Article
Hematology
Jeongmoo Han, Sunwon Kim, Hyun Jung Kim, Hyeong Soo Nam, Min Woo Lee, Joon Woo Song, Jin Won Kim, Hongki Yoo
Summary: Multispectral fluorescence lifetime imaging microscopy (FLIM) was used to investigate the complex composition of coronary artery and atheroma at the pixel level. Automated visualization of multiple plaque components without the need for histological staining and analysis was achieved using this FLIM strategy.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Levin Hafa, Louise Breideband, Lucas Ramirez Posada, Nuria Torras, Elena Martinez, Ernst H. K. Stelzer, Francesco Pampaloni
Summary: Tissue engineering is an important and widely applicable field that offers alternatives to animal models and enables tissue regeneration and organ transplantation. 3D bioprinting stands out for its flexibility and reproducibility. A study introduces an integrated fluorescent light sheet bioprinting and imaging system, which allows real-time monitoring of material crosslinking and cell activity, offering high speed, high resolution, and real-time characterization capabilities.
ADVANCED MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Nawar Sakr, Olga Glazova, Liudmila Shevkova, Nikita Onyanov, Samira Kaziakhmedova, Alena Shilova, Maria V. Vorontsova, Pavel Volchkov
Summary: Tissue autofluorescence in fixed tissue sections is a major issue in fluorescence microscopy. The intense intrinsic fluorescence emitted by the adrenal cortex interferes with fluorescent labels, resulting in poor-quality images. Various tissue treatment methods were evaluated for reducing autofluorescence, and TrueBlack (TM) Lipofuscin Autofluorescence Quencher and MaxBlock (TM) Autofluorescence Reducing Reagent Kit were found to be the most effective, reducing autofluorescence intensity by 89-93% and 90-95%, respectively. These treatments preserved specific fluorescence signals and tissue integrity, allowing reliable detection of fluorescent labels in the adrenal cortex tissue.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Optics
Yujun Tang, Gang Wen, Yong Liang, Linbo Wang, Jie Zhang, Hui LI
Summary: Deep learning is used to reconstruct super-resolution structured illumination microscopy (SR-SIM) images, effectively reducing photobleaching and phototoxicity. A proposed dynamic SIM imaging strategy records full raw images at the beginning and then only wide-field images, with a deep-learning-based reconstruction algorithm improving the quality of reconstructed SR images while reducing photobleaching and phototoxicity. This algorithm also has the capability to observe new structures not included during network training.
Article
Optics
Brendan M. Heffernan, Peter S. Riley, Omkar D. Supekar, Stephanie A. Meyer, Diego Restrepo, Mark E. Siemens, Emily A. Gibson, Juliet T. Gopinath
Summary: This article presents a solution for imaging sub-diffraction dynamics of neural nanostructures involved in learning and memory using a two-photon, fiber-coupled, stimulated emission depletion microscope. The capabilities of this technique are demonstrated through super-resolution imaging of mammalian cells.
Article
Optics
Florian Strohl, Daniel Henry Hansen, Mireia Nager Grifo, Asa Birna Birgisdottir
Summary: Multifocus microscopy enables capturing entire volumes with a single exposure. This scalable method incorporates optical sectioning and improves axial resolution capabilities, making it suitable for imaging dense samples such as cells and tissues.
Article
Ophthalmology
Katharina Bermond, Leon von der Emde, Ioana-Sandra Tarau, Leonie Bourauel, Rainer Heintzmann, Frank G. Holz, Christine A. Curcio, Kenneth R. Sloan, Thomas Ach
Summary: This study investigated the effect of age-related macular degeneration (AMD) on retinal pigment epithelium cells in humans. The results showed that AMD-affected cells displayed increased size and granule count, as well as decreased autofluorescence (AF).
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Tiffany M. Heaster, Alexa R. Heaton, Paul M. Sondel, Melissa C. Skala
Summary: Metabolic autofluorescence imaging was used to assess cell-level macrophage heterogeneity in response to normal and cancerous tissue microenvironments in vivo. Tumor-associated macrophages exhibited higher oxidation levels compared to dermal macrophages, with changes in NAD(P)H and FAD protein-binding activities.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Nanoscience & Nanotechnology
Wei Wang, Yat Ho Chan, SoYoung Kwon, Jamuna Tandukar, Ruixuan Gao
Summary: Expansion microscopy (ExM) is a recently developed nanoscale fluorescence imaging method that enhances resolution by physically expanding biological samples. This review focuses on the early conception and development of ExM, discussing various variations and challenges faced, as well as the potential to push the resolution and accuracy beyond current limits.
