Article
Pharmacology & Pharmacy
Alexie Mayor, Beatrice Thibert, Sylvain Huille, Fethi Bensaid, Renaud Respaud, Heloise Audat, Nathalie Heuze-Vourc'h
Summary: In the past decade, oral inhalation has been a popular research topic for direct treatment of respiratory diseases. The formulation of inhaled antibodies plays a crucial role in their stability and lung safety. This study reveals the significant impact of the buffering system on the stability of monoclonal antibodies during nebulization.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2022)
Article
Pharmacology & Pharmacy
Yue Liu, Hao Li, Zhen Yan, Lianshan Zhang, Piaoyang Sun
Summary: This study explores the mechanism of CDR fragmentation in therapeutic antibodies and identifies excipients that can control fragmentation. Trp100 oxidation is found to be correlated with fragment formation, and adding Trp can reduce the level of fragmentation. Moreover, substituting polysorbate 80 (PS80) with other surfactants, using a combination of antioxidants, or combining EDTA with PS80 can maintain the stability of the antibody.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2022)
Article
Biotechnology & Applied Microbiology
Yuanli Song, Hui Cai, Zhijun Tan, Nesredin Mussa, Zheng-Jian Li
Summary: This study demonstrates that therapeutic IgG1 and IgG4 antibodies display different sensitivity to the reducing agent TCEP and follow different pathways to form low molecular weight (LMW) species. The findings provide mechanistic insights into LMW formation of IgG1 and IgG4, which have implications for the selection of IgG1 or IgG4 in bispecific antibodies and cysteine-based antibody-drug conjugates.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Medicine, Research & Experimental
Yilue Zhang, Christian Schoeneich
Summary: In this study, a novel visible light-induced heavy chain fragmentation method of IgG1 mediated by an Fe(III)-containing histidine buffer was reported. The fragmentation involves oxidative cleavage and intrachain radical reactions. The presence of His is required for the cleavage, and it can be prevented by EDTA.
MOLECULAR PHARMACEUTICS
(2022)
Article
Oncology
Seung Won Choi, Hyun Ae Jung, Hee-Jin Cho, Tae Min Kim, Chul-Kee Park, Do-Hyun Nam, Se-Hoon Lee
Summary: This study evaluated the therapeutic efficacy of the novel anti-EGFR monoclonal antibody GC1118 in recurrent GBM patients with EGFR amplification. Although the study did not meet the primary endpoint, it was found that immune-related signatures were significantly upregulated in tumors with regression upon GC1118 therapy, indicating the potential immune-mediated antitumor efficacy of GC1118.
Article
Medicine, Research & Experimental
Zhou Tian, Feng Qian
Summary: The nature of ATP as amphiphilic and hydrotrope plays a role in maintaining solubility and preventing liquid-liquid phase separation of proteins, with ATP-MA1 static interaction driving phase separation. The attractive protein-protein interaction exclusively increased in the presence of ATP, altering protein surface properties and inducing LLPS of MA1. This ATP-induced LLPS was effectively eliminated by Mg2+ chelation with ATP to negate the static interaction.
MOLECULAR PHARMACEUTICS
(2021)
Article
Medicine, Research & Experimental
Yilue Zhang, Christian Schoeneich
Summary: In this study, a novel visible light-induced heavy chain fragmentation of IgG1 mediated by an Fe(III)-containing histidine buffer was reported. The IgG1 fragments with apparent molecular weights of around 130, 110, and 22 kDa were detected in photo-irradiated samples. The cleavage process was found to involve an oxidative cleavage at Thr259, and the presence of histidine was necessary for the fragmentation.
MOLECULAR PHARMACEUTICS
(2023)
Article
Biochemistry & Molecular Biology
Christina Bergonzo, J. Todd Hoopes, Zvi Kelman, D. Travis Gallagher
Summary: In this study, all-atom simulations were used to compare the dynamic behavior of four different structural variants of Fc antibody domain. The effects of hinge and glycans on stability were independently measured using umbrella sampling simulations. Principal component analysis was conducted to determine the free energy effects along the dominant mode of motion for Fc.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Review
Immunology
Zeineb Zian, S. P. Deo-Gracias Berry, Nazila Bahmaie, Dana Ghotbi, Ali Kashif, Manisha Madkaikar, Umair Ahmed Bargir, Hamisu Abdullahi, Haroon Khan, Gholamreza Azizi
Summary: The review provides information on the efficacy, dose schedules, and side effects of rituximab in the treatment of immune-mediated diseases, discussing its potential as a treatment regimen for autoimmune diseases, primary immunodeficiency diseases, and malignancies. Collaboration between basic medical researchers and clinical specialists is highlighted as crucial in considering the use of rituximab for various immune-mediated disorders.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Multidisciplinary Sciences
Anouk Smet, Joao Paulo Portela Catani, Tine Ysenbaert, Amanda Goncalves, Harry Kleanthous, Thorsten U. Vogel, Xavier Saelens, Emma R. Job
Summary: This study demonstrates that NA-specific antibodies can interfere with the replication of A(H1N1)pdm09 in human airway epithelial cells, blocking viral entry and egress. Furthermore, human sera with neuraminidase inhibition activity against A(H1N1)pdm09 N1 can also reduce H6N1 virus infection in HAE cells.
