass spectrometry technique that can identify trace VOCs in real time, but further development of absolute quantification methods is needed.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Margaux Fresnais, Juergen Burhenne, Walter E. Haefeli, Remi Longuespee
Summary: Desorption/ionization (DI) methods are crucial for rapid and sensitive drug quantification from solid samples. Validating these methods requires careful consideration of the specific characteristics of DI approaches compared to traditional LC-MS methods.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Shuanglong Wang, Shan Shan, Jian Yang, Junhui Zhou, Guofeng Wang
Summary: A triangular-shaped flat plastic substrate probe was prepared for direct ESI-MS analysis of untreated chemical and biological samples. Compared to conventional capillary ESI, this plastic probe offers advantages such as lower cost, higher sensitivity, lower sample consumption, longer signal duration, better reproducibility, and faster analysis speed.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Michael Goetze, Lukasz Polewski, Leila Bechtella, Kevin Pagel
Summary: This article introduces a 3D-printable nano-electrospray ionization (nano-ESI) source for Bruker instruments, which can be assembled by anyone with access to a 3D printer. The source can be fitted to any Bruker mass spectrometer with an ionBooster ESI source and only requires minor, reversible changes to the original Bruker hardware. The general utility of the source was demonstrated by recording high-resolution MS spectra of small molecules, intact proteins, as well as complex biological samples in negative and positive ion mode on two different Bruker instruments.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Multidisciplinary Sciences
Amanda Gisler, Kapil Dev Singh, Jiafa Zeng, Martin Osswald, Mo Awchi, Fabienne Decrue, Felix Schmidt, Noriane A. Sievi, Xing Chen, Jakob Usemann, Urs Frey, Malcolm Kohler, Xue Li, Pablo Sinues
Summary: Exhaled breath analysis provides valuable molecular-level information and has promising potential for precision medicine. However, the lack of validation in multicenter trials hinders the transition of breath tests into routine clinical practice. In this study, an interoperability framework was developed and applied, revealing a technical variability of approximately 20% and identifying a core signature of the human exhaled metabolome. The results showed high inter-subject variability for certain metabolic classes but relative stability in other regions, such as the TCA cycle. This framework and the overview of metabolic coverage will pave the way for future large-scale multicenter trials.
Article
Chemistry, Analytical
Liang Chen, Alireza Ghiasvand, Shing Chung Lam, Estrella Sanz Rodriguez, Peter C. Innis, Brett Paull
Summary: A thread-based isotachophoresis method coupled with desorption electrospray ionization mass spectrometry (TB-ITP-DESI-MS) was developed for clean-up, preconcentration, and determination of alkaloids in biological fluids. The TB-ITP platform components were fabricated using 3D printing, with a nylon thread as the electrophoresis substrate and a cotton knot as the trapping zone. The method showed improved signal to noise ratio compared to direct DESI-MS detection, making it a viable solution for the analysis of complex biological fluid samples.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biochemistry & Molecular Biology
Daiya Nagai, Akimasa Fujihara
Summary: Chemical properties of gas-phase hydrogen-bonded clusters were studied as a model for interstellar molecular clouds. Cold gas-phase hydrogen-bonded clusters of tryptophan enantiomers and disaccharide isomers were generated and analyzed using tandem mass spectrometry. The photoreactivity and quantification methods of the clusters were investigated, providing important insights into their composition and behavior.
CARBOHYDRATE RESEARCH
(2023)