Article
Biochemical Research Methods
J. Brunet, C. L. Walsh, W. L. Wagner, A. Bellier, C. Werlein, S. Marussi, D. D. Jonigk, S. E. Verleden, M. Ackermann, Peter D. Lee, Paul Tafforeau
Summary: The authors present a protocol for preparing and mounting whole human organs for X-ray hierarchical phase-contrast tomography, resulting in high-resolution images. Imaging across different scales is crucial for understanding organ morphology and pathophysiological changes. The protocol enables scanning of large soft-tissue samples, including intact human organs, with enhanced contrast and reduced sample movement and bubble formation.
Article
Biochemical Research Methods
Biagio Mandracchia, Jeonghwan Son, Shu Jia
Summary: The study introduced an optofluidic scanning microscopy (OSM) system for super-resolution, live-cell imaging, utilizing multi-focal excitation through the innate fluidic motion of specimens to achieve effective resolution doubling, optical sectioning, and contrast enhancement. The system features minimal instrumental complexity and full compatibility with various microfluidic configurations, offering a promising super-resolution optofluidic paradigm for miniaturization and different levels of integration at the chip scale.
Article
Multidisciplinary Sciences
Grigorii Timin, Michel C. Milinkovitch
Summary: Imaging collagen network architecture is crucial for understanding tissue mechanical properties in developmental and cancer biology. This study presents a simple and robust method using 'Fast Green' dye for whole-mount collagen staining, enabling exceptional visualization of collagen 3D network via confocal or light-sheet microscopy, compatible with solvent-based tissue clearing and immunostaining.
Article
Optics
N. R. Subedi, S. Yaraghi, P. S. Jung, G. Kukal, A. G. McDonald, D. N. Christodoulides, A. E. Vasdekis
Summary: Research shows that digitally scanned Airy beams can improve the throughput rates in Raman imaging by an order of magnitude compared to traditional diffraction-limited beams, achieving high contrast and 1 micron axial resolution for three-dimensional imaging of microparticles. This method also achieves orders of magnitude lower irradiation density than coherent Raman imaging schemes, with faster speed and lower phototoxicity.
Article
Biochemical Research Methods
Petra Paie, Gianmaria Calisesi, Alessia Candeo, Andrea Comi, Federico Sala, Francesco Ceccarelli, Ada De Luigi, Pietro Veglianese, Korbinian Muhlberger, Michael Fokine, Gianluca Valentini, Roberto Osellame, Mark Neil, Andrea Bassi, Francesca Bragheri
Summary: Heterogeneity investigation at the single-cell level is crucial in clinical research. The combination of light sheet fluorescence microscopy and structured illumination microscopy in an optofluidic platform enables high throughput super-resolution imaging, allowing comprehensive evaluation of cellular heterogeneity at high resolution.
Review
Chemistry, Multidisciplinary
Jiwoong Kwon, Mohamed Saleh Elgawish, Sang-Hee Shim
Summary: Photobleaching is a major limitation in super-resolution microscopy, but it can be overcome by developing new probes, chemical environments, and utilizing the on-off transitions of fluorescence. These efforts have the potential to enhance resolution and extend the observation time in super-resolution microscopy.
Review
Otorhinolaryngology
K. Thomas Robbins, Asterios Triantafyllou, Carlos Suarez, Fernando Lopez, Jennifer L. Hunt, PrimoI Strojan, Michelle D. Williams, Boudewijn J. M. Braakhuis, Remco de Bree, Michael L. Hinni, Luiz P. Kowalski, Alessandra Rinaldo, Juan P. Rodrigo, Vincent Vander Poorten, Lain J. Nixon, Robert P. Takes, Carl E. Silver, Alfio Ferlito
AURIS NASUS LARYNX
(2019)
Article
Oncology
Nikhil S. Chari, Cristina Ivan, Xiandong Le, Jinzhong Li, Ainiwaer Mijiti, Ameeta A. Patel, Abdullah A. Osman, Christine B. Peterson, Michelle D. Williams, Curtis R. Pickering, Carlos Caulin, Jeffrey N. Myers, George A. Calin, Stephen Y. Lai
JNCI-JOURNAL OF THE NATIONAL CANCER INSTITUTE
(2020)
Article
Pathology
Michelle D. Williams, Ron A. DeLellis, Lori A. Erickson, Ruta Gupta, Sarah J. Johnson, Kaori Kameyama, Sonali Natu, Tony Ng, Aurel Perren, Nancy D. Perrier, Raja R. Seethala, Anthony J. Gill
Summary: Standardized pathologic reporting improves patient care and prognosis determination for cancers. The International Collaboration on Cancer Reporting (ICCR) aims to develop and disseminate standardized data sets globally, providing essential resources for documenting rare tumors.