Article
Biology
Oi Wah Liew, Samantha S. M. Ling, Shera Lilyanna, Yue Zhou, Peipei Wang, Jenny P. C. Chong, Yan Xia Ng, Angeline E. S. Lim, Eliot R. Y. Leong, Qifeng Lin, Teck Kwang Lim, Qinsong Lin, Enoch M. W. Ng, Tuck Wah Ng, A. Mark Richards
Summary: Liew et al. present an efficient monoclonal antibody (mAb) production method, successfully developing mAbs against multiple epitopes on hANKRD1 in a single hybridoma production cycle. With rapid screening and validation techniques, this pipeline shows promise of general applicability for antibody panel generation.
COMMUNICATIONS BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Kyle W. Anderson, Christina Bergonzo, Kerry Scott, Ioannis L. Karageorgos, Elyssia S. Gallagher, Venkata S. Tayi, Michael Butler, Jeffrey W. Hudgens
Summary: Previous studies have proposed different models for stabilizing the Fc-Fc gamma RI immune complex. This study used multiple experimental methods to investigate the role of glycoprotein interactions between IgG1 glycans and the FG-loop of Fc gamma RIa in complex stabilization. The results showed that both intermolecular and intramolecular glycoprotein bonds contribute to the stability of the complex.
JOURNAL OF MOLECULAR BIOLOGY
(2022)
Article
Chemistry, Medicinal
Juan P. Rincon Pabon, Brent A. Kochert, Yan-Hui Liu, Douglas D. Richardson, David D. Weis
Summary: Affinity chromatography is widely used for antibody purification in biopharmaceutical production, and here we used hydrogen exchange-mass spectrometry to study the conformational changes in the NIST mAb upon binding with a protein A matrix. The results showed increased protection in certain regions of the antibody structure when bound to ProA, but this effect was not observed with lower ProA concentration or when ProA resin was used.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Article
Immunology
Luisa Kreft, Aloys Schepers, Miriam Hils, Kyra Swiontek, Andrew Flatley, Robert Janowski, Mohammadali Khan Mirzaei, Michael Dittmar, Neera Chakrapani, Mahesh S. Desai, Stefanie Eyerich, Li Deng, Dierk Niessing, Konrad Fischer, Regina Feederle, Simon Blank, Carsten B. Schmidt-Weber, Christiane Hilger, Tilo Biedermann, Caspar Ohnmacht
Summary: The alpha-Gal epitope plays a determining role in allergic reactions and rejections in xenotransplantation. Humans lack this carbohydrate due to evolutionary loss of the enzyme GGTA1. Although commensal bacteria have been suggested as a causal factor for the induction of anti-alpha-Gal antibodies, it is unclear whether they actually express the native alpha-Gal epitope.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemical Research Methods
Masakazu Fukuda, Melissa A. Graewert, Cy M. Jeffries, Dmitri I. Svergun, Tadao Yamazaki, Akiko Koga, Yuji Yamanaka
Summary: This study investigates the acidic variants of therapeutic monoclonal antibodies (mAbs) using a refolding approach. The results showed that these acidic variants are conformational variants with minor and localized conformational changes.