Article
Immunology
Xerxes Pundole, Amy Little Jones, Michael T. Tetzlaff, Michelle D. Williams, William A. Murphy, Adegbenga Otun, Ryan P. Goepfert, Michael A. Davies
Article
Multidisciplinary Sciences
Megan Heenan, Jose D. Rojas, Z. Maria Oden, Rebecca Richards-Kortum
Article
Chemistry, Analytical
Mary E. Natoli, Megan M. Chang, Kathryn A. Kundrod, Jackson B. Coole, Gladstone E. Airewele, Venee N. Tubman, Rebecca R. Richards-Kortum
Summary: A rapid and low-cost nucleic acid test for detecting the point mutation in SCD has been developed in this study, showing high sensitivity and specificity with a turnaround time of less than 30 minutes. The test can be performed near the point of care and may be adapted for detecting other disease-causing point mutations in genomic DNA.
ANALYTICAL CHEMISTRY
(2021)
Article
Oncology
Rozita Bagheri-Yarmand, Ramona Dadu, Lei Ye, Yaashmin Shiny Jebaraj, Jade A. Martinez, Junsheng Ma, Rohinton S. Tarapore, Joshua E. Allen, Steven Sherman, Michelle D. Williams, Robert F. Gagel
Summary: Gain-of-function mutations in the receptor tyrosine kinase RET drive medullary thyroid carcinoma (MTC) by preventing apoptosis through ATF4 inhibition. Depletion of RET decreases IGFBP2 and VEGFR2 expression in MTC cells, affecting cell survival and migration. Treatment with ONC201 decreases tumor growth and cell proliferation by regulating RET, VEGFR2, and IGFBP2 expression.
MOLECULAR CANCER THERAPEUTICS
(2021)
Editorial Material
Chemistry, Multidisciplinary
Emilie Newsham, Rebecca Richards-Kortum
ACS CENTRAL SCIENCE
(2021)
Article
Engineering, Biomedical
Catherine E. Majors, Michal E. Pawlowski, Tomasz S. Tkaczyk, Queen Dube, Rebecca Richards-Kortum
Summary: The study demonstrates that LeukoScope can assist in measuring WBC counts in pediatric patients but faces challenges in accurately assessing WBC counts in neonatal patients.
ANNALS OF BIOMEDICAL ENGINEERING
(2021)
Article
Pediatrics
Jennifer Carns, Sara Liaghati-Mobarhan, Aba Asibon, Alfred Chalira, Norman Lufesi, Elizabeth Molyneux, Maria Z. Oden, Rebecca Richards-Kortum, Kondwani Kawaza
Summary: The study revealed a significant improvement in survival rates for neonates weighing 1.0-1.3 kg after the implementation of CPAP in Malawi government hospitals, with the improvement sustained during the follow-up period. This demonstrates the effectiveness of a nurse-led CPAP program in improving outcomes for this neonatal population.
ARCHIVES OF DISEASE IN CHILDHOOD
(2022)
Article
Engineering, Biomedical
Jesse K. Adams, Dong Yan, Jimin Wu, Vivek Boominathan, Sibo Gao, Alex Rodriguez, Soonyoung Kim, Jennifer Carns, Rebecca Richards-Kortum, Caleb Kemere, Ashok Veeraraghavan, Jacob T. Robinson
Summary: Lensless microscopy with a designed phase mask allows for imaging of biological tissue in vivo, overcoming the limitations of existing techniques. The low-cost and small form factor of this lensless imaging method makes it suitable for clinical use in inaccessible areas of the body.
NATURE BIOMEDICAL ENGINEERING
(2022)
Article
Engineering, Biomedical
Huayu Hou, Ruchika Mitbander, Yubo Tang, Ahad Azimuddin, Jennifer Carns, Richard A. Schwarz, Rebecca R. Richards-Kortum
Summary: This article reviews the recent development and application of low-cost optical imaging technologies, emphasizing the integration of artificial intelligence to improve in vivo cancer detection accuracy in low-resource settings. The article also discusses the challenges and future prospects of applying optical imaging technologies in clinical practice.
CURRENT OPINION IN BIOMEDICAL ENGINEERING
(2023)
Article
Pediatrics
Sonia E. Sosa Saenz, Mary Kate Hardy, Megan Heenan, Z. Maria Oden, Rebecca Richards-Kortum, Queen Dube, Kondwani Kawaza
BMJ PAEDIATRICS OPEN
(2020)
Article
Chemistry, Multidisciplinary
Catherine E. Majors, Michal E. Pawlowski, Daniel C. Burke, Tomasz S. Tkaczyk, Alyssa Rieber, Rebecca Richards-Kortum
Article
Engineering, Biomedical
Sonia Parra, Eduardo Carranza, Jackson Coole, Brady Hunt, Chelsey Smith, Pelham Keahey, Mauricio Maza, Kathleen Schmeler, Rebecca Richards-Kortum
IEEE JOURNAL OF TRANSLATIONAL ENGINEERING IN HEALTH AND MEDICINE
(2020)