ANALYTICAL BIOCHEMISTRY
(2023)
Article
Oncology
Arjan Gower, Wei-Hsun Hsu, Shuo-Tse Hsu, Yisong Wang, Giuseppe Giaccone
MOLECULAR ONCOLOGY
(2016)
Article
Oncology
C. A. Carter, A. Rajan, C. Keen, E. Szabo, S. Khozin, A. Thomas, C. Brzezniak, U. Guha, L. A. Doyle, S. M. Steinberg, L. Xi, M. Raffeld, Y. Tomita, M. J. Lee, S. Lee, J. B. Trepel, K. L. Reckamp, S. Koehler, B. Gitlitz, R. Salgia, D. Gandara, E. Vokes, G. Giaccone
ANNALS OF ONCOLOGY
(2016)
Article
Pharmacology & Pharmacy
Deepa S. Subramaniam, Eiran A. Warner, Giuseppe Giaccone
EXPERT OPINION ON INVESTIGATIONAL DRUGS
(2017)
Article
Oncology
Pasi A. Janne, Alice T. Shaw, D. Ross Camidge, Giuseppe Giaccone, S. Martin Shreeve, Yiyun Tang, Zelanna Goldberg, Jean-Francois Martini, Huiping Xu, Leonard R. James, Benjamin J. Solomon
JOURNAL OF THORACIC ONCOLOGY
(2016)
Article
Oncology
Anna Teresa Alberobello, Yisong Wang, Frans Joseph Beerkens, Fabio Conforti, Justine N. McCutcheon, Guanhua Rao, Mark Raffeld, Jing Liu, Raneen Rahhal, Yu-Wen Zhang, Giuseppe Giaccone
JOURNAL OF THORACIC ONCOLOGY
(2016)
Review
Oncology
William D. Travis, Hisao Asamura, Alexander A. Bankier, Mary Beth Beasley, Frank Detterbeck, Douglas B. Flieder, Jin Mo Goo, Heber MacMahon, David Naidich, Andrew G. Nicholson, Charles A. Powell, Mathias Prokop, Ramon Rami-Porta, Valerie Rusch, Paul van Schil, Yasushi Yatabe
JOURNAL OF THORACIC ONCOLOGY
(2016)
Review
Oncology
Paul A. Bunn, John D. Minna, Alexander Augustyn, Adi F. Gazdar, Youcef Ouadah, Mark A. Krasnow, Anton Berns, Elisabeth Brambilla, Natasha Rekhtman, Pierre P. Massion, Matthew Niederst, Martin Peifer, Jun Yokota, Ramaswamy Govindan, John T. Poirier, Lauren A. Byers, Murry W. Wynes, David G. McFadden, David MacPherson, Christine L. Hann, Anna F. Farago, Caroline Dive, Beverly A. Teicher, Craig D. Peacock, Jane E. Johnson, Melanie H. Cobb, Hans -Guido Wendel, David Spigel, Julien Sage, Ping Yang, M. Catherine Pietanza, Lee M. Krug, John Heymach, Peter Ujhazy, Caicun Zhou, Koichi Goto, Afshin Dowlati, Camilla Laulund Christensen, Keunchil Park, Lawrence H. Einhorn, Martin J. Edelman, Giuseppe Giaccone, David E. Gerber, Ravi Salgia, Taofeek Owonikoko, Shakun Malik, Niki Karachaliou, David R. Gandara, Ben J. Slotman, Fiona Blackhall, Glenwood Goss, Roman Thomas, Charles M. Rudin, Fred R. Hirsch
JOURNAL OF THORACIC ONCOLOGY
(2016)
Article
Oncology
Peter Goldstraw, Kari Chansky, John Crowley, Ramon Rami-Porta, Hisao Asamura, Wilfried E. E. Eberhardt, Andrew G. Nicholson, Patti Groome, Alan Mitchell, Vanessa Bolejack
JOURNAL OF THORACIC ONCOLOGY
(2016)
Article
Oncology
Anish Thomas, Yuanbin Chen, Arlene Berman, David S. Schrump, Giuseppe Giaccone, Ira Pastan, David J. Venzon, David J. Liewehr, Seth M. Steinberg, Markku Miettinen, Raffit Hassan, Arun Rajan
Editorial Material
Oncology
Stephen V. Liu, Giuseppe Giaccone
NATURE REVIEWS CLINICAL ONCOLOGY
(2016)
Article
Oncology
Rong An, Yisong Wang, Donna Voeller, Arjan Gower, In-Kyu Kim, Yu-Wen Zhang, Giuseppe Giaccone
Article
Medical Informatics
Sandy Mohan, Munish Mehra, Maryrose Petrizzo, Terry Katz
THERAPEUTIC INNOVATION & REGULATORY SCIENCE
(2016)
Editorial Material
Oncology
Stephen V. Liu, Giuseppe Giaccone
TRANSLATIONAL CANCER RESEARCH
(2016)
Editorial Material
Oncology
Justine N. McCutcheon, Xiaoliang Zhao, Giuseppe Giaccone
TRANSLATIONAL CANCER RESEARCH
(2016)
Article
Medical Informatics
Andrew Della-Coletta, Terry Katz, Kathy Kupka, Sandy Mohan, Kamila A. Novak, Maryrose Petrizzo, Sarah Ann Silvers
THERAPEUTIC INNOVATION & REGULATORY SCIENCE
(2